################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AlwI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspI AvaI AvaII AviII AvrII BalI BamHI BbvCI BcgI BciVI BclI BcnI BfrI BlnI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BsoBI BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoT14I EcoT22I FbaI FokI FseI FspI HapII HincII HindII HindIII HpaI HpaII KpnI Ksp632I MamI MfeI MluI MluNI MroI MscI MslI MspI MunI NaeI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TaqI TfiI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AcsI 542 AAATTT [GA]^AATT[CT] AcyI 49 GGCGCC G[GA]^CG[CT]C AhdI 561 GACAAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 222 GTGCAC G^TGCAC ApaLI 222 GTGCAC G^TGCAC ApoI 542 AAATTT [GA]^AATT[CT] AspEI 561 GACAAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 222 GTGCAC G[AT]GC[AT]^C BanI 49 GGCGCC G^G[CT][GA]CC BanII 70 GAGCCC G[GA]GC[CT]^C BbeI 49 GGCGCC GGCGC^C BbrPI 37 CACGTG CAC^GTG BbsI 558 GAAGAC GAAGAC(2/6) BglI 121 GCCGAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 480 AGATCT A^GATCT BpmI 106 CTGGAG CTGGAG(16/14) BpuAI 558 GAAGAC GAAGAC(2/6) BsaAI 37 CACGTG [CT]AC^GT[GA] BsaHI 49 GGCGCC G[GA]^CG[CT]C BsgI 584 CTGCAC GTGCAG(16/14) BsiHKAI 222 GTGCAC G[AT]GC[AT]^C BsiYI 25 CCGTTCCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 25 CCGTTCCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 262 GAGAC GTCTC(1/5) BsmI 181 GCATTC GAATGC(1/-1) Bsp1407I 215 TGTACA T^GTACA BspLU11I 218 ACATGT A^CATGT BsrGI 215 TGTACA T^GTACA BstAPI 112 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 480 AGATCT [GA]^GATC[CT] DraII 201 AGGCCCT [GA]G^G[ACGT]CC[CT] Eam1105I 561 GACAAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 201 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoRV 229 GATATC GAT^ATC FauI 73 CCCGC CCCGC(4/6) HaeII 49 GGCGCC [GA]GCGC^[CT] HgaI 100 GACGC GACGC(5/10) Hin1I 49 GGCGCC G[GA]^CG[CT]C HinfI 381 GACTC G^A[ACGT]TC KasI 49 GGCGCC G^GCGCC MflI 480 AGATCT [GA]^GATC[CT] NarI 49 GGCGCC GG^CGCC NspI 218 ACATGT [GA]CATG^[CT] PleI 381 GACTC GAGTC(4/5) PmaCI 37 CACGTG CAC^GTG PmlI 37 CACGTG CAC^GTG SfoI 49 GGCGCC GGC^GCC SspBI 215 TGTACA T^GTACA Tsp509I 543 AATT ^AATT XhoII 480 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AluI 249 AGCT AG^CT 420 AGCT AlwNI 113 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG 161 CAGGGTCTG AocI 205 CCTCAGG CC^T[ACGT]AGG 238 CCTGAGG BfaI 187 CTAG C^TAG 259 CTAG BsrI 126 ACTGG ACTGG(1/-1) 295 CCAGT BssKI 147 CCTGG ^CC[ACGT]GG 471 CCTGG BstNI 147 CCTGG CC^[AT]GG 471 CCTGG Bsu36I 205 CCTCAGG CC^T[ACGT]AGG 238 CCTGAGG CfoI 50 GCGC GCG^C 103 GCGC Cfr13I 42 GGGCC G^G[ACGT]CC 202 GGCCC DdeI 206 CTCAG C^T[ACGT]AG 239 CTGAG DpnII 349 GATC ^GATC 481 GATC Eco81I 205 CCTCAGG CC^T[ACGT]AGG 238 CCTGAGG EcoRII 147 CCTGG ^CC[AT]GG 471 CCTGG HhaI 50 GCGC GCG^C 103 GCGC HinP1I 50 GCGC G^CGC 103 GCGC HphI 490 TCACC GGTGA(8/7) 632 TCACC KspI 45 CCGCGG CCGC^GG 74 CCGCGG MaeI 187 CTAG C^TAG 259 CTAG MboI 349 GATC ^GATC 481 GATC MseI 362 TTAA T^TAA 521 TTAA MvaI 147 CCTGG CC^[AT]GG 471 CCTGG NdeII 349 GATC ^GATC 481 GATC NlaIII 219 CATG CATG^ 453 CATG SacII 45 CCGCGG CCGC^GG 74 CCGCGG Sau3AI 349 GATC ^GATC 481 GATC Sau96I 42 GGGCC G^G[ACGT]CC 202 GGCCC ScrFI 147 CCTGG CC^[ACGT]GG 471 CCTGG SmlI 389 CTCAAG C^T[CT][GA]AG 625 CTCAAG Tru9I 362 TTAA T^TAA 521 TTAA Tsp45I 379 GTGAC ^GT[GC]AC 404 GTCAC TspRI 225 CACTG CA[GC]TG[ACGT][ACGT]^ 582 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AccII AciI AfaI AflIII BbvI BmyI BsaJI Bsp1286I BstUI Cac8I DsaI Fnu4HI HaeIII ItaI MaeII MaeIII MboII MnlI MspA1I MvnI MwoI NlaIV RsaI TseI