(Circular) (Six-base) MAPSORT of: pFN21AE5074.seq Check: 3460 from: 1 to: 5233 pFN21AE5074 5233 bp With 182 enzymes: SgfI * December 8, 2014 13:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3043 278 Size: 53 83 186 936 1507 2468 Fragments arranged by size: 2468 1507 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2307 2576 1937 Size: 370 269 4594 Fragments arranged by size: 4594 370 269 Acc65I G'GTAC_C Cuts at: 2554 2554 Size: 5233 AclI AA'CG_TT Cuts at: 1563 3360 3733 1563 Size: 1797 373 3063 Fragments arranged by size: 3063 1797 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3296 4680 4948 1632 Size: 1664 1384 268 1917 Fragments arranged by size: 1917 1664 1384 268 AfeI AGC'GCT Cuts at: 2410 2410 Size: 5233 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5036 AflIII A'CryG_T Cuts at: 1203 4132 5109 1203 Size: 2929 977 1327 Fragments arranged by size: 2929 1327 977 AgeI A'CCGG_T Cuts at: 4064 4064 Size: 5233 AhdI GACnn_n'nnGTC Cuts at: 3962 3962 Size: 5233 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5233 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5233 AlwNI CAG_nnn'CTG Cuts at: 2021 2976 4548 2021 Size: 955 1572 2706 Fragments arranged by size: 2706 1572 955 ApaI G_GGCC'C Cuts at: 2525 2525 Size: 5233 ApaLI G'TGCA_C Cuts at: 3290 4446 3290 Size: 1156 4077 ApoI r'AATT_y Cuts at: 1493 2542 2768 2920 5029 1493 Size: 1049 226 152 2109 1697 Fragments arranged by size: 2109 1697 1049 226 152 AseI AT'TA_AT Cuts at: 160 1032 3787 160 Size: 872 2755 1606 Fragments arranged by size: 2755 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5233 AvaI C'yCGr_G Cuts at: 1870 1943 2087 2434 2558 1870 Size: 73 144 347 124 4545 Fragments arranged by size: 4545 347 144 124 73 BamHI G'GATC_C Cuts at: 2563 2930 2563 Size: 367 4866 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2025 2294 2364 2554 4009 Size: 325 217 627 238 269 70 190 1455 Cuts at: 4009 618 Size: 1842 Fragments arranged by size: 1842 1455 627 325 269 238 217 190 70 BanII G_rGCy'C Cuts at: 1391 2256 2505 2525 2552 1391 Size: 865 249 20 27 4072 Fragments arranged by size: 4072 865 249 27 20 BbeI G_GCGC'C Cuts at: 2029 2298 2029 Size: 269 4964 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4262 BbvCI CC'TCA_GC Cuts at: 2229 2375 2229 Size: 146 5087 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3424 3458 1903 Size: 34 1487 34 3678 Fragments arranged by size: 3678 1487 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3125 4335 3125 Size: 1210 4023 BclI T'GATC_A Cuts at: 1548 1548 Size: 5233 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2273 2477 3844 Size: 107 122 71 631 605 601 204 1367 Cuts at: 3844 136 Size: 1525 Fragments arranged by size: 1525 1367 631 605 601 204 122 107 71 BglII A'GATC_T Cuts at: 5228 5228 Size: 5233 BlpI GC'TnA_GC Cuts at: 2650 2650 Size: 5233 Bme1580I G_kGCm'C Cuts at: 1790 2211 2525 3294 4450 1790 Size: 421 314 769 1156 2573 Fragments arranged by size: 2573 1156 769 421 314 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5233 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2476 2964 3922 Size: 416 53 83 399 24 1059 488 958 Cuts at: 3922 442 Size: 1753 Fragments arranged by size: 1753 1059 958 488 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5233 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2270 2438 2459 3893 1239 Size: 1031 168 21 1434 2579 Fragments arranged by size: 2579 1434 1031 168 21 Bpu10I CC'TnA_GC Cuts at: 2229 2375 2229 Size: 146 5087 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3358 4223 4521 3358 Size: 865 298 4070 Fragments arranged by size: 4070 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2451 3896 882 Size: 617 952 1445 2219 Fragments arranged by size: 2219 1445 952 617 BsaAI yAC'GTr Cuts at: 493 1204 2259 3024 493 Size: 711 1055 765 2702 Fragments arranged by size: 2702 1055 765 711 BsaBI GATnn'nnATC Cuts at: 5227 5227 Size: 5233 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2026 2295 3040 3422 Size: 53 83 186 936 493 269 745 382 Cuts at: 3422 4057 275 Size: 635 1451 Fragments arranged by size: 1451 936 745 635 493 382 269 186 83 53 BsaWI w'CCGG_w Cuts at: 3666 4064 4338 4485 4896 3666 Size: 398 274 147 411 4003 Fragments arranged by size: 4003 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2243 2273 1178 Size: 30 165 30 840 30 4138 Fragments arranged by size: 4138 840 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2129 2480 1186 Size: 943 351 3939 Fragments arranged by size: 3939 943 351 BseYI C'CCAG_C Cuts at: 2021 2506 4436 4932 2021 Size: 485 1930 496 2322 Fragments arranged by size: 2322 1930 496 485 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2329 1163 Size: 573 593 4067 Fragments arranged by size: 4067 593 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2323 2337 2683 3444 4472 1540 Size: 275 186 322 14 346 761 1028 2301 Fragments arranged by size: 2301 1028 761 346 322 275 186 14 BsiHKAI G_wGCw'C Cuts at: 3294 3379 4450 3294 Size: 85 1071 4077 Fragments arranged by size: 4077 1071 85 BsmI GAATG_Cn' Cuts at: 2744 2837 2744 Size: 93 5140 BsmBI CGTCTCn'nnnn_ Cuts at: 2161 2161 Size: 5233 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2211 2256 2505 2525 2552 3294 3379 Size: 399 421 45 249 20 27 742 85 Cuts at: 3379 4450 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 421 399 249 85 45 27 20 BspEI T'CCGG_A Cuts at: 4896 4896 Size: 5233 BspHI T'CATG_A Cuts at: 3122 4852 3122 Size: 1730 3503 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2244 2588 844 Size: 1400 344 3489 Fragments arranged by size: 3489 1400 344 BsrBI CCG'CTC Cuts at: 1394 1823 2331 3120 1394 Size: 429 508 789 3507 Fragments arranged by size: 3507 789 508 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3728 3902 65 Size: 1183 2480 174 1396 Fragments arranged by size: 2480 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2011 2288 3877 4064 1700 Size: 110 201 277 1589 187 2869 Fragments arranged by size: 2869 1589 277 201 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5233 BssHII G'CGCG_C Cuts at: 1925 2094 2126 2128 2179 2181 2284 1925 Size: 169 32 2 51 2 103 4874 Fragments arranged by size: 4874 169 103 51 32 2 2 BssSI C'ACGA_G Cuts at: 3293 4305 3293 Size: 1012 4221 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5233 BstBI TT'CG_AA Cuts at: 4037 4037 Size: 5233 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5233 BstYI r'GATC_y Cuts at: 1972 2563 2930 3315 3332 4784 4870 4882 5228 Size: 591 367 385 17 1452 86 12 346 Cuts at: 5228 1972 Size: 1977 Fragments arranged by size: 1977 1452 591 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2512 2526 2512 Size: 14 5219 BtgI C'CryG_G Cuts at: 513 1065 2004 2029 2103 2268 2277 2368 513 Size: 552 939 25 74 165 9 91 3378 Fragments arranged by size: 3378 939 552 165 91 74 25 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4494 Fragments arranged by size: 4494 683 56 BtsI GCAGTG_nn' Cuts at: 2749 3543 3563 2749 Size: 794 20 4419 Fragments arranged by size: 4419 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5233 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5198 DraI TTT'AAA Cuts at: 2537 2537 Size: 5233 DraIII CAC_nnn'GTG Cuts at: 2145 2145 Size: 5233 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4240 809 Size: 507 2924 1802 Fragments arranged by size: 2924 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2320 2334 2680 Size: 54 1425 50 275 18 490 14 346 Cuts at: 2680 3569 5198 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 490 346 275 54 50 43 18 14 EagI C'GGCC_G Cuts at: 1537 1812 2320 2334 2680 1537 Size: 275 508 14 346 4090 Fragments arranged by size: 4090 508 346 275 14 EarI CTCTTCn'nnn_ Cuts at: 1353 2087 2399 3163 1353 Size: 734 312 764 3423 Fragments arranged by size: 3423 764 734 312 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3820 4194 4340 387 Size: 1064 138 2231 374 146 1280 Fragments arranged by size: 2231 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2270 2438 2459 3296 3893 4680 4948 Size: 393 638 168 21 837 597 787 268 Cuts at: 4948 1239 Size: 1524 Fragments arranged by size: 1524 837 787 638 597 393 268 168 21 EcoNI CCTnn'n_nnAGG Cuts at: 2384 2384 Size: 5233 EcoO109I rG'GnC_Cy Cuts at: 2233 2470 2522 2233 Size: 237 52 4944 Fragments arranged by size: 4944 237 52 EcoRI G'AATT_C Cuts at: 2542 2920 2542 Size: 378 4855 FspI TGC'GCA Cuts at: 3739 3739 Size: 5233 HaeII r_GCGC'y Cuts at: 1678 2029 2298 2412 4380 1678 Size: 351 269 114 1968 2531 Fragments arranged by size: 2531 1968 351 269 114 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3874 3906 3948 3980 1373 Size: 32 135 32 2302 32 42 32 2626 Fragments arranged by size: 2626 2302 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2152 2577 2823 669 Size: 988 281 214 425 246 3079 Fragments arranged by size: 3079 988 425 281 246 214 HindIII A'AGCT_T Cuts at: 748 748 Size: 5233 HpaI GTT'AAC Cuts at: 2823 2823 Size: 5233 KasI G'GCGC_C Cuts at: 2025 2294 2025 Size: 269 4964 KpnI G_GTAC'C Cuts at: 2558 2558 Size: 5233 MfeI C'AATT_G Cuts at: 2832 2832 Size: 5233 MluI A'CGCG_T Cuts at: 5109 5109 Size: 5233 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4347 4531 1305 Size: 3042 184 2007 Fragments arranged by size: 3042 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3411 Fragments arranged by size: 3411 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3191 3550 3709 518 Size: 648 2025 359 159 2042 Fragments arranged by size: 2042 2025 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2031 2105 2122 2279 2370 2649 3326 4474 Size: 425 74 17 157 91 279 677 1148 Cuts at: 4474 4719 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 425 279 245 157 91 74 17 NaeI GCC'GGC Cuts at: 2290 2290 Size: 5233 NarI GG'CG_CC Cuts at: 2026 2295 2026 Size: 269 4964 NcoI C'CATG_G Cuts at: 513 1065 2004 2268 513 Size: 552 939 264 3478 Fragments arranged by size: 3478 939 552 264 NdeI CA'TA_TG Cuts at: 387 387 Size: 5233 NgoMIV G'CCGG_C Cuts at: 2288 2288 Size: 5233 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5233 NotI GC'GGCC_GC Cuts at: 2680 2680 Size: 5233 NspI r_CATG'y Cuts at: 1132 1229 2591 4136 1132 Size: 97 1362 1545 2229 Fragments arranged by size: 2229 1545 1362 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5233 PciI A'CATG_T Cuts at: 4132 4132 Size: 5233 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5233 PmeI GTTT'AAAC Cuts at: 2537 2537 Size: 5233 PmlI CAC'GTG Cuts at: 2259 2259 Size: 5233 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3302 3334 4815 4847 3302 Size: 32 1481 32 3688 Fragments arranged by size: 3688 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2233 2233 Size: 5233 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5233 PsiI TTA'TAA Cuts at: 2803 2803 Size: 5233 PspOMI G'GGCC_C Cuts at: 2521 2521 Size: 5233 PstI C_TGCA'G Cuts at: 830 2496 2585 830 Size: 1666 89 3478 Fragments arranged by size: 3478 1666 89 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5233 PvuII CAG'CTG Cuts at: 2122 2122 Size: 5233 SacII CC_GC'GG Cuts at: 2032 2106 2280 2371 2032 Size: 74 174 91 4894 Fragments arranged by size: 4894 174 91 74 SalI G'TCGA_C Cuts at: 1936 2575 1936 Size: 639 4594 SapI GCTCTTCn'nnn_ Cuts at: 2399 2399 Size: 5233 SbfI CC_TGCA'GG Cuts at: 2496 2585 2496 Size: 89 5144 ScaI AGT'ACT Cuts at: 3481 5193 3481 Size: 1712 3521 SfcI C'TryA_G Cuts at: 826 1045 2492 2581 3716 4397 4588 826 Size: 219 1447 89 1135 681 191 1471 Fragments arranged by size: 1471 1447 1135 681 219 191 89 SfiI GGCCn_nnn'nGGCC Cuts at: 2477 2477 Size: 5233 SfoI GGC'GCC Cuts at: 2027 2296 2027 Size: 269 4964 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5233 SgrAI Cr'CCGG_yG Cuts at: 1700 2288 1700 Size: 588 4645 SmaI CCC'GGG Cuts at: 1872 2436 2560 1872 Size: 564 124 4545 Fragments arranged by size: 4545 564 124 SmlI C'TyrA_G Cuts at: 820 1017 1943 3337 4238 4500 820 Size: 197 926 1394 901 262 1553 Fragments arranged by size: 1553 1394 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3024 493 Size: 2531 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5233 SphI G_CATG'C Cuts at: 1132 2591 1132 Size: 1459 3774 SspI AAT'ATT Cuts at: 5 52 3157 5 Size: 47 3105 2081 Fragments arranged by size: 3105 2081 47 StuI AGG'CCT Cuts at: 1517 2390 1517 Size: 873 4360 StyI C'CwwG_G Cuts at: 513 1065 2004 2268 2672 513 Size: 552 939 264 404 3074 Fragments arranged by size: 3074 939 552 404 264 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3260 3277 3430 3615 4060 325 Size: 1255 1680 17 153 185 445 1498 Fragments arranged by size: 1680 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3479 5191 96 Size: 274 80 33 51 1443 1502 1712 138 Fragments arranged by size: 1712 1502 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2243 2275 5193 5225 2243 Size: 32 2918 32 2251 Fragments arranged by size: 2918 2251 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5233 XbaI T'CTAG_A Cuts at: 2569 2569 Size: 5233 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5233 XmaI C'CCGG_G Cuts at: 1870 2434 2558 1870 Size: 564 124 4545 Fragments arranged by size: 4545 564 124 XmnI GAAnn'nnTTC Cuts at: 2390 3362 2390 Size: 972 4261 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3041 276 Size: 53 83 186 936 1507 2468 Fragments arranged by size: 2468 1507 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SanDI SexAI SrfI SwaI XcmI