Restriction map for
pFN21AE3039
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BamHI BbeI BbsI BciVI BclI
BfaI BfrI BlnI BlpI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrGI
Bst1107I BstBI BstF5I BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoRI EcoRV EcoT22I FbaI
FokI FseI FspI HgaI Hin1I
HincII HindII HindIII HinfI HpaI
KasI KspI MaeI MfeI MluI
MroI MunI NarI NcoI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmeI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tsp509I
TspRI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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Acc65I 355 GGTACC G^GTACC
AfaI 356 GTAC GT^AC
AlwI 261 GATCC GGATC(4/5)
Asp718I 355 GGTACC G^GTACC
AvaI 58 CCCGGG C^[CT]CG[GA]G
BalI 446 TGGCCA TGG^CCA
BanI 355 GGTACC G^G[CT][GA]CC
BbrPI 30 CACGTG CAC^GTG
BbvCI 111 CCTCAGC CCTCAGC(-5/-2)
BcgI 44 GCAGCGCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 64 GCCGGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 488 AGATCT A^GATCT
BsaAI 30 CACGTG [CT]AC^GT[GA]
BsaBI 255 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 58 CCCGGG C^[CT]CG[GA]G
BsrBI 411 GAGCGG CCGCTC(-3/-3)
BsrFI 64 GCCGGC [GA]^CCGG[CT]
BssHII 47 GCGCGC G^CGCGC
BssSI 492 CTCGTG CACGAG(-5/-1)
BstAPI 26 GCACCACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 276 CCTGG CC^[AT]GG
BstYI 488 AGATCT [GA]^GATC[CT]
Cfr10I 64 GCCGGC [GA]^CCGG[CT]
DdeI 112 CTCAG C^T[ACGT]AG
DraIII 27 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 446 TGGCCA [CT]^GGCC[GA]
EarI 286 CTCTTC CTCTTC(1/4)
EcoRII 276 CCTGG ^CC[AT]GG
EcoT14I 92 CCTTGG C^C[AT][AT]GG
FauI 122 CCCGC CCCGC(4/6)
HaeII 214 AGCGCC [GA]GCGC^[CT]
KpnI 355 GGTACC GGTAC^C
Ksp632I 286 CTCTTC CTCTTC(1/4)
MaeII 31 ACGT A^CGT
MamI 255 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 287 TCTTC GAAGA(8/7)
MflI 488 AGATCT [GA]^GATC[CT]
MluNI 446 TGGCCA TGG^CCA
MscI 446 TGGCCA TGG^CCA
MseI 349 TTAA T^TAA
MslI 180 CATCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 276 CCTGG CC^[AT]GG
NaeI 64 GCCGGC GCC^GGC
NdeI 471 CATATG CA^TATG
NgoMIV 64 GCCGGC G^CCGGC
PmaCI 30 CACGTG CAC^GTG
PmlI 30 CACGTG CAC^GTG
PvuII 318 CAGCTG CAG^CTG
RsaI 356 GTAC GT^AC
SacI 192 GAGCTC GAGCT^C
SexAI 275 ACCTGGT A^CC[AT]GGT
SfaNI 514 GATGC GCATC(5/9)
SfiI 63 GGCCGGCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 58 CCCGGG CCC^GGG
StyI 92 CCTTGG C^C[AT][AT]GG
Tru9I 349 TTAA T^TAA
XhoII 488 AGATCT [GA]^GATC[CT]
XmaCI 58 CCCGGG C^CCGGG
XmaI 58 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AspHI 192 GAGCTC G[AT]GC[AT]^C
304 GTGCTC
BseRI 364 CTCCTC GAGGAG(10/8)
367 CTCCTC
BsgI 340 CTGCAC GTGCAG(16/14)
33 GTGCAG
BsiHKAI 192 GAGCTC G[AT]GC[AT]^C
304 GTGCTC
BsrI 417 ACTGG ACTGG(1/-1)
504 CCAGT
BstEII 159 GGTCACC G^GT[ACGT]ACC
237 GGTCACC
BstPI 159 GGTCACC G^GT[ACGT]ACC
237 GGTCACC
EcoO65I 159 GGTCACC G^GT[ACGT]ACC
237 GGTCACC
MspA1I 39 CAGCGG C[AC]G^C[GT]G
318 CAGCTG
NlaIII 444 CATG CATG^
462 CATG
SfcI 210 CTACAG C^T[GA][CT]AG
219 CTACAG
Tsp45I 160 GTCAC ^GT[GC]AC
238 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AccII AciI AluI AvaII BanII
BbvI BcnI BmyI BsaJI BsiYI
BslI Bsp1286I BssKI BstUI Cac8I
CfoI Cfr13I DpnII Fnu4HI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeIII MboI MnlI MspI
MvnI MwoI NciI NdeII NlaIV
Sau3AI Sau96I ScrFI TseI