################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BfrI BglI BlnI BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 21 enzymes) # ################################### AcyI 163 GACGCC G[GA]^CG[CT]C AfaI 530 GTAC GT^AC BanI 1122 GGTGCC G^G[CT][GA]CC BbvCI 1345 CCTCAGC CCTCAGC(-5/-2) BsaHI 163 GACGCC G[GA]^CG[CT]C BsgI 103 CTGCAC GTGCAG(16/14) BsiEI 807 CGACCG CG[GA][CT]^CG BspHI 143 TCATGA T^CATGA BsrBI 712 GAGCGG CCGCTC(-3/-3) DraI 961 TTTAAA TTT^AAA DsaI 1727 CCGTGG C^C[GA][CT]GG EcoRI 1172 GAATTC G^AATTC Hin1I 163 GACGCC G[GA]^CG[CT]C PflMI 1836 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 866 GGGACCT [GA]G^G[AT]CC[CT] PvuII 1765 CAGCTG CAG^CTG RcaI 143 TCATGA T^CATGA RsaI 530 GTAC GT^AC SacI 1747 GAGCTC GAGCT^C Van91I 1836 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1046 CCAAAAATTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 43 enzymes) # ################################### AlwI 1043 GATCC GGATC(4/5) 1712 GGATC AlwNI 983 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG 1573 CAGACCCTG AspHI 856 GAGCAC G[AT]GC[AT]^C 1747 GAGCTC AvaI 665 CCCGGG C^[CT]CG[GA]G 1208 CCCGGG BalI 266 TGGCCA TGG^CCA 1104 TGGCCA BanII 1732 GAGCCC G[GA]GC[CT]^C 1747 GAGCTC BbsI 568 GAAGAC GAAGAC(2/6) 1627 GAAGAC BclI 1320 TGATCA T^GATCA 1769 TGATCA BglII 433 AGATCT A^GATCT 1790 AGATCT BlpI 78 GCTGAGC GC^T[ACGT]AGC 1744 GCTGAGC Bpu1102I 78 GCTGAGC GC^T[ACGT]AGC 1744 GCTGAGC BpuAI 568 GAAGAC GAAGAC(2/6) 1627 GAAGAC BseRI 503 GAGGAG GAGGAG(10/8) 1402 GAGGAG BsiHKAI 856 GAGCAC G[AT]GC[AT]^C 1747 GAGCTC BsmBI 506 GAGACG CGTCTC(1/5) 1438 GAGACG BsoBI 665 CCCGGG C^[CT]CG[GA]G 1208 CCCGGG BspMI 101 ACCTGC ACCTGC(4/8) 1098 ACCTGC BsrI 136 ACTGG ACTGG(1/-1) 1073 CCAGT BstXI 227 CCATAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 324 CCAGATAATTGG BstYI 433 AGATCT [GA]^GATC[CT] 1790 AGATCT CelII 78 GCTGAGC GC^T[ACGT]AGC 1744 GCTGAGC EaeI 266 TGGCCA [CT]^GGCC[GA] 1104 TGGCCA EcoNI 216 CCTACAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1695 CCTGCAGCAGG FauI 289 GCGGG CCCGC(4/6) 714 GCGGG FbaI 1320 TGATCA T^GATCA 1769 TGATCA HaeII 33 GGCGCT [GA]GCGC^[CT] 1236 GGCGCT MflI 433 AGATCT [GA]^GATC[CT] 1790 AGATCT MluNI 266 TGGCCA TGG^CCA 1104 TGGCCA MscI 266 TGGCCA TGG^CCA 1104 TGGCCA MseI 962 TTAA T^TAA 1738 TTAA MslI 117 CACGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 228 CATAGACGTG MspA1I 61 CCGCTG C[AC]G^C[GT]G 1765 CAGCTG PstI 1654 CTGCAG CTGCA^G 1696 CTGCAG SfcI 1654 CTGCAG C^T[GA][CT]AG 1696 CTGCAG SmaI 665 CCCGGG CCC^GGG 1208 CCCGGG SmlI 429 CTCAAG C^T[CT][GA]AG 1816 CTCAAG TaqI 543 TCGA T^CGA 1867 TCGA TfiI 48 GATTC G^A[AT]TC 281 GAATC Tru9I 962 TTAA T^TAA 1738 TTAA TthHB8I 543 TCGA T^CGA 1867 TCGA XhoII 433 AGATCT [GA]^GATC[CT] 1790 AGATCT XmaCI 665 CCCGGG C^CCGGG 1208 CCCGGG XmaI 665 CCCGGG C^CCGGG 1208 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AccII AciI AcsI AluI ApoI AvaII BbvI BcnI BfaI BmyI BpmI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI StyI TseI Tsp45I Tsp509I TspRI