(Linear) (Six-base) MAPSORT of: KIEE1831.seq Check: 1929 from: 1 to: 1047 KIEE1831.orf With 182 enzymes: SgfI * July 15, 2010 18:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 686 699 1021 1047 Size: 686 13 322 26 Fragments arranged by size: 686 322 26 13 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 703 1047 Size: 703 344 AflIII A'CryG_T Cuts at: 0 32 1047 Size: 32 1015 AleI CACnn'nnGTG Cuts at: 0 958 1047 Size: 958 89 ApoI r'AATT_y Cuts at: 0 979 1047 Size: 979 68 AvaI C'yCGr_G Cuts at: 0 506 1047 Size: 506 541 BanI G'GyrC_C Cuts at: 0 530 1047 Size: 530 517 BanII G_rGCy'C Cuts at: 0 290 1047 Size: 290 757 BbeI G_GCGC'C Cuts at: 0 534 1047 Size: 534 513 BbsI GAAGACnn'nnnn_ Cuts at: 0 267 274 1026 1047 Size: 267 7 752 21 Fragments arranged by size: 752 267 21 7 Bme1580I G_kGCm'C Cuts at: 0 865 1047 Size: 865 182 BmgBI CAC'GTC Cuts at: 0 465 890 1047 Size: 465 425 157 Fragments arranged by size: 465 425 157 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 613 1047 Size: 613 434 Bpu10I CC'TnA_GC Cuts at: 0 382 1047 Size: 382 665 BsaAI yAC'GTr Cuts at: 0 33 60 320 1047 Size: 33 27 260 727 Fragments arranged by size: 727 260 33 27 BsaBI GATnn'nnATC Cuts at: 0 205 1047 Size: 205 842 BsaHI Gr'CG_yC Cuts at: 0 50 531 1047 Size: 50 481 516 Fragments arranged by size: 516 481 50 BsaWI w'CCGG_w Cuts at: 0 581 1047 Size: 581 466 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 586 616 1047 Size: 586 30 431 Fragments arranged by size: 586 431 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 850 1047 Size: 850 197 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 609 727 1047 Size: 609 118 320 Fragments arranged by size: 609 320 118 BsiEI CG_ry'CG Cuts at: 0 402 461 704 930 1047 Size: 402 59 243 226 117 Fragments arranged by size: 402 243 226 117 59 BsiHKAI G_wGCw'C Cuts at: 0 75 290 1047 Size: 75 215 757 Fragments arranged by size: 757 215 75 BsiWI C'GTAC_G Cuts at: 0 320 1047 Size: 320 727 BsmBI CGTCTCn'nnnn_ Cuts at: 0 880 1047 Size: 880 167 Bsp1286I G_dGCh'C Cuts at: 0 75 290 865 1047 Size: 75 215 575 182 Fragments arranged by size: 575 215 182 75 BspHI T'CATG_A Cuts at: 0 536 1047 Size: 536 511 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 551 646 686 699 1021 1047 Size: 551 95 40 13 322 26 Fragments arranged by size: 551 322 95 40 26 13 BsrFI r'CCGG_y Cuts at: 0 559 1047 Size: 559 488 BsrGI T'GTAC_A Cuts at: 0 806 1047 Size: 806 241 BstAPI GCAn_nnn'nTGC Cuts at: 0 1013 1047 Size: 1013 34 BtgI C'CryG_G Cuts at: 0 83 136 332 606 788 848 1047 Size: 83 53 196 274 182 60 199 Fragments arranged by size: 274 199 196 182 83 60 53 BtsI GCAGTG_nn' Cuts at: 0 973 1047 Size: 973 74 DraIII CAC_nnn'GTG Cuts at: 0 612 920 1047 Size: 612 308 127 Fragments arranged by size: 612 308 127 EaeI y'GGCC_r Cuts at: 0 458 701 845 927 1047 Size: 458 243 144 82 120 Fragments arranged by size: 458 243 144 120 82 EagI C'GGCC_G Cuts at: 0 458 701 927 1047 Size: 458 243 226 120 Fragments arranged by size: 458 243 226 120 EcoICRI GAG'CTC Cuts at: 0 288 1047 Size: 288 759 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 613 703 1047 Size: 613 90 344 Fragments arranged by size: 613 344 90 EcoNI CCTnn'n_nnAGG Cuts at: 0 682 1047 Size: 682 365 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 291 1047 Size: 291 756 FspI TGC'GCA Cuts at: 0 757 772 1047 Size: 757 15 275 Fragments arranged by size: 757 275 15 HaeII r_GCGC'y Cuts at: 0 495 534 1047 Size: 495 39 513 Fragments arranged by size: 513 495 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 89 121 1047 Size: 89 32 926 Fragments arranged by size: 926 89 32 KasI G'GCGC_C Cuts at: 0 530 1047 Size: 530 517 MslI CAynn'nnrTG Cuts at: 0 178 551 958 1047 Size: 178 373 407 89 Fragments arranged by size: 407 373 178 89 MspA1I CmG'CkG Cuts at: 0 138 334 361 591 768 1047 Size: 138 196 27 230 177 279 Fragments arranged by size: 279 230 196 177 138 27 NaeI GCC'GGC Cuts at: 0 561 1047 Size: 561 486 NarI GG'CG_CC Cuts at: 0 531 1047 Size: 531 516 NcoI C'CATG_G Cuts at: 0 788 1047 Size: 788 259 NgoMIV G'CCGG_C Cuts at: 0 559 1047 Size: 559 488 PasI CC'CwG_GG Cuts at: 0 93 1047 Size: 93 954 PflMI CCAn_nnn'nTGG Cuts at: 0 962 1047 Size: 962 85 PstI C_TGCA'G Cuts at: 0 370 1018 1047 Size: 370 648 29 Fragments arranged by size: 648 370 29 PvuII CAG'CTG Cuts at: 0 591 768 1047 Size: 591 177 279 Fragments arranged by size: 591 279 177 SacI G_AGCT'C Cuts at: 0 290 1047 Size: 290 757 SacII CC_GC'GG Cuts at: 0 139 335 1047 Size: 139 196 712 Fragments arranged by size: 712 196 139 SexAI A'CCwGG_T Cuts at: 0 800 1047 Size: 800 247 SfcI C'TryA_G Cuts at: 0 366 1014 1047 Size: 366 648 33 Fragments arranged by size: 648 366 33 SfoI GGC'GCC Cuts at: 0 532 1047 Size: 532 515 StyI C'CwwG_G Cuts at: 0 372 788 956 1047 Size: 372 416 168 91 Fragments arranged by size: 416 372 168 91 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 958 1047 Size: 958 89 TatI w'GTAC_w Cuts at: 0 692 806 950 1047 Size: 692 114 144 97 Fragments arranged by size: 692 144 114 97 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 586 618 1047 Size: 586 32 429 Fragments arranged by size: 586 429 32 XbaI T'CTAG_A Cuts at: 0 573 1047 Size: 573 474 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 613 855 1047 Size: 613 242 192 Fragments arranged by size: 613 242 192 Enzymes that do cut: AarI AcuI AflIII AleI ApoI AvaI BanI BanII BbeI BbsI Bme1580I BmgBI BpmI Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspHI BspMI BsrFI BsrGI BstAPI BtgI BtsI DraIII EaeI EagI EcoICRI Eco57MI EcoNI FalI FspI HaeII Hin4I KasI MslI MspA1I NaeI NarI NcoI NgoMIV PasI PflMI PstI PvuII SacI SacII SexAI SfcI SfoI StyI TaqII TatI TstI XbaI XcmI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmrI BmtI BplI BpuEI BsaI BseYI BsmI BspEI BsrBI BsrDI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EarI EciI EcoO109I EcoRI EcoRV FseI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MscI NdeI NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XhoI XmaI XmnI ZraI