(Linear) (Six-base) MAPSORT of: KIEE2442.seq Check: 1049 from: 1 to: 5868 KIEE2442.orf With 182 enzymes: SgfI * February 19, 2013 13:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2721 3408 4074 5868 Size: 2721 687 666 1794 Fragments arranged by size: 2721 1794 687 666 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 715 2778 3743 3867 4698 5482 5868 Size: 715 2063 965 124 831 784 386 Fragments arranged by size: 2063 965 831 784 715 386 124 AflII C'TTAA_G Cuts at: 0 3607 4678 5868 Size: 3607 1071 1190 Fragments arranged by size: 3607 1190 1071 AflIII A'CryG_T Cuts at: 0 2558 4172 4178 4919 5144 5225 5868 Size: 2558 1614 6 741 225 81 643 Fragments arranged by size: 2558 1614 741 643 225 81 6 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 101 202 5868 Size: 101 101 5666 Fragments arranged by size: 5666 101 101 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 438 470 1234 1266 5868 Size: 438 32 764 32 4602 Fragments arranged by size: 4602 764 438 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 679 1095 2804 2833 3148 5566 5868 Size: 679 416 1709 29 315 2418 302 Fragments arranged by size: 2418 1709 679 416 315 302 29 ApaI G_GGCC'C Cuts at: 0 327 714 1821 5024 5867 5868 Size: 327 387 1107 3203 843 1 Fragments arranged by size: 3203 1107 843 387 327 1 ApoI r'AATT_y Cuts at: 0 4 4517 5868 Size: 4 4513 1351 Fragments arranged by size: 4513 1351 4 AvaI C'yCGr_G Cuts at: 0 320 1264 1870 4136 5868 Size: 320 944 606 2266 1732 Fragments arranged by size: 2266 1732 944 606 320 AvrII C'CTAG_G Cuts at: 0 2424 5433 5868 Size: 2424 3009 435 Fragments arranged by size: 3009 2424 435 BamHI G'GATC_C Cuts at: 0 16 1989 2747 4775 5868 Size: 16 1973 758 2028 1093 Fragments arranged by size: 2028 1973 1093 758 16 BanI G'GyrC_C Cuts at: 0 3826 5059 5868 Size: 3826 1233 809 Fragments arranged by size: 3826 1233 809 BanII G_rGCy'C Cuts at: 0 209 233 327 714 1408 1606 1821 2006 Size: 209 24 94 387 694 198 215 185 Cuts at: 2006 2071 2911 2921 3142 3780 4351 5024 5083 Size: 65 840 10 221 638 571 673 59 Cuts at: 5083 5204 5867 5868 Size: 121 663 1 Fragments arranged by size: 840 694 673 663 638 571 387 221 215 209 198 185 121 94 65 59 24 10 1 BbeI G_GCGC'C Cuts at: 0 3830 5868 Size: 3830 2038 BbsI GAAGACnn'nnnn_ Cuts at: 0 468 1421 1783 2130 2480 2517 3521 3567 Size: 468 953 362 347 350 37 1004 46 Cuts at: 3567 4545 5558 5868 Size: 978 1013 310 Fragments arranged by size: 1013 1004 978 953 468 362 350 347 310 46 37 BbvCI CC'TCA_GC Cuts at: 0 2999 3224 4999 5645 5868 Size: 2999 225 1775 646 223 Fragments arranged by size: 2999 1775 646 225 223 BciVI GTATCCnnnnn_n' Cuts at: 0 2542 3378 5868 Size: 2542 836 2490 Fragments arranged by size: 2542 2490 836 BclI T'GATC_A Cuts at: 0 353 4469 5868 Size: 353 4116 1399 Fragments arranged by size: 4116 1399 353 BfrBI ATG'CAT Cuts at: 0 3356 5868 Size: 3356 2512 BglI GCCn_nnn'nGGC Cuts at: 0 322 1601 1637 1825 2487 5868 Size: 322 1279 36 188 662 3381 Fragments arranged by size: 3381 1279 662 322 188 36 BglII A'GATC_T Cuts at: 0 618 5868 Size: 618 5250 BlpI GC'TnA_GC Cuts at: 0 3490 5868 Size: 3490 2378 Bme1580I G_kGCm'C Cuts at: 0 327 714 1821 1894 2358 2993 5024 5639 Size: 327 387 1107 73 464 635 2031 615 Cuts at: 5639 5867 5868 Size: 228 1 Fragments arranged by size: 2031 1107 635 615 464 387 327 228 73 1 BmgBI CAC'GTC Cuts at: 0 3191 4976 5868 Size: 3191 1785 892 Fragments arranged by size: 3191 1785 892 BmrI ACTGGGnnnn_n' Cuts at: 0 2368 5868 Size: 2368 3500 BmtI G_CTAG'C Cuts at: 0 1297 5868 Size: 1297 4571 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 256 1601 1668 1757 2074 2844 2933 3577 Size: 256 1345 67 89 317 770 89 644 Cuts at: 3577 5185 5402 5868 Size: 1608 217 466 Fragments arranged by size: 1608 1345 770 644 466 317 256 217 89 89 67 Bpu10I CC'TnA_GC Cuts at: 0 2834 2999 3224 3305 4999 5645 5868 Size: 2834 165 225 81 1694 646 223 Fragments arranged by size: 2834 1694 646 225 223 165 81 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1882 1945 5868 Size: 1882 63 3923 Fragments arranged by size: 3923 1882 63 BsaI GGTCTCn'nnnn_ Cuts at: 0 1577 2988 5230 5868 Size: 1577 1411 2242 638 Fragments arranged by size: 2242 1577 1411 638 BsaAI yAC'GTr Cuts at: 0 548 5868 Size: 548 5320 BsaBI GATnn'nnATC Cuts at: 0 245 5868 Size: 245 5623 BsaHI Gr'CG_yC Cuts at: 0 3827 5868 Size: 3827 2041 BsaWI w'CCGG_w Cuts at: 0 51 266 1274 1520 5868 Size: 51 215 1008 246 4348 Fragments arranged by size: 4348 1008 246 215 51 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1234 1264 1666 1696 3229 3259 5467 5497 Size: 1234 30 402 30 1533 30 2208 30 Cuts at: 5497 5868 Size: 371 Fragments arranged by size: 2208 1533 1234 402 371 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1216 1888 3237 3844 4324 4987 5203 5491 Size: 1216 672 1349 607 480 663 216 288 Cuts at: 5491 5662 5665 5868 Size: 171 3 203 Fragments arranged by size: 1349 1216 672 663 607 480 288 216 203 171 3 BseYI C'CCAG_C Cuts at: 0 158 210 1614 1669 2229 2816 4985 5082 Size: 158 52 1404 55 560 587 2169 97 Cuts at: 5082 5868 Size: 786 Fragments arranged by size: 2169 1404 786 587 560 158 97 55 52 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 5584 5868 Size: 5584 284 BsiEI CG_ry'CG Cuts at: 0 4982 5868 Size: 4982 886 BsiHKAI G_wGCw'C Cuts at: 0 209 1136 2006 2057 2911 2951 3452 5197 Size: 209 927 870 51 854 40 501 1745 Cuts at: 5197 5868 Size: 671 Fragments arranged by size: 1745 927 870 854 671 501 209 51 40 BsmI GAATG_Cn' Cuts at: 0 1756 3356 5686 5868 Size: 1756 1600 2330 182 Fragments arranged by size: 2330 1756 1600 182 BsmBI CGTCTCn'nnnn_ Cuts at: 0 3181 5868 Size: 3181 2687 Bsp1286I G_dGCh'C Cuts at: 0 209 233 327 714 1136 1408 1606 1821 Size: 209 24 94 387 422 272 198 215 Cuts at: 1821 1894 2006 2057 2071 2358 2911 2921 2951 Size: 73 112 51 14 287 553 10 30 Cuts at: 2951 2993 3142 3452 3780 4351 5024 5083 5197 Size: 42 149 310 328 571 673 59 114 Cuts at: 5197 5204 5639 5867 5868 Size: 7 435 228 1 Fragments arranged by size: 673 571 553 435 422 387 328 310 287 272 228 215 209 198 149 114 112 94 73 59 51 42 30 24 14 10 7 1 BspEI T'CCGG_A Cuts at: 0 51 1274 5868 Size: 51 1223 4594 Fragments arranged by size: 4594 1223 51 BspHI T'CATG_A Cuts at: 0 1055 3479 3842 4301 4571 4799 5868 Size: 1055 2424 363 459 270 228 1069 Fragments arranged by size: 2424 1069 1055 459 363 270 228 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2721 2735 2789 3408 4074 5375 5868 Size: 2721 14 54 619 666 1301 493 Fragments arranged by size: 2721 1301 666 619 493 54 14 BsrBI CCG'CTC Cuts at: 0 1433 4765 5606 5868 Size: 1433 3332 841 262 Fragments arranged by size: 3332 1433 841 262 BsrDI GCAATG_nn' Cuts at: 0 939 1835 2147 2882 2993 4067 4649 5061 Size: 939 896 312 735 111 1074 582 412 Cuts at: 5061 5150 5868 Size: 89 718 Fragments arranged by size: 1074 939 896 735 718 582 412 312 111 89 BsrFI r'CCGG_y Cuts at: 0 4981 5868 Size: 4981 887 BsrGI T'GTAC_A Cuts at: 0 4175 5147 5868 Size: 4175 972 721 Fragments arranged by size: 4175 972 721 BssSI C'ACGA_G Cuts at: 0 387 5868 Size: 387 5481 BstAPI GCAn_nnn'nTGC Cuts at: 0 1290 1762 2804 3431 4955 5868 Size: 1290 472 1042 627 1524 913 Fragments arranged by size: 1524 1290 1042 913 627 472 BstBI TT'CG_AA Cuts at: 0 2078 3747 5868 Size: 2078 1669 2121 Fragments arranged by size: 2121 2078 1669 BstEII G'GTnAC_C Cuts at: 0 4066 5868 Size: 4066 1802 BstXI CCAn_nnnn'nTGG Cuts at: 0 2043 2379 2758 4482 5844 5868 Size: 2043 336 379 1724 1362 24 Fragments arranged by size: 2043 1724 1362 379 336 24 BstYI r'GATC_y Cuts at: 0 16 240 246 558 618 629 937 1150 Size: 16 224 6 312 60 11 308 213 Cuts at: 1150 1989 2747 3276 3299 3499 4775 5390 5868 Size: 839 758 529 23 200 1276 615 478 Fragments arranged by size: 1276 839 758 615 529 478 312 308 224 213 200 60 23 16 11 6 Bsu36I CC'TnA_GG Cuts at: 0 1096 1550 3212 3281 3292 5868 Size: 1096 454 1662 69 11 2576 Fragments arranged by size: 2576 1662 1096 454 69 11 BtgI C'CryG_G Cuts at: 0 624 1190 2048 2058 3400 3539 4375 5183 Size: 624 566 858 10 1342 139 836 808 Cuts at: 5183 5609 5868 Size: 426 259 Fragments arranged by size: 1342 858 836 808 624 566 426 259 139 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2640 2946 5868 Size: 2640 306 2922 Fragments arranged by size: 2922 2640 306 BtsI GCAGTG_nn' Cuts at: 0 951 1512 2894 3501 3769 4783 5162 5394 Size: 951 561 1382 607 268 1014 379 232 Cuts at: 5394 5868 Size: 474 Fragments arranged by size: 1382 1014 951 607 561 474 379 268 232 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1883 1918 4141 4176 5868 Size: 1883 35 2223 35 1692 Fragments arranged by size: 2223 1883 1692 35 35 DraI TTT'AAA Cuts at: 0 4098 5868 Size: 4098 1770 DraIII CAC_nnn'GTG Cuts at: 0 726 2018 5868 Size: 726 1292 3850 Fragments arranged by size: 3850 1292 726 DrdI GACnn_nn'nnGTC Cuts at: 0 844 1785 1911 5868 Size: 844 941 126 3957 Fragments arranged by size: 3957 941 844 126 EaeI y'GGCC_r Cuts at: 0 365 1530 1629 2045 2237 2297 2381 2580 Size: 365 1165 99 416 192 60 84 199 Cuts at: 2580 2760 3766 4547 4740 4766 4979 5180 5868 Size: 180 1006 781 193 26 213 201 688 Fragments arranged by size: 1165 1006 781 688 416 365 213 201 199 193 192 180 99 84 60 26 EagI C'GGCC_G Cuts at: 0 4979 5868 Size: 4979 889 EarI CTCTTCn'nnn_ Cuts at: 0 137 805 1664 3910 4717 4825 4966 5868 Size: 137 668 859 2246 807 108 141 902 Fragments arranged by size: 2246 902 859 807 668 141 137 108 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1039 4714 5868 Size: 1039 3675 1154 Fragments arranged by size: 3675 1154 1039 EcoICRI GAG'CTC Cuts at: 0 207 2004 2909 5868 Size: 207 1797 905 2959 Fragments arranged by size: 2959 1797 905 207 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 256 715 1601 1668 1757 2074 2778 2844 Size: 256 459 886 67 89 317 704 66 Cuts at: 2844 2933 3577 3743 3867 4698 5185 5402 5482 Size: 89 644 166 124 831 487 217 80 Cuts at: 5482 5868 Size: 386 Fragments arranged by size: 886 831 704 644 487 459 386 317 256 217 166 124 89 89 80 67 66 EcoNI CCTnn'n_nnAGG Cuts at: 0 5209 5868 Size: 5209 659 EcoO109I rG'GnC_Cy Cuts at: 0 324 710 711 1260 1639 2801 2935 3165 Size: 324 386 1 549 379 1162 134 230 Cuts at: 3165 3216 5020 5021 5868 Size: 51 1804 1 847 Fragments arranged by size: 1804 1162 847 549 386 379 324 230 134 51 1 1 EcoRI G'AATT_C Cuts at: 0 4 5868 Size: 4 5864 EcoRV GAT'ATC Cuts at: 0 888 1539 5868 Size: 888 651 4329 Fragments arranged by size: 4329 888 651 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1800 5868 Size: 1800 4068 FspI TGC'GCA Cuts at: 0 3424 5868 Size: 3424 2444 HaeII r_GCGC'y Cuts at: 0 3830 5868 Size: 3830 2038 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1303 1335 1408 1440 2992 3024 3229 3261 Size: 1303 32 73 32 1552 32 205 32 Cuts at: 3261 5296 5328 5375 5407 5644 5676 5868 Size: 2035 32 47 32 237 32 192 Fragments arranged by size: 2035 1552 1303 237 205 192 73 47 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1167 4044 4146 5142 5796 5868 Size: 1167 2877 102 996 654 72 Fragments arranged by size: 2877 1167 996 654 102 72 HindIII A'AGCT_T Cuts at: 0 3548 4427 5868 Size: 3548 879 1441 Fragments arranged by size: 3548 1441 879 KasI G'GCGC_C Cuts at: 0 3826 5868 Size: 3826 2042 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2995 5432 5868 Size: 2995 2437 436 Fragments arranged by size: 2995 2437 436 MscI TGG'CCA Cuts at: 0 1631 2047 2239 2299 2383 2582 2762 5182 Size: 1631 416 192 60 84 199 180 2420 Cuts at: 5182 5868 Size: 686 Fragments arranged by size: 2420 1631 686 416 199 192 180 84 60 MslI CAynn'nnrTG Cuts at: 0 373 1505 1951 2026 2041 2047 3478 3802 Size: 373 1132 446 75 15 6 1431 324 Cuts at: 3802 3895 4300 4444 4477 4480 4576 4636 5155 Size: 93 405 144 33 3 96 60 519 Cuts at: 5155 5842 5868 Size: 687 26 Fragments arranged by size: 1431 1132 687 519 446 405 373 324 144 96 93 75 60 33 26 15 6 3 MspA1I CmG'CkG Cuts at: 0 162 186 2486 3096 3455 4126 5868 Size: 162 24 2300 610 359 671 1742 Fragments arranged by size: 2300 1742 671 610 359 162 24 NaeI GCC'GGC Cuts at: 0 4983 5868 Size: 4983 885 NarI GG'CG_CC Cuts at: 0 3827 5868 Size: 3827 2041 NcoI C'CATG_G Cuts at: 0 1190 2048 2058 3400 5609 5868 Size: 1190 858 10 1342 2209 259 Fragments arranged by size: 2209 1342 1190 858 259 10 NdeI CA'TA_TG Cuts at: 0 600 2593 5868 Size: 600 1993 3275 Fragments arranged by size: 3275 1993 600 NgoMIV G'CCGG_C Cuts at: 0 4981 5868 Size: 4981 887 NheI G'CTAG_C Cuts at: 0 1293 5868 Size: 1293 4575 NsiI A_TGCA'T Cuts at: 0 3358 5868 Size: 3358 2510 NspI r_CATG'y Cuts at: 0 2496 2562 3801 4152 4182 4923 4953 5148 Size: 2496 66 1239 351 30 741 30 195 Cuts at: 5148 5229 5868 Size: 81 639 Fragments arranged by size: 2496 1239 741 639 351 195 81 66 30 30 PasI CC'CwG_GG Cuts at: 0 2939 3180 5860 5868 Size: 2939 241 2680 8 Fragments arranged by size: 2939 2680 241 8 PciI A'CATG_T Cuts at: 0 2558 4178 4919 5144 5225 5868 Size: 2558 1620 741 225 81 643 Fragments arranged by size: 2558 1620 741 643 225 81 PflMI CCAn_nnn'nTGG Cuts at: 0 2306 3148 3650 5400 5868 Size: 2306 842 502 1750 468 Fragments arranged by size: 2306 1750 842 502 468 PfoI T'CCnGG_A Cuts at: 0 1253 1578 5868 Size: 1253 325 4290 Fragments arranged by size: 4290 1253 325 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1234 1266 5868 Size: 1234 32 4602 Fragments arranged by size: 4602 1234 32 PpuMI rG'GwC_Cy Cuts at: 0 2801 3165 3216 5868 Size: 2801 364 51 2652 Fragments arranged by size: 2801 2652 364 51 PspOMI G'GGCC_C Cuts at: 0 323 710 1817 5020 5863 5868 Size: 323 387 1107 3203 843 5 Fragments arranged by size: 3203 1107 843 387 323 5 PspXI vC'TCGA_Gb Cuts at: 0 1870 5868 Size: 1870 3998 PstI C_TGCA'G Cuts at: 0 2732 3101 4085 5868 Size: 2732 369 984 1783 Fragments arranged by size: 2732 1783 984 369 PvuII CAG'CTG Cuts at: 0 162 186 3096 3455 4126 5868 Size: 162 24 2910 359 671 1742 Fragments arranged by size: 2910 1742 671 359 162 24 SacI G_AGCT'C Cuts at: 0 209 2006 2911 5868 Size: 209 1797 905 2957 Fragments arranged by size: 2957 1797 905 209 SapI GCTCTTCn'nnn_ Cuts at: 0 4717 5868 Size: 4717 1151 SbfI CC_TGCA'GG Cuts at: 0 2732 5868 Size: 2732 3136 ScaI AGT'ACT Cuts at: 0 3637 4324 4597 5868 Size: 3637 687 273 1271 Fragments arranged by size: 3637 1271 687 273 SexAI A'CCwGG_T Cuts at: 0 1160 2633 5868 Size: 1160 1473 3235 Fragments arranged by size: 3235 1473 1160 SfcI C'TryA_G Cuts at: 0 255 1181 2728 3097 4081 5868 Size: 255 926 1547 369 984 1787 Fragments arranged by size: 1787 1547 984 926 369 255 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1637 5868 Size: 1637 4231 SfoI GGC'GCC Cuts at: 0 3828 5868 Size: 3828 2040 SmaI CCC'GGG Cuts at: 0 4138 5868 Size: 4138 1730 SmlI C'TyrA_G Cuts at: 0 1264 1861 1870 1960 3607 4678 5868 Size: 1264 597 9 90 1647 1071 1190 Fragments arranged by size: 1647 1264 1190 1071 597 90 9 SpeI A'CTAG_T Cuts at: 0 1720 5868 Size: 1720 4148 SphI G_CATG'C Cuts at: 0 3801 4953 5868 Size: 3801 1152 915 Fragments arranged by size: 3801 1152 915 SspI AAT'ATT Cuts at: 0 3718 5868 Size: 3718 2150 StuI AGG'CCT Cuts at: 0 638 692 1472 5581 5780 5868 Size: 638 54 780 4109 199 88 Fragments arranged by size: 4109 780 638 199 88 54 StyI C'CwwG_G Cuts at: 0 562 782 1190 2048 2058 2424 2822 2867 Size: 562 220 408 858 10 366 398 45 Cuts at: 2867 3400 4769 5433 5609 5868 Size: 533 1369 664 176 259 Fragments arranged by size: 1369 858 664 562 533 408 398 366 259 220 176 45 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1606 1941 3147 3172 3603 3883 5868 Size: 1606 335 1206 25 431 280 1985 Fragments arranged by size: 1985 1606 1206 431 335 280 25 TatI w'GTAC_w Cuts at: 0 3560 3635 4175 4187 4322 4595 5147 5709 Size: 3560 75 540 12 135 273 552 562 Cuts at: 5709 5868 Size: 159 Fragments arranged by size: 3560 562 552 540 273 159 135 75 12 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2333 2365 3381 3413 5868 Size: 2333 32 1016 32 2455 Fragments arranged by size: 2455 2333 1016 32 32 Tth111I GACn'n_nGTC Cuts at: 0 3571 5868 Size: 3571 2297 XbaI T'CTAG_A Cuts at: 0 243 5868 Size: 243 5625 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 958 1333 4341 5868 Size: 958 375 3008 1527 Fragments arranged by size: 3008 1527 958 375 XhoI C'TCGA_G Cuts at: 0 1264 1870 5868 Size: 1264 606 3998 Fragments arranged by size: 3998 1264 606 XmaI C'CCGG_G Cuts at: 0 4136 5868 Size: 4136 1732 XmnI GAAnn'nnTTC Cuts at: 0 1249 1780 2674 3156 3904 4390 4579 5868 Size: 1249 531 894 482 748 486 189 1289 Fragments arranged by size: 1289 1249 894 748 531 486 482 189 Enzymes that do cut: AarI AcuI AflII AflIII AlfI AloI AlwNI ApaI ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NsiI NspI PasI PciI PflMI PfoI PpiI PpuMI PspOMI PspXI PstI PvuII SacI SapI SbfI ScaI SexAI SfcI SfiI SfoI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AgeI AhdI AleI ApaLI AscI AseI AsiSI BaeI BcgI BplI BsiWI BssHII BstZ17I ClaI FseI FspAI HpaI KpnI MfeI MluI NotI NruI PacI PmeI PmlI PshAI PsiI PsrI PvuI RsrII SacII SalI SanDI SgfI SgrAI SnaBI SrfI SwaI ZraI