################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraIII DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI HinP1I HindIII HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MroI MseI MunI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SspBI SspI StuI SwaI Tru9I Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccI 395 GTAGAC GT^[AC][GT]AC AccII 941 CGCG CG^CG AcsI 29 GAATTT [GA]^AATT[CT] AcyI 162 GACGCC G[GA]^CG[CT]C AlwNI 446 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 29 GAATTT [GA]^AATT[CT] AvaI 853 CCCGGG C^[CT]CG[GA]G BanI 1117 GGCACC G^G[CT][GA]CC BbsI 692 GAAGAC GAAGAC(2/6) BfaI 292 CTAG C^TAG BglI 746 GCCCACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1030 AGATCT A^GATCT BpuAI 692 GAAGAC GAAGAC(2/6) BsaHI 162 GACGCC G[GA]^CG[CT]C BsoBI 853 CCCGGG C^[CT]CG[GA]G BsrFI 1226 ACCGGC [GA]^CCGG[CT] BstUI 941 CGCG CG^CG Cfr10I 1226 ACCGGC [GA]^CCGG[CT] DrdI 261 GACCTCGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FauI 939 CCCGC CCCGC(4/6) HgaI 162 GACGC GACGC(5/10) Hin1I 162 GACGCC G[GA]^CG[CT]C MaeI 292 CTAG C^TAG MvnI 941 CGCG CG^CG NdeI 1301 CATATG CA^TATG PflMI 434 CCATCGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1339 GACTC GAGTC(4/5) PshAI 577 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 1072 CAGCTG CAG^CTG SbfI 808 CCTGCAGG CCTGCA^GG ScaI 1325 AGTACT AGT^ACT SfaNI 1190 GCATC GCATC(5/9) SmaI 853 CCCGGG CCC^GGG SmlI 982 CTTGAG C^T[CT][GA]AG Sse8387I 808 CCTGCAGG CCTGCA^GG TaqI 1337 TCGA T^CGA Tsp509I 30 AATT ^AATT TthHB8I 1337 TCGA T^CGA Van91I 434 CCATCGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 853 CCCGGG C^CCGGG XmaI 853 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AfaI 1038 GTAC GT^AC 1326 GTAC BalI 138 TGGCCA TGG^CCA 442 TGGCCA BamHI 538 GGATCC G^GATCC 556 GGATCC BpmI 860 CTCCAG CTGGAG(16/14) 256 CTGGAG BspMI 807 ACCTGC ACCTGC(4/8) 1310 ACCTGC BsrDI 1008 CATTGC GCAATG(2/0) 1314 GCAATG DdeI 65 CTCAG C^T[ACGT]AG 99 CTCAG EcoT14I 524 CCAAGG C^C[AT][AT]GG 1053 CCTTGG HincII 773 GTCAAC GT[CT]^[GA]AC 964 GTCAAC HindII 773 GTCAAC GT[CT]^[GA]AC 964 GTCAAC MluNI 138 TGGCCA TGG^CCA 442 TGGCCA MscI 138 TGGCCA TGG^CCA 442 TGGCCA MslI 1225 CACCGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1231 CATGAGGGTG MspA1I 1072 CAGCTG C[AC]G^C[GT]G 1194 CCGCTG RsaI 1038 GTAC GT^AC 1326 GTAC StyI 524 CCAAGG C^C[AT][AT]GG 1053 CCTTGG TspRI 179 CACTG CA[GC]TG[ACGT][ACGT]^ 1101 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AciI AluI AlwI ApaI AvaII BanII BbvI BcnI BmyI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstEII BstF5I BstNI BstPI BstYI Cac8I Cfr13I DpnII DraII EaeI EcoO109I EcoO65I EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboI MboII MflI MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PpuMI PstI Sau3AI Sau96I ScrFI SfcI SphI TfiI TseI Tsp45I XhoII