################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbsI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I CelII ClaI CpoI DpnII DraI DrdI Eam1105I EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HindIII HpaI KpnI MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NdeI NdeII NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI Sau3AI ScaI SexAI SfaNI SfuI SmlI SnaBI SpeI SphI SspBI SspI SwaI TfiI Tru9I Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 301 GTCTAC GT^[AC][GT]AC AlwNI 11 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 403 AGCGCT AGC^GCT ApaI 516 GGGCCC GGGCC^C Asp700I 381 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbrPI 252 CACGTG CAC^GTG BfaI 298 CTAG C^TAG BsaAI 252 CACGTG [CT]AC^GT[GA] BsaI 440 GGTCTC GGTCTC(1/5) BsgI 339 CTGCAC GTGCAG(16/14) BsmBI 150 CGTCTC CGTCTC(1/5) BsmFI 3 GGGAC GGGAC(10/14) BspMI 230 ACCTGC ACCTGC(4/8) BsrBI 324 CCGCTC CCGCTC(-3/-3) BsrI 477 CCAGT ACTGG(1/-1) DraIII 135 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 403 AGCGCT AGC^GCT EcoNI 375 CCTGGCGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 263 CCATGG C^C[AT][AT]GG HincII 145 GTCAAC GT[CT]^[GA]AC HindII 145 GTCAAC GT[CT]^[GA]AC HinfI 131 GAGTC G^A[ACGT]TC HphI 396 GGTGA GGTGA(8/7) MaeI 298 CTAG C^TAG MaeIII 133 GTCAC ^GT[ACGT]AC NaeI 283 GCCGGC GCC^GGC NcoI 263 CCATGG C^CATGG NgoMIV 283 GCCGGC G^CCGGC PleI 131 GAGTC GAGTC(4/5) PmaCI 252 CACGTG CAC^GTG PmlI 252 CACGTG CAC^GTG PpuMI 227 AGGACCT [GA]G^G[AT]CC[CT] PstI 487 CTGCAG CTGCA^G PvuII 115 CAGCTG CAG^CTG SapI 399 GAAGAGC GCTCTTC(1/4) SbfI 486 CCTGCAGG CCTGCA^GG SfcI 487 CTGCAG C^T[GA][CT]AG SfiI 465 GGCCCTCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 282 CGCCGGCG C[GA]^CCGG[CT]G SmaI 429 CCCGGG CCC^GGG Sse8387I 486 CCTGCAGG CCTGCA^GG StuI 383 AGGCCT AGG^CCT StyI 263 CCATGG C^C[AT][AT]GG TaqI 212 TCGA T^CGA Tsp45I 133 GTCAC ^GT[GC]AC TthHB8I 212 TCGA T^CGA XmaCI 429 CCCGGG C^CCGGG XmaI 429 CCCGGG C^CCGGG XmnI 381 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AcyI 20 GGCGCC G[GA]^CG[CT]C 289 GGCGCC AfaI 43 GTAC GT^AC 348 GTAC AocI 506 CCTTAGG CC^T[ACGT]AGG 520 CCTGAGG AvaI 82 CTCGGG C^[CT]CG[GA]G 429 CCCGGG AvaII 4 GGACC G^G[AT]CC 228 GGACC BbeI 20 GGCGCC GGCGC^C 289 GGCGCC BbvCI 369 CCTCAGC CCTCAGC(-5/-2) 223 GCTGAGG BglI 262 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 466 GCCCTCCAGGC BsaHI 20 GGCGCC G[GA]^CG[CT]C 289 GGCGCC BseRI 109 GAGGAG GAGGAG(10/8) 460 GAGGAG BsiEI 315 CGGCCG CG[GA][CT]^CG 329 CGGCCG BsmAI 151 GTCTC GTCTC(1/5) 441 GTCTC BsoBI 82 CTCGGG C^[CT]CG[GA]G 429 CCCGGG BsrFI 6 ACCGGC [GA]^CCGG[CT] 283 GCCGGC Bsu36I 506 CCTTAGG CC^T[ACGT]AGG 520 CCTGAGG Cfr10I 6 ACCGGC [GA]^CCGG[CT] 283 GCCGGC EaeI 315 CGGCCG [CT]^GGCC[GA] 329 CGGCCG EagI 315 CGGCCG C^GGCCG 329 CGGCCG EarI 76 CTCTTC CTCTTC(1/4) 399 GAAGAG EclXI 315 CGGCCG C^GGCCG 329 CGGCCG Eco52I 315 CGGCCG C^GGCCG 329 CGGCCG Eco81I 506 CCTTAGG CC^T[ACGT]AGG 520 CCTGAGG HgaI 62 GCGTC GACGC(5/10) 143 GCGTC Hin1I 20 GGCGCC G[GA]^CG[CT]C 289 GGCGCC KasI 20 GGCGCC G^GCGCC 289 GGCGCC Ksp632I 76 CTCTTC CTCTTC(1/4) 399 GAAGAG MaeII 149 ACGT A^CGT 253 ACGT MboII 399 GAAGA GAAGA(8/7) 77 TCTTC NarI 20 GGCGCC GG^CGCC 289 GGCGCC NlaIII 264 CATG CATG^ 354 CATG RsaI 43 GTAC GT^AC 348 GTAC SfoI 20 GGCGCC GGC^GCC 289 GGCGCC ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AccII AciI AluI BanI BanII BbvI BcnI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BssHII BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII DsaI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI KspI MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV SacII Sau96I ScrFI TseI