(Circular) (Six-base) MAPSORT of: pFN21AE2326.seq Check: 2348 from: 1 to: 6283 pFN21AE2326 6283 bp With 182 enzymes: SgfI * March 28, 2012 14:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3324 4093 278 Size: 53 83 186 936 1788 769 2468 Fragments arranged by size: 2468 1788 936 769 186 83 53 AccI GT'mk_AC Cuts at: 1937 3626 1937 Size: 1689 4594 Acc65I G'GTAC_C Cuts at: 3604 3604 Size: 6283 AclI AA'CG_TT Cuts at: 1563 4410 4783 1563 Size: 2847 373 3063 Fragments arranged by size: 3063 2847 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2635 3303 3417 4346 5730 5998 1632 Size: 1003 668 114 929 1384 268 1917 Fragments arranged by size: 1917 1384 1003 929 668 268 114 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6086 AflIII A'CryG_T Cuts at: 1203 5182 6159 1203 Size: 3979 977 1327 Fragments arranged by size: 3979 1327 977 AgeI A'CCGG_T Cuts at: 5114 5114 Size: 6283 AhdI GACnn_n'nnGTC Cuts at: 3325 5012 3325 Size: 1687 4596 AleI CACnn'nnGTG Cuts at: 1166 2861 1166 Size: 1695 4588 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6283 AlwNI CAG_nnn'CTG Cuts at: 2237 2668 2713 3281 4026 5598 2237 Size: 431 45 568 745 1572 2922 Fragments arranged by size: 2922 1572 745 568 431 45 ApaLI G'TGCA_C Cuts at: 4340 5496 4340 Size: 1156 5127 ApoI r'AATT_y Cuts at: 1493 3592 3818 3970 6079 1493 Size: 2099 226 152 2109 1697 Fragments arranged by size: 2109 2099 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4837 160 Size: 872 3805 1606 Fragments arranged by size: 3805 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6283 AvaI C'yCGr_G Cuts at: 1870 1943 2778 3213 3243 3608 1870 Size: 73 835 435 30 365 4545 Fragments arranged by size: 4545 835 435 365 73 30 BamHI G'GATC_C Cuts at: 2029 2164 3369 3613 3980 2029 Size: 135 1205 244 367 4332 Fragments arranged by size: 4332 1205 367 244 135 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2059 2719 3604 5059 618 Size: 325 217 627 272 660 885 1455 1842 Fragments arranged by size: 1842 1455 885 660 627 325 272 217 BanII G_rGCy'C Cuts at: 1391 2504 2703 2988 3412 3462 3602 1391 Size: 1113 199 285 424 50 140 4072 Fragments arranged by size: 4072 1113 424 285 199 140 50 BbeI G_GCGC'C Cuts at: 2063 2063 Size: 6283 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2295 2724 2879 928 Size: 971 396 429 155 4332 Fragments arranged by size: 4332 971 429 396 155 BbvCI CC'TCA_GC Cuts at: 2294 2294 Size: 6283 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2383 2417 4474 4508 1903 Size: 34 446 34 2057 34 3678 Fragments arranged by size: 3678 2057 446 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3347 3416 4175 5385 3347 Size: 69 759 1210 4245 Fragments arranged by size: 4245 1210 759 69 BclI T'GATC_A Cuts at: 1548 1548 Size: 6283 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2912 4894 136 Size: 107 122 71 631 605 1240 1982 1525 Fragments arranged by size: 1982 1525 1240 631 605 122 107 71 BglII A'GATC_T Cuts at: 6278 6278 Size: 6283 BlpI GC'TnA_GC Cuts at: 3700 3700 Size: 6283 Bme1580I G_kGCm'C Cuts at: 1790 2724 4344 5500 1790 Size: 934 1620 1156 2573 Fragments arranged by size: 2573 1620 1156 934 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6283 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4014 4972 442 Size: 416 53 83 399 24 2597 958 1753 Fragments arranged by size: 2597 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6283 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2251 2251 Size: 6283 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2078 2441 2816 3434 4943 1239 Size: 839 363 375 618 1509 2579 Fragments arranged by size: 2579 1509 839 618 375 363 Bpu10I CC'TnA_GC Cuts at: 2294 2294 Size: 6283 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2002 2774 4408 5273 5571 2002 Size: 772 1634 865 298 2714 Fragments arranged by size: 2714 1634 865 772 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2519 3138 3175 4946 882 Size: 617 1020 619 37 1771 2219 Fragments arranged by size: 2219 1771 1020 619 617 37 BsaAI yAC'GTr Cuts at: 493 1204 2158 4074 493 Size: 711 954 1916 2702 Fragments arranged by size: 2702 1916 954 711 BsaBI GATnn'nnATC Cuts at: 3350 6277 3350 Size: 2927 3356 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2060 3321 4090 4472 Size: 53 83 186 936 527 1261 769 382 Cuts at: 4472 5107 275 Size: 635 1451 Fragments arranged by size: 1451 1261 936 769 635 527 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2604 3426 3496 4716 5114 5388 5535 5946 2604 Size: 822 70 1220 398 274 147 411 2941 Fragments arranged by size: 2941 1220 822 411 398 274 147 70 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2571 2601 3284 3314 1178 Size: 30 165 30 1168 30 683 30 4147 Fragments arranged by size: 4147 1168 683 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 2444 3470 3515 1186 Size: 880 378 1026 45 3954 Fragments arranged by size: 3954 1026 880 378 45 BseYI C'CCAG_C Cuts at: 2497 2813 5486 5982 2497 Size: 316 2673 496 2798 Fragments arranged by size: 2798 2673 496 316 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5710 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3367 3509 3733 4494 5522 1540 Size: 275 186 1366 142 224 761 1028 2301 Fragments arranged by size: 2301 1366 1028 761 275 224 186 142 BsiHKAI G_wGCw'C Cuts at: 3412 3462 4344 4429 5500 3412 Size: 50 882 85 1071 4195 Fragments arranged by size: 4195 1071 882 85 50 BsmI GAATG_Cn' Cuts at: 3794 3887 3794 Size: 93 6190 BsmBI CGTCTCn'nnnn_ Cuts at: 2040 2040 Size: 6283 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2504 2703 2724 2988 3412 3462 3602 Size: 399 714 199 21 264 424 50 140 Cuts at: 3602 4344 4429 5500 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 714 424 399 264 199 140 85 50 21 BspEI T'CCGG_A Cuts at: 2604 3496 5946 2604 Size: 892 2450 2941 Fragments arranged by size: 2941 2450 892 BspHI T'CATG_A Cuts at: 2508 3471 4172 5902 2508 Size: 963 701 1730 2889 Fragments arranged by size: 2889 1730 963 701 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3638 844 Size: 2794 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2056 3505 4170 1394 Size: 429 233 1449 665 3507 Fragments arranged by size: 3507 1449 665 429 233 BsrDI GCAATG_nn' Cuts at: 65 1248 3065 4778 4952 65 Size: 1183 1817 1713 174 1396 Fragments arranged by size: 1817 1713 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4927 5114 1700 Size: 110 3117 187 2869 Fragments arranged by size: 3117 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6283 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6283 BssSI C'ACGA_G Cuts at: 4343 5355 4343 Size: 1012 5271 BstAPI GCAn_nnn'nTGC Cuts at: 794 3067 794 Size: 2273 4010 BstBI TT'CG_AA Cuts at: 3516 5087 3516 Size: 1571 4712 BstEII G'GTnAC_C Cuts at: 1184 2974 1184 Size: 1790 4493 BstXI CCAn_nnnn'nTGG Cuts at: 3166 3166 Size: 6283 BstYI r'GATC_y Cuts at: 1972 2029 2164 2601 2772 3369 3613 3980 4365 Size: 57 135 437 171 597 244 367 385 Cuts at: 4365 4382 5834 5920 5932 6278 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 597 437 385 367 346 244 171 135 86 57 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4792 Fragments arranged by size: 4792 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5544 Fragments arranged by size: 5544 683 56 BtsI GCAGTG_nn' Cuts at: 2874 3799 4593 4613 2874 Size: 925 794 20 4544 Fragments arranged by size: 4544 925 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6283 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6248 DraI TTT'AAA Cuts at: 3587 3587 Size: 6283 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2954 5290 809 Size: 507 1638 2336 1802 Fragments arranged by size: 2336 1802 1638 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2364 2838 3012 Size: 54 1425 50 275 18 534 474 174 Cuts at: 3012 3364 3506 3552 3730 4619 6248 8 Size: 352 142 46 178 889 1629 43 Fragments arranged by size: 1629 1425 889 534 474 352 275 178 174 142 54 50 46 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3364 3506 3730 1537 Size: 275 1552 142 224 4090 Fragments arranged by size: 4090 1552 275 224 142 EarI CTCTTCn'nnn_ Cuts at: 1353 4213 1353 Size: 2860 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4870 5244 5390 387 Size: 1064 138 3281 374 146 1280 Fragments arranged by size: 3281 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3410 3460 3410 Size: 50 6233 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2078 2441 2635 2816 3303 3417 3434 Size: 393 446 363 194 181 487 114 17 Cuts at: 3434 4346 4943 5730 5998 1239 Size: 912 597 787 268 1524 Fragments arranged by size: 1524 912 787 597 487 446 393 363 268 194 181 114 17 EcoNI CCTnn'n_nnAGG Cuts at: 2915 3378 2915 Size: 463 5820 EcoO109I rG'GnC_Cy Cuts at: 2189 2189 Size: 6283 EcoRI G'AATT_C Cuts at: 3592 3970 3592 Size: 378 5905 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2562 2562 Size: 6283 FspI TGC'GCA Cuts at: 4789 4789 Size: 6283 HaeII r_GCGC'y Cuts at: 1678 2017 2063 2136 2473 5430 1678 Size: 339 46 73 337 2957 2531 Fragments arranged by size: 2957 2531 339 337 73 46 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2156 2188 2920 2952 3311 Size: 32 135 32 584 32 732 32 359 Cuts at: 3311 3343 3394 3426 4924 4956 4998 5030 1373 Size: 32 51 32 1498 32 42 32 2626 Fragments arranged by size: 2626 1498 732 584 359 135 51 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2413 3627 3873 669 Size: 988 281 475 1214 246 3079 Fragments arranged by size: 3079 1214 988 475 281 246 HindIII A'AGCT_T Cuts at: 748 3022 748 Size: 2274 4009 HpaI GTT'AAC Cuts at: 3873 3873 Size: 6283 KasI G'GCGC_C Cuts at: 2059 2059 Size: 6283 KpnI G_GTAC'C Cuts at: 3608 3608 Size: 6283 MfeI C'AATT_G Cuts at: 3882 3882 Size: 6283 MluI A'CGCG_T Cuts at: 6159 6159 Size: 6283 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2080 5397 5581 1305 Size: 775 3317 184 2007 Fragments arranged by size: 3317 2007 775 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2366 2840 3014 10 Size: 54 1425 343 534 474 174 3279 Fragments arranged by size: 3279 1425 534 474 343 174 54 MslI CAynn'nnrTG Cuts at: 518 1166 2249 2861 2897 3473 4241 4600 4759 Size: 648 1083 612 36 576 768 359 159 Cuts at: 4759 518 Size: 2042 Fragments arranged by size: 2042 1083 768 648 612 576 359 159 36 MspA1I CmG'CkG Cuts at: 1606 3699 4376 5524 5769 1606 Size: 2093 677 1148 245 2120 Fragments arranged by size: 2120 2093 1148 677 245 NarI GG'CG_CC Cuts at: 2060 2060 Size: 6283 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4792 Fragments arranged by size: 4792 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6283 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6283 NotI GC'GGCC_GC Cuts at: 3730 3730 Size: 6283 NspI r_CATG'y Cuts at: 1132 1229 2497 2731 2896 3641 5186 1132 Size: 97 1268 234 165 745 1545 2229 Fragments arranged by size: 2229 1545 1268 745 234 165 97 PasI CC'CwG_GG Cuts at: 1348 3380 1348 Size: 2032 4251 PciI A'CATG_T Cuts at: 5182 5182 Size: 6283 PflMI CCAn_nnn'nTGG Cuts at: 1108 2320 2479 3270 1108 Size: 1212 159 791 4121 Fragments arranged by size: 4121 1212 791 159 PfoI T'CCnGG_A Cuts at: 2514 2514 Size: 6283 PmeI GTTT'AAAC Cuts at: 3587 3587 Size: 6283 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4352 4384 5865 5897 4352 Size: 32 1481 32 4738 Fragments arranged by size: 4738 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6283 PsiI TTA'TAA Cuts at: 3853 3853 Size: 6283 PstI C_TGCA'G Cuts at: 830 2664 2943 3635 830 Size: 1834 279 692 3478 Fragments arranged by size: 3478 1834 692 279 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6283 SacI G_AGCT'C Cuts at: 3412 3462 3412 Size: 50 6233 SalI G'TCGA_C Cuts at: 1936 3625 1936 Size: 1689 4594 SbfI CC_TGCA'GG Cuts at: 3635 3635 Size: 6283 ScaI AGT'ACT Cuts at: 4531 6243 4531 Size: 1712 4571 SfcI C'TryA_G Cuts at: 826 1045 2660 2939 3631 4766 5447 5638 826 Size: 219 1615 279 692 1135 681 191 1471 Fragments arranged by size: 1615 1471 1135 692 681 279 219 191 SfoI GGC'GCC Cuts at: 2061 2061 Size: 6283 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6283 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6283 SmaI CCC'GGG Cuts at: 1872 3215 3610 1872 Size: 1343 395 4545 Fragments arranged by size: 4545 1343 395 SmlI C'TyrA_G Cuts at: 820 1017 1943 2017 2789 4387 5288 5550 820 Size: 197 926 74 772 1598 901 262 1553 Fragments arranged by size: 1598 1553 926 901 772 262 197 74 SnaBI TAC'GTA Cuts at: 493 4074 493 Size: 3581 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6283 SphI G_CATG'C Cuts at: 1132 3641 1132 Size: 2509 3774 SspI AAT'ATT Cuts at: 5 52 4207 5 Size: 47 4155 2081 Fragments arranged by size: 4155 2081 47 StuI AGG'CCT Cuts at: 1517 2906 1517 Size: 1389 4894 StyI C'CwwG_G Cuts at: 513 1065 2004 2750 2859 2979 3722 513 Size: 552 939 746 109 120 743 3074 Fragments arranged by size: 3074 939 746 743 552 120 109 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4310 4327 4480 4665 5110 325 Size: 1255 2730 17 153 185 445 1498 Fragments arranged by size: 2730 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3234 4529 6241 Size: 274 80 33 51 1443 1257 1295 1712 Cuts at: 6241 96 Size: 138 Fragments arranged by size: 1712 1443 1295 1257 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2571 2603 6243 6275 2571 Size: 32 3640 32 2579 Fragments arranged by size: 3640 2579 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6283 XbaI T'CTAG_A Cuts at: 3619 3619 Size: 6283 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6283 XmaI C'CCGG_G Cuts at: 1870 3213 3608 1870 Size: 1343 395 4545 Fragments arranged by size: 4545 1343 395 XmnI GAAnn'nnTTC Cuts at: 4412 4412 Size: 6283 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3322 4091 276 Size: 53 83 186 936 1788 769 2468 Fragments arranged by size: 2468 1788 936 769 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI