################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## Acc65I AccI AclI AflII AflIII AhdI Alw44I ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII BamHI BbeI BbrPI BbvCI BcgI BfrI BglI BlpI Bpu1102I BsaAI BsaI BsmBI BsmI BspLU11I BssHII Bst1107I BstAPI BstBI BstZ17I CelII CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoT22I FseI FspI HindIII HpaI KasI KpnI KspI MfeI MluI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SbfI ScaI SfcI SfiI SfoI SfuI SmlI SnaBI SpeI Sse8387I SspI SwaI Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccIII 1709 TCCGGA T^CCGGA AgeI 724 ACCGGT A^CCGGT AlwNI 558 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1633 CCTGAGG CC^T[ACGT]AGG Aor51HI 846 AGCGCT AGC^GCT AspHI 85 GAGCTC G[AT]GC[AT]^C AvrII 1944 CCTAGG C^CTAGG BanI 1999 GGCACC G^G[CT][GA]CC BciVI 1530 GGATAC GTATCC(6/5) BlnI 1944 CCTAGG C^CTAGG BsaBI 1707 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 1709 TCCGGA T^CCGGA BseRI 369 CTCCTC GAGGAG(10/8) BsiEI 1185 CGACCG CG[GA][CT]^CG BsiHKAI 85 GAGCTC G[AT]GC[AT]^C BsmAI 172 GTCTC GTCTC(1/5) Bsp1407I 1590 TGTACA T^GTACA BspDI 2065 ATCGAT AT^CGAT BspEI 1709 TCCGGA T^CCGGA BsrGI 1590 TGTACA T^GTACA BssSI 1814 CTCGTG CACGAG(-5/-1) Bsu36I 1633 CCTGAGG CC^T[ACGT]AGG ClaI 2065 ATCGAT AT^CGAT DsaI 944 CCATGG C^C[GA][CT]GG Eco47III 846 AGCGCT AGC^GCT Eco81I 1633 CCTGAGG CC^T[ACGT]AGG EcoNI 957 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1233 GAATTC G^AATTC EcoRV 1978 GATATC GAT^ATC HaeII 846 AGCGCT [GA]GCGC^[CT] HincII 1495 GTCAAC GT[CT]^[GA]AC HindII 1495 GTCAAC GT[CT]^[GA]AC MamI 1707 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1709 TCCGGA T^CCGGA NaeI 478 GCCGGC GCC^GGC NcoI 944 CCATGG C^CATGG NgoMIV 478 GCCGGC G^CCGGC PinAI 724 ACCGGT A^CCGGT PvuII 1141 CAGCTG CAG^CTG SacI 85 GAGCTC GAGCT^C SapI 1845 GCTCTTC GCTCTTC(1/4) SexAI 1499 ACCTGGT A^CC[AT]GGT SgrAI 723 CACCGGTG C[GA]^CCGG[CT]G SphI 1472 GCATGC GCATG^C SspBI 1590 TGTACA T^GTACA TfiI 864 GATTC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AatII 1303 GACGTC GACGT^C 1404 GACGTC AccII 1170 CGCG CG^CG 1401 CGCG AcyI 1303 GACGTC G[GA]^CG[CT]C 1404 GACGTC ApaI 1029 GGGCCC GGGCC^C 1948 GGGCCC Asp700I 36 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 547 GAAGACATTC AvaI 872 CCCGGG C^[CT]CG[GA]G 1964 CCCGGG BalI 620 TGGCCA TGG^CCA 810 TGGCCA BfaI 10 CTAG C^TAG 1945 CTAG BglII 347 AGATCT A^GATCT 1661 AGATCT BsaHI 1303 GACGTC G[GA]^CG[CT]C 1404 GACGTC BsaWI 724 ACCGGT [AT]^CCGG[AT] 1709 TCCGGA BsgI 1138 GTGCAG GTGCAG(16/14) 1837 GTGCAG BsiWI 1037 CGTACG C^GTACG 1667 CGTACG BsoBI 872 CCCGGG C^[CT]CG[GA]G 1964 CCCGGG BspHI 128 TCATGA T^CATGA 900 TCATGA BspMI 1050 GCAGGT ACCTGC(4/8) 1259 GCAGGT BsrBI 711 GAGCGG CCGCTC(-3/-3) 1759 GAGCGG BstEII 439 GGTAACC G^GT[ACGT]ACC 1918 GGTAACC BstPI 439 GGTAACC G^GT[ACGT]ACC 1918 GGTAACC BstUI 1170 CGCG CG^CG 1401 CGCG EcoO65I 439 GGTAACC G^GT[ACGT]ACC 1918 GGTAACC HgaI 604 GACGC GACGC(5/10) 428 GCGTC Hin1I 1303 GACGTC G[GA]^CG[CT]C 1404 GACGTC MaeI 10 CTAG C^TAG 1945 CTAG MluNI 620 TGGCCA TGG^CCA 810 TGGCCA MscI 620 TGGCCA TGG^CCA 810 TGGCCA MslI 486 CATCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1716 CACAGCGGTG MspA1I 1141 CAGCTG C[AC]G^C[GT]G 1718 CAGCGG MvnI 1170 CGCG CG^CG 1401 CGCG NspI 1154 ACATGC [GA]CATG^[CT] 1472 GCATGC RcaI 128 TCATGA T^CATGA 900 TCATGA SmaI 872 CCCGGG CCC^GGG 1964 CCCGGG StuI 38 AGGCCT AGG^CCT 138 AGGCCT XcmI 1616 CCACAATGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1784 CCACCTTGACCCTGG XmaCI 872 CCCGGG C^CCGGG 1964 CCCGGG XmaI 872 CCCGGG C^CCGGG 1964 CCCGGG XmnI 36 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 547 GAAGACATTC ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AvaII BanII BbsI BbvI BclI BcnI BmyI BpmI BpuAI BsaJI BsiYI BslI BsmFI Bsp1286I BsrDI BsrFI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII