################################## # NO CUT ENZYMES ( 86 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI Aor51HI AscI AseI AsnI Asp718I AviII AvrII BbeI BbrPI BcgI BciVI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaWI BseAI BsiWI BsmI BspDI BspEI BspHI BspLU11I BsrBI BssHII Bst1107I BstBI BstZ17I CelII ClaI DraI DraIII Eco47III EcoRI EcoRV FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MroI NarI NcoI NdeI NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccI 382 GTCTAC GT^[AC][GT]AC AcyI 259 GACGCC G[GA]^CG[CT]C AflIII 966 ACGCGT A^C[GA][CT]GT AhdI 511 GACGCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 694 GTGCAC G^TGCAC AocI 1500 CCTAAGG CC^T[ACGT]AGG ApaLI 694 GTGCAC G^TGCAC Asp700I 2807 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 511 GACGCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 2699 TGGCCA TGG^CCA BamHI 1681 GGATCC G^GATCC BclI 267 TGATCA T^GATCA BsaBI 976 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 259 GACGCC G[GA]^CG[CT]C BsaI 2936 GGTCTC GGTCTC(1/5) BsiEI 985 CGGCCG CG[GA][CT]^CG BsmBI 555 CGTCTC CGTCTC(1/5) Bsp1407I 1357 TGTACA T^GTACA BspMI 2615 ACCTGC ACCTGC(4/8) BsrGI 1357 TGTACA T^GTACA BssSI 756 CACGAG CACGAG(-5/-1) Bsu36I 1500 CCTAAGG CC^T[ACGT]AGG CpoI 550 CGGTCCG CG^G[AT]CCG DsaI 2126 CCACGG C^C[GA][CT]GG EagI 985 CGGCCG C^GGCCG Eam1105I 511 GACGCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 985 CGGCCG C^GGCCG Eco52I 985 CGGCCG C^GGCCG Eco81I 1500 CCTAAGG CC^T[ACGT]AGG EcoT14I 426 CCTTGG C^C[AT][AT]GG EcoT22I 2070 ATGCAT ATGCA^T FbaI 267 TGATCA T^GATCA Hin1I 259 GACGCC G[GA]^CG[CT]C MamI 976 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 357 CAATTG C^AATTG MluI 966 ACGCGT A^CGCGT MluNI 2699 TGGCCA TGG^CCA MscI 2699 TGGCCA TGG^CCA MunI 357 CAATTG C^AATTG NaeI 2528 GCCGGC GCC^GGC NgoMIV 2528 GCCGGC G^CCGGC NsiI 2070 ATGCAT ATGCA^T NspI 3018 ACATGC [GA]CATG^[CT] PaeR7I 2583 CTCGAG C^TCGAG PshAI 375 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsrII 550 CGGTCCG CG^G[AT]CCG SacI 1158 GAGCTC GAGCT^C SmaI 2915 CCCGGG CCC^GGG SspBI 1357 TGTACA T^GTACA StyI 426 CCTTGG C^C[AT][AT]GG XcmI 1187 CCACCCACATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 2583 CTCGAG C^TCGAG XmaCI 2915 CCCGGG C^CCGGG XmaI 2915 CCCGGG C^CCGGG XmnI 2807 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccII 663 CGCG CG^CG 967 CGCG AcsI 2740 AAATTT [GA]^AATT[CT] 2977 AAATTC ApoI 2740 AAATTT [GA]^AATT[CT] 2977 AAATTC AspHI 694 GTGCAC G[AT]GC[AT]^C 1158 GAGCTC AspI 2301 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2389 GACATGGTC AvaI 2583 CTCGAG C^[CT]CG[GA]G 2915 CCCGGG BbsI 1510 GAAGAC GAAGAC(2/6) 1813 GAAGAC BbvCI 2548 CCTCAGC CCTCAGC(-5/-2) 1722 GCTGAGG BfaI 160 CTAG C^TAG 679 CTAG BglII 1133 AGATCT A^GATCT 2909 AGATCT BpuAI 1510 GAAGAC GAAGAC(2/6) 1813 GAAGAC BsgI 583 GTGCAG GTGCAG(16/14) 657 GTGCAG BsiHKAI 694 GTGCAC G[AT]GC[AT]^C 1158 GAGCTC BsoBI 2583 CTCGAG C^[CT]CG[GA]G 2915 CCCGGG BsrFI 2217 GCCGGT [GA]^CCGG[CT] 2528 GCCGGC BstEII 948 GGTCACC G^GT[ACGT]ACC 1221 GGTCACC BstPI 948 GGTCACC G^GT[ACGT]ACC 1221 GGTCACC BstUI 663 CGCG CG^CG 967 CGCG BstXI 1772 CCACACACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2453 CCATGAAGCTGG Cfr10I 2217 GCCGGT [GA]^CCGG[CT] 2528 GCCGGC EaeI 985 CGGCCG [CT]^GGCC[GA] 2699 TGGCCA EcoO65I 948 GGTCACC G^GT[ACGT]ACC 1221 GGTCACC MaeI 160 CTAG C^TAG 679 CTAG MvnI 663 CGCG CG^CG 967 CGCG PflFI 2301 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2389 GACATGGTC PpuMI 1944 GGGTCCC [GA]G^G[AT]CC[CT] 2444 GGGTCCC Tth111I 2301 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2389 GACATGGTC ######################################### # 3 OR MORE CUT ENZYMES ( 90 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI ApaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DrdI EarI EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII