Restriction map for
pFN21AE2326
(Linear) (Six-base) MAPSORT of: KIEE2326.seq Check: 3319 from: 1 to: 1578
KIEE2326.orf
With 182 enzymes: SgfI *
February 11, 2011 17:15 ..
AatII G_ACGT'C
Cuts at: 0 1319 1578
Size: 1319 259
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 630 1298 1412 1578
Size: 630 668 114 166
Fragments arranged by size:
668 630 166 114
AhdI GACnn_n'nnGTC
Cuts at: 0 1320 1578
Size: 1320 258
AleI CACnn'nnGTG
Cuts at: 0 856 1578
Size: 856 722
AlwNI CAG_nnn'CTG
Cuts at: 0 232 663 708 1276 1578
Size: 232 431 45 568 302
Fragments arranged by size:
568 431 302 232 45
AvaI C'yCGr_G
Cuts at: 0 773 1208 1238 1578
Size: 773 435 30 340
Fragments arranged by size:
773 435 340 30
BamHI G'GATC_C
Cuts at: 0 24 159 1364 1578
Size: 24 135 1205 214
Fragments arranged by size:
1205 214 135 24
BanI G'GyrC_C
Cuts at: 0 54 714 1578
Size: 54 660 864
Fragments arranged by size:
864 660 54
BanII G_rGCy'C
Cuts at: 0 499 698 983 1407 1457 1578
Size: 499 199 285 424 50 121
Fragments arranged by size:
499 424 285 199 121 50
BbeI G_GCGC'C
Cuts at: 0 58 1578
Size: 58 1520
BbsI GAAGACnn'nnnn_
Cuts at: 0 290 719 874 1578
Size: 290 429 155 704
Fragments arranged by size:
704 429 290 155
BbvCI CC'TCA_GC
Cuts at: 0 289 1578
Size: 289 1289
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 378 412 1578
Size: 378 34 1166
Fragments arranged by size:
1166 378 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1342 1411 1578
Size: 1342 69 167
Fragments arranged by size:
1342 167 69
BglI GCCn_nnn'nGGC
Cuts at: 0 907 1578
Size: 907 671
Bme1580I G_kGCm'C
Cuts at: 0 719 1578
Size: 719 859
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 246 1578
Size: 246 1332
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 436 811 1429 1578
Size: 73 363 375 618 149
Fragments arranged by size:
618 375 363 149 73
Bpu10I CC'TnA_GC
Cuts at: 0 289 1578
Size: 289 1289
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 1578
Size: 769 809
BsaI GGTCTCn'nnnn_
Cuts at: 0 514 1133 1170 1578
Size: 514 619 37 408
Fragments arranged by size:
619 514 408 37
BsaAI yAC'GTr
Cuts at: 0 153 1578
Size: 153 1425
BsaBI GATnn'nnATC
Cuts at: 0 1345 1578
Size: 1345 233
BsaHI Gr'CG_yC
Cuts at: 0 55 1316 1578
Size: 55 1261 262
Fragments arranged by size:
1261 262 55
BsaWI w'CCGG_w
Cuts at: 0 599 1421 1491 1578
Size: 599 822 70 87
Fragments arranged by size:
822 599 87 70
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 566 596 1279 1309 1578
Size: 566 30 683 30 269
Fragments arranged by size:
683 566 269 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 439 1465 1510 1578
Size: 61 378 1026 45 68
Fragments arranged by size:
1026 378 68 61 45
BseYI C'CCAG_C
Cuts at: 0 492 808 1578
Size: 492 316 770
Fragments arranged by size:
770 492 316
BsiEI CG_ry'CG
Cuts at: 0 1362 1504 1578
Size: 1362 142 74
Fragments arranged by size:
1362 142 74
BsiHKAI G_wGCw'C
Cuts at: 0 1407 1457 1578
Size: 1407 50 121
Fragments arranged by size:
1407 121 50
BsmBI CGTCTCn'nnnn_
Cuts at: 0 35 1578
Size: 35 1543
Bsp1286I G_dGCh'C
Cuts at: 0 499 698 719 983 1407 1457 1578
Size: 499 199 21 264 424 50 121
Fragments arranged by size:
499 424 264 199 121 50 21
BspEI T'CCGG_A
Cuts at: 0 599 1491 1578
Size: 599 892 87
Fragments arranged by size:
892 599 87
BspHI T'CATG_A
Cuts at: 0 503 1466 1578
Size: 503 963 112
Fragments arranged by size:
963 503 112
BsrBI CCG'CTC
Cuts at: 0 51 1500 1578
Size: 51 1449 78
Fragments arranged by size:
1449 78 51
BsrDI GCAATG_nn'
Cuts at: 0 1060 1578
Size: 1060 518
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1062 1578
Size: 1062 516
BstBI TT'CG_AA
Cuts at: 0 1511 1578
Size: 1511 67
BstEII G'GTnAC_C
Cuts at: 0 969 1578
Size: 969 609
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1161 1578
Size: 1161 417
BstYI r'GATC_y
Cuts at: 0 24 159 596 767 1364 1578
Size: 24 135 437 171 597 214
Fragments arranged by size:
597 437 214 171 135 24
BtsI GCAGTG_nn'
Cuts at: 0 869 1578
Size: 869 709
DrdI GACnn_nn'nnGTC
Cuts at: 0 949 1578
Size: 949 629
EaeI y'GGCC_r
Cuts at: 0 359 833 1007 1359 1501 1547 1578
Size: 359 474 174 352 142 46 31
Fragments arranged by size:
474 359 352 174 142 46 31
EagI C'GGCC_G
Cuts at: 0 1359 1501 1578
Size: 1359 142 77
Fragments arranged by size:
1359 142 77
EcoICRI GAG'CTC
Cuts at: 0 1405 1455 1578
Size: 1405 50 123
Fragments arranged by size:
1405 123 50
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 436 630 811 1298 1412 1429 1578
Size: 73 363 194 181 487 114 17 149
Fragments arranged by size:
487 363 194 181 149 114 73 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 910 1373 1578
Size: 910 463 205
Fragments arranged by size:
910 463 205
EcoO109I rG'GnC_Cy
Cuts at: 0 184 1578
Size: 184 1394
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 557 1578
Size: 557 1021
HaeII r_GCGC'y
Cuts at: 0 12 58 131 468 1578
Size: 12 46 73 337 1110
Fragments arranged by size:
1110 337 73 46 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 151 183 915 947 1306 1338 1389 1421
Size: 151 32 732 32 359 32 51 32
Cuts at: 1421 1578
Size: 157
Fragments arranged by size:
732 359 157 151 51 32 32 32
32
HincII GTy'rAC
Cuts at: 0 408 1578
Size: 408 1170
HindIII A'AGCT_T
Cuts at: 0 1017 1578
Size: 1017 561
KasI G'GCGC_C
Cuts at: 0 54 1578
Size: 54 1524
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 1578
Size: 75 1503
MscI TGG'CCA
Cuts at: 0 361 835 1009 1578
Size: 361 474 174 569
Fragments arranged by size:
569 474 361 174
MslI CAynn'nnrTG
Cuts at: 0 244 856 892 1468 1578
Size: 244 612 36 576 110
Fragments arranged by size:
612 576 244 110 36
NarI GG'CG_CC
Cuts at: 0 55 1578
Size: 55 1523
NspI r_CATG'y
Cuts at: 0 492 726 891 1578
Size: 492 234 165 687
Fragments arranged by size:
687 492 234 165
PasI CC'CwG_GG
Cuts at: 0 1375 1578
Size: 1375 203
PflMI CCAn_nnn'nTGG
Cuts at: 0 315 474 1265 1578
Size: 315 159 791 313
Fragments arranged by size:
791 315 313 159
PfoI T'CCnGG_A
Cuts at: 0 509 1578
Size: 509 1069
PstI C_TGCA'G
Cuts at: 0 659 938 1578
Size: 659 279 640
Fragments arranged by size:
659 640 279
SacI G_AGCT'C
Cuts at: 0 1407 1457 1578
Size: 1407 50 121
Fragments arranged by size:
1407 121 50
SfcI C'TryA_G
Cuts at: 0 655 934 1578
Size: 655 279 644
Fragments arranged by size:
655 644 279
SfoI GGC'GCC
Cuts at: 0 56 1578
Size: 56 1522
SmaI CCC'GGG
Cuts at: 0 1210 1578
Size: 1210 368
SmlI C'TyrA_G
Cuts at: 0 12 784 1578
Size: 12 772 794
Fragments arranged by size:
794 772 12
StuI AGG'CCT
Cuts at: 0 901 1578
Size: 901 677
StyI C'CwwG_G
Cuts at: 0 745 854 974 1578
Size: 745 109 120 604
Fragments arranged by size:
745 604 120 109
TatI w'GTAC_w
Cuts at: 0 1229 1578
Size: 1229 349
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 566 598 1578
Size: 566 32 980
Fragments arranged by size:
980 566 32
XmaI C'CCGG_G
Cuts at: 0 1208 1578
Size: 1208 370
ZraI GAC'GTC
Cuts at: 0 1317 1578
Size: 1317 261
Enzymes that do cut:
AatII AcuI AhdI AleI AlwNI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BglI Bme1580I
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BsrBI BsrDI BstAPI BstBI BstEII BstXI
BstYI BtsI DrdI EaeI EagI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HincII HindIII KasI MmeI
MscI MslI NarI NspI PasI PflMI PfoI PstI
SacI SfcI SfoI SmaI SmlI StuI StyI TatI
TstI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BclI BfrBI BglII BlpI BmgBI BmrI
BmtI BsgI BsiWI BsmI BspMI BsrFI BsrGI BssHII
BssSI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DraIII EarI EciI EcoRI EcoRV FseI FspI FspAI
HpaI KpnI MfeI MluI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TaqII Tth111I XbaI XcmI XhoI
XmnI