(Circular) (Six-base) MAPSORT of: pFN21AE2437.seq Check: 1007 from: 1 to: 6070 pFN21AE2437 6070 bp With 182 enzymes: SgfI * December 4, 2012 13:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2821 2821 Size: 6070 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3880 278 Size: 53 83 186 936 2344 2468 Fragments arranged by size: 2468 2344 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2401 3413 1937 Size: 464 1012 4594 Fragments arranged by size: 4594 1012 464 Acc65I G'GTAC_C Cuts at: 3391 3391 Size: 6070 AclI AA'CG_TT Cuts at: 1563 4197 4570 1563 Size: 2634 373 3063 Fragments arranged by size: 3063 2634 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2027 2188 2478 4133 5517 5785 1632 Size: 395 161 290 1655 1384 268 1917 Fragments arranged by size: 1917 1655 1384 395 290 268 161 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5873 AflIII A'CryG_T Cuts at: 1203 4969 5946 1203 Size: 3766 977 1327 Fragments arranged by size: 3766 1327 977 AgeI A'CCGG_T Cuts at: 4901 4901 Size: 6070 AhdI GACnn_n'nnGTC Cuts at: 4799 4799 Size: 6070 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6070 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6070 AlwNI CAG_nnn'CTG Cuts at: 2456 3813 5385 2456 Size: 1357 1572 3141 Fragments arranged by size: 3141 1572 1357 ApaI G_GGCC'C Cuts at: 2439 2753 2799 2937 2439 Size: 314 46 138 5572 Fragments arranged by size: 5572 314 138 46 ApaLI G'TGCA_C Cuts at: 4127 5283 4127 Size: 1156 4914 ApoI r'AATT_y Cuts at: 1493 2034 3379 3605 3757 5866 1493 Size: 541 1345 226 152 2109 1697 Fragments arranged by size: 2109 1697 1345 541 226 152 AseI AT'TA_AT Cuts at: 160 1032 4624 160 Size: 872 3592 1606 Fragments arranged by size: 3592 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6070 AvaI C'yCGr_G Cuts at: 1870 1943 2858 3395 1870 Size: 73 915 537 4545 Fragments arranged by size: 4545 915 537 73 BamHI G'GATC_C Cuts at: 2543 2561 3400 3767 2543 Size: 18 839 367 4846 Fragments arranged by size: 4846 839 367 18 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3122 3391 4846 618 Size: 325 217 627 1335 269 1455 1842 Fragments arranged by size: 1842 1455 1335 627 325 269 217 BanII G_rGCy'C Cuts at: 1391 2439 2528 2753 2799 2937 3141 3389 1391 Size: 1048 89 225 46 138 204 248 4072 Fragments arranged by size: 4072 1048 248 225 204 138 89 46 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2704 928 Size: 971 805 4294 Fragments arranged by size: 4294 971 805 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4261 4295 1903 Size: 34 2324 34 3678 Fragments arranged by size: 3678 2324 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3962 5172 3962 Size: 1210 4860 BclI T'GATC_A Cuts at: 1548 1548 Size: 6070 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2757 4681 136 Size: 107 122 71 631 605 1085 1924 1525 Fragments arranged by size: 1924 1525 1085 631 605 122 107 71 BglII A'GATC_T Cuts at: 3035 6065 3035 Size: 3030 3040 BlpI GC'TnA_GC Cuts at: 3487 3487 Size: 6070 Bme1580I G_kGCm'C Cuts at: 1790 2439 2753 2799 2937 4131 5287 1790 Size: 649 314 46 138 1194 1156 2573 Fragments arranged by size: 2573 1194 1156 649 314 138 46 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6070 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2931 3322 3801 Size: 416 53 83 399 24 1514 391 479 Cuts at: 3801 4759 442 Size: 958 1753 Fragments arranged by size: 1753 1514 958 479 416 399 391 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6070 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2282 2850 4730 1239 Size: 1043 568 1880 2579 Fragments arranged by size: 2579 1880 1043 568 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3008 4195 5060 5358 3008 Size: 1187 865 298 3720 Fragments arranged by size: 3720 1187 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2258 3021 3281 3306 4733 882 Size: 617 759 763 260 25 1427 2219 Fragments arranged by size: 2219 1427 763 759 617 260 25 BsaAI yAC'GTr Cuts at: 493 1204 3861 493 Size: 711 2657 2702 Fragments arranged by size: 2702 2657 711 BsaBI GATnn'nnATC Cuts at: 6064 6064 Size: 6070 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2168 3877 4259 4894 Size: 53 83 186 936 635 1709 382 635 Cuts at: 4894 275 Size: 1451 Fragments arranged by size: 1709 1451 936 635 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4503 4901 5175 5322 5733 4503 Size: 398 274 147 411 4840 Fragments arranged by size: 4840 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2158 2188 3329 3359 1178 Size: 30 165 30 755 30 1141 30 3889 Fragments arranged by size: 3889 1141 755 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2163 2996 3170 1186 Size: 977 833 174 4086 Fragments arranged by size: 4086 977 833 174 BseYI C'CCAG_C Cuts at: 2117 5273 5769 2117 Size: 3156 496 2418 Fragments arranged by size: 3156 2418 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5497 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3520 4281 5309 1540 Size: 275 186 1519 761 1028 2301 Fragments arranged by size: 2301 1519 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4131 4216 5287 4131 Size: 85 1071 4914 Fragments arranged by size: 4914 1071 85 BsmI GAATG_Cn' Cuts at: 3581 3674 3581 Size: 93 5977 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2439 2528 2753 2799 2937 3141 3389 Size: 399 649 89 225 46 138 204 248 Cuts at: 3389 4131 4216 5287 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 649 399 248 225 204 138 89 85 46 BspEI T'CCGG_A Cuts at: 5733 5733 Size: 6070 BspHI T'CATG_A Cuts at: 3959 5689 3959 Size: 1730 4340 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2821 3324 3425 844 Size: 1977 503 101 3489 Fragments arranged by size: 3489 1977 503 101 BsrBI CCG'CTC Cuts at: 1394 1823 3957 1394 Size: 429 2134 3507 Fragments arranged by size: 3507 2134 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3012 3326 4565 4739 65 Size: 1183 1764 314 1239 174 1396 Fragments arranged by size: 1764 1396 1239 1183 314 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3231 4714 4901 1700 Size: 110 1421 1483 187 2869 Fragments arranged by size: 2869 1483 1421 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6070 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6070 BssSI C'ACGA_G Cuts at: 4130 5142 4130 Size: 1012 5058 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6070 BstBI TT'CG_AA Cuts at: 4874 4874 Size: 6070 BstEII G'GTnAC_C Cuts at: 1184 2480 2579 3227 1184 Size: 1296 99 648 4027 Fragments arranged by size: 4027 1296 648 99 BstYI r'GATC_y Cuts at: 1972 2543 2561 3035 3400 3767 4152 4169 5621 Size: 571 18 474 365 367 385 17 1452 Cuts at: 5621 5707 5719 6065 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 571 474 385 367 365 346 86 18 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4579 Fragments arranged by size: 4579 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3192 487 Size: 683 56 1966 3365 Fragments arranged by size: 3365 1966 683 56 BtsI GCAGTG_nn' Cuts at: 3105 3586 4380 4400 3105 Size: 481 794 20 4775 Fragments arranged by size: 4775 794 481 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6070 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6035 DraI TTT'AAA Cuts at: 3374 3374 Size: 6070 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2272 5077 809 Size: 507 956 2805 1802 Fragments arranged by size: 2805 1802 956 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2143 2212 2380 Size: 54 1425 50 275 18 313 69 168 Cuts at: 2380 2447 2785 3517 4406 6035 8 Size: 67 338 732 889 1629 43 Fragments arranged by size: 1629 1425 889 732 338 313 275 168 69 67 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3517 1537 Size: 275 1705 4090 Fragments arranged by size: 4090 1705 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4000 1353 Size: 2647 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4657 5031 5177 387 Size: 1064 138 3068 374 146 1280 Fragments arranged by size: 3068 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2027 2188 2282 2478 2850 4133 4730 Size: 393 395 161 94 196 372 1283 597 Cuts at: 4730 5517 5785 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1283 787 597 395 393 372 268 196 161 94 EcoO109I rG'GnC_Cy Cuts at: 2453 2462 2588 2687 2795 2796 2804 2834 2453 Size: 9 126 99 108 1 8 30 5689 Fragments arranged by size: 5689 126 108 99 30 9 8 1 EcoRI G'AATT_C Cuts at: 3379 3757 3379 Size: 378 5692 FspI TGC'GCA Cuts at: 4576 4576 Size: 6070 HaeII r_GCGC'y Cuts at: 1678 5217 1678 Size: 3539 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2158 2190 3020 3052 4711 Size: 32 135 32 586 32 830 32 1659 Cuts at: 4711 4743 4785 4817 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1659 830 586 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2780 2971 3414 3660 669 Size: 988 281 842 191 443 246 3079 Fragments arranged by size: 3079 988 842 443 281 246 191 HindIII A'AGCT_T Cuts at: 748 748 Size: 6070 HpaI GTT'AAC Cuts at: 3660 3660 Size: 6070 KpnI G_GTAC'C Cuts at: 3395 3395 Size: 6070 MfeI C'AATT_G Cuts at: 3669 3669 Size: 6070 MluI A'CGCG_T Cuts at: 5946 5946 Size: 6070 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5184 5368 1305 Size: 3879 184 2007 Fragments arranged by size: 3879 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2145 2449 10 Size: 54 1425 343 313 304 3631 Fragments arranged by size: 3631 1425 343 313 304 54 MslI CAynn'nnrTG Cuts at: 518 1166 3234 3240 4028 4387 4546 518 Size: 648 2068 6 788 359 159 2042 Fragments arranged by size: 2068 2042 788 648 359 159 6 MspA1I CmG'CkG Cuts at: 1606 3079 3201 3486 4163 5311 5556 1606 Size: 1473 122 285 677 1148 245 2120 Fragments arranged by size: 2120 1473 1148 677 285 245 122 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4579 Fragments arranged by size: 4579 939 552 NdeI CA'TA_TG Cuts at: 387 3307 387 Size: 2920 3150 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6070 NotI GC'GGCC_GC Cuts at: 3517 3517 Size: 6070 NspI r_CATG'y Cuts at: 1132 1229 2157 2287 2431 3285 3428 4973 1132 Size: 97 928 130 144 854 143 1545 2229 Fragments arranged by size: 2229 1545 928 854 144 143 130 97 PasI CC'CwG_GG Cuts at: 1348 2306 2323 2466 3299 1348 Size: 958 17 143 833 4119 Fragments arranged by size: 4119 958 833 143 17 PciI A'CATG_T Cuts at: 4969 4969 Size: 6070 PflMI CCAn_nnn'nTGG Cuts at: 1108 2445 1108 Size: 1337 4733 PfoI T'CCnGG_A Cuts at: 2620 2866 2620 Size: 246 5824 PmeI GTTT'AAAC Cuts at: 3374 3374 Size: 6070 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4139 4171 5652 5684 4139 Size: 32 1481 32 4525 Fragments arranged by size: 4525 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2462 2588 2687 2834 2462 Size: 126 99 147 5698 Fragments arranged by size: 5698 147 126 99 PshAI GACnn'nnGTC Cuts at: 1613 2586 1613 Size: 973 5097 PsiI TTA'TAA Cuts at: 2828 3640 2828 Size: 812 5258 PspOMI G'GGCC_C Cuts at: 2435 2749 2795 2933 2435 Size: 314 46 138 5572 Fragments arranged by size: 5572 314 138 46 PstI C_TGCA'G Cuts at: 830 2818 3112 3147 3422 830 Size: 1988 294 35 275 3478 Fragments arranged by size: 3478 1988 294 275 35 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6070 PvuII CAG'CTG Cuts at: 3079 3079 Size: 6070 SalI G'TCGA_C Cuts at: 1936 3412 1936 Size: 1476 4594 SanDI GG'GwC_CC Cuts at: 2588 2834 2588 Size: 246 5824 SbfI CC_TGCA'GG Cuts at: 2818 3422 2818 Size: 604 5466 ScaI AGT'ACT Cuts at: 3332 4318 6030 3332 Size: 986 1712 3372 Fragments arranged by size: 3372 1712 986 SfcI C'TryA_G Cuts at: 826 1045 2814 3108 3143 3418 4553 5234 5425 Size: 219 1769 294 35 275 1135 681 191 Cuts at: 5425 826 Size: 1471 Fragments arranged by size: 1769 1471 1135 681 294 275 219 191 35 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6070 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6070 SmaI CCC'GGG Cuts at: 1872 2860 3397 1872 Size: 988 537 4545 Fragments arranged by size: 4545 988 537 SmlI C'TyrA_G Cuts at: 820 1017 1943 2987 4174 5075 5337 820 Size: 197 926 1044 1187 901 262 1553 Fragments arranged by size: 1553 1187 1044 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3861 493 Size: 3368 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6070 SphI G_CATG'C Cuts at: 1132 2157 2287 2431 3428 1132 Size: 1025 130 144 997 3774 Fragments arranged by size: 3774 1025 997 144 130 SspI AAT'ATT Cuts at: 5 52 3994 5 Size: 47 3942 2081 Fragments arranged by size: 3942 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6070 StyI C'CwwG_G Cuts at: 513 1065 2004 2529 3058 3509 513 Size: 552 939 525 529 451 3074 Fragments arranged by size: 3074 939 552 529 525 451 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3017 3173 3341 4097 4114 4267 4452 Size: 1255 1437 156 168 756 17 153 185 Cuts at: 4452 4897 325 Size: 445 1498 Fragments arranged by size: 1498 1437 1255 756 445 185 168 156 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3042 3330 4316 Size: 274 80 33 51 1443 1065 288 986 Cuts at: 4316 6028 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1065 986 288 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2172 2204 6030 6062 2172 Size: 32 3826 32 2180 Fragments arranged by size: 3826 2180 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6070 XbaI T'CTAG_A Cuts at: 3406 3406 Size: 6070 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6070 XmaI C'CCGG_G Cuts at: 1870 2858 3395 1870 Size: 988 537 4545 Fragments arranged by size: 4545 988 537 XmnI GAAnn'nnTTC Cuts at: 4199 4199 Size: 6070 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3878 276 Size: 53 83 186 936 2344 2468 Fragments arranged by size: 2468 2344 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI