(Circular) (Six-base) MAPSORT of: pFN21AE1780.seq Check: 5996 from: 1 to: 5569 pFN21AE1780 5569 bp With 182 enzymes: SgfI * June 10, 2016 17:08 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2171 2171 Size: 5569 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3379 278 Size: 53 83 186 936 1843 2468 Fragments arranged by size: 2468 1843 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2912 1937 Size: 975 4594 Acc65I G'GTAC_C Cuts at: 2205 2890 2205 Size: 685 4884 AclI AA'CG_TT Cuts at: 1563 3696 4069 1563 Size: 2133 373 3063 Fragments arranged by size: 3063 2133 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2651 3632 5016 5284 1632 Size: 1019 981 1384 268 1917 Fragments arranged by size: 1917 1384 1019 981 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5372 AflIII A'CryG_T Cuts at: 1203 4468 5445 1203 Size: 3265 977 1327 Fragments arranged by size: 3265 1327 977 AgeI A'CCGG_T Cuts at: 4400 4400 Size: 5569 AhdI GACnn_n'nnGTC Cuts at: 4298 4298 Size: 5569 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5569 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2564 1184 Size: 1380 4189 AlwNI CAG_nnn'CTG Cuts at: 2097 2629 2833 3312 4884 2097 Size: 532 204 479 1572 2782 Fragments arranged by size: 2782 1572 532 479 204 ApaI G_GGCC'C Cuts at: 2344 2344 Size: 5569 ApaLI G'TGCA_C Cuts at: 3626 4782 3626 Size: 1156 4413 ApoI r'AATT_y Cuts at: 1493 2878 3104 3256 5365 1493 Size: 1385 226 152 2109 1697 Fragments arranged by size: 2109 1697 1385 226 152 AseI AT'TA_AT Cuts at: 160 1032 4123 160 Size: 872 3091 1606 Fragments arranged by size: 3091 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5569 AvaI C'yCGr_G Cuts at: 1870 1943 2252 2583 2894 1870 Size: 73 309 331 311 4545 Fragments arranged by size: 4545 331 311 309 73 BamHI G'GATC_C Cuts at: 2899 3266 2899 Size: 367 5202 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2020 2205 2360 2777 2890 Size: 325 217 627 233 185 155 417 113 Cuts at: 2890 4345 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 417 325 233 217 185 155 113 BanII G_rGCy'C Cuts at: 1391 2219 2344 2430 2715 2804 2888 1391 Size: 828 125 86 285 89 84 4072 Fragments arranged by size: 4072 828 285 125 89 86 84 BbeI G_GCGC'C Cuts at: 2024 2024 Size: 5569 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2708 928 Size: 971 809 3789 Fragments arranged by size: 3789 971 809 BbvCI CC'TCA_GC Cuts at: 2644 2644 Size: 5569 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3760 3794 1903 Size: 34 50 34 1739 34 3678 Fragments arranged by size: 3678 1739 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3461 4671 3461 Size: 1210 4359 BclI T'GATC_A Cuts at: 1548 1548 Size: 5569 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2271 2311 2396 Size: 107 122 71 631 605 599 40 85 Cuts at: 2396 2538 2836 4180 136 Size: 142 298 1344 1525 Fragments arranged by size: 1525 1344 631 605 599 298 142 122 107 85 71 40 BglII A'GATC_T Cuts at: 5564 5564 Size: 5569 BlpI GC'TnA_GC Cuts at: 2803 2986 2803 Size: 183 5386 Bme1580I G_kGCm'C Cuts at: 1790 2344 2460 2724 2780 2827 3630 4786 1790 Size: 554 116 264 56 47 803 1156 2573 Fragments arranged by size: 2573 1156 803 554 264 116 56 47 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5569 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2063 3300 4258 Size: 416 53 83 399 24 646 1237 958 Cuts at: 4258 442 Size: 1753 Fragments arranged by size: 1753 1237 958 646 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5569 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2659 2659 Size: 5569 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4229 1239 Size: 2990 2579 Bpu10I CC'TnA_GC Cuts at: 2429 2644 2733 2429 Size: 215 89 5265 Fragments arranged by size: 5265 215 89 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3694 4559 4857 3694 Size: 865 298 4406 Fragments arranged by size: 4406 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4232 882 Size: 617 2733 2219 Fragments arranged by size: 2733 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3360 493 Size: 711 2156 2702 Fragments arranged by size: 2702 2156 711 BsaBI GATnn'nnATC Cuts at: 5563 5563 Size: 5569 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2021 3376 3758 4393 Size: 53 83 186 936 488 1355 382 635 Cuts at: 4393 275 Size: 1451 Fragments arranged by size: 1451 1355 936 635 488 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4002 4400 4674 4821 5232 4002 Size: 398 274 147 411 4339 Fragments arranged by size: 4339 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5344 Fragments arranged by size: 5344 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5569 BseYI C'CCAG_C Cuts at: 2119 2219 2471 2536 2826 4772 5268 2119 Size: 100 252 65 290 1946 496 2420 Fragments arranged by size: 2420 1946 496 290 252 100 65 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4996 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3019 3780 4808 1540 Size: 275 186 1018 761 1028 2301 Fragments arranged by size: 2301 1028 1018 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2430 3630 3715 4786 2430 Size: 1200 85 1071 3213 Fragments arranged by size: 3213 1200 1071 85 BsmI GAATG_Cn' Cuts at: 3080 3173 3080 Size: 93 5476 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2219 2344 2430 2460 2715 2724 2780 Size: 399 429 125 86 30 255 9 56 Cuts at: 2780 2804 2827 2888 3630 3715 4786 1391 Size: 24 23 61 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 429 399 255 125 86 85 61 56 30 24 23 9 BspEI T'CCGG_A Cuts at: 5232 5232 Size: 5569 BspHI T'CATG_A Cuts at: 3458 5188 3458 Size: 1730 3839 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2171 2924 844 Size: 1327 753 3489 Fragments arranged by size: 3489 1327 753 BsrBI CCG'CTC Cuts at: 1394 1823 3456 1394 Size: 429 1633 3507 Fragments arranged by size: 3507 1633 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2356 4064 4238 65 Size: 1183 1108 1708 174 1396 Fragments arranged by size: 1708 1396 1183 1108 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2492 2618 4213 4400 1700 Size: 110 682 126 1595 187 2869 Fragments arranged by size: 2869 1595 682 187 126 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5569 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5569 BssSI C'ACGA_G Cuts at: 2129 2592 3629 4641 2129 Size: 463 1037 1012 3057 Fragments arranged by size: 3057 1037 1012 463 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5569 BstBI TT'CG_AA Cuts at: 4373 4373 Size: 5569 BstEII G'GTnAC_C Cuts at: 1184 2134 1184 Size: 950 4619 BstXI CCAn_nnnn'nTGG Cuts at: 2037 2386 2037 Size: 349 5220 BstYI r'GATC_y Cuts at: 1972 2010 2899 3266 3651 3668 5120 5206 5218 Size: 38 889 367 385 17 1452 86 12 Cuts at: 5218 5564 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 889 385 367 346 86 38 17 12 Bsu36I CC'TnA_GG Cuts at: 2841 2841 Size: 5569 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5017 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4830 Fragments arranged by size: 4830 683 56 BtsI GCAGTG_nn' Cuts at: 2851 3085 3879 3899 2851 Size: 234 794 20 4521 Fragments arranged by size: 4521 794 234 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5569 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5534 DraI TTT'AAA Cuts at: 2873 2873 Size: 5569 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4576 809 Size: 507 3260 1802 Fragments arranged by size: 3260 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2027 2063 2241 Size: 54 1425 50 275 18 197 36 178 Cuts at: 2241 2272 2494 2568 2620 3016 3905 5534 8 Size: 31 222 74 52 396 889 1629 43 Fragments arranged by size: 1629 1425 889 396 275 222 197 178 74 54 52 50 43 36 31 18 EagI C'GGCC_G Cuts at: 1537 1812 3016 1537 Size: 275 1204 4090 Fragments arranged by size: 4090 1204 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3499 1353 Size: 2146 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4156 4530 4676 387 Size: 1064 138 2567 374 146 1280 Fragments arranged by size: 2567 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2428 2428 Size: 5569 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2651 3632 4229 5016 5284 1239 Size: 393 1019 981 597 787 268 1524 Fragments arranged by size: 1524 1019 981 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2482 2633 2482 Size: 151 5418 EcoO109I rG'GnC_Cy Cuts at: 2094 2340 2563 2638 2094 Size: 246 223 75 5025 Fragments arranged by size: 5025 246 223 75 EcoRI G'AATT_C Cuts at: 2878 3256 2878 Size: 378 5191 FspI TGC'GCA Cuts at: 4075 4075 Size: 5569 HaeII r_GCGC'y Cuts at: 1678 2024 4716 1678 Size: 346 2692 2531 Fragments arranged by size: 2692 2531 346 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4210 4242 4284 4316 1373 Size: 32 135 32 2638 32 42 32 2626 Fragments arranged by size: 2638 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2913 3159 669 Size: 988 281 975 246 3079 Fragments arranged by size: 3079 988 975 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5569 HpaI GTT'AAC Cuts at: 3159 3159 Size: 5569 KasI G'GCGC_C Cuts at: 2020 2020 Size: 5569 KpnI G_GTAC'C Cuts at: 2209 2894 2209 Size: 685 4884 MfeI C'AATT_G Cuts at: 3168 3168 Size: 5569 MluI A'CGCG_T Cuts at: 5445 5445 Size: 5569 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2199 4683 4867 1305 Size: 894 2484 184 2007 Fragments arranged by size: 2484 2007 894 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2029 10 Size: 54 1425 343 197 3550 Fragments arranged by size: 3550 1425 343 197 54 MslI CAynn'nnrTG Cuts at: 518 1166 2330 3527 3886 4045 518 Size: 648 1164 1197 359 159 2042 Fragments arranged by size: 2042 1197 1164 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2358 2807 2985 3662 4810 5055 1606 Size: 752 449 178 677 1148 245 2120 Fragments arranged by size: 2120 1148 752 677 449 245 178 NaeI GCC'GGC Cuts at: 2494 2494 Size: 5569 NarI GG'CG_CC Cuts at: 2021 2021 Size: 5569 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5017 NdeI CA'TA_TG Cuts at: 387 387 Size: 5569 NgoMIV G'CCGG_C Cuts at: 2492 2492 Size: 5569 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5569 NotI GC'GGCC_GC Cuts at: 3016 3016 Size: 5569 NspI r_CATG'y Cuts at: 1132 1229 2927 4472 1132 Size: 97 1698 1545 2229 Fragments arranged by size: 2229 1698 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5569 PciI A'CATG_T Cuts at: 4468 4468 Size: 5569 PflMI CCAn_nnn'nTGG Cuts at: 1108 2509 1108 Size: 1401 4168 PmeI GTTT'AAAC Cuts at: 2873 2873 Size: 5569 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3638 3670 5151 5183 3638 Size: 32 1481 32 4024 Fragments arranged by size: 4024 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2563 2638 2563 Size: 75 5494 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5569 PsiI TTA'TAA Cuts at: 3139 3139 Size: 5569 PspOMI G'GGCC_C Cuts at: 2340 2340 Size: 5569 PstI C_TGCA'G Cuts at: 830 2168 2921 830 Size: 1338 753 3478 Fragments arranged by size: 3478 1338 753 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5569 PvuII CAG'CTG Cuts at: 2807 2807 Size: 5569 SacI G_AGCT'C Cuts at: 2430 2430 Size: 5569 SalI G'TCGA_C Cuts at: 1936 2911 1936 Size: 975 4594 SbfI CC_TGCA'GG Cuts at: 2921 2921 Size: 5569 ScaI AGT'ACT Cuts at: 3817 5529 3817 Size: 1712 3857 SfcI C'TryA_G Cuts at: 826 1045 2164 2917 4052 4733 4924 826 Size: 219 1119 753 1135 681 191 1471 Fragments arranged by size: 1471 1135 1119 753 681 219 191 SfoI GGC'GCC Cuts at: 2022 2022 Size: 5569 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5569 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5569 SmaI CCC'GGG Cuts at: 1872 2585 2896 1872 Size: 713 311 4545 Fragments arranged by size: 4545 713 311 SmlI C'TyrA_G Cuts at: 820 1017 1943 3673 4574 4836 820 Size: 197 926 1730 901 262 1553 Fragments arranged by size: 1730 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3360 493 Size: 2867 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5569 SphI G_CATG'C Cuts at: 1132 2927 1132 Size: 1795 3774 SspI AAT'ATT Cuts at: 5 52 3493 5 Size: 47 3441 2081 Fragments arranged by size: 3441 2081 47 StuI AGG'CCT Cuts at: 1517 2839 1517 Size: 1322 4247 StyI C'CwwG_G Cuts at: 513 1065 2394 3008 513 Size: 552 1329 614 3074 Fragments arranged by size: 3074 1329 614 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2153 2497 3596 3613 3766 3951 4396 Size: 1255 573 344 1099 17 153 185 445 Cuts at: 4396 325 Size: 1498 Fragments arranged by size: 1498 1255 1099 573 445 344 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3815 5527 96 Size: 274 80 33 51 1443 1838 1712 138 Fragments arranged by size: 1838 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2675 2707 5529 5561 2675 Size: 32 2822 32 2683 Fragments arranged by size: 2822 2683 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5569 XbaI T'CTAG_A Cuts at: 2905 2905 Size: 5569 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2022 2094 2022 Size: 72 5497 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5569 XmaI C'CCGG_G Cuts at: 1870 2583 2894 1870 Size: 713 311 4545 Fragments arranged by size: 4545 713 311 XmnI GAAnn'nnTTC Cuts at: 3698 3698 Size: 5569 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3377 276 Size: 53 83 186 936 1843 2468 Fragments arranged by size: 2468 1843 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI