(Linear) (Six-base) MAPSORT of: KIEE3349.seq Check: 4245 from: 1 to: 3102 KIEE3349.orf With 182 enzymes: SgfI * December 5, 2014 12:56 .. AccI GT'mk_AC Cuts at: 0 383 3102 Size: 383 2719 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 174 1972 3102 Size: 174 1798 1130 Fragments arranged by size: 1798 1130 174 AflIII A'CryG_T Cuts at: 0 966 3102 Size: 966 2136 AhdI GACnn_n'nnGTC Cuts at: 0 516 3102 Size: 516 2586 AleI CACnn'nnGTG Cuts at: 0 1740 3102 Size: 1740 1362 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 375 2632 3102 Size: 375 2257 470 Fragments arranged by size: 2257 470 375 AlwNI CAG_nnn'CTG Cuts at: 0 1692 2007 2693 2789 3102 Size: 1692 315 686 96 313 Fragments arranged by size: 1692 686 315 313 96 ApaI G_GGCC'C Cuts at: 0 172 1605 2635 3102 Size: 172 1433 1030 467 Fragments arranged by size: 1433 1030 467 172 ApaLI G'TGCA_C Cuts at: 0 694 3102 Size: 694 2408 ApoI r'AATT_y Cuts at: 0 2740 2977 3102 Size: 2740 237 125 Fragments arranged by size: 2740 237 125 AvaI C'yCGr_G Cuts at: 0 2583 2915 3102 Size: 2583 332 187 Fragments arranged by size: 2583 332 187 BamHI G'GATC_C Cuts at: 0 1681 3102 Size: 1681 1421 BanI G'GyrC_C Cuts at: 0 21 503 1657 3102 Size: 21 482 1154 1445 Fragments arranged by size: 1445 1154 482 21 BanII G_rGCy'C Cuts at: 0 172 1162 1605 2015 2135 2635 3102 Size: 172 990 443 410 120 500 467 Fragments arranged by size: 990 500 467 443 410 172 120 BbsI GAAGACnn'nnnn_ Cuts at: 0 1517 1820 3102 Size: 1517 303 1282 Fragments arranged by size: 1517 1282 303 BbvCI CC'TCA_GC Cuts at: 0 1723 2549 3102 Size: 1723 826 553 Fragments arranged by size: 1723 826 553 BclI T'GATC_A Cuts at: 0 267 3102 Size: 267 2835 BfrBI ATG'CAT Cuts at: 0 2072 3102 Size: 2072 1030 BglII A'GATC_T Cuts at: 0 1133 2909 3102 Size: 1133 1776 193 Fragments arranged by size: 1776 1133 193 Bme1580I G_kGCm'C Cuts at: 0 172 698 1605 1660 2635 3102 Size: 172 526 907 55 975 467 Fragments arranged by size: 975 907 526 467 172 55 BmgBI CAC'GTC Cuts at: 0 333 3090 3102 Size: 333 2757 12 Fragments arranged by size: 2757 333 12 BmrI ACTGGGnnnn_n' Cuts at: 0 2568 2793 3102 Size: 2568 225 309 Fragments arranged by size: 2568 309 225 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2508 3102 Size: 2508 594 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 191 1632 1904 2029 2139 2424 2478 2924 Size: 191 1441 272 125 110 285 54 446 Cuts at: 2924 3102 Size: 178 Fragments arranged by size: 1441 446 285 272 191 178 125 110 54 Bpu10I CC'TnA_GC Cuts at: 0 1723 2549 2719 3037 3102 Size: 1723 826 170 318 65 Fragments arranged by size: 1723 826 318 170 65 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1201 1778 2244 3102 Size: 1201 577 466 858 Fragments arranged by size: 1201 858 577 466 BsaI GGTCTCn'nnnn_ Cuts at: 0 2942 3102 Size: 2942 160 BsaBI GATnn'nnATC Cuts at: 0 980 3102 Size: 980 2122 BsaHI Gr'CG_yC Cuts at: 0 260 3102 Size: 260 2842 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 447 477 2779 2809 3102 Size: 447 30 2302 30 293 Fragments arranged by size: 2302 447 293 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 21 85 103 106 109 112 169 468 Size: 21 64 18 3 3 3 57 299 Cuts at: 468 1972 1975 2424 2427 3102 Size: 1504 3 449 3 675 Fragments arranged by size: 1504 675 449 299 64 57 21 18 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 565 1606 1624 1941 2000 3102 Size: 565 1041 18 317 59 1102 Fragments arranged by size: 1102 1041 565 317 59 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 604 678 3102 Size: 604 74 2424 Fragments arranged by size: 2424 604 74 BsiEI CG_ry'CG Cuts at: 0 988 3102 Size: 988 2114 BsiHKAI G_wGCw'C Cuts at: 0 698 1162 3102 Size: 698 464 1940 Fragments arranged by size: 1940 698 464 BsmBI CGTCTCn'nnnn_ Cuts at: 0 561 3102 Size: 561 2541 Bsp1286I G_dGCh'C Cuts at: 0 172 698 1162 1605 1660 2015 2135 2635 Size: 172 526 464 443 55 355 120 500 Cuts at: 2635 3102 Size: 467 Fragments arranged by size: 526 500 467 464 443 355 172 120 55 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2624 3102 Size: 2624 478 BsrDI GCAATG_nn' Cuts at: 0 141 1730 2037 3102 Size: 141 1589 307 1065 Fragments arranged by size: 1589 1065 307 141 BsrFI r'CCGG_y Cuts at: 0 2217 2528 3102 Size: 2217 311 574 Fragments arranged by size: 2217 574 311 BsrGI T'GTAC_A Cuts at: 0 1357 3102 Size: 1357 1745 BssSI C'ACGA_G Cuts at: 0 756 3102 Size: 756 2346 BstAPI GCAn_nnn'nTGC Cuts at: 0 540 611 2036 3102 Size: 540 71 1425 1066 Fragments arranged by size: 1425 1066 540 71 BstEII G'GTnAC_C Cuts at: 0 948 1221 3102 Size: 948 273 1881 Fragments arranged by size: 1881 948 273 BstXI CCAn_nnnn'nTGG Cuts at: 0 1779 2460 3102 Size: 1779 681 642 Fragments arranged by size: 1779 681 642 BstYI r'GATC_y Cuts at: 0 1012 1133 1455 1681 1697 2909 3102 Size: 1012 121 322 226 16 1212 193 Fragments arranged by size: 1212 1012 322 226 193 121 16 Bsu36I CC'TnA_GG Cuts at: 0 1501 3102 Size: 1501 1601 BtgI C'CryG_G Cuts at: 0 2126 3102 Size: 2126 976 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2367 3102 Size: 2367 735 BtsI GCAGTG_nn' Cuts at: 0 1280 2790 3102 Size: 1280 1510 312 Fragments arranged by size: 1510 1280 312 DrdI GACnn_nn'nnGTC Cuts at: 0 679 2218 2874 3102 Size: 679 1539 656 228 Fragments arranged by size: 1539 679 656 228 EaeI y'GGCC_r Cuts at: 0 985 2699 3102 Size: 985 1714 403 Fragments arranged by size: 1714 985 403 EagI C'GGCC_G Cuts at: 0 985 3102 Size: 985 2117 EarI CTCTTCn'nnn_ Cuts at: 0 62 80 1856 2596 2954 3102 Size: 62 18 1776 740 358 148 Fragments arranged by size: 1776 740 358 148 62 18 EcoICRI GAG'CTC Cuts at: 0 1160 3102 Size: 1160 1942 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 174 191 1632 1904 1972 2029 2139 2424 Size: 174 17 1441 272 68 57 110 285 Cuts at: 2424 2478 2924 3102 Size: 54 446 178 Fragments arranged by size: 1441 446 285 272 178 174 110 68 57 54 17 EcoNI CCTnn'n_nnAGG Cuts at: 0 1504 1954 2238 2439 3102 Size: 1504 450 284 201 663 Fragments arranged by size: 1504 663 450 284 201 EcoO109I rG'GnC_Cy Cuts at: 0 1302 1601 1602 1945 2445 3102 Size: 1302 299 1 343 500 657 Fragments arranged by size: 1302 657 500 343 299 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2238 3102 Size: 2238 864 HaeII r_GCGC'y Cuts at: 0 2090 2265 2481 3102 Size: 2090 175 216 621 Fragments arranged by size: 2090 621 216 175 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 229 259 261 291 502 534 1855 1887 Size: 229 30 2 30 211 32 1321 32 Cuts at: 1887 2002 2034 2779 2811 2956 2988 3102 Size: 115 32 745 32 145 32 114 Fragments arranged by size: 1321 745 229 211 145 115 114 32 32 32 32 32 30 30 2 MfeI C'AATT_G Cuts at: 0 357 3102 Size: 357 2745 MluI A'CGCG_T Cuts at: 0 966 3102 Size: 966 2136 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 257 380 1091 2561 2980 3102 Size: 257 123 711 1470 419 122 Fragments arranged by size: 1470 711 419 257 123 122 MscI TGG'CCA Cuts at: 0 2701 3102 Size: 2701 401 MslI CAynn'nnrTG Cuts at: 0 701 1192 1740 3102 Size: 701 491 548 1362 Fragments arranged by size: 1362 701 548 491 MspA1I CmG'CkG Cuts at: 0 60 107 379 1254 1610 1647 3102 Size: 60 47 272 875 356 37 1455 Fragments arranged by size: 1455 875 356 272 60 47 37 NaeI GCC'GGC Cuts at: 0 2530 3102 Size: 2530 572 NgoMIV G'CCGG_C Cuts at: 0 2528 3102 Size: 2528 574 NsiI A_TGCA'T Cuts at: 0 2074 3102 Size: 2074 1028 NspI r_CATG'y Cuts at: 0 3022 3102 Size: 3022 80 PflMI CCAn_nnn'nTGG Cuts at: 0 795 1197 2007 3102 Size: 795 402 810 1095 Fragments arranged by size: 1095 810 795 402 PfoI T'CCnGG_A Cuts at: 0 207 788 3102 Size: 207 581 2314 Fragments arranged by size: 2314 581 207 PpuMI rG'GwC_Cy Cuts at: 0 1945 2445 3102 Size: 1945 500 657 Fragments arranged by size: 1945 657 500 PshAI GACnn'nnGTC Cuts at: 0 379 3102 Size: 379 2723 PspOMI G'GGCC_C Cuts at: 0 168 1601 2631 3102 Size: 168 1433 1030 471 Fragments arranged by size: 1433 1030 471 168 PspXI vC'TCGA_Gb Cuts at: 0 2583 3102 Size: 2583 519 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 2799 2831 3102 Size: 2799 32 271 Fragments arranged by size: 2799 271 32 PstI C_TGCA'G Cuts at: 0 128 533 1697 2096 3102 Size: 128 405 1164 399 1006 Fragments arranged by size: 1164 1006 405 399 128 PvuII CAG'CTG Cuts at: 0 60 1254 1610 1647 3102 Size: 60 1194 356 37 1455 Fragments arranged by size: 1455 1194 356 60 37 RsrII CG'GwC_CG Cuts at: 0 551 3102 Size: 551 2551 SacI G_AGCT'C Cuts at: 0 1162 3102 Size: 1162 1940 SanDI GG'GwC_CC Cuts at: 0 1945 2445 3102 Size: 1945 500 657 Fragments arranged by size: 1945 657 500 SfcI C'TryA_G Cuts at: 0 124 529 1693 2092 3102 Size: 124 405 1164 399 1010 Fragments arranged by size: 1164 1010 405 399 124 SmaI CCC'GGG Cuts at: 0 2917 3102 Size: 2917 185 SmlI C'TyrA_G Cuts at: 0 1216 1793 2223 2583 3102 Size: 1216 577 430 360 519 Fragments arranged by size: 1216 577 519 430 360 StuI AGG'CCT Cuts at: 0 419 1120 1867 3102 Size: 419 701 747 1235 Fragments arranged by size: 1235 747 701 419 StyI C'CwwG_G Cuts at: 0 426 3102 Size: 426 2676 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 39 539 1204 1951 2024 3102 Size: 39 500 665 747 73 1078 Fragments arranged by size: 1078 747 665 500 73 39 TatI w'GTAC_w Cuts at: 0 1357 3102 Size: 1357 1745 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 447 479 3102 Size: 447 32 2623 Fragments arranged by size: 2623 447 32 Tth111I GACn'n_nGTC Cuts at: 0 2304 2392 3102 Size: 2304 88 710 Fragments arranged by size: 2304 710 88 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1194 3102 Size: 1194 1908 XhoI C'TCGA_G Cuts at: 0 2583 3102 Size: 2583 519 XmaI C'CCGG_G Cuts at: 0 2915 3102 Size: 2915 187 XmnI GAAnn'nnTTC Cuts at: 0 2811 3102 Size: 2811 291 Enzymes that do cut: AccI AcuI AflIII AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI BclI BfrBI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I MfeI MluI MmeI MscI MslI MspA1I NaeI NgoMIV NsiI NspI PflMI PfoI PpuMI PshAI PspOMI PspXI PsrI PstI PvuII RsrII SacI SanDI SfcI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AloI AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI BglI BlpI BmtI BsaAI BsaWI BsiWI BsmI BspEI BspHI BsrBI BssHII BstBI BstZ17I ClaI CspCI DraI DraIII EciI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI KpnI NarI NcoI NdeI NheI NotI NruI PacI PasI PciI PmeI PmlI PpiI PsiI PvuI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI ZraI