(Circular) (Six-base) MAPSORT of: pFN21AE2057.seq Check: 4614 from: 1 to: 6784 pFN21AE2057 6784 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3046 3046 Size: 6784 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3312 3413 4594 278 Size: 53 83 186 936 1776 101 1181 2468 Fragments arranged by size: 2468 1776 1181 936 186 101 83 53 AccI GT'mk_AC Cuts at: 1937 4127 1937 Size: 2190 4594 Acc65I G'GTAC_C Cuts at: 4105 4105 Size: 6784 AclI AA'CG_TT Cuts at: 1563 4911 5284 1563 Size: 3348 373 3063 Fragments arranged by size: 3348 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2252 2782 3485 3716 3950 4847 6231 6499 Size: 620 530 703 231 234 897 1384 268 Cuts at: 6499 1632 Size: 1917 Fragments arranged by size: 1917 1384 897 703 620 530 268 234 231 AfeI AGC'GCT Cuts at: 2853 2853 Size: 6784 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6587 AflIII A'CryG_T Cuts at: 1203 5683 6660 1203 Size: 4480 977 1327 Fragments arranged by size: 4480 1327 977 AgeI A'CCGG_T Cuts at: 2729 5615 2729 Size: 2886 3898 AhdI GACnn_n'nnGTC Cuts at: 5513 5513 Size: 6784 AleI CACnn'nnGTG Cuts at: 1166 3725 1166 Size: 2559 4225 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6784 AlwNI CAG_nnn'CTG Cuts at: 2568 4527 6099 2568 Size: 1959 1572 3253 Fragments arranged by size: 3253 1959 1572 ApaI G_GGCC'C Cuts at: 3038 3957 3038 Size: 919 5865 ApaLI G'TGCA_C Cuts at: 4841 5997 4841 Size: 1156 5628 ApoI r'AATT_y Cuts at: 1493 2596 2989 3238 4093 4319 4471 6580 1493 Size: 1103 393 249 855 226 152 2109 1697 Fragments arranged by size: 2109 1697 1103 855 393 249 226 152 AseI AT'TA_AT Cuts at: 160 1032 5338 160 Size: 872 4306 1606 Fragments arranged by size: 4306 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6784 AvaI C'yCGr_G Cuts at: 1870 1943 2877 3969 4109 1870 Size: 73 934 1092 140 4545 Fragments arranged by size: 4545 1092 934 140 73 AvrII C'CTAG_G Cuts at: 3949 3949 Size: 6784 BamHI G'GATC_C Cuts at: 4114 4481 4114 Size: 367 6417 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4004 4105 5560 618 Size: 325 217 627 2217 101 1455 1842 Fragments arranged by size: 2217 1842 1455 627 325 217 101 BanII G_rGCy'C Cuts at: 1391 2094 3038 3447 3957 4103 1391 Size: 703 944 409 510 146 4072 Fragments arranged by size: 4072 944 703 510 409 146 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2559 3405 3548 928 Size: 971 660 846 143 4164 Fragments arranged by size: 4164 971 846 660 143 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4975 5009 1903 Size: 34 3038 34 3678 Fragments arranged by size: 3678 3038 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3529 4676 5886 3529 Size: 1147 1210 4427 Fragments arranged by size: 4427 1210 1147 BclI T'GATC_A Cuts at: 1548 2130 2673 2883 1548 Size: 582 543 210 5449 Fragments arranged by size: 5449 582 543 210 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5395 136 Size: 107 122 71 631 605 3723 1525 Fragments arranged by size: 3723 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2352 3666 6779 2352 Size: 1314 3113 2357 Fragments arranged by size: 3113 2357 1314 BlpI GC'TnA_GC Cuts at: 4201 4201 Size: 6784 Bme1580I G_kGCm'C Cuts at: 1790 3038 3957 4845 6001 1790 Size: 1248 919 888 1156 2573 Fragments arranged by size: 2573 1248 1156 919 888 BmgBI CAC'GTC Cuts at: 1318 3940 1318 Size: 2622 4162 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2798 4515 5473 Size: 416 53 83 399 24 1381 1717 958 Cuts at: 5473 442 Size: 1753 Fragments arranged by size: 1753 1717 1381 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6784 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2065 2186 2275 2290 2333 3821 5444 1239 Size: 826 121 89 15 43 1488 1623 2579 Fragments arranged by size: 2579 1623 1488 826 121 89 43 15 Bpu10I CC'TnA_GC Cuts at: 3527 3527 Size: 6784 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4909 5774 6072 4909 Size: 865 298 5621 Fragments arranged by size: 5621 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5447 882 Size: 617 3948 2219 Fragments arranged by size: 3948 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4575 493 Size: 711 3371 2702 Fragments arranged by size: 3371 2702 711 BsaBI GATnn'nnATC Cuts at: 3716 6778 3716 Size: 3062 3722 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3309 3410 4591 4973 Size: 53 83 186 936 1776 101 1181 382 Cuts at: 4973 5608 275 Size: 635 1451 Fragments arranged by size: 1776 1451 1181 936 635 382 186 101 83 53 BsaWI w'CCGG_w Cuts at: 2729 3714 5217 5615 5889 6036 6447 2729 Size: 985 1503 398 274 147 411 3066 Fragments arranged by size: 3066 1503 985 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2063 2093 1178 Size: 30 165 30 660 30 5869 Fragments arranged by size: 5869 660 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2365 1186 Size: 1179 5605 BseYI C'CCAG_C Cuts at: 2705 2823 2834 3446 5987 6483 2705 Size: 118 11 612 2541 496 3006 Fragments arranged by size: 3006 2541 612 496 118 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3164 3863 1163 Size: 573 1428 699 4084 Fragments arranged by size: 4084 1428 699 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3193 4234 4995 6023 1540 Size: 275 186 1192 1041 761 1028 2301 Fragments arranged by size: 2301 1192 1041 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2094 4845 4930 6001 2094 Size: 2751 85 1071 2877 Fragments arranged by size: 2877 2751 1071 85 BsiWI C'GTAC_G Cuts at: 3042 3672 3042 Size: 630 6154 BsmI GAATG_Cn' Cuts at: 4295 4388 4295 Size: 93 6691 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2094 3038 3447 3957 4103 4845 4930 Size: 399 304 944 409 510 146 742 85 Cuts at: 4930 6001 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 944 742 510 409 399 304 146 85 BspEI T'CCGG_A Cuts at: 3714 6447 3714 Size: 2733 4051 BspHI T'CATG_A Cuts at: 2133 2905 4673 6403 2133 Size: 772 1768 1730 2514 Fragments arranged by size: 2514 1768 1730 772 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3046 3255 4139 844 Size: 2202 209 884 3489 Fragments arranged by size: 3489 2202 884 209 BsrBI CCG'CTC Cuts at: 1394 1823 2718 3766 4671 1394 Size: 429 895 1048 905 3507 Fragments arranged by size: 3507 1048 905 895 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2284 2689 3301 5279 5453 65 Size: 1183 1036 405 612 1978 174 1396 Fragments arranged by size: 1978 1396 1183 1036 612 405 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2483 2729 3874 5428 5615 1700 Size: 110 673 246 1145 1554 187 2869 Fragments arranged by size: 2869 1554 1145 673 246 187 110 BsrGI T'GTAC_A Cuts at: 96 3595 96 Size: 3499 3285 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6784 BssSI C'ACGA_G Cuts at: 3819 4844 5856 3819 Size: 1025 1012 4747 Fragments arranged by size: 4747 1025 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6784 BstBI TT'CG_AA Cuts at: 5588 5588 Size: 6784 BstEII G'GTnAC_C Cuts at: 1184 2444 3923 1184 Size: 1260 1479 4045 Fragments arranged by size: 4045 1479 1260 BstXI CCAn_nnnn'nTGG Cuts at: 2751 3286 3865 2751 Size: 535 579 5670 Fragments arranged by size: 5670 579 535 BstYI r'GATC_y Cuts at: 1972 2352 2769 3666 4114 4481 4866 4883 6335 Size: 380 417 897 448 367 385 17 1452 Cuts at: 6335 6421 6433 6779 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 897 448 417 385 380 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3639 3639 Size: 6784 BtgI C'CryG_G Cuts at: 513 1065 2004 2949 513 Size: 552 939 945 4348 Fragments arranged by size: 4348 945 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6045 Fragments arranged by size: 6045 683 56 BtsI GCAGTG_nn' Cuts at: 4300 5094 5114 4300 Size: 794 20 5970 Fragments arranged by size: 5970 794 20 ClaI AT'CG_AT Cuts at: 1554 4071 1554 Size: 2517 4267 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6749 DraI TTT'AAA Cuts at: 4088 4088 Size: 6784 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5791 809 Size: 507 4475 1802 Fragments arranged by size: 4475 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2205 2625 2653 Size: 54 1425 50 275 18 375 420 28 Cuts at: 2653 2734 2815 4231 5120 6749 8 Size: 81 81 1416 889 1629 43 Fragments arranged by size: 1629 1425 1416 889 420 375 275 81 81 54 50 43 28 18 EagI C'GGCC_G Cuts at: 1537 1812 4231 1537 Size: 275 2419 4090 Fragments arranged by size: 4090 2419 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2457 3639 3857 4714 1353 Size: 1104 1182 218 857 3423 Fragments arranged by size: 3423 1182 1104 857 218 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2591 5371 5745 5891 387 Size: 1064 138 1002 2780 374 146 1280 Fragments arranged by size: 2780 1280 1064 1002 374 146 138 EcoICRI GAG'CTC Cuts at: 2092 2092 Size: 6784 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2065 2186 2252 2275 2290 2333 2782 Size: 393 433 121 66 23 15 43 449 Cuts at: 2782 3485 3716 3821 3950 4847 5444 6231 6499 Size: 703 231 105 129 897 597 787 268 Cuts at: 6499 1239 Size: 1524 Fragments arranged by size: 1524 897 787 703 597 449 433 393 268 231 129 121 105 66 43 23 15 EcoNI CCTnn'n_nnAGG Cuts at: 2966 2966 Size: 6784 EcoO109I rG'GnC_Cy Cuts at: 2186 2740 2838 3035 3736 3953 3966 2186 Size: 554 98 197 701 217 13 5004 Fragments arranged by size: 5004 701 554 217 197 98 13 EcoRI G'AATT_C Cuts at: 3238 4093 4471 3238 Size: 855 378 5551 Fragments arranged by size: 5551 855 378 EcoRV GAT'ATC Cuts at: 3985 3985 Size: 6784 FspI TGC'GCA Cuts at: 5290 5290 Size: 6784 HaeII r_GCGC'y Cuts at: 1678 2855 5931 1678 Size: 1177 3076 2531 Fragments arranged by size: 3076 2531 1177 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2063 2095 5425 5457 5499 Size: 32 135 32 491 32 3330 32 42 Cuts at: 5499 5531 1373 Size: 32 2626 Fragments arranged by size: 3330 2626 491 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3502 4128 4374 669 Size: 988 281 1564 626 246 3079 Fragments arranged by size: 3079 1564 988 626 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6784 HpaI GTT'AAC Cuts at: 4374 4374 Size: 6784 KpnI G_GTAC'C Cuts at: 4109 4109 Size: 6784 MfeI C'AATT_G Cuts at: 4383 4383 Size: 6784 MluI A'CGCG_T Cuts at: 6660 6660 Size: 6784 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3042 5898 6082 1305 Size: 1737 2856 184 2007 Fragments arranged by size: 2856 2007 1737 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2627 2817 10 Size: 54 1425 343 795 190 3977 Fragments arranged by size: 3977 1425 795 343 190 54 MslI CAynn'nnrTG Cuts at: 518 1166 2495 3725 4742 5101 5260 518 Size: 648 1329 1230 1017 359 159 2042 Fragments arranged by size: 2042 1329 1230 1017 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3148 3725 4200 4877 6025 6270 1606 Size: 1542 577 475 677 1148 245 2120 Fragments arranged by size: 2120 1542 1148 677 577 475 245 NaeI GCC'GGC Cuts at: 2485 2485 Size: 6784 NcoI C'CATG_G Cuts at: 513 1065 2004 2949 513 Size: 552 939 945 4348 Fragments arranged by size: 4348 945 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6784 NgoMIV G'CCGG_C Cuts at: 2483 2483 Size: 6784 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6784 NotI GC'GGCC_GC Cuts at: 4231 4231 Size: 6784 NspI r_CATG'y Cuts at: 1132 1229 3163 3481 4142 5687 1132 Size: 97 1934 318 661 1545 2229 Fragments arranged by size: 2229 1934 1545 661 318 97 PasI CC'CwG_GG Cuts at: 1348 2362 1348 Size: 1014 5770 PciI A'CATG_T Cuts at: 5683 5683 Size: 6784 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6784 PfoI T'CCnGG_A Cuts at: 3777 3995 3777 Size: 218 6566 PmeI GTTT'AAAC Cuts at: 4088 4088 Size: 6784 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4853 4885 6366 6398 4853 Size: 32 1481 32 5239 Fragments arranged by size: 5239 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2740 2838 3736 3966 2740 Size: 98 898 230 5558 Fragments arranged by size: 5558 898 230 98 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6784 PsiI TTA'TAA Cuts at: 4354 4354 Size: 6784 PspOMI G'GGCC_C Cuts at: 3034 3953 3034 Size: 919 5865 PstI C_TGCA'G Cuts at: 830 4136 830 Size: 3306 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6784 PvuII CAG'CTG Cuts at: 3148 3148 Size: 6784 SacI G_AGCT'C Cuts at: 2094 2094 Size: 6784 SalI G'TCGA_C Cuts at: 1936 4126 1936 Size: 2190 4594 SanDI GG'GwC_CC Cuts at: 2740 2838 3966 2740 Size: 98 1128 5558 Fragments arranged by size: 5558 1128 98 SapI GCTCTTCn'nnn_ Cuts at: 3857 3857 Size: 6784 SbfI CC_TGCA'GG Cuts at: 4136 4136 Size: 6784 ScaI AGT'ACT Cuts at: 5032 6744 5032 Size: 1712 5072 SexAI A'CCwGG_T Cuts at: 3504 3504 Size: 6784 SfcI C'TryA_G Cuts at: 826 1045 4132 5267 5948 6139 826 Size: 219 3087 1135 681 191 1471 Fragments arranged by size: 3087 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6784 SgrAI Cr'CCGG_yG Cuts at: 1700 2729 1700 Size: 1029 5755 SmaI CCC'GGG Cuts at: 1872 2879 3971 4111 1872 Size: 1007 1092 140 4545 Fragments arranged by size: 4545 1092 1007 140 SmlI C'TyrA_G Cuts at: 820 1017 1943 4888 5789 6051 820 Size: 197 926 2945 901 262 1553 Fragments arranged by size: 2945 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4575 493 Size: 4082 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6784 SphI G_CATG'C Cuts at: 1132 3481 4142 1132 Size: 2349 661 3774 Fragments arranged by size: 3774 2349 661 SspI AAT'ATT Cuts at: 5 52 4708 5 Size: 47 4656 2081 Fragments arranged by size: 4656 2081 47 StuI AGG'CCT Cuts at: 1517 2045 2145 1517 Size: 528 100 6156 Fragments arranged by size: 6156 528 100 StyI C'CwwG_G Cuts at: 513 1065 2004 2146 2744 2949 3088 3279 3949 Size: 552 939 142 598 205 139 191 670 Cuts at: 3949 3957 4223 513 Size: 8 266 3074 Fragments arranged by size: 3074 939 670 598 552 266 205 191 142 139 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2338 2931 3106 4811 4828 4981 5166 Size: 1255 758 593 175 1705 17 153 185 Cuts at: 5166 5611 325 Size: 445 1498 Fragments arranged by size: 1705 1498 1255 758 593 445 185 175 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2937 3321 3595 Size: 274 80 33 51 1443 960 384 274 Cuts at: 3595 5030 6742 96 Size: 1435 1712 138 Fragments arranged by size: 1712 1443 1435 960 384 274 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3042 3074 6744 6776 3042 Size: 32 3670 32 3050 Fragments arranged by size: 3670 3050 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6784 XbaI T'CTAG_A Cuts at: 4120 4120 Size: 6784 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3628 3796 3628 Size: 168 6616 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6784 XmaI C'CCGG_G Cuts at: 1870 2877 3969 4109 1870 Size: 1007 1092 140 4545 Fragments arranged by size: 4545 1092 1007 140 XmnI GAAnn'nnTTC Cuts at: 2045 2556 4913 2045 Size: 511 2357 3916 Fragments arranged by size: 3916 2357 511 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3310 3411 4592 276 Size: 53 83 186 936 1776 101 1181 2468 Fragments arranged by size: 2468 1776 1181 936 186 101 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbeI BbvCI BfrBI BplI BsmBI BstZ17I DraIII FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SfiI SfoI SrfI SwaI