(Linear) (Six-base) MAPSORT of: KIEE2057.seq Check: 1105 from: 1 to: 2079 KIEE2057.orf With 182 enzymes: SgfI * July 15, 2010 18:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1041 2079 Size: 1041 1038 AatII G_ACGT'C Cuts at: 0 1307 1408 2079 Size: 1307 101 671 Fragments arranged by size: 1307 671 101 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 247 777 1480 1711 1945 2079 Size: 247 530 703 231 234 134 Fragments arranged by size: 703 530 247 234 231 134 AfeI AGC'GCT Cuts at: 0 848 2079 Size: 848 1231 AgeI A'CCGG_T Cuts at: 0 724 2079 Size: 724 1355 AleI CACnn'nnGTG Cuts at: 0 1720 2079 Size: 1720 359 AlwNI CAG_nnn'CTG Cuts at: 0 563 2079 Size: 563 1516 ApaI G_GGCC'C Cuts at: 0 1033 1952 2079 Size: 1033 919 127 Fragments arranged by size: 1033 919 127 ApoI r'AATT_y Cuts at: 0 591 984 1233 2079 Size: 591 393 249 846 Fragments arranged by size: 846 591 393 249 AvaI C'yCGr_G Cuts at: 0 872 1964 2079 Size: 872 1092 115 Fragments arranged by size: 1092 872 115 AvrII C'CTAG_G Cuts at: 0 1944 2079 Size: 1944 135 BanI G'GyrC_C Cuts at: 0 1999 2079 Size: 1999 80 BanII G_rGCy'C Cuts at: 0 89 1033 1442 1952 2079 Size: 89 944 409 510 127 Fragments arranged by size: 944 510 409 127 89 BbsI GAAGACnn'nnnn_ Cuts at: 0 554 1400 1543 2079 Size: 554 846 143 536 Fragments arranged by size: 846 554 536 143 BciVI GTATCCnnnnn_n' Cuts at: 0 1524 2079 Size: 1524 555 BclI T'GATC_A Cuts at: 0 125 668 878 2079 Size: 125 543 210 1201 Fragments arranged by size: 1201 543 210 125 BglII A'GATC_T Cuts at: 0 347 1661 2079 Size: 347 1314 418 Fragments arranged by size: 1314 418 347 Bme1580I G_kGCm'C Cuts at: 0 1033 1952 2079 Size: 1033 919 127 Fragments arranged by size: 1033 919 127 BmgBI CAC'GTC Cuts at: 0 1935 2079 Size: 1935 144 BmrI ACTGGGnnnn_n' Cuts at: 0 793 2079 Size: 793 1286 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 60 181 270 285 328 1816 2079 Size: 60 121 89 15 43 1488 263 Fragments arranged by size: 1488 263 121 89 60 43 15 Bpu10I CC'TnA_GC Cuts at: 0 1522 2079 Size: 1522 557 BsaBI GATnn'nnATC Cuts at: 0 1711 2079 Size: 1711 368 BsaHI Gr'CG_yC Cuts at: 0 1304 1405 2079 Size: 1304 101 674 Fragments arranged by size: 1304 674 101 BsaWI w'CCGG_w Cuts at: 0 724 1709 2079 Size: 724 985 370 Fragments arranged by size: 985 724 370 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 58 88 2079 Size: 58 30 1991 Fragments arranged by size: 1991 58 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 360 2079 Size: 360 1719 BseYI C'CCAG_C Cuts at: 0 700 818 829 1441 2079 Size: 700 118 11 612 638 Fragments arranged by size: 700 638 612 118 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1159 1858 2079 Size: 1159 699 221 Fragments arranged by size: 1159 699 221 BsiEI CG_ry'CG Cuts at: 0 1188 2079 Size: 1188 891 BsiHKAI G_wGCw'C Cuts at: 0 89 2079 Size: 89 1990 BsiWI C'GTAC_G Cuts at: 0 1037 1667 2079 Size: 1037 630 412 Fragments arranged by size: 1037 630 412 Bsp1286I G_dGCh'C Cuts at: 0 89 1033 1442 1952 2079 Size: 89 944 409 510 127 Fragments arranged by size: 944 510 409 127 89 BspEI T'CCGG_A Cuts at: 0 1709 2079 Size: 1709 370 BspHI T'CATG_A Cuts at: 0 128 900 2079 Size: 128 772 1179 Fragments arranged by size: 1179 772 128 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1041 1250 2079 Size: 1041 209 829 Fragments arranged by size: 1041 829 209 BsrBI CCG'CTC Cuts at: 0 713 1761 2079 Size: 713 1048 318 Fragments arranged by size: 1048 713 318 BsrDI GCAATG_nn' Cuts at: 0 279 684 1296 2079 Size: 279 405 612 783 Fragments arranged by size: 783 612 405 279 BsrFI r'CCGG_y Cuts at: 0 478 724 1869 2079 Size: 478 246 1145 210 Fragments arranged by size: 1145 478 246 210 BsrGI T'GTAC_A Cuts at: 0 1590 2079 Size: 1590 489 BssSI C'ACGA_G Cuts at: 0 1814 2079 Size: 1814 265 BstEII G'GTnAC_C Cuts at: 0 439 1918 2079 Size: 439 1479 161 Fragments arranged by size: 1479 439 161 BstXI CCAn_nnnn'nTGG Cuts at: 0 746 1281 1860 2079 Size: 746 535 579 219 Fragments arranged by size: 746 579 535 219 BstYI r'GATC_y Cuts at: 0 347 764 1661 2079 Size: 347 417 897 418 Fragments arranged by size: 897 418 417 347 Bsu36I CC'TnA_GG Cuts at: 0 1634 2079 Size: 1634 445 BtgI C'CryG_G Cuts at: 0 944 2079 Size: 944 1135 ClaI AT'CG_AT Cuts at: 0 2066 2079 Size: 2066 13 EaeI y'GGCC_r Cuts at: 0 200 620 648 729 810 2079 Size: 200 420 28 81 81 1269 Fragments arranged by size: 1269 420 200 81 81 28 EarI CTCTTCn'nnn_ Cuts at: 0 452 1634 1852 2079 Size: 452 1182 218 227 Fragments arranged by size: 1182 452 227 218 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 586 2079 Size: 586 1493 EcoICRI GAG'CTC Cuts at: 0 87 2079 Size: 87 1992 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 60 181 247 270 285 328 777 1480 Size: 60 121 66 23 15 43 449 703 Cuts at: 1480 1711 1816 1945 2079 Size: 231 105 129 134 Fragments arranged by size: 703 449 231 134 129 121 105 66 60 43 23 15 EcoNI CCTnn'n_nnAGG Cuts at: 0 961 2079 Size: 961 1118 EcoO109I rG'GnC_Cy Cuts at: 0 181 735 833 1030 1731 1948 1961 2079 Size: 181 554 98 197 701 217 13 118 Fragments arranged by size: 701 554 217 197 181 118 98 13 EcoRI G'AATT_C Cuts at: 0 1233 2079 Size: 1233 846 EcoRV GAT'ATC Cuts at: 0 1980 2079 Size: 1980 99 HaeII r_GCGC'y Cuts at: 0 850 2079 Size: 850 1229 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 58 90 2079 Size: 58 32 1989 Fragments arranged by size: 1989 58 32 HincII GTy'rAC Cuts at: 0 1497 2079 Size: 1497 582 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1037 2079 Size: 1037 1042 MscI TGG'CCA Cuts at: 0 622 812 2079 Size: 622 190 1267 Fragments arranged by size: 1267 622 190 MslI CAynn'nnrTG Cuts at: 0 490 1720 2079 Size: 490 1230 359 Fragments arranged by size: 1230 490 359 MspA1I CmG'CkG Cuts at: 0 1143 1720 2079 Size: 1143 577 359 Fragments arranged by size: 1143 577 359 NaeI GCC'GGC Cuts at: 0 480 2079 Size: 480 1599 NcoI C'CATG_G Cuts at: 0 944 2079 Size: 944 1135 NgoMIV G'CCGG_C Cuts at: 0 478 2079 Size: 478 1601 NspI r_CATG'y Cuts at: 0 1158 1476 2079 Size: 1158 318 603 Fragments arranged by size: 1158 603 318 PasI CC'CwG_GG Cuts at: 0 357 2079 Size: 357 1722 PfoI T'CCnGG_A Cuts at: 0 1772 1990 2079 Size: 1772 218 89 Fragments arranged by size: 1772 218 89 PpuMI rG'GwC_Cy Cuts at: 0 735 833 1731 1961 2079 Size: 735 98 898 230 118 Fragments arranged by size: 898 735 230 118 98 PspOMI G'GGCC_C Cuts at: 0 1029 1948 2079 Size: 1029 919 131 Fragments arranged by size: 1029 919 131 PvuII CAG'CTG Cuts at: 0 1143 2079 Size: 1143 936 SacI G_AGCT'C Cuts at: 0 89 2079 Size: 89 1990 SanDI GG'GwC_CC Cuts at: 0 735 833 1961 2079 Size: 735 98 1128 118 Fragments arranged by size: 1128 735 118 98 SapI GCTCTTCn'nnn_ Cuts at: 0 1852 2079 Size: 1852 227 SexAI A'CCwGG_T Cuts at: 0 1499 2079 Size: 1499 580 SgrAI Cr'CCGG_yG Cuts at: 0 724 2079 Size: 724 1355 SmaI CCC'GGG Cuts at: 0 874 1966 2079 Size: 874 1092 113 Fragments arranged by size: 1092 874 113 SphI G_CATG'C Cuts at: 0 1476 2079 Size: 1476 603 StuI AGG'CCT Cuts at: 0 40 140 2079 Size: 40 100 1939 Fragments arranged by size: 1939 100 40 StyI C'CwwG_G Cuts at: 0 141 739 944 1083 1274 1944 1952 2079 Size: 141 598 205 139 191 670 8 127 Fragments arranged by size: 670 598 205 191 141 139 127 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 333 926 1101 2079 Size: 333 593 175 978 Fragments arranged by size: 978 593 333 175 TatI w'GTAC_w Cuts at: 0 932 1316 1590 2079 Size: 932 384 274 489 Fragments arranged by size: 932 489 384 274 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1037 1069 2079 Size: 1037 32 1010 Fragments arranged by size: 1037 1010 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1623 1791 2079 Size: 1623 168 288 Fragments arranged by size: 1623 288 168 XmaI C'CCGG_G Cuts at: 0 872 1964 2079 Size: 872 1092 115 Fragments arranged by size: 1092 872 115 XmnI GAAnn'nnTTC Cuts at: 0 40 551 2079 Size: 40 511 1528 Fragments arranged by size: 1528 511 40 ZraI GAC'GTC Cuts at: 0 1305 1406 2079 Size: 1305 101 673 Fragments arranged by size: 1305 673 101 Enzymes that do cut: AarI AatII AcuI AfeI AgeI AleI AlwNI ApaI ApoI AvaI AvrII BanI BanII BbsI BciVI BclI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstEII BstXI BstYI Bsu36I BtgI ClaI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I HincII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PfoI PpuMI PspOMI PvuII SacI SanDI SapI SexAI SgrAI SmaI SphI StuI StyI TaqII TatI TstI XcmI XmaI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AflII AflIII AhdI AlfI AloI ApaLI AscI AseI AsiSI BaeI BamHI BbeI BbvCI BcgI BfrBI BglI BlpI BmtI BplI BpuEI BsaI BsaAI BsmI BsmBI BssHII BstAPI BstBI BstZ17I BtgZI BtsI CspCI DraI DraIII DrdI EagI FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI NsiI PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SmlI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI