(Circular) (Six-base) MAPSORT of: pFN21AE1831.seq Check: 2244 from: 1 to: 5752 pFN21AE1831 5752 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2691 2704 3026 3053 2691 Size: 13 322 27 5390 Fragments arranged by size: 5390 322 27 13 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3562 278 Size: 53 83 186 936 2026 2468 Fragments arranged by size: 2468 2026 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3095 1937 Size: 1158 4594 Acc65I G'GTAC_C Cuts at: 3073 3073 Size: 5752 AclI AA'CG_TT Cuts at: 1563 3879 4252 1563 Size: 2316 373 3063 Fragments arranged by size: 3063 2316 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2708 3815 5199 5467 1632 Size: 1076 1107 1384 268 1917 Fragments arranged by size: 1917 1384 1107 1076 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5555 AflIII A'CryG_T Cuts at: 1203 2037 4651 5628 1203 Size: 834 2614 977 1327 Fragments arranged by size: 2614 1327 977 834 AgeI A'CCGG_T Cuts at: 4583 4583 Size: 5752 AhdI GACnn_n'nnGTC Cuts at: 4481 4481 Size: 5752 AleI CACnn'nnGTG Cuts at: 1166 2963 1166 Size: 1797 3955 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5752 AlwNI CAG_nnn'CTG Cuts at: 3495 5067 3495 Size: 1572 4180 ApaLI G'TGCA_C Cuts at: 3809 4965 3809 Size: 1156 4596 ApoI r'AATT_y Cuts at: 1493 2984 3061 3287 3439 5548 1493 Size: 1491 77 226 152 2109 1697 Fragments arranged by size: 2109 1697 1491 226 152 77 AseI AT'TA_AT Cuts at: 160 1032 4306 160 Size: 872 3274 1606 Fragments arranged by size: 3274 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5752 AvaI C'yCGr_G Cuts at: 1870 1943 2511 3077 1870 Size: 73 568 566 4545 Fragments arranged by size: 4545 568 566 73 BamHI G'GATC_C Cuts at: 3082 3449 3082 Size: 367 5385 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2535 3073 4528 618 Size: 325 217 627 748 538 1455 1842 Fragments arranged by size: 1842 1455 748 627 538 325 217 BanII G_rGCy'C Cuts at: 1391 2295 3071 1391 Size: 904 776 4072 Fragments arranged by size: 4072 904 776 BbeI G_GCGC'C Cuts at: 2539 2539 Size: 5752 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2272 2279 3031 928 Size: 971 373 7 752 3649 Fragments arranged by size: 3649 971 752 373 7 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3943 3977 1903 Size: 34 50 34 1922 34 3678 Fragments arranged by size: 3678 1922 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3644 4854 3644 Size: 1210 4542 BclI T'GATC_A Cuts at: 1548 1548 Size: 5752 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4363 136 Size: 107 122 71 631 605 2691 1525 Fragments arranged by size: 2691 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5747 5747 Size: 5752 BlpI GC'TnA_GC Cuts at: 3169 3169 Size: 5752 Bme1580I G_kGCm'C Cuts at: 1790 2870 3813 4969 1790 Size: 1080 943 1156 2573 Fragments arranged by size: 2573 1156 1080 943 BmgBI CAC'GTC Cuts at: 1318 2470 2895 1318 Size: 1152 425 4175 Fragments arranged by size: 4175 1152 425 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3483 4441 442 Size: 416 53 83 399 24 2066 958 1753 Fragments arranged by size: 2066 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5752 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2618 4412 1239 Size: 1379 1794 2579 Fragments arranged by size: 2579 1794 1379 Bpu10I CC'TnA_GC Cuts at: 2387 2387 Size: 5752 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3877 4742 5040 3877 Size: 865 298 4589 Fragments arranged by size: 4589 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4415 882 Size: 617 2916 2219 Fragments arranged by size: 2916 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2038 2065 2325 3543 493 Size: 711 834 27 260 1218 2702 Fragments arranged by size: 2702 1218 834 711 260 27 BsaBI GATnn'nnATC Cuts at: 2210 5746 2210 Size: 3536 2216 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2055 2536 3559 3941 Size: 53 83 186 936 522 481 1023 382 Cuts at: 3941 4576 275 Size: 635 1451 Fragments arranged by size: 1451 1023 936 635 522 481 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2586 4185 4583 4857 5004 5415 2586 Size: 1599 398 274 147 411 2923 Fragments arranged by size: 2923 1599 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2591 2621 1178 Size: 30 165 30 1188 30 4309 Fragments arranged by size: 4309 1188 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2855 1186 Size: 1669 4083 BseYI C'CCAG_C Cuts at: 4955 5451 4955 Size: 496 5256 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2614 2732 1163 Size: 573 878 118 4183 Fragments arranged by size: 4183 878 573 118 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2407 2466 2709 2935 3202 3963 Size: 275 186 406 59 243 226 267 761 Cuts at: 3963 4991 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 406 275 267 243 226 186 59 BsiHKAI G_wGCw'C Cuts at: 2080 2295 3813 3898 4969 2080 Size: 215 1518 85 1071 2863 Fragments arranged by size: 2863 1518 1071 215 85 BsiWI C'GTAC_G Cuts at: 2325 2325 Size: 5752 BsmI GAATG_Cn' Cuts at: 3263 3356 3263 Size: 93 5659 BsmBI CGTCTCn'nnnn_ Cuts at: 2885 2885 Size: 5752 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2080 2295 2870 3071 3813 3898 4969 Size: 399 290 215 575 201 742 85 1071 Cuts at: 4969 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 575 399 290 215 201 85 BspEI T'CCGG_A Cuts at: 5415 5415 Size: 5752 BspHI T'CATG_A Cuts at: 2541 3641 5371 2541 Size: 1100 1730 2922 Fragments arranged by size: 2922 1730 1100 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2556 2651 2691 2704 3026 3053 3107 844 Size: 1712 95 40 13 322 27 54 3489 Fragments arranged by size: 3489 1712 322 95 54 40 27 13 BsrBI CCG'CTC Cuts at: 1394 1823 3639 1394 Size: 429 1816 3507 Fragments arranged by size: 3507 1816 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4247 4421 65 Size: 1183 2999 174 1396 Fragments arranged by size: 2999 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2564 4396 4583 1700 Size: 110 754 1832 187 2869 Fragments arranged by size: 2869 1832 754 187 110 BsrGI T'GTAC_A Cuts at: 96 2811 96 Size: 2715 3037 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5752 BssSI C'ACGA_G Cuts at: 3812 4824 3812 Size: 1012 4740 BstAPI GCAn_nnn'nTGC Cuts at: 794 3018 794 Size: 2224 3528 BstBI TT'CG_AA Cuts at: 4556 4556 Size: 5752 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5752 BstYI r'GATC_y Cuts at: 1972 3082 3449 3834 3851 5303 5389 5401 5747 Size: 1110 367 385 17 1452 86 12 346 Cuts at: 5747 1972 Size: 1977 Fragments arranged by size: 1977 1452 1110 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2088 2141 2337 2611 2793 2853 513 Size: 552 1023 53 196 274 182 60 3412 Fragments arranged by size: 3412 1023 552 274 196 182 60 53 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5013 Fragments arranged by size: 5013 683 56 BtsI GCAGTG_nn' Cuts at: 2978 3268 4062 4082 2978 Size: 290 794 20 4648 Fragments arranged by size: 4648 794 290 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5752 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5717 DraI TTT'AAA Cuts at: 3056 3056 Size: 5752 DraIII CAC_nnn'GTG Cuts at: 2617 2925 2617 Size: 308 5444 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4759 809 Size: 507 3443 1802 Fragments arranged by size: 3443 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2463 2706 2850 Size: 54 1425 50 275 18 633 243 144 Cuts at: 2850 2932 3199 4088 5717 8 Size: 82 267 889 1629 43 Fragments arranged by size: 1629 1425 889 633 275 267 243 144 82 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2463 2706 2932 3199 1537 Size: 275 651 243 226 267 4090 Fragments arranged by size: 4090 651 275 267 243 226 EarI CTCTTCn'nnn_ Cuts at: 1353 3682 1353 Size: 2329 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4339 4713 4859 387 Size: 1064 138 2750 374 146 1280 Fragments arranged by size: 2750 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2293 2293 Size: 5752 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2618 2708 3815 4412 5199 5467 1239 Size: 393 986 90 1107 597 787 268 1524 Fragments arranged by size: 1524 1107 986 787 597 393 268 90 EcoNI CCTnn'n_nnAGG Cuts at: 2687 2687 Size: 5752 EcoRI G'AATT_C Cuts at: 3061 3439 3061 Size: 378 5374 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2296 2296 Size: 5752 FspI TGC'GCA Cuts at: 2762 2777 4258 2762 Size: 15 1481 4256 Fragments arranged by size: 4256 1481 15 HaeII r_GCGC'y Cuts at: 1678 2500 2539 4899 1678 Size: 822 39 2360 2531 Fragments arranged by size: 2531 2360 822 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2094 2126 4393 4425 4467 Size: 32 135 32 522 32 2267 32 42 Cuts at: 4467 4499 1373 Size: 32 2626 Fragments arranged by size: 2626 2267 522 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3096 3342 669 Size: 988 281 1158 246 3079 Fragments arranged by size: 3079 1158 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5752 HpaI GTT'AAC Cuts at: 3342 3342 Size: 5752 KasI G'GCGC_C Cuts at: 2535 2535 Size: 5752 KpnI G_GTAC'C Cuts at: 3077 3077 Size: 5752 MfeI C'AATT_G Cuts at: 3351 3351 Size: 5752 MluI A'CGCG_T Cuts at: 5628 5628 Size: 5752 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4866 5050 1305 Size: 3561 184 2007 Fragments arranged by size: 3561 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3930 Fragments arranged by size: 3930 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2183 2556 2963 3710 4069 4228 518 Size: 648 1017 373 407 747 359 159 2042 Fragments arranged by size: 2042 1017 747 648 407 373 359 159 MspA1I CmG'CkG Cuts at: 1606 2143 2339 2366 2596 2773 3168 3845 4993 Size: 537 196 27 230 177 395 677 1148 Cuts at: 4993 5238 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 537 395 245 230 196 177 27 NaeI GCC'GGC Cuts at: 2566 2566 Size: 5752 NarI GG'CG_CC Cuts at: 2536 2536 Size: 5752 NcoI C'CATG_G Cuts at: 513 1065 2793 513 Size: 552 1728 3472 Fragments arranged by size: 3472 1728 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5752 NgoMIV G'CCGG_C Cuts at: 2564 2564 Size: 5752 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5752 NotI GC'GGCC_GC Cuts at: 3199 3199 Size: 5752 NspI r_CATG'y Cuts at: 1132 1229 3110 4655 1132 Size: 97 1881 1545 2229 Fragments arranged by size: 2229 1881 1545 97 PasI CC'CwG_GG Cuts at: 1348 2098 1348 Size: 750 5002 PciI A'CATG_T Cuts at: 4651 4651 Size: 5752 PflMI CCAn_nnn'nTGG Cuts at: 1108 2967 1108 Size: 1859 3893 PmeI GTTT'AAAC Cuts at: 3056 3056 Size: 5752 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3821 3853 5334 5366 3821 Size: 32 1481 32 4207 Fragments arranged by size: 4207 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5752 PsiI TTA'TAA Cuts at: 3322 3322 Size: 5752 PstI C_TGCA'G Cuts at: 830 2375 3023 3104 830 Size: 1545 648 81 3478 Fragments arranged by size: 3478 1545 648 81 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5752 PvuII CAG'CTG Cuts at: 2596 2773 2596 Size: 177 5575 SacI G_AGCT'C Cuts at: 2295 2295 Size: 5752 SacII CC_GC'GG Cuts at: 2144 2340 2144 Size: 196 5556 SalI G'TCGA_C Cuts at: 1936 3094 1936 Size: 1158 4594 SbfI CC_TGCA'GG Cuts at: 3104 3104 Size: 5752 ScaI AGT'ACT Cuts at: 4000 5712 4000 Size: 1712 4040 SexAI A'CCwGG_T Cuts at: 2805 2805 Size: 5752 SfcI C'TryA_G Cuts at: 826 1045 2371 3019 3100 4235 4916 5107 826 Size: 219 1326 648 81 1135 681 191 1471 Fragments arranged by size: 1471 1326 1135 681 648 219 191 81 SfoI GGC'GCC Cuts at: 2537 2537 Size: 5752 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5752 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5752 SmaI CCC'GGG Cuts at: 1872 3079 1872 Size: 1207 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3856 4757 5019 820 Size: 197 926 1913 901 262 1553 Fragments arranged by size: 1913 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3543 493 Size: 3050 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5752 SphI G_CATG'C Cuts at: 1132 3110 1132 Size: 1978 3774 SspI AAT'ATT Cuts at: 5 52 3676 5 Size: 47 3624 2081 Fragments arranged by size: 3624 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5752 StyI C'CwwG_G Cuts at: 513 1065 2377 2793 2961 3191 513 Size: 552 1312 416 168 230 3074 Fragments arranged by size: 3074 1312 552 416 230 168 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2963 3779 3796 3949 4134 4579 325 Size: 1255 1383 816 17 153 185 445 1498 Fragments arranged by size: 1498 1383 1255 816 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2697 2811 2955 Size: 274 80 33 51 1443 720 114 144 Cuts at: 2955 3998 5710 96 Size: 1043 1712 138 Fragments arranged by size: 1712 1443 1043 720 274 144 138 114 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2591 2623 5712 5744 2591 Size: 32 3089 32 2599 Fragments arranged by size: 3089 2599 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5752 XbaI T'CTAG_A Cuts at: 2578 3088 2578 Size: 510 5242 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2618 2860 2618 Size: 242 5510 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5752 XmaI C'CCGG_G Cuts at: 1870 3077 1870 Size: 1207 4545 XmnI GAAnn'nnTTC Cuts at: 3881 3881 Size: 5752 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3560 276 Size: 53 83 186 936 2026 2468 Fragments arranged by size: 2468 2026 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BstXI BstZ17I Bsu36I EcoO109I EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SanDI SapI SfiI SrfI SwaI