(Linear) (Six-base) MAPSORT of: KIEE5074.seq Check: 6386 from: 1 to: 528 KIEE5074.orf With 182 enzymes: SgfI * December 5, 2014 12:59 .. AccI GT'mk_AC Cuts at: 0 302 528 Size: 302 226 AfeI AGC'GCT Cuts at: 0 405 528 Size: 405 123 AlwNI CAG_nnn'CTG Cuts at: 0 16 528 Size: 16 512 ApaI G_GGCC'C Cuts at: 0 520 528 Size: 520 8 AvaI C'yCGr_G Cuts at: 0 82 429 528 Size: 82 347 99 Fragments arranged by size: 347 99 82 BanI G'GyrC_C Cuts at: 0 20 289 359 528 Size: 20 269 70 169 Fragments arranged by size: 269 169 70 20 BanII G_rGCy'C Cuts at: 0 251 500 520 528 Size: 251 249 20 8 Fragments arranged by size: 251 249 20 8 BbeI G_GCGC'C Cuts at: 0 24 293 528 Size: 24 269 235 Fragments arranged by size: 269 235 24 BbvCI CC'TCA_GC Cuts at: 0 224 370 528 Size: 224 146 158 Fragments arranged by size: 224 158 146 BglI GCCn_nnn'nGGC Cuts at: 0 268 472 528 Size: 268 204 56 Fragments arranged by size: 268 204 56 Bme1580I G_kGCm'C Cuts at: 0 206 520 528 Size: 206 314 8 Fragments arranged by size: 314 206 8 BmrI ACTGGGnnnn_n' Cuts at: 0 471 528 Size: 471 57 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 265 433 454 528 Size: 265 168 21 74 Fragments arranged by size: 265 168 74 21 Bpu10I CC'TnA_GC Cuts at: 0 224 370 528 Size: 224 146 158 Fragments arranged by size: 224 158 146 BsaI GGTCTCn'nnnn_ Cuts at: 0 446 528 Size: 446 82 BsaAI yAC'GTr Cuts at: 0 254 528 Size: 254 274 BsaHI Gr'CG_yC Cuts at: 0 21 290 528 Size: 21 269 238 Fragments arranged by size: 269 238 21 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 238 268 528 Size: 238 30 260 Fragments arranged by size: 260 238 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 124 475 528 Size: 124 351 53 Fragments arranged by size: 351 124 53 BseYI C'CCAG_C Cuts at: 0 16 501 528 Size: 16 485 27 Fragments arranged by size: 485 27 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 324 528 Size: 324 204 BsiEI CG_ry'CG Cuts at: 0 318 332 528 Size: 318 14 196 Fragments arranged by size: 318 196 14 BsmBI CGTCTCn'nnnn_ Cuts at: 0 156 528 Size: 156 372 Bsp1286I G_dGCh'C Cuts at: 0 206 251 500 520 528 Size: 206 45 249 20 8 Fragments arranged by size: 249 206 45 20 8 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 239 528 Size: 239 289 BsrBI CCG'CTC Cuts at: 0 326 528 Size: 326 202 BsrFI r'CCGG_y Cuts at: 0 6 283 528 Size: 6 277 245 Fragments arranged by size: 277 245 6 BssHII G'CGCG_C Cuts at: 0 89 121 123 174 176 279 528 Size: 89 32 2 51 2 103 249 Fragments arranged by size: 249 103 89 51 32 2 2 Bsu36I CC'TnA_GG Cuts at: 0 507 521 528 Size: 507 14 7 Fragments arranged by size: 507 14 7 BtgI C'CryG_G Cuts at: 0 24 98 263 272 363 528 Size: 24 74 165 9 91 165 Fragments arranged by size: 165 165 91 74 24 9 DraIII CAC_nnn'GTG Cuts at: 0 140 528 Size: 140 388 EaeI y'GGCC_r Cuts at: 0 315 329 528 Size: 315 14 199 Fragments arranged by size: 315 199 14 EagI C'GGCC_G Cuts at: 0 315 329 528 Size: 315 14 199 Fragments arranged by size: 315 199 14 EarI CTCTTCn'nnn_ Cuts at: 0 82 394 528 Size: 82 312 134 Fragments arranged by size: 312 134 82 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 265 433 454 528 Size: 265 168 21 74 Fragments arranged by size: 265 168 74 21 EcoNI CCTnn'n_nnAGG Cuts at: 0 379 528 Size: 379 149 EcoO109I rG'GnC_Cy Cuts at: 0 228 465 517 528 Size: 228 237 52 11 Fragments arranged by size: 237 228 52 11 HaeII r_GCGC'y Cuts at: 0 24 293 407 528 Size: 24 269 114 121 Fragments arranged by size: 269 121 114 24 HincII GTy'rAC Cuts at: 0 147 528 Size: 147 381 KasI G'GCGC_C Cuts at: 0 20 289 528 Size: 20 269 239 Fragments arranged by size: 269 239 20 MspA1I CmG'CkG Cuts at: 0 26 100 117 274 365 528 Size: 26 74 17 157 91 163 Fragments arranged by size: 163 157 91 74 26 17 NaeI GCC'GGC Cuts at: 0 285 528 Size: 285 243 NarI GG'CG_CC Cuts at: 0 21 290 528 Size: 21 269 238 Fragments arranged by size: 269 238 21 NcoI C'CATG_G Cuts at: 0 263 528 Size: 263 265 NgoMIV G'CCGG_C Cuts at: 0 283 528 Size: 283 245 PmlI CAC'GTG Cuts at: 0 254 528 Size: 254 274 PpuMI rG'GwC_Cy Cuts at: 0 228 528 Size: 228 300 PspOMI G'GGCC_C Cuts at: 0 516 528 Size: 516 12 PstI C_TGCA'G Cuts at: 0 491 528 Size: 491 37 PvuII CAG'CTG Cuts at: 0 117 528 Size: 117 411 SacII CC_GC'GG Cuts at: 0 27 101 275 366 528 Size: 27 74 174 91 162 Fragments arranged by size: 174 162 91 74 27 SapI GCTCTTCn'nnn_ Cuts at: 0 394 528 Size: 394 134 SbfI CC_TGCA'GG Cuts at: 0 491 528 Size: 491 37 SfcI C'TryA_G Cuts at: 0 487 528 Size: 487 41 SfiI GGCCn_nnn'nGGCC Cuts at: 0 472 528 Size: 472 56 SfoI GGC'GCC Cuts at: 0 22 291 528 Size: 22 269 237 Fragments arranged by size: 269 237 22 SgrAI Cr'CCGG_yG Cuts at: 0 283 528 Size: 283 245 SmaI CCC'GGG Cuts at: 0 431 528 Size: 431 97 StuI AGG'CCT Cuts at: 0 385 528 Size: 385 143 StyI C'CwwG_G Cuts at: 0 263 528 Size: 263 265 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 238 270 528 Size: 238 32 258 Fragments arranged by size: 258 238 32 XmaI C'CCGG_G Cuts at: 0 429 528 Size: 429 99 XmnI GAAnn'nnTTC Cuts at: 0 385 528 Size: 385 143 Enzymes that do cut: AccI AfeI AlwNI ApaI AvaI BanI BanII BbeI BbvCI BglI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII Bsu36I BtgI DraIII EaeI EagI EarI Eco57MI EcoNI EcoO109I HaeII HincII KasI MspA1I NaeI NarI NcoI NgoMIV PmlI PpuMI PspOMI PstI PvuII SacII SapI SbfI SfcI SfiI SfoI SgrAI SmaI StuI StyI TstI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI BpuEI BsaBI BsaWI BsiHKAI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgZI BtsI ClaI CspCI DraI DrdI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI KpnI MfeI MluI MmeI MscI MslI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI ScaI SexAI SgfI SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI Tth111I XbaI XcmI XhoI ZraI