Restriction map for
pFN21AE3039
(Linear) (Six-base) MAPSORT of: KIEE3039.seq Check: 8849 from: 1 to: 525
KIEE3039.orf
With 182 enzymes: SgfI *
March 22, 2012 16:05 ..
Acc65I G'GTAC_C
Cuts at: 0 355 525
Size: 355 170
AvaI C'yCGr_G
Cuts at: 0 58 525
Size: 58 467
BanI G'GyrC_C
Cuts at: 0 355 525
Size: 355 170
BanII G_rGCy'C
Cuts at: 0 20 91 196 525
Size: 20 71 105 329
Fragments arranged by size:
329 105 71 20
BbvCI CC'TCA_GC
Cuts at: 0 112 525
Size: 112 413
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 33 67 525
Size: 33 34 458
Fragments arranged by size:
458 34 33
BglI GCCn_nnn'nGGC
Cuts at: 0 70 525
Size: 70 455
BglII A'GATC_T
Cuts at: 0 488 525
Size: 488 37
BmrI ACTGGGnnnn_n'
Cuts at: 0 498 525
Size: 498 27
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 34 525
Size: 34 491
Bpu10I CC'TnA_GC
Cuts at: 0 112 525
Size: 112 413
BsaAI yAC'GTr
Cuts at: 0 32 525
Size: 32 493
BsaBI GATnn'nnATC
Cuts at: 0 259 525
Size: 259 266
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 355 358 525
Size: 355 3 167
Fragments arranged by size:
355 167 3
BseYI C'CCAG_C
Cuts at: 0 74 79 525
Size: 74 5 446
Fragments arranged by size:
446 74 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 325 525
Size: 54 271 200
Fragments arranged by size:
271 200 54
BsiHKAI G_wGCw'C
Cuts at: 0 196 308 525
Size: 196 112 217
Fragments arranged by size:
217 196 112
Bsp1286I G_dGCh'C
Cuts at: 0 20 91 196 308 525
Size: 20 71 105 112 217
Fragments arranged by size:
217 112 105 71 20
BsrBI CCG'CTC
Cuts at: 0 413 525
Size: 413 112
BsrFI r'CCGG_y
Cuts at: 0 64 525
Size: 64 461
BssHII G'CGCG_C
Cuts at: 0 47 525
Size: 47 478
BssSI C'ACGA_G
Cuts at: 0 492 525
Size: 492 33
BstAPI GCAn_nnn'nTGC
Cuts at: 0 32 525
Size: 32 493
BstEII G'GTnAC_C
Cuts at: 0 159 237 525
Size: 159 78 288
Fragments arranged by size:
288 159 78
BstYI r'GATC_y
Cuts at: 0 488 525
Size: 488 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 357 525
Size: 357 168
DraIII CAC_nnn'GTG
Cuts at: 0 32 525
Size: 32 493
EaeI y'GGCC_r
Cuts at: 0 446 525
Size: 446 79
EarI CTCTTCn'nnn_
Cuts at: 0 292 525
Size: 292 233
EcoICRI GAG'CTC
Cuts at: 0 194 525
Size: 194 331
HaeII r_GCGC'y
Cuts at: 0 218 525
Size: 218 307
KpnI G_GTAC'C
Cuts at: 0 359 525
Size: 359 166
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 525
Size: 311 214
MscI TGG'CCA
Cuts at: 0 448 525
Size: 448 77
MslI CAynn'nnrTG
Cuts at: 0 184 525
Size: 184 341
MspA1I CmG'CkG
Cuts at: 0 41 320 525
Size: 41 279 205
Fragments arranged by size:
279 205 41
NaeI GCC'GGC
Cuts at: 0 66 525
Size: 66 459
NdeI CA'TA_TG
Cuts at: 0 472 525
Size: 472 53
NgoMIV G'CCGG_C
Cuts at: 0 64 525
Size: 64 461
PmlI CAC'GTG
Cuts at: 0 32 525
Size: 32 493
PvuII CAG'CTG
Cuts at: 0 320 525
Size: 320 205
SacI G_AGCT'C
Cuts at: 0 196 525
Size: 196 329
SexAI A'CCwGG_T
Cuts at: 0 275 525
Size: 275 250
SfcI C'TryA_G
Cuts at: 0 210 219 525
Size: 210 9 306
Fragments arranged by size:
306 210 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 70 525
Size: 70 455
SmaI CCC'GGG
Cuts at: 0 60 525
Size: 60 465
StyI C'CwwG_G
Cuts at: 0 92 525
Size: 92 433
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 142 525
Size: 142 383
XmaI C'CCGG_G
Cuts at: 0 58 525
Size: 58 467
Enzymes that do cut:
Acc65I AvaI BanI BanII BbvCI BcgI BglI BglII
BmrI BplI Bpu10I BsaAI BsaBI BseRI BseYI BsgI
BsiHKAI Bsp1286I BsrBI BsrFI BssHII BssSI BstAPI BstEII
BstYI BtgZI DraIII EaeI EarI EcoICRI HaeII KpnI
MmeI MscI MslI MspA1I NaeI NdeI NgoMIV PmlI
PvuII SacI SexAI SfcI SfiI SmaI StyI TaqII
XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbsI BciVI BclI BfrBI BlpI Bme1580I BmgBI BmtI
BpmI BpuEI BsaI BsaHI BsaWI BsaXI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI BstBI
BstXI BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraI
DrdI EagI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KasI MfeI MluI NarI NcoI NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI TstI Tth111I XbaI XcmI
XhoI XmnI ZraI