(Circular) (Six-base) MAPSORT of: pFN21AE2699.seq Check: 4799 from: 1 to: 7459 pFN21AE2699 7459 bp With 182 enzymes: SgfI * July 6, 2017 14:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5269 278 Size: 53 83 186 936 3733 2468 Fragments arranged by size: 3733 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2560 4802 1937 Size: 623 2242 4594 Fragments arranged by size: 4594 2242 623 Acc65I G'GTAC_C Cuts at: 3689 4780 3689 Size: 1091 6368 AclI AA'CG_TT Cuts at: 1563 5586 5959 1563 Size: 4023 373 3063 Fragments arranged by size: 4023 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2982 5522 6906 7174 1632 Size: 1350 2540 1384 268 1917 Fragments arranged by size: 2540 1917 1384 1350 268 AflII C'TTAA_G Cuts at: 820 1017 2723 820 Size: 197 1706 5556 Fragments arranged by size: 5556 1706 197 AflIII A'CryG_T Cuts at: 1203 2871 6358 7335 1203 Size: 1668 3487 977 1327 Fragments arranged by size: 3487 1668 1327 977 AgeI A'CCGG_T Cuts at: 6290 6290 Size: 7459 AhdI GACnn_n'nnGTC Cuts at: 6188 6188 Size: 7459 AleI CACnn'nnGTG Cuts at: 1166 3611 1166 Size: 2445 5014 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7459 AlwNI CAG_nnn'CTG Cuts at: 2714 2734 5202 6774 2714 Size: 20 2468 1572 3399 Fragments arranged by size: 3399 2468 1572 20 ApaI G_GGCC'C Cuts at: 3523 3523 Size: 7459 ApaLI G'TGCA_C Cuts at: 5516 6672 5516 Size: 1156 6303 ApoI r'AATT_y Cuts at: 1493 3268 4571 4768 4994 5146 7255 1493 Size: 1775 1303 197 226 152 2109 1697 Fragments arranged by size: 2109 1775 1697 1303 226 197 152 AseI AT'TA_AT Cuts at: 160 1032 4709 6013 160 Size: 872 3677 1304 1606 Fragments arranged by size: 3677 1606 1304 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7459 AvaI C'yCGr_G Cuts at: 1870 1943 2033 2927 4463 4784 1870 Size: 73 90 894 1536 321 4545 Fragments arranged by size: 4545 1536 894 321 90 73 AvrII C'CTAG_G Cuts at: 3655 3655 Size: 7459 BamHI G'GATC_C Cuts at: 4221 4789 5156 4221 Size: 568 367 6524 Fragments arranged by size: 6524 568 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3066 3689 4780 6235 618 Size: 325 217 627 1279 623 1091 1455 1842 Fragments arranged by size: 1842 1455 1279 1091 627 623 325 217 BanII G_rGCy'C Cuts at: 1391 2040 2571 3523 3904 4778 1391 Size: 649 531 952 381 874 4072 Fragments arranged by size: 4072 952 874 649 531 381 BbeI G_GCGC'C Cuts at: 3070 3070 Size: 7459 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2544 4476 928 Size: 971 645 1932 3911 Fragments arranged by size: 3911 1932 971 645 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2005 2039 2362 2396 5650 5684 1903 Size: 34 68 34 323 34 3254 34 3678 Fragments arranged by size: 3678 3254 323 68 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5351 6561 5351 Size: 1210 6249 BclI T'GATC_A Cuts at: 1548 3093 1548 Size: 1545 5914 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4013 6070 136 Size: 107 122 71 631 605 2341 2057 1525 Fragments arranged by size: 2341 2057 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7454 7454 Size: 7459 BlpI GC'TnA_GC Cuts at: 4876 4876 Size: 7459 Bme1580I G_kGCm'C Cuts at: 1790 2065 3523 4492 5520 6676 1790 Size: 275 1458 969 1028 1156 2573 Fragments arranged by size: 2573 1458 1156 1028 969 275 BmgBI CAC'GTC Cuts at: 1318 3850 1318 Size: 2532 4927 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3132 5190 6148 Size: 416 53 83 399 24 1715 2058 958 Cuts at: 6148 442 Size: 1753 Fragments arranged by size: 2058 1753 1715 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7459 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2569 3714 6119 1239 Size: 1330 1145 2405 2579 Fragments arranged by size: 2579 2405 1330 1145 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2636 3175 5584 6449 6747 2636 Size: 539 2409 865 298 3348 Fragments arranged by size: 3348 2409 865 539 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6122 882 Size: 617 4623 2219 Fragments arranged by size: 4623 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2872 3613 5250 493 Size: 711 1668 741 1637 2702 Fragments arranged by size: 2702 1668 1637 741 711 BsaBI GATnn'nnATC Cuts at: 3168 7453 3168 Size: 4285 3174 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3067 5266 5648 6283 Size: 53 83 186 936 1534 2199 382 635 Cuts at: 6283 275 Size: 1451 Fragments arranged by size: 2199 1534 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3675 5892 6290 6564 6711 7122 3675 Size: 2217 398 274 147 411 4012 Fragments arranged by size: 4012 2217 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2278 2308 1178 Size: 30 165 30 875 30 6329 Fragments arranged by size: 6329 875 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2012 2057 2335 3585 4733 1186 Size: 826 45 278 1250 1148 3912 Fragments arranged by size: 3912 1250 1148 826 278 45 BseYI C'CCAG_C Cuts at: 2028 2058 3021 6662 7158 2028 Size: 30 963 3641 496 2329 Fragments arranged by size: 3641 2329 963 496 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2381 1163 Size: 573 645 6241 Fragments arranged by size: 6241 645 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4909 5670 6698 1540 Size: 275 186 2908 761 1028 2301 Fragments arranged by size: 2908 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2013 2571 3753 3904 4236 5520 5605 6676 2013 Size: 558 1182 151 332 1284 85 1071 2796 Fragments arranged by size: 2796 1284 1182 1071 558 332 151 85 BsmI GAATG_Cn' Cuts at: 3666 4970 5063 3666 Size: 1304 93 6062 Fragments arranged by size: 6062 1304 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2040 2065 2571 3523 3753 3904 Size: 399 223 27 25 506 952 230 151 Cuts at: 3904 4236 4492 4778 5520 5605 6676 1391 Size: 332 256 286 742 85 1071 2174 Fragments arranged by size: 2174 1071 952 742 506 399 332 286 256 230 223 151 85 27 25 BspEI T'CCGG_A Cuts at: 7122 7122 Size: 7459 BspHI T'CATG_A Cuts at: 3648 4173 5348 7078 3648 Size: 525 1175 1730 4029 Fragments arranged by size: 4029 1730 1175 525 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4814 844 Size: 3970 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5346 1394 Size: 429 3523 3507 Fragments arranged by size: 3523 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2380 3000 4643 5954 6128 65 Size: 1183 1132 620 1643 1311 174 1396 Fragments arranged by size: 1643 1396 1311 1183 1132 620 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6103 6290 1700 Size: 110 4293 187 2869 Fragments arranged by size: 4293 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2988 3387 96 Size: 2892 399 4168 Fragments arranged by size: 4168 2892 399 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7459 BssSI C'ACGA_G Cuts at: 5519 6531 5519 Size: 1012 6447 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7459 BstBI TT'CG_AA Cuts at: 4732 6263 4732 Size: 1531 5928 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7459 BstXI CCAn_nnnn'nTGG Cuts at: 3367 3738 4492 3367 Size: 371 754 6334 Fragments arranged by size: 6334 754 371 BstYI r'GATC_y Cuts at: 1972 2491 2800 4221 4789 5156 5541 5558 7010 Size: 519 309 1421 568 367 385 17 1452 Cuts at: 7010 7096 7108 7454 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1421 568 519 385 367 346 309 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2898 3062 513 Size: 552 939 894 164 4910 Fragments arranged by size: 4910 939 894 552 164 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6720 Fragments arranged by size: 6720 683 56 BtsI GCAGTG_nn' Cuts at: 3050 3594 4975 5769 5789 3050 Size: 544 1381 794 20 4720 Fragments arranged by size: 4720 1381 794 544 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7459 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4255 4290 529 Size: 35 3691 35 3698 Fragments arranged by size: 3698 3691 35 35 DraI TTT'AAA Cuts at: 4763 4763 Size: 7459 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6466 809 Size: 507 5150 1802 Fragments arranged by size: 5150 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2997 3336 4005 Size: 54 1425 50 275 18 1167 339 669 Cuts at: 4005 4906 5795 7424 8 Size: 901 889 1629 43 Fragments arranged by size: 1629 1425 1167 901 889 669 339 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4906 1537 Size: 275 3094 4090 Fragments arranged by size: 4090 3094 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5389 1353 Size: 4036 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2600 6046 6420 6566 387 Size: 1064 138 1011 3446 374 146 1280 Fragments arranged by size: 3446 1280 1064 1011 374 146 138 EcoICRI GAG'CTC Cuts at: 2569 3902 2569 Size: 1333 6126 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2569 2982 3714 5522 6119 6906 7174 Size: 393 937 413 732 1808 597 787 268 Cuts at: 7174 1239 Size: 1524 Fragments arranged by size: 1808 1524 937 787 732 597 413 393 268 EcoO109I rG'GnC_Cy Cuts at: 2914 3519 4318 2914 Size: 605 799 6055 Fragments arranged by size: 6055 799 605 EcoRI G'AATT_C Cuts at: 3268 4571 4768 5146 3268 Size: 1303 197 378 5581 Fragments arranged by size: 5581 1303 378 197 FspI TGC'GCA Cuts at: 5965 5965 Size: 7459 HaeII r_GCGC'y Cuts at: 1678 3070 6606 1678 Size: 1392 3536 2531 Fragments arranged by size: 3536 2531 1392 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2476 2508 2879 2911 6100 Size: 32 135 32 904 32 371 32 3189 Cuts at: 6100 6132 6174 6206 1373 Size: 32 42 32 2626 Fragments arranged by size: 3189 2626 904 371 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4803 5049 669 Size: 988 281 2865 246 3079 Fragments arranged by size: 3079 2865 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7459 HpaI GTT'AAC Cuts at: 5049 5049 Size: 7459 KasI G'GCGC_C Cuts at: 3066 3066 Size: 7459 KpnI G_GTAC'C Cuts at: 3693 4784 3693 Size: 1091 6368 MfeI C'AATT_G Cuts at: 5058 5058 Size: 7459 MluI A'CGCG_T Cuts at: 7335 7335 Size: 7459 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3297 3877 3937 4679 6573 6757 1305 Size: 1992 580 60 742 1894 184 2007 Fragments arranged by size: 2007 1992 1894 742 580 184 60 MscI TGG'CCA Cuts at: 10 64 1489 1832 2999 3338 10 Size: 54 1425 343 1167 339 4131 Fragments arranged by size: 4131 1425 1167 343 339 54 MslI CAynn'nnrTG Cuts at: 518 1166 2744 3611 5417 5776 5935 518 Size: 648 1578 867 1806 359 159 2042 Fragments arranged by size: 2042 1806 1578 867 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2058 2302 2406 2617 2714 3025 3774 4875 Size: 452 244 104 211 97 311 749 1101 Cuts at: 4875 5552 6700 6945 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 1101 749 677 452 311 245 244 211 104 97 NarI GG'CG_CC Cuts at: 3067 3067 Size: 7459 NcoI C'CATG_G Cuts at: 513 1065 2004 2898 3062 513 Size: 552 939 894 164 4910 Fragments arranged by size: 4910 939 894 552 164 NdeI CA'TA_TG Cuts at: 387 4425 387 Size: 4038 3421 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7459 NotI GC'GGCC_GC Cuts at: 4906 4906 Size: 7459 NspI r_CATG'y Cuts at: 1132 1229 2295 4817 6362 1132 Size: 97 1066 2522 1545 2229 Fragments arranged by size: 2522 2229 1545 1066 97 PasI CC'CwG_GG Cuts at: 1348 4492 1348 Size: 3144 4315 PciI A'CATG_T Cuts at: 6358 6358 Size: 7459 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7459 PfoI T'CCnGG_A Cuts at: 2487 2487 Size: 7459 PmeI GTTT'AAAC Cuts at: 4763 4763 Size: 7459 PmlI CAC'GTG Cuts at: 2872 2872 Size: 7459 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5528 5560 7041 7073 5528 Size: 32 1481 32 5914 Fragments arranged by size: 5914 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7459 PsiI TTA'TAA Cuts at: 5029 5029 Size: 7459 PspOMI G'GGCC_C Cuts at: 3519 3519 Size: 7459 PstI C_TGCA'G Cuts at: 830 2913 3045 4811 830 Size: 2083 132 1766 3478 Fragments arranged by size: 3478 2083 1766 132 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7459 PvuII CAG'CTG Cuts at: 2058 2302 2617 3025 3774 2058 Size: 244 315 408 749 5743 Fragments arranged by size: 5743 749 408 315 244 SacI G_AGCT'C Cuts at: 2571 3904 2571 Size: 1333 6126 SalI G'TCGA_C Cuts at: 1936 4801 1936 Size: 2865 4594 SbfI CC_TGCA'GG Cuts at: 4811 4811 Size: 7459 ScaI AGT'ACT Cuts at: 2112 2759 5707 7419 2112 Size: 647 2948 1712 2152 Fragments arranged by size: 2948 2152 1712 647 SfcI C'TryA_G Cuts at: 826 1045 2812 2909 3041 4807 5942 6623 6814 Size: 219 1767 97 132 1766 1135 681 191 Cuts at: 6814 826 Size: 1471 Fragments arranged by size: 1767 1766 1471 1135 681 219 191 132 97 SfoI GGC'GCC Cuts at: 3068 3068 Size: 7459 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7459 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7459 SmaI CCC'GGG Cuts at: 1872 2035 4786 1872 Size: 163 2751 4545 Fragments arranged by size: 4545 2751 163 SmlI C'TyrA_G Cuts at: 820 1017 1943 2651 2723 3154 5563 6464 6726 Size: 197 926 708 72 431 2409 901 262 Cuts at: 6726 820 Size: 1553 Fragments arranged by size: 2409 1553 926 901 708 431 262 197 72 SnaBI TAC'GTA Cuts at: 493 5250 493 Size: 4757 2702 SpeI A'CTAG_T Cuts at: 152 3496 152 Size: 3344 4115 SphI G_CATG'C Cuts at: 1132 4817 1132 Size: 3685 3774 SrfI GCCC'GGGC Cuts at: 2035 2035 Size: 7459 SspI AAT'ATT Cuts at: 5 52 2596 4507 5383 5 Size: 47 2544 1911 876 2081 Fragments arranged by size: 2544 2081 1911 876 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7459 StyI C'CwwG_G Cuts at: 513 1065 2004 2898 3062 3655 4898 513 Size: 552 939 894 164 593 1243 3074 Fragments arranged by size: 3074 1243 939 894 593 552 164 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2768 2935 3843 5486 5503 5656 5841 Size: 1255 1188 167 908 1643 17 153 185 Cuts at: 5841 6286 325 Size: 445 1498 Fragments arranged by size: 1643 1498 1255 1188 908 445 185 167 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2110 2757 2988 Size: 274 80 33 51 1443 133 647 231 Cuts at: 2988 3387 3778 5705 7417 96 Size: 399 391 1927 1712 138 Fragments arranged by size: 1927 1712 1443 647 399 391 274 231 138 133 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2278 2310 2861 2893 3002 3034 7419 7451 2278 Size: 32 551 32 109 32 4385 32 2286 Fragments arranged by size: 4385 2286 551 109 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7459 XbaI T'CTAG_A Cuts at: 4795 4795 Size: 7459 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2306 2621 2306 Size: 315 7144 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7459 XmaI C'CCGG_G Cuts at: 1870 2033 4784 1870 Size: 163 2751 4545 Fragments arranged by size: 4545 2751 163 XmnI GAAnn'nnTTC Cuts at: 5588 5588 Size: 7459 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5267 276 Size: 53 83 186 936 3733 2468 Fragments arranged by size: 3733 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SwaI