(Circular) (Six-base) MAPSORT of: pFN21AE4470.seq Check: 7010 from: 1 to: 6181 pFN21AE4470 6181 bp With 182 enzymes: SgfI * March 28, 2012 13:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3991 278 Size: 53 83 186 936 2455 2468 Fragments arranged by size: 2468 2455 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2112 3524 1937 Size: 175 1412 4594 Fragments arranged by size: 4594 1412 175 Acc65I G'GTAC_C Cuts at: 2375 3502 2375 Size: 1127 5054 AclI AA'CG_TT Cuts at: 1563 4308 4681 1563 Size: 2745 373 3063 Fragments arranged by size: 3063 2745 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2462 4244 5628 5896 1632 Size: 830 1782 1384 268 1917 Fragments arranged by size: 1917 1782 1384 830 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5984 AflIII A'CryG_T Cuts at: 1203 2841 5080 6057 1203 Size: 1638 2239 977 1327 Fragments arranged by size: 2239 1638 1327 977 AgeI A'CCGG_T Cuts at: 5012 5012 Size: 6181 AhdI GACnn_n'nnGTC Cuts at: 4910 4910 Size: 6181 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6181 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3137 1184 Size: 1953 4228 AlwNI CAG_nnn'CTG Cuts at: 3924 5496 3924 Size: 1572 4609 ApaLI G'TGCA_C Cuts at: 2233 4238 5394 2233 Size: 2005 1156 3020 Fragments arranged by size: 3020 2005 1156 ApoI r'AATT_y Cuts at: 1493 3490 3716 3868 5977 1493 Size: 1997 226 152 2109 1697 Fragments arranged by size: 2109 1997 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4735 160 Size: 872 3703 1606 Fragments arranged by size: 3703 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6181 AvaI C'yCGr_G Cuts at: 1870 1943 2164 2794 2867 3506 1870 Size: 73 221 630 73 639 4545 Fragments arranged by size: 4545 639 630 221 73 73 BamHI G'GATC_C Cuts at: 2618 3511 3878 2618 Size: 893 367 4921 Fragments arranged by size: 4921 893 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2375 2508 3502 4957 618 Size: 325 217 627 588 133 994 1455 1842 Fragments arranged by size: 1842 1455 994 627 588 325 217 133 BanII G_rGCy'C Cuts at: 1391 3500 1391 Size: 2109 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3185 928 Size: 971 1286 3924 Fragments arranged by size: 3924 1286 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4372 4406 1903 Size: 34 2435 34 3678 Fragments arranged by size: 3678 2435 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2054 2972 3370 4073 5283 2054 Size: 918 398 703 1210 2952 Fragments arranged by size: 2952 1210 918 703 398 BclI T'GATC_A Cuts at: 1548 3261 1548 Size: 1713 4468 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4792 136 Size: 107 122 71 631 605 3120 1525 Fragments arranged by size: 3120 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6176 6176 Size: 6181 BlpI GC'TnA_GC Cuts at: 3598 3598 Size: 6181 Bme1580I G_kGCm'C Cuts at: 1790 2154 2237 4242 5398 1790 Size: 364 83 2005 1156 2573 Fragments arranged by size: 2573 2005 1156 364 83 BmgBI CAC'GTC Cuts at: 1318 2827 1318 Size: 1509 4672 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2149 3912 4870 Size: 416 53 83 399 24 732 1763 958 Cuts at: 4870 442 Size: 1753 Fragments arranged by size: 1763 1753 958 732 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6181 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2383 2383 Size: 6181 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4841 1239 Size: 3602 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2737 4306 5171 5469 2737 Size: 1569 865 298 3449 Fragments arranged by size: 3449 1569 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4844 882 Size: 617 3345 2219 Fragments arranged by size: 3345 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2842 3972 493 Size: 711 1638 1130 2702 Fragments arranged by size: 2702 1638 1130 711 BsaBI GATnn'nnATC Cuts at: 6175 6175 Size: 6181 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3988 4370 5005 275 Size: 53 83 186 936 2455 382 635 1451 Fragments arranged by size: 2455 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2068 4614 5012 5286 5433 5844 2068 Size: 2546 398 274 147 411 2405 Fragments arranged by size: 2546 2405 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5956 Fragments arranged by size: 5956 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2359 3182 1186 Size: 1173 823 4185 Fragments arranged by size: 4185 1173 823 BseYI C'CCAG_C Cuts at: 3384 5384 5880 3384 Size: 2000 496 3685 Fragments arranged by size: 3685 2000 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2185 2807 1163 Size: 573 449 622 4537 Fragments arranged by size: 4537 622 573 449 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3631 4392 5420 1540 Size: 275 186 1630 761 1028 2301 Fragments arranged by size: 2301 1630 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2237 2345 4242 4327 5398 2237 Size: 108 1897 85 1071 3020 Fragments arranged by size: 3020 1897 1071 108 85 BsmI GAATG_Cn' Cuts at: 3692 3785 3692 Size: 93 6088 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2154 2237 2345 3500 4242 4327 5398 Size: 399 364 83 108 1155 742 85 1071 Cuts at: 5398 1391 Size: 2174 Fragments arranged by size: 2174 1155 1071 742 399 364 108 85 83 BspEI T'CCGG_A Cuts at: 5844 5844 Size: 6181 BspHI T'CATG_A Cuts at: 4070 5800 4070 Size: 1730 4451 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2387 2803 3536 844 Size: 1543 416 733 3489 Fragments arranged by size: 3489 1543 733 416 BsrBI CCG'CTC Cuts at: 1394 1823 4068 1394 Size: 429 2245 3507 Fragments arranged by size: 3507 2245 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2662 3039 4676 4850 65 Size: 1183 1414 377 1637 174 1396 Fragments arranged by size: 1637 1414 1396 1183 377 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2651 3284 4825 5012 1700 Size: 110 841 633 1541 187 2869 Fragments arranged by size: 2869 1541 841 633 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6181 BssHII G'CGCG_C Cuts at: 1925 2787 1925 Size: 862 5319 BssSI C'ACGA_G Cuts at: 2288 4241 5253 2288 Size: 1953 1012 3216 Fragments arranged by size: 3216 1953 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2340 794 Size: 1546 4635 BstBI TT'CG_AA Cuts at: 3347 4985 3347 Size: 1638 4543 BstEII G'GTnAC_C Cuts at: 1184 2022 1184 Size: 838 5343 BstXI CCAn_nnnn'nTGG Cuts at: 2925 2925 Size: 6181 BstYI r'GATC_y Cuts at: 1972 2618 3511 3878 4263 4280 5732 5818 5830 Size: 646 893 367 385 17 1452 86 12 Cuts at: 5830 6176 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 893 646 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2371 2371 Size: 6181 BtgI C'CryG_G Cuts at: 513 1065 2004 2056 2918 513 Size: 552 939 52 862 3776 Fragments arranged by size: 3776 939 862 552 52 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5442 Fragments arranged by size: 5442 683 56 BtsI GCAGTG_nn' Cuts at: 2416 2715 2890 3697 4491 4511 2416 Size: 299 175 807 794 20 4086 Fragments arranged by size: 4086 807 794 299 175 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6181 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2691 2726 529 Size: 35 2127 35 3984 Fragments arranged by size: 3984 2127 35 35 DraI TTT'AAA Cuts at: 3485 3485 Size: 6181 DraIII CAC_nnn'GTG Cuts at: 2515 2896 2515 Size: 381 5800 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5188 809 Size: 507 3872 1802 Fragments arranged by size: 3872 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2304 3628 4517 Size: 54 1425 50 275 18 474 1324 889 Cuts at: 4517 6146 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1324 889 474 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3628 1537 Size: 275 1816 4090 Fragments arranged by size: 4090 1816 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4111 1353 Size: 2758 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4768 5142 5288 387 Size: 1064 138 3179 374 146 1280 Fragments arranged by size: 3179 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2462 4244 4841 5628 5896 1239 Size: 393 830 1782 597 787 268 1524 Fragments arranged by size: 1782 1524 830 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2177 2177 Size: 6181 EcoO109I rG'GnC_Cy Cuts at: 2170 2280 2799 3332 2170 Size: 110 519 533 5019 Fragments arranged by size: 5019 533 519 110 EcoRI G'AATT_C Cuts at: 3490 3868 3490 Size: 378 5803 EcoRV GAT'ATC Cuts at: 2665 2665 Size: 6181 FspI TGC'GCA Cuts at: 4687 4687 Size: 6181 HaeII r_GCGC'y Cuts at: 1678 5328 1678 Size: 3650 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2396 2428 2603 2635 2841 Size: 32 135 32 824 32 175 32 206 Cuts at: 2841 2873 2989 3021 4822 4854 4896 4928 1373 Size: 32 116 32 1801 32 42 32 2626 Fragments arranged by size: 2626 1801 824 206 175 135 116 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2743 3525 3771 669 Size: 988 281 805 782 246 3079 Fragments arranged by size: 3079 988 805 782 281 246 HindIII A'AGCT_T Cuts at: 748 3405 748 Size: 2657 3524 HpaI GTT'AAC Cuts at: 3771 3771 Size: 6181 KpnI G_GTAC'C Cuts at: 2379 3506 2379 Size: 1127 5054 MfeI C'AATT_G Cuts at: 3780 3780 Size: 6181 MluI A'CGCG_T Cuts at: 6057 6057 Size: 6181 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2211 2370 5295 5479 1305 Size: 906 159 2925 184 2007 Fragments arranged by size: 2925 2007 906 184 159 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4359 Fragments arranged by size: 4359 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2688 3229 4139 4498 4657 518 Size: 648 1522 541 910 359 159 2042 Fragments arranged by size: 2042 1522 910 648 541 359 159 MspA1I CmG'CkG Cuts at: 1606 2309 2616 3597 4274 5422 5667 1606 Size: 703 307 981 677 1148 245 2120 Fragments arranged by size: 2120 1148 981 703 677 307 245 NaeI GCC'GGC Cuts at: 2653 3286 2653 Size: 633 5548 NcoI C'CATG_G Cuts at: 513 1065 2004 2056 2918 513 Size: 552 939 52 862 3776 Fragments arranged by size: 3776 939 862 552 52 NdeI CA'TA_TG Cuts at: 387 387 Size: 6181 NgoMIV G'CCGG_C Cuts at: 2651 3284 2651 Size: 633 5548 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6181 NotI GC'GGCC_GC Cuts at: 3628 3628 Size: 6181 NspI r_CATG'y Cuts at: 1132 1229 3539 5084 1132 Size: 97 2310 1545 2229 Fragments arranged by size: 2310 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2275 1348 Size: 927 5254 PciI A'CATG_T Cuts at: 5080 5080 Size: 6181 PflMI CCAn_nnn'nTGG Cuts at: 1108 2036 2896 1108 Size: 928 860 4393 Fragments arranged by size: 4393 928 860 PfoI T'CCnGG_A Cuts at: 2222 2222 Size: 6181 PmeI GTTT'AAAC Cuts at: 3485 3485 Size: 6181 PmlI CAC'GTG Cuts at: 2842 2842 Size: 6181 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4250 4282 5763 5795 4250 Size: 32 1481 32 4636 Fragments arranged by size: 4636 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2170 2280 2170 Size: 110 6071 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6181 PsiI TTA'TAA Cuts at: 3751 3751 Size: 6181 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2525 2557 2525 Size: 32 6149 PstI C_TGCA'G Cuts at: 830 2561 3060 3126 3297 3533 830 Size: 1731 499 66 171 236 3478 Fragments arranged by size: 3478 1731 499 236 171 66 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6181 SalI G'TCGA_C Cuts at: 1936 3523 1936 Size: 1587 4594 SbfI CC_TGCA'GG Cuts at: 2561 3297 3533 2561 Size: 736 236 5209 Fragments arranged by size: 5209 736 236 ScaI AGT'ACT Cuts at: 2135 4429 6141 2135 Size: 2294 1712 2175 Fragments arranged by size: 2294 2175 1712 SfcI C'TryA_G Cuts at: 826 1045 2557 3056 3122 3293 3529 4664 5345 Size: 219 1512 499 66 171 236 1135 681 Cuts at: 5345 5536 826 Size: 191 1471 Fragments arranged by size: 1512 1471 1135 681 499 236 219 191 171 66 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6181 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6181 SmaI CCC'GGG Cuts at: 1872 2796 3508 1872 Size: 924 712 4545 Fragments arranged by size: 4545 924 712 SmlI C'TyrA_G Cuts at: 820 1017 1943 2752 4285 5186 5448 820 Size: 197 926 809 1533 901 262 1553 Fragments arranged by size: 1553 1533 926 901 809 262 197 SnaBI TAC'GTA Cuts at: 493 3972 493 Size: 3479 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6181 SphI G_CATG'C Cuts at: 1132 3539 1132 Size: 2407 3774 SspI AAT'ATT Cuts at: 5 52 4105 5 Size: 47 4053 2081 Fragments arranged by size: 4053 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6181 StyI C'CwwG_G Cuts at: 513 1065 2004 2056 2596 2918 3204 3620 513 Size: 552 939 52 540 322 286 416 3074 Fragments arranged by size: 3074 939 552 540 416 322 286 52 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4208 4225 4378 4563 5008 325 Size: 1255 2628 17 153 185 445 1498 Fragments arranged by size: 2628 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2133 2484 4427 Size: 274 80 33 51 1443 156 351 1943 Cuts at: 4427 6139 96 Size: 1712 138 Fragments arranged by size: 1943 1712 1443 351 274 156 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6141 6173 6141 Size: 32 6149 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6181 XbaI T'CTAG_A Cuts at: 3517 3517 Size: 6181 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3323 3323 Size: 6181 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6181 XmaI C'CCGG_G Cuts at: 1870 2794 3506 1870 Size: 924 712 4545 Fragments arranged by size: 4545 924 712 XmnI GAAnn'nnTTC Cuts at: 4310 4310 Size: 6181 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3989 276 Size: 53 83 186 936 2455 2468 Fragments arranged by size: 2468 2455 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I EcoICRI FalI FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI