(Linear) (Six-base) MAPSORT of: KIEE2437.seq Check: 2989 from: 1 to: 1365 KIEE2437.orf With 182 enzymes: SgfI * December 4, 2012 11:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 816 1365 Size: 816 549 AccI GT'mk_AC Cuts at: 0 396 1365 Size: 396 969 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 22 183 473 1365 Size: 22 161 290 892 Fragments arranged by size: 892 290 161 22 AlwNI CAG_nnn'CTG Cuts at: 0 451 1365 Size: 451 914 ApaI G_GGCC'C Cuts at: 0 434 748 794 932 1365 Size: 434 314 46 138 433 Fragments arranged by size: 434 433 314 138 46 ApoI r'AATT_y Cuts at: 0 29 1365 Size: 29 1336 AvaI C'yCGr_G Cuts at: 0 853 1365 Size: 853 512 BamHI G'GATC_C Cuts at: 0 538 556 1365 Size: 538 18 809 Fragments arranged by size: 809 538 18 BanI G'GyrC_C Cuts at: 0 1117 1365 Size: 1117 248 BanII G_rGCy'C Cuts at: 0 434 523 748 794 932 1136 1365 Size: 434 89 225 46 138 204 229 Fragments arranged by size: 434 229 225 204 138 89 46 BbsI GAAGACnn'nnnn_ Cuts at: 0 699 1365 Size: 699 666 BglI GCCn_nnn'nGGC Cuts at: 0 752 1365 Size: 752 613 BglII A'GATC_T Cuts at: 0 1030 1365 Size: 1030 335 Bme1580I G_kGCm'C Cuts at: 0 434 748 794 932 1365 Size: 434 314 46 138 433 Fragments arranged by size: 434 433 314 138 46 BmrI ACTGGGnnnn_n' Cuts at: 0 926 1317 1365 Size: 926 391 48 Fragments arranged by size: 926 391 48 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 277 845 1365 Size: 277 568 520 Fragments arranged by size: 568 520 277 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1003 1365 Size: 1003 362 BsaI GGTCTCn'nnnn_ Cuts at: 0 253 1016 1276 1301 1365 Size: 253 763 260 25 64 Fragments arranged by size: 763 260 253 64 25 BsaHI Gr'CG_yC Cuts at: 0 163 1365 Size: 163 1202 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 153 183 1324 1354 1365 Size: 153 30 1141 30 11 Fragments arranged by size: 1141 153 30 30 11 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 158 991 1165 1365 Size: 158 833 174 200 Fragments arranged by size: 833 200 174 158 BseYI C'CCAG_C Cuts at: 0 112 1365 Size: 112 1253 Bsp1286I G_dGCh'C Cuts at: 0 434 523 748 794 932 1136 1365 Size: 434 89 225 46 138 204 229 Fragments arranged by size: 434 229 225 204 138 89 46 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 816 1319 1365 Size: 816 503 46 Fragments arranged by size: 816 503 46 BsrDI GCAATG_nn' Cuts at: 0 1007 1321 1365 Size: 1007 314 44 Fragments arranged by size: 1007 314 44 BsrFI r'CCGG_y Cuts at: 0 1226 1365 Size: 1226 139 BstEII G'GTnAC_C Cuts at: 0 475 574 1222 1365 Size: 475 99 648 143 Fragments arranged by size: 648 475 143 99 BstYI r'GATC_y Cuts at: 0 538 556 1030 1365 Size: 538 18 474 335 Fragments arranged by size: 538 474 335 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1187 1365 Size: 1187 178 BtsI GCAGTG_nn' Cuts at: 0 1100 1365 Size: 1100 265 DrdI GACnn_nn'nnGTC Cuts at: 0 267 1365 Size: 267 1098 EaeI y'GGCC_r Cuts at: 0 138 207 375 442 780 1365 Size: 138 69 168 67 338 585 Fragments arranged by size: 585 338 168 138 69 67 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 22 183 277 473 845 1365 Size: 22 161 94 196 372 520 Fragments arranged by size: 520 372 196 161 94 22 EcoO109I rG'GnC_Cy Cuts at: 0 448 457 583 682 790 791 799 829 Size: 448 9 126 99 108 1 8 30 Cuts at: 829 1365 Size: 536 Fragments arranged by size: 536 448 126 108 99 30 9 8 1 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 153 185 1015 1047 1365 Size: 153 32 830 32 318 Fragments arranged by size: 830 318 153 32 32 HincII GTy'rAC Cuts at: 0 775 966 1365 Size: 775 191 399 Fragments arranged by size: 775 399 191 MscI TGG'CCA Cuts at: 0 140 444 1365 Size: 140 304 921 Fragments arranged by size: 921 304 140 MslI CAynn'nnrTG Cuts at: 0 1229 1235 1365 Size: 1229 6 130 Fragments arranged by size: 1229 130 6 MspA1I CmG'CkG Cuts at: 0 1074 1196 1365 Size: 1074 122 169 Fragments arranged by size: 1074 169 122 NdeI CA'TA_TG Cuts at: 0 1302 1365 Size: 1302 63 NspI r_CATG'y Cuts at: 0 152 282 426 1280 1365 Size: 152 130 144 854 85 Fragments arranged by size: 854 152 144 130 85 PasI CC'CwG_GG Cuts at: 0 301 318 461 1294 1365 Size: 301 17 143 833 71 Fragments arranged by size: 833 301 143 71 17 PflMI CCAn_nnn'nTGG Cuts at: 0 440 1365 Size: 440 925 PfoI T'CCnGG_A Cuts at: 0 615 861 1365 Size: 615 246 504 Fragments arranged by size: 615 504 246 PpuMI rG'GwC_Cy Cuts at: 0 457 583 682 829 1365 Size: 457 126 99 147 536 Fragments arranged by size: 536 457 147 126 99 PshAI GACnn'nnGTC Cuts at: 0 581 1365 Size: 581 784 PsiI TTA'TAA Cuts at: 0 823 1365 Size: 823 542 PspOMI G'GGCC_C Cuts at: 0 430 744 790 928 1365 Size: 430 314 46 138 437 Fragments arranged by size: 437 430 314 138 46 PstI C_TGCA'G Cuts at: 0 813 1107 1142 1365 Size: 813 294 35 223 Fragments arranged by size: 813 294 223 35 PvuII CAG'CTG Cuts at: 0 1074 1365 Size: 1074 291 SanDI GG'GwC_CC Cuts at: 0 583 829 1365 Size: 583 246 536 Fragments arranged by size: 583 536 246 SbfI CC_TGCA'GG Cuts at: 0 813 1365 Size: 813 552 ScaI AGT'ACT Cuts at: 0 1327 1365 Size: 1327 38 SfcI C'TryA_G Cuts at: 0 809 1103 1138 1365 Size: 809 294 35 227 Fragments arranged by size: 809 294 227 35 SmaI CCC'GGG Cuts at: 0 855 1365 Size: 855 510 SmlI C'TyrA_G Cuts at: 0 982 1365 Size: 982 383 SphI G_CATG'C Cuts at: 0 152 282 426 1365 Size: 152 130 144 939 Fragments arranged by size: 939 152 144 130 StyI C'CwwG_G Cuts at: 0 524 1053 1365 Size: 524 529 312 Fragments arranged by size: 529 524 312 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1012 1168 1336 1365 Size: 1012 156 168 29 Fragments arranged by size: 1012 168 156 29 TatI w'GTAC_w Cuts at: 0 1037 1325 1365 Size: 1037 288 40 Fragments arranged by size: 1037 288 40 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 167 199 1365 Size: 167 32 1166 Fragments arranged by size: 1166 167 32 XmaI C'CCGG_G Cuts at: 0 853 1365 Size: 853 512 Enzymes that do cut: AarI AccI AcuI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbsI BglI BglII Bme1580I BmrI BpmI BpuEI BsaI BsaHI BsaXI BseRI BseYI Bsp1286I BspMI BsrDI BsrFI BstEII BstYI BtgZI BtsI DrdI EaeI Eco57MI EcoO109I Hin4I HincII MscI MslI MspA1I NdeI NspI PasI PflMI PfoI PpuMI PshAI PsiI PspOMI PstI PvuII SanDI SbfI ScaI SfcI SmaI SmlI SphI StyI TaqII TatI TstI XmaI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BlpI BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsaWI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraI DraIII EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PspXI PsrI PvuI RsrII SacI SacII SalI SapI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmnI ZraI