Restriction map for
pFN21AE2326
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# NO CUT ENZYMES ( 111 enzymes) #
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Acc65I AccI AclI AcsI AflII
AflIII AgeI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BbrPI BclI BfrI BglII
BlnI BlpI Bpu1102I BsgI BsiWI
BsmI Bsp1407I BspDI BspLU11I BspMI
BsrFI BsrGI BssHII BssSI Bst1107I
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DsaI EarI
Eco47III Eco81I EcoRI EcoRV EcoT22I
FbaI FseI FspI HpaI KpnI
Ksp632I KspI MfeI MluI MseI
MspA1I MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tsp509I Tth111I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 1315 GACGTC GACGT^C
AhdI 1315 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1315 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 54 GGCGCC GGCGC^C
BbvCI 288 CCTCAGC CCTCAGC(-5/-2)
BcgI 389 GCAACTATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 322 CTAG C^TAG
BglI 901 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 151 TACGTG [CT]AC^GT[GA]
BsaBI 1341 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 41 GAGACG CGTCTC(1/5)
BsmFI 305 GGGAC GGGAC(10/14)
BsrDI 1061 CATTGC GCAATG(2/0)
BsrI 1560 CCAGT ACTGG(1/-1)
BstAPI 1056 GCAGCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1510 TTCGAA TT^CGAA
BstEII 969 GGTCACC G^GT[ACGT]ACC
BstPI 969 GGTCACC G^GT[ACGT]ACC
BstXI 1154 CCAACATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 183 GGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 943 GACAAGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1315 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 183 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 969 GGTCACC G^GT[ACGT]ACC
HgaI 457 GACGC GACGC(5/10)
HincII 406 GTTGAC GT[CT]^[GA]AC
HindII 406 GTTGAC GT[CT]^[GA]AC
HindIII 1017 AAGCTT A^AGCTT
KasI 54 GGCGCC G^GCGCC
MaeI 322 CTAG C^TAG
MamI 1341 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 54 GGCGCC GG^CGCC
NspV 1510 TTCGAA TT^CGAA
PleI 409 GACTC GAGTC(4/5)
SfoI 54 GGCGCC GGC^GCC
SfuI 1510 TTCGAA TT^CGAA
SmaI 1208 CCCGGG CCC^GGG
StuI 899 AGGCCT AGG^CCT
XmaCI 1208 CCCGGG C^CCGGG
XmaI 1208 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AccII 38 CGCG CG^CG
403 CGCG
AccIII 599 TCCGGA T^CCGGA
1491 TCCGGA
AcyI 54 GGCGCC G[GA]^CG[CT]C
1315 GACGTC
AspHI 1403 GAGCTC G[AT]GC[AT]^C
1453 GAGCTC
BanI 54 GGCGCC G^G[CT][GA]CC
714 GGTGCC
BciVI 1417 GGATAC GTATCC(6/5)
1331 GTATCC
BsaHI 54 GGCGCC G[GA]^CG[CT]C
1315 GACGTC
BseAI 599 TCCGGA T^CCGGA
1491 TCCGGA
BsiEI 1359 CGGCCG CG[GA][CT]^CG
1501 CGGCCG
BsiHKAI 1403 GAGCTC G[AT]GC[AT]^C
1453 GAGCTC
BspEI 599 TCCGGA T^CCGGA
1491 TCCGGA
BspHI 503 TCATGA T^CATGA
1466 TCATGA
BsrBI 49 GAGCGG CCGCTC(-3/-3)
1498 GAGCGG
BstUI 38 CGCG CG^CG
403 CGCG
EagI 1359 CGGCCG C^GGCCG
1501 CGGCCG
EclXI 1359 CGGCCG C^GGCCG
1501 CGGCCG
Eco52I 1359 CGGCCG C^GGCCG
1501 CGGCCG
EcoNI 906 CCTGGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1369 CCTTACCCAGG
Hin1I 54 GGCGCC G[GA]^CG[CT]C
1315 GACGTC
MaeIII 970 GTCAC ^GT[ACGT]AC
1351 GTCAC
MroI 599 TCCGGA T^CCGGA
1491 TCCGGA
MvnI 38 CGCG CG^CG
403 CGCG
PstI 655 CTGCAG CTGCA^G
934 CTGCAG
RcaI 503 TCATGA T^CATGA
1466 TCATGA
SacI 1403 GAGCTC GAGCT^C
1453 GAGCTC
SfcI 655 CTGCAG C^T[GA][CT]AG
934 CTGCAG
SmlI 12 CTCAAG C^T[CT][GA]AG
784 CTCAAG
Tsp45I 970 GTCAC ^GT[GC]AC
1351 GTCAC
TspRI 139 CACTG CA[GC]TG[ACGT][ACGT]^
863 CAGTG
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# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaI AvaII BalI BamHI BanII
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaI BsaJI BsaWI BseRI
BsiYI BslI BsmAI BsoBI Bsp1286I
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MboI MboII MflI MluNI MnlI
MscI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI TfiI TseI
TthHB8I Van91I XhoII