Restriction map for
pFN21AE4470
(Linear) (Six-base) MAPSORT of: KIEE4470.seq Check: 6900 from: 1 to: 1476
KIEE4470.orf
With 182 enzymes: SgfI *
March 22, 2012 18:08 ..
AccI GT'mk_AC
Cuts at: 0 107 1476
Size: 107 1369
Acc65I G'GTAC_C
Cuts at: 0 370 1476
Size: 370 1106
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 1476
Size: 457 1019
AflIII A'CryG_T
Cuts at: 0 836 1476
Size: 836 640
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1132 1476
Size: 1132 344
ApaLI G'TGCA_C
Cuts at: 0 228 1476
Size: 228 1248
AvaI C'yCGr_G
Cuts at: 0 159 789 862 1476
Size: 159 630 73 614
Fragments arranged by size:
630 614 159 73
BamHI G'GATC_C
Cuts at: 0 613 1476
Size: 613 863
BanI G'GyrC_C
Cuts at: 0 370 503 1476
Size: 370 133 973
Fragments arranged by size:
973 370 133
BbsI GAAGACnn'nnnn_
Cuts at: 0 1180 1476
Size: 1180 296
BciVI GTATCCnnnnn_n'
Cuts at: 0 49 967 1365 1476
Size: 49 918 398 111
Fragments arranged by size:
918 398 111 49
BclI T'GATC_A
Cuts at: 0 1256 1476
Size: 1256 220
Bme1580I G_kGCm'C
Cuts at: 0 149 232 1476
Size: 149 83 1244
Fragments arranged by size:
1244 149 83
BmgBI CAC'GTC
Cuts at: 0 822 1476
Size: 822 654
BmrI ACTGGGnnnn_n'
Cuts at: 0 144 1476
Size: 144 1332
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 378 1476
Size: 378 1098
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 732 1476
Size: 732 744
BsaAI yAC'GTr
Cuts at: 0 837 1476
Size: 837 639
BsaWI w'CCGG_w
Cuts at: 0 63 1476
Size: 63 1413
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 354 1177 1476
Size: 354 823 299
Fragments arranged by size:
823 354 299
BseYI C'CCAG_C
Cuts at: 0 1379 1476
Size: 1379 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 180 802 1476
Size: 180 622 674
Fragments arranged by size:
674 622 180
BsiHKAI G_wGCw'C
Cuts at: 0 232 340 1476
Size: 232 108 1136
Fragments arranged by size:
1136 232 108
Bsp1286I G_dGCh'C
Cuts at: 0 149 232 340 1476
Size: 149 83 108 1136
Fragments arranged by size:
1136 149 108 83
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 382 798 1476
Size: 382 416 678
Fragments arranged by size:
678 416 382
BsrDI GCAATG_nn'
Cuts at: 0 657 1034 1476
Size: 657 377 442
Fragments arranged by size:
657 442 377
BsrFI r'CCGG_y
Cuts at: 0 646 1279 1476
Size: 646 633 197
Fragments arranged by size:
646 633 197
BssHII G'CGCG_C
Cuts at: 0 782 1476
Size: 782 694
BssSI C'ACGA_G
Cuts at: 0 283 1476
Size: 283 1193
BstAPI GCAn_nnn'nTGC
Cuts at: 0 335 1476
Size: 335 1141
BstBI TT'CG_AA
Cuts at: 0 1342 1476
Size: 1342 134
BstEII G'GTnAC_C
Cuts at: 0 17 1476
Size: 17 1459
BstXI CCAn_nnnn'nTGG
Cuts at: 0 920 1476
Size: 920 556
BstYI r'GATC_y
Cuts at: 0 613 1476
Size: 613 863
Bsu36I CC'TnA_GG
Cuts at: 0 366 1476
Size: 366 1110
BtgI C'CryG_G
Cuts at: 0 51 913 1476
Size: 51 862 563
Fragments arranged by size:
862 563 51
BtsI GCAGTG_nn'
Cuts at: 0 411 710 885 1476
Size: 411 299 175 591
Fragments arranged by size:
591 411 299 175
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 686 721 1476
Size: 686 35 755
Fragments arranged by size:
755 686 35
DraIII CAC_nnn'GTG
Cuts at: 0 510 891 1476
Size: 510 381 585
Fragments arranged by size:
585 510 381
EaeI y'GGCC_r
Cuts at: 0 299 1476
Size: 299 1177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 1476
Size: 457 1019
EcoNI CCTnn'n_nnAGG
Cuts at: 0 172 1476
Size: 172 1304
EcoO109I rG'GnC_Cy
Cuts at: 0 165 275 794 1327 1476
Size: 165 110 519 533 149
Fragments arranged by size:
533 519 165 149 110
EcoRV GAT'ATC
Cuts at: 0 660 1476
Size: 660 816
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 391 423 598 630 836 868 984 1016
Size: 391 32 175 32 206 32 116 32
Cuts at: 1016 1476
Size: 460
Fragments arranged by size:
460 391 206 175 116 32 32 32
32
HincII GTy'rAC
Cuts at: 0 738 1476
Size: 738 738
HindIII A'AGCT_T
Cuts at: 0 1400 1476
Size: 1400 76
KpnI G_GTAC'C
Cuts at: 0 374 1476
Size: 374 1102
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 365 1476
Size: 206 159 1111
Fragments arranged by size:
1111 206 159
MslI CAynn'nnrTG
Cuts at: 0 683 1224 1476
Size: 683 541 252
Fragments arranged by size:
683 541 252
MspA1I CmG'CkG
Cuts at: 0 304 611 1476
Size: 304 307 865
Fragments arranged by size:
865 307 304
NaeI GCC'GGC
Cuts at: 0 648 1281 1476
Size: 648 633 195
Fragments arranged by size:
648 633 195
NcoI C'CATG_G
Cuts at: 0 51 913 1476
Size: 51 862 563
Fragments arranged by size:
862 563 51
NgoMIV G'CCGG_C
Cuts at: 0 646 1279 1476
Size: 646 633 197
Fragments arranged by size:
646 633 197
PasI CC'CwG_GG
Cuts at: 0 270 1476
Size: 270 1206
PflMI CCAn_nnn'nTGG
Cuts at: 0 31 891 1476
Size: 31 860 585
Fragments arranged by size:
860 585 31
PfoI T'CCnGG_A
Cuts at: 0 217 1476
Size: 217 1259
PmlI CAC'GTG
Cuts at: 0 837 1476
Size: 837 639
PpuMI rG'GwC_Cy
Cuts at: 0 165 275 1476
Size: 165 110 1201
Fragments arranged by size:
1201 165 110
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 520 552 1476
Size: 520 32 924
Fragments arranged by size:
924 520 32
PstI C_TGCA'G
Cuts at: 0 556 1055 1121 1292 1476
Size: 556 499 66 171 184
Fragments arranged by size:
556 499 184 171 66
SbfI CC_TGCA'GG
Cuts at: 0 556 1292 1476
Size: 556 736 184
Fragments arranged by size:
736 556 184
ScaI AGT'ACT
Cuts at: 0 130 1476
Size: 130 1346
SfcI C'TryA_G
Cuts at: 0 552 1051 1117 1288 1476
Size: 552 499 66 171 188
Fragments arranged by size:
552 499 188 171 66
SmaI CCC'GGG
Cuts at: 0 791 1476
Size: 791 685
SmlI C'TyrA_G
Cuts at: 0 747 1476
Size: 747 729
StyI C'CwwG_G
Cuts at: 0 51 591 913 1199 1476
Size: 51 540 322 286 277
Fragments arranged by size:
540 322 286 277 51
TatI w'GTAC_w
Cuts at: 0 128 479 1476
Size: 128 351 997
Fragments arranged by size:
997 351 128
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1318 1476
Size: 1318 158
XmaI C'CCGG_G
Cuts at: 0 789 1476
Size: 789 687
Enzymes that do cut:
AccI Acc65I AcuI AflIII AlfI ApaLI AvaI BamHI
BanI BbsI BciVI BclI Bme1580I BmgBI BmrI BplI
BpuEI BsaAI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtsI CspCI DraIII EaeI
Eco57MI EcoNI EcoO109I EcoRV Hin4I HincII HindIII KpnI
MmeI MslI MspA1I NaeI NcoI NgoMIV PasI PflMI
PfoI PmlI PpuMI PsrI PstI SbfI ScaI SfcI
SmaI SmlI StyI TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AleI
AloI AlwNI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BanII BbeI BbvCI BcgI BfrBI BglI BglII
BlpI BmtI BpmI Bpu10I BsaI BsaBI BsaHI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI
BstZ17I BtgZI ClaI DraI DrdI EagI EarI EciI
EcoICRI EcoRI FalI FseI FspI FspAI HaeII HpaI
KasI MfeI MluI MscI NarI NdeI NheI NotI
NruI NsiI NspI PacI PciI PmeI PpiI PshAI
PsiI PspOMI PspXI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TstI Tth111I XbaI XhoI XmnI ZraI