Restriction map for
pFN21AE1708
(Circular) (Six-base) MAPSORT of: pFN21AE1708.seq Check: 6178 from: 1 to: 5347
pFN21AE1708 5347 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3157 278
Size: 53 83 186 936 1621 2468
Fragments arranged by size:
2468 1621 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2690 1937
Size: 753 4594
Acc65I G'GTAC_C
Cuts at: 2668 2668
Size: 5347
AclI AA'CG_TT
Cuts at: 1563 3474 3847 1563
Size: 1911 373 3063
Fragments arranged by size:
3063 1911 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2044 3410 4794 5062 1632
Size: 412 1366 1384 268 1917
Fragments arranged by size:
1917 1384 1366 412 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5150
AflIII A'CryG_T
Cuts at: 1203 2067 2088 2223 4246 5223 1203
Size: 864 21 135 2023 977 1327
Fragments arranged by size:
2023 1327 977 864 135 21
AgeI A'CCGG_T
Cuts at: 4178 4178
Size: 5347
AhdI GACnn_n'nnGTC
Cuts at: 2571 4076 2571
Size: 1505 3842
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5347
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5347
AlwNI CAG_nnn'CTG
Cuts at: 2123 2171 3090 4662 2123
Size: 48 919 1572 2808
Fragments arranged by size:
2808 1572 919 48
ApaLI G'TGCA_C
Cuts at: 2227 3404 4560 2227
Size: 1177 1156 3014
Fragments arranged by size:
3014 1177 1156
ApoI r'AATT_y
Cuts at: 1493 2547 2656 2882 3034 5143 1493
Size: 1054 109 226 152 2109 1697
Fragments arranged by size:
2109 1697 1054 226 152 109
AseI AT'TA_AT
Cuts at: 160 1032 3901 160
Size: 872 2869 1606
Fragments arranged by size:
2869 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5347
AvaI C'yCGr_G
Cuts at: 1870 1943 2672 1870
Size: 73 729 4545
Fragments arranged by size:
4545 729 73
BamHI G'GATC_C
Cuts at: 2677 3044 2677
Size: 367 4980
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2054 2668 4123 618
Size: 325 217 627 267 614 1455 1842
Fragments arranged by size:
1842 1455 627 614 325 267 217
BanII G_rGCy'C
Cuts at: 1391 2079 2666 1391
Size: 688 587 4072
Fragments arranged by size:
4072 688 587
BbeI G_GCGC'C
Cuts at: 2058 2058
Size: 5347
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2570 928
Size: 971 671 3705
Fragments arranged by size:
3705 971 671
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3538 3572 1903
Size: 34 1601 34 3678
Fragments arranged by size:
3678 1601 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3239 4449 3239
Size: 1210 4137
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5347
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2132 3958 136
Size: 107 122 71 631 605 460 1826 1525
Fragments arranged by size:
1826 1525 631 605 460 122 107 71
BglII A'GATC_T
Cuts at: 2485 5342 2485
Size: 2857 2490
BlpI GC'TnA_GC
Cuts at: 2764 2764
Size: 5347
Bme1580I G_kGCm'C
Cuts at: 1790 2043 2231 3408 4564 1790
Size: 253 188 1177 1156 2573
Fragments arranged by size:
2573 1177 1156 253 188
BmgBI CAC'GTC
Cuts at: 1318 2068 1318
Size: 750 4597
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3078 4036 442
Size: 416 53 83 399 24 1661 958 1753
Fragments arranged by size:
1753 1661 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5347
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 4007 1239
Size: 893 1875 2579
Fragments arranged by size:
2579 1875 893
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2379 2615 3472 4337 4635 2379
Size: 236 857 865 298 3091
Fragments arranged by size:
3091 865 857 298 236
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4010 882
Size: 617 2511 2219
Fragments arranged by size:
2511 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2044 3138 493
Size: 711 840 1094 2702
Fragments arranged by size:
2702 1094 840 711
BsaBI GATnn'nnATC
Cuts at: 5341 5341
Size: 5347
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2055 3154 3536 4171
Size: 53 83 186 936 522 1099 382 635
Cuts at: 4171 275
Size: 1451
Fragments arranged by size:
1451 1099 936 635 522 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3780 4178 4452 4599 5010 3780
Size: 398 274 147 411 4117
Fragments arranged by size:
4117 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2075 2105 1178
Size: 30 165 30 672 30 4420
Fragments arranged by size:
4420 672 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5347
BseYI C'CCAG_C
Cuts at: 4550 5046 4550
Size: 496 4851
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2574 1163
Size: 573 838 3936
Fragments arranged by size:
3936 838 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2797 3558 4586 1540
Size: 275 186 796 761 1028 2301
Fragments arranged by size:
2301 1028 796 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2231 3408 3493 4564 2231
Size: 1177 85 1071 3014
Fragments arranged by size:
3014 1177 1071 85
BsmI GAATG_Cn'
Cuts at: 2186 2858 2951 2186
Size: 672 93 4582
Fragments arranged by size:
4582 672 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2079 2231 2666 3408 3493 4564
Size: 399 253 36 152 435 742 85 1071
Cuts at: 4564 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 435 399 253 152 85
36
BspEI T'CCGG_A
Cuts at: 5010 5010
Size: 5347
BspHI T'CATG_A
Cuts at: 3236 4966 3236
Size: 1730 3617
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2702 844
Size: 1858 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3234 1394
Size: 429 1411 3507
Fragments arranged by size:
3507 1411 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3842 4016 65
Size: 1183 2594 174 1396
Fragments arranged by size:
2594 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3991 4178 1700
Size: 110 2181 187 2869
Fragments arranged by size:
2869 2181 187 110
BsrGI T'GTAC_A
Cuts at: 96 2220 96
Size: 2124 3223
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5347
BssSI C'ACGA_G
Cuts at: 3407 4419 3407
Size: 1012 4335
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2123 794
Size: 1329 4018
BstBI TT'CG_AA
Cuts at: 4151 4151
Size: 5347
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5347
BstYI r'GATC_y
Cuts at: 1972 2485 2677 3044 3429 3446 4898 4984 4996
Size: 513 192 367 385 17 1452 86 12
Cuts at: 4996 5342 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 513 385 367 346 192 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2211 2244 2211
Size: 33 5314
BtgI C'CryG_G
Cuts at: 513 1065 2004 2035 2050 2079 513
Size: 552 939 31 15 29 3781
Fragments arranged by size:
3781 939 552 31 29 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4608
Fragments arranged by size:
4608 683 56
BtsI GCAGTG_nn'
Cuts at: 2586 2863 3657 3677 2586
Size: 277 794 20 4256
Fragments arranged by size:
4256 794 277 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5347
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5312
DraI TTT'AAA
Cuts at: 2651 2651
Size: 5347
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4354 809
Size: 507 3038 1802
Fragments arranged by size:
3038 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2794 3683 5312
Size: 54 1425 50 275 18 964 889 1629
Cuts at: 5312 8
Size: 43
Fragments arranged by size:
1629 1425 964 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2794 1537
Size: 275 982 4090
Fragments arranged by size:
4090 982 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3277 1353
Size: 1924 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3934 4308 4454 387
Size: 1064 138 2345 374 146 1280
Fragments arranged by size:
2345 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2044 2132 3410 4007 4794 5062 1239
Size: 393 412 88 1278 597 787 268 1524
Fragments arranged by size:
1524 1278 787 597 412 393 268 88
EcoO109I rG'GnC_Cy
Cuts at: 2207 2207
Size: 5347
EcoRI G'AATT_C
Cuts at: 2656 3034 2656
Size: 378 4969
EcoRV GAT'ATC
Cuts at: 2236 2236
Size: 5347
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2372 2636 2372
Size: 264 5083
FspI TGC'GCA
Cuts at: 3853 3853
Size: 5347
HaeII r_GCGC'y
Cuts at: 1678 2058 4494 1678
Size: 380 2436 2531
Fragments arranged by size:
2531 2436 380
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2194 2226 2377 2409 2557
Size: 32 135 32 622 32 151 32 148
Cuts at: 2557 2589 3988 4020 4062 4094 1373
Size: 32 1399 32 42 32 2626
Fragments arranged by size:
2626 1399 622 151 148 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2691 2937 669
Size: 988 281 753 246 3079
Fragments arranged by size:
3079 988 753 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5347
HpaI GTT'AAC
Cuts at: 2937 2937
Size: 5347
KasI G'GCGC_C
Cuts at: 2054 2054
Size: 5347
KpnI G_GTAC'C
Cuts at: 2672 2672
Size: 5347
MfeI C'AATT_G
Cuts at: 2946 2946
Size: 5347
MluI A'CGCG_T
Cuts at: 5223 5223
Size: 5347
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4461 4645 1305
Size: 3156 184 2007
Fragments arranged by size:
3156 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3525
Fragments arranged by size:
3525 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3305 3664 3823 518
Size: 648 2139 359 159 2042
Fragments arranged by size:
2139 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2052 2081 2120 2763 3440 4588 4833 1606
Size: 446 29 39 643 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 643 446 245 39 29
NarI GG'CG_CC
Cuts at: 2055 2055
Size: 5347
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3856
Fragments arranged by size:
3856 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5347
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5347
NotI GC'GGCC_GC
Cuts at: 2794 2794
Size: 5347
NspI r_CATG'y
Cuts at: 1132 1229 2227 2705 4250 1132
Size: 97 998 478 1545 2229
Fragments arranged by size:
2229 1545 998 478 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5347
PciI A'CATG_T
Cuts at: 2223 4246 2223
Size: 2023 3324
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5347
PmeI GTTT'AAAC
Cuts at: 2651 2651
Size: 5347
PmlI CAC'GTG
Cuts at: 2044 2044
Size: 5347
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3416 3448 4929 4961 3416
Size: 32 1481 32 3802
Fragments arranged by size:
3802 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5347
PsiI TTA'TAA
Cuts at: 2917 2917
Size: 5347
PstI C_TGCA'G
Cuts at: 830 2699 830
Size: 1869 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5347
SacII CC_GC'GG
Cuts at: 2053 2082 2053
Size: 29 5318
SalI G'TCGA_C
Cuts at: 1936 2689 1936
Size: 753 4594
SbfI CC_TGCA'GG
Cuts at: 2699 2699
Size: 5347
ScaI AGT'ACT
Cuts at: 3595 5307 3595
Size: 1712 3635
SfcI C'TryA_G
Cuts at: 826 1045 2695 3830 4511 4702 826
Size: 219 1650 1135 681 191 1471
Fragments arranged by size:
1650 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2056 2056
Size: 5347
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5347
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5347
SmaI CCC'GGG
Cuts at: 1872 2674 1872
Size: 802 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2394 2630 3451 4352 4614 820
Size: 197 926 451 236 821 901 262 1553
Fragments arranged by size:
1553 926 901 821 451 262 236 197
SnaBI TAC'GTA
Cuts at: 493 3138 493
Size: 2645 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5347
SphI G_CATG'C
Cuts at: 1132 2705 1132
Size: 1573 3774
SspI AAT'ATT
Cuts at: 5 52 3271 5
Size: 47 3219 2081
Fragments arranged by size:
3219 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5347
StyI C'CwwG_G
Cuts at: 513 1065 2004 2786 513
Size: 552 939 782 3074
Fragments arranged by size:
3074 939 782 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2357 3374 3391 3544 3729 4174 325
Size: 1255 777 1017 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1017 777 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2220 3593 5305
Size: 274 80 33 51 1443 243 1373 1712
Cuts at: 5305 96
Size: 138
Fragments arranged by size:
1712 1443 1373 274 243 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5307 5339 5307
Size: 32 5315
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5347
XbaI T'CTAG_A
Cuts at: 2683 2683
Size: 5347
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5347
XmaI C'CCGG_G
Cuts at: 1870 2672 1870
Size: 802 4545
XmnI GAAnn'nnTTC
Cuts at: 3476 3476
Size: 5347
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3155 276
Size: 53 83 186 936 1621 2468
Fragments arranged by size:
2468 1621 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PstI PvuI SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI