(Linear) (Six-base) MAPSORT of: KIEE2699.seq Check: 8352 from: 1 to: 2754 KIEE2699.orf With 182 enzymes: SgfI * July 6, 2017 13:31 .. AccI GT'mk_AC Cuts at: 0 555 2754 Size: 555 2199 Acc65I G'GTAC_C Cuts at: 0 1684 2754 Size: 1684 1070 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 977 2754 Size: 977 1777 AflII C'TTAA_G Cuts at: 0 718 2754 Size: 718 2036 AflIII A'CryG_T Cuts at: 0 866 2754 Size: 866 1888 AleI CACnn'nnGTG Cuts at: 0 1606 2754 Size: 1606 1148 AlwNI CAG_nnn'CTG Cuts at: 0 709 729 2754 Size: 709 20 2025 Fragments arranged by size: 2025 709 20 ApaI G_GGCC'C Cuts at: 0 1518 2754 Size: 1518 1236 ApoI r'AATT_y Cuts at: 0 1263 2566 2754 Size: 1263 1303 188 Fragments arranged by size: 1303 1263 188 AseI AT'TA_AT Cuts at: 0 2704 2754 Size: 2704 50 AvaI C'yCGr_G Cuts at: 0 28 922 2458 2754 Size: 28 894 1536 296 Fragments arranged by size: 1536 894 296 28 AvrII C'CTAG_G Cuts at: 0 1650 2754 Size: 1650 1104 BamHI G'GATC_C Cuts at: 0 2216 2754 Size: 2216 538 BanI G'GyrC_C Cuts at: 0 1061 1684 2754 Size: 1061 623 1070 Fragments arranged by size: 1070 1061 623 BanII G_rGCy'C Cuts at: 0 35 566 1518 1899 2754 Size: 35 531 952 381 855 Fragments arranged by size: 952 855 531 381 35 BbeI G_GCGC'C Cuts at: 0 1065 2754 Size: 1065 1689 BbsI GAAGACnn'nnnn_ Cuts at: 0 539 2471 2754 Size: 539 1932 283 Fragments arranged by size: 1932 539 283 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 34 357 391 2754 Size: 34 323 34 2363 Fragments arranged by size: 2363 323 34 34 BclI T'GATC_A Cuts at: 0 1088 2754 Size: 1088 1666 BglI GCCn_nnn'nGGC Cuts at: 0 2008 2754 Size: 2008 746 Bme1580I G_kGCm'C Cuts at: 0 60 1518 2487 2754 Size: 60 1458 969 267 Fragments arranged by size: 1458 969 267 60 BmgBI CAC'GTC Cuts at: 0 1845 2754 Size: 1845 909 BmrI ACTGGGnnnn_n' Cuts at: 0 1127 2754 Size: 1127 1627 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 564 1709 2754 Size: 564 1145 1045 Fragments arranged by size: 1145 1045 564 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 631 1170 2754 Size: 631 539 1584 Fragments arranged by size: 1584 631 539 BsaAI yAC'GTr Cuts at: 0 867 1608 2754 Size: 867 741 1146 Fragments arranged by size: 1146 867 741 BsaBI GATnn'nnATC Cuts at: 0 1163 2754 Size: 1163 1591 BsaHI Gr'CG_yC Cuts at: 0 1062 2754 Size: 1062 1692 BsaWI w'CCGG_w Cuts at: 0 1670 2754 Size: 1670 1084 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 273 303 2754 Size: 273 30 2451 Fragments arranged by size: 2451 273 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 7 52 330 1580 2728 2754 Size: 7 45 278 1250 1148 26 Fragments arranged by size: 1250 1148 278 45 26 7 BseYI C'CCAG_C Cuts at: 0 23 53 1016 2754 Size: 23 30 963 1738 Fragments arranged by size: 1738 963 30 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 376 2754 Size: 376 2378 BsiHKAI G_wGCw'C Cuts at: 0 8 566 1748 1899 2231 2754 Size: 8 558 1182 151 332 523 Fragments arranged by size: 1182 558 523 332 151 8 BsmI GAATG_Cn' Cuts at: 0 1661 2754 Size: 1661 1093 Bsp1286I G_dGCh'C Cuts at: 0 8 35 60 566 1518 1748 1899 2231 Size: 8 27 25 506 952 230 151 332 Cuts at: 2231 2487 2754 Size: 256 267 Fragments arranged by size: 952 506 332 267 256 230 151 27 25 8 BspHI T'CATG_A Cuts at: 0 1643 2168 2754 Size: 1643 525 586 Fragments arranged by size: 1643 586 525 BsrDI GCAATG_nn' Cuts at: 0 375 995 2638 2754 Size: 375 620 1643 116 Fragments arranged by size: 1643 620 375 116 BsrGI T'GTAC_A Cuts at: 0 983 1382 2754 Size: 983 399 1372 Fragments arranged by size: 1372 983 399 BstBI TT'CG_AA Cuts at: 0 2727 2754 Size: 2727 27 BstXI CCAn_nnnn'nTGG Cuts at: 0 1362 1733 2487 2754 Size: 1362 371 754 267 Fragments arranged by size: 1362 754 371 267 BstYI r'GATC_y Cuts at: 0 486 795 2216 2754 Size: 486 309 1421 538 Fragments arranged by size: 1421 538 486 309 BtgI C'CryG_G Cuts at: 0 893 1057 2754 Size: 893 164 1697 Fragments arranged by size: 1697 893 164 BtsI GCAGTG_nn' Cuts at: 0 1045 1589 2754 Size: 1045 544 1165 Fragments arranged by size: 1165 1045 544 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2250 2285 2754 Size: 2250 35 469 Fragments arranged by size: 2250 469 35 EaeI y'GGCC_r Cuts at: 0 992 1331 2000 2754 Size: 992 339 669 754 Fragments arranged by size: 992 754 669 339 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 595 2754 Size: 595 2159 EcoICRI GAG'CTC Cuts at: 0 564 1897 2754 Size: 564 1333 857 Fragments arranged by size: 1333 857 564 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 564 977 1709 2754 Size: 564 413 732 1045 Fragments arranged by size: 1045 732 564 413 EcoO109I rG'GnC_Cy Cuts at: 0 909 1514 2313 2754 Size: 909 605 799 441 Fragments arranged by size: 909 799 605 441 EcoRI G'AATT_C Cuts at: 0 1263 2566 2754 Size: 1263 1303 188 Fragments arranged by size: 1303 1263 188 HaeII r_GCGC'y Cuts at: 0 1065 2754 Size: 1065 1689 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 471 503 874 906 2754 Size: 471 32 371 32 1848 Fragments arranged by size: 1848 471 371 32 32 KasI G'GCGC_C Cuts at: 0 1061 2754 Size: 1061 1693 KpnI G_GTAC'C Cuts at: 0 1688 2754 Size: 1688 1066 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1292 1872 1932 2674 2754 Size: 1292 580 60 742 80 Fragments arranged by size: 1292 742 580 80 60 MscI TGG'CCA Cuts at: 0 994 1333 2754 Size: 994 339 1421 Fragments arranged by size: 1421 994 339 MslI CAynn'nnrTG Cuts at: 0 739 1606 2754 Size: 739 867 1148 Fragments arranged by size: 1148 867 739 MspA1I CmG'CkG Cuts at: 0 53 297 401 612 709 1020 1769 2754 Size: 53 244 104 211 97 311 749 985 Fragments arranged by size: 985 749 311 244 211 104 97 53 NarI GG'CG_CC Cuts at: 0 1062 2754 Size: 1062 1692 NcoI C'CATG_G Cuts at: 0 893 1057 2754 Size: 893 164 1697 Fragments arranged by size: 1697 893 164 NdeI CA'TA_TG Cuts at: 0 2420 2754 Size: 2420 334 NspI r_CATG'y Cuts at: 0 290 2754 Size: 290 2464 PasI CC'CwG_GG Cuts at: 0 2487 2754 Size: 2487 267 PfoI T'CCnGG_A Cuts at: 0 482 2754 Size: 482 2272 PmlI CAC'GTG Cuts at: 0 867 2754 Size: 867 1887 PspOMI G'GGCC_C Cuts at: 0 1514 2754 Size: 1514 1240 PstI C_TGCA'G Cuts at: 0 908 1040 2754 Size: 908 132 1714 Fragments arranged by size: 1714 908 132 PvuII CAG'CTG Cuts at: 0 53 297 612 1020 1769 2754 Size: 53 244 315 408 749 985 Fragments arranged by size: 985 749 408 315 244 53 SacI G_AGCT'C Cuts at: 0 566 1899 2754 Size: 566 1333 855 Fragments arranged by size: 1333 855 566 ScaI AGT'ACT Cuts at: 0 107 754 2754 Size: 107 647 2000 Fragments arranged by size: 2000 647 107 SfcI C'TryA_G Cuts at: 0 807 904 1036 2754 Size: 807 97 132 1718 Fragments arranged by size: 1718 807 132 97 SfoI GGC'GCC Cuts at: 0 1063 2754 Size: 1063 1691 SmaI CCC'GGG Cuts at: 0 30 2754 Size: 30 2724 SmlI C'TyrA_G Cuts at: 0 646 718 1149 2754 Size: 646 72 431 1605 Fragments arranged by size: 1605 646 431 72 SpeI A'CTAG_T Cuts at: 0 1491 2754 Size: 1491 1263 SrfI GCCC'GGGC Cuts at: 0 30 2754 Size: 30 2724 SspI AAT'ATT Cuts at: 0 591 2502 2754 Size: 591 1911 252 Fragments arranged by size: 1911 591 252 StyI C'CwwG_G Cuts at: 0 893 1057 1650 2754 Size: 893 164 593 1104 Fragments arranged by size: 1104 893 593 164 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 763 930 1838 2754 Size: 763 167 908 916 Fragments arranged by size: 916 908 763 167 TatI w'GTAC_w Cuts at: 0 105 752 983 1382 1773 2754 Size: 105 647 231 399 391 981 Fragments arranged by size: 981 647 399 391 231 105 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 273 305 856 888 997 1029 2754 Size: 273 32 551 32 109 32 1725 Fragments arranged by size: 1725 551 273 109 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 301 616 2754 Size: 301 315 2138 Fragments arranged by size: 2138 315 301 XmaI C'CCGG_G Cuts at: 0 28 2754 Size: 28 2726 Enzymes that do cut: AccI Acc65I AcuI AflII AflIII AleI AlwNI ApaI ApoI AseI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BclI BglI Bme1580I BmgBI BmrI BpmI BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrGI BstBI BstXI BstYI BtgI BtsI CspCI EaeI EciI EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I NarI NcoI NdeI NspI PasI PfoI PmlI PspOMI PstI PvuII SacI ScaI SfcI SfoI SmaI SmlI SpeI SrfI SspI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII AclI AfeI AgeI AhdI AlfI AloI ApaLI AscI AsiSI BaeI BbvCI BciVI BfrBI BglII BlpI BmtI BplI Bpu10I BsaI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstEII BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EagI EarI EcoNI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI MfeI MluI NaeI NgoMIV NheI NotI NruI NsiI PacI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI SnaBI SphI StuI SwaI Tth111I XbaI XhoI XmnI ZraI