################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII AccIII AclI AcsI AcyI AflII AgeI AhdI AlwNI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BalI BanII BbeI BbvCI BcgI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaHI BsaI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI Bst1107I BstZ17I CelII ClaI CpoI DraI DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoRI EcoT22I FseI FspI HaeII Hin1I HpaI KasI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MunI NarI NdeI NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SexAI SfiI SfoI SgrAI SnaBI SpeI SphI SspBI SspI StuI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 370 GGTACC G^GTACC AccI 106 GTCTAC GT^[AC][GT]AC AccII 783 CGCG CG^CG AflIII 836 ACGTGT A^C[GA][CT]GT Alw44I 228 GTGCAC G^TGCAC AocI 365 CCTCAGG CC^T[ACGT]AGG ApaLI 228 GTGCAC G^TGCAC Asp718I 370 GGTACC G^GTACC BamHI 613 GGATCC G^GATCC BbrPI 835 CACGTG CAC^GTG BbsI 1173 GAAGAC GAAGAC(2/6) BclI 1256 TGATCA T^GATCA BpuAI 1173 GAAGAC GAAGAC(2/6) BsaAI 835 CACGTG [CT]AC^GT[GA] BsaWI 63 ACCGGA [AT]^CCGG[AT] BssHII 782 GCGCGC G^CGCGC BssSI 283 CTCGTG CACGAG(-5/-1) BstAPI 329 GCAGCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1341 TTCGAA TT^CGAA BstEII 17 GGTCACC G^GT[ACGT]ACC BstPI 17 GGTCACC G^GT[ACGT]ACC BstUI 783 CGCG CG^CG BstXI 913 CCATGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 613 GGATCC [GA]^GATC[CT] Bsu36I 365 CCTCAGG CC^T[ACGT]AGG EaeI 299 TGGCCG [CT]^GGCC[GA] Eco81I 365 CCTCAGG CC^T[ACGT]AGG EcoNI 168 CCTGACGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 17 GGTCACC G^GT[ACGT]ACC EcoRV 658 GATATC GAT^ATC FbaI 1256 TGATCA T^GATCA HgaI 171 GACGC GACGC(5/10) HincII 736 GTCAAC GT[CT]^[GA]AC HindII 736 GTCAAC GT[CT]^[GA]AC HindIII 1400 AAGCTT A^AGCTT KpnI 370 GGTACC GGTAC^C MflI 613 GGATCC [GA]^GATC[CT] MseI 905 TTAA T^TAA MvnI 783 CGCG CG^CG NspV 1341 TTCGAA TT^CGAA PmaCI 835 CACGTG CAC^GTG PmlI 835 CACGTG CAC^GTG ScaI 128 AGTACT AGT^ACT SfuI 1341 TTCGAA TT^CGAA SmaI 789 CCCGGG CCC^GGG SmlI 747 CTCAAG C^T[CT][GA]AG Tru9I 905 TTAA T^TAA XcmI 1311 CCAGGGTGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 613 GGATCC [GA]^GATC[CT] XmaCI 789 CCCGGG C^CCGGG XmaI 789 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AspHI 228 GTGCAC G[AT]GC[AT]^C 336 GTGCTC BanI 370 GGTACC G^G[CT][GA]CC 503 GGCACC BfaI 587 CTAG C^TAG 1078 CTAG BseRI 363 CTCCTC GAGGAG(10/8) 1162 GAGGAG BsgI 195 CTGCAC GTGCAG(16/14) 817 CTGCAC BsiHKAI 228 GTGCAC G[AT]GC[AT]^C 336 GTGCTC BsmAI 945 GAGAC GTCTC(1/5) 1216 GAGAC BspMI 373 ACCTGC ACCTGC(4/8) 807 GCAGGT BsrDI 1035 CATTGC GCAATG(2/0) 650 GCAATG BsrFI 646 GCCGGC [GA]^CCGG[CT] 1279 GCCGGC Cfr10I 646 GCCGGC [GA]^CCGG[CT] 1279 GCCGGC DraIII 505 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG 886 CACTGCGTG DsaI 51 CCATGG C^C[GA][CT]GG 913 CCATGG MaeI 587 CTAG C^TAG 1078 CTAG MslI 679 CACAGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1220 CACAGAGATG MspA1I 302 CCGCTG C[AC]G^C[GT]G 609 CAGCGG NaeI 646 GCCGGC GCC^GGC 1279 GCCGGC NcoI 51 CCATGG C^CATGG 913 CCATGG NgoMIV 646 GCCGGC G^CCGGC 1279 GCCGGC PflMI 25 CCAGCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 885 CCACTGCGTGG PleI 361 GACTC GAGTC(4/5) 1012 GACTC PpuMI 164 GGGTCCT [GA]G^G[AT]CC[CT] 274 GGGACCT SbfI 551 CCTGCAGG CCTGCA^GG 1287 CCTGCAGG Sse8387I 551 CCTGCAGG CCTGCA^GG 1287 CCTGCAGG TfiI 435 GAATC G^A[AT]TC 1303 GATTC Tsp509I 577 AATT ^AATT 1006 AATT Van91I 25 CCAGCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 885 CCACTGCGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AciI AfaI AluI AlwI AvaI AvaII BbvI BciVI BcnI BmyI BsaJI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp45I TspRI TthHB8I