(Circular) (Six-base) MAPSORT of: pFN21AE2054.seq Check: 4274 from: 1 to: 6580 pFN21AE2054 6580 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4390 278 Size: 53 83 186 936 2854 2468 Fragments arranged by size: 2854 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3923 1937 Size: 1986 4594 Acc65I G'GTAC_C Cuts at: 3901 3901 Size: 6580 AclI AA'CG_TT Cuts at: 1563 4707 5080 1563 Size: 3144 373 3063 Fragments arranged by size: 3144 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4643 6027 6295 1632 Size: 3011 1384 268 1917 Fragments arranged by size: 3011 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6383 AflIII A'CryG_T Cuts at: 1203 5479 6456 1203 Size: 4276 977 1327 Fragments arranged by size: 4276 1327 977 AgeI A'CCGG_T Cuts at: 5411 5411 Size: 6580 AhdI GACnn_n'nnGTC Cuts at: 5309 5309 Size: 6580 AleI CACnn'nnGTG Cuts at: 1166 2126 1166 Size: 960 5620 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6580 AlwNI CAG_nnn'CTG Cuts at: 2993 3583 4323 5895 2993 Size: 590 740 1572 3678 Fragments arranged by size: 3678 1572 740 590 ApaLI G'TGCA_C Cuts at: 4637 5793 4637 Size: 1156 5424 ApoI r'AATT_y Cuts at: 1493 3055 3144 3177 3435 3889 4115 4267 6376 Size: 1562 89 33 258 454 226 152 2109 Cuts at: 6376 1493 Size: 1697 Fragments arranged by size: 2109 1697 1562 454 258 226 152 89 33 AseI AT'TA_AT Cuts at: 160 1032 5134 160 Size: 872 4102 1606 Fragments arranged by size: 4102 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6580 AvaI C'yCGr_G Cuts at: 1870 1943 2670 3213 3905 1870 Size: 73 727 543 692 4545 Fragments arranged by size: 4545 727 692 543 73 BamHI G'GATC_C Cuts at: 3910 4277 3910 Size: 367 6213 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3127 3901 5356 618 Size: 325 217 627 1340 774 1455 1842 Fragments arranged by size: 1842 1455 1340 774 627 325 217 BanII G_rGCy'C Cuts at: 1391 3741 3756 3899 1391 Size: 2350 15 143 4072 Fragments arranged by size: 4072 2350 143 15 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2580 3639 928 Size: 971 681 1059 3869 Fragments arranged by size: 3869 1059 971 681 BbvCI CC'TCA_GC Cuts at: 3351 3351 Size: 6580 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4771 4805 1903 Size: 34 2834 34 3678 Fragments arranged by size: 3678 2834 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4472 5682 4472 Size: 1210 5370 BclI T'GATC_A Cuts at: 1548 3325 3774 1548 Size: 1777 449 4354 Fragments arranged by size: 4354 1777 449 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5191 136 Size: 107 122 71 631 605 3519 1525 Fragments arranged by size: 3519 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2438 3795 6575 2438 Size: 1357 2780 2443 Fragments arranged by size: 2780 2443 1357 BlpI GC'TnA_GC Cuts at: 2084 3750 3997 2084 Size: 1666 247 4667 Fragments arranged by size: 4667 1666 247 Bme1580I G_kGCm'C Cuts at: 1790 4641 5797 1790 Size: 2851 1156 2573 Fragments arranged by size: 2851 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2239 3570 3637 1318 Size: 921 1331 67 4261 Fragments arranged by size: 4261 1331 921 67 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4311 5269 442 Size: 416 53 83 399 24 2894 958 1753 Fragments arranged by size: 2894 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6580 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2312 2415 3123 3785 5240 1239 Size: 1073 103 708 662 1455 2579 Fragments arranged by size: 2579 1455 1073 708 662 103 Bpu10I CC'TnA_GC Cuts at: 2929 3351 2929 Size: 422 6158 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2419 3806 4705 5570 5868 2419 Size: 1387 899 865 298 3131 Fragments arranged by size: 3131 1387 899 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5243 882 Size: 617 3744 2219 Fragments arranged by size: 3744 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4371 493 Size: 711 3167 2702 Fragments arranged by size: 3167 2702 711 BsaBI GATnn'nnATC Cuts at: 6574 6574 Size: 6580 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2169 4387 4769 5404 Size: 53 83 186 936 636 2218 382 635 Cuts at: 5404 275 Size: 1451 Fragments arranged by size: 2218 1451 936 636 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5013 5411 5685 5832 6243 5013 Size: 398 274 147 411 5350 Fragments arranged by size: 5350 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2714 2744 1178 Size: 30 165 30 1311 30 5014 Fragments arranged by size: 5014 1311 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2523 3422 1186 Size: 1337 899 4344 Fragments arranged by size: 4344 1337 899 BseYI C'CCAG_C Cuts at: 2041 5783 6279 2041 Size: 3742 496 2342 Fragments arranged by size: 3742 2342 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2093 1163 Size: 573 357 5650 Fragments arranged by size: 5650 573 357 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2815 4030 4791 5819 1540 Size: 275 186 814 1215 761 1028 2301 Fragments arranged by size: 2301 1215 1028 814 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2865 3756 4641 4726 5797 2865 Size: 891 885 85 1071 3648 Fragments arranged by size: 3648 1071 891 885 85 BsmI GAATG_Cn' Cuts at: 4091 4184 4091 Size: 93 6487 BsmBI CGTCTCn'nnnn_ Cuts at: 2505 3437 2505 Size: 932 5648 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2865 3741 3756 3899 4641 4726 5797 Size: 399 1075 876 15 143 742 85 1071 Cuts at: 5797 1391 Size: 2174 Fragments arranged by size: 2174 1075 1071 876 742 399 143 85 15 BspEI T'CCGG_A Cuts at: 6243 6243 Size: 6580 BspHI T'CATG_A Cuts at: 2148 4469 6199 2148 Size: 2321 1730 2529 Fragments arranged by size: 2529 2321 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2115 3112 3935 844 Size: 1271 997 823 3489 Fragments arranged by size: 3489 1271 997 823 BsrBI CCG'CTC Cuts at: 1394 1823 2719 4467 1394 Size: 429 896 1748 3507 Fragments arranged by size: 3507 1748 896 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5075 5249 65 Size: 1183 3827 174 1396 Fragments arranged by size: 3827 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5224 5411 1700 Size: 110 3414 187 2869 Fragments arranged by size: 3414 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6580 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6580 BssSI C'ACGA_G Cuts at: 4640 5652 4640 Size: 1012 5568 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6580 BstBI TT'CG_AA Cuts at: 5384 5384 Size: 6580 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6580 BstXI CCAn_nnnn'nTGG Cuts at: 2239 2336 2239 Size: 97 6483 BstYI r'GATC_y Cuts at: 1972 2438 3795 3910 4277 4662 4679 6131 6217 Size: 466 1357 115 367 385 17 1452 86 Cuts at: 6217 6229 6575 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1357 466 385 367 346 115 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3732 513 Size: 552 2667 3361 Fragments arranged by size: 3361 2667 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5841 Fragments arranged by size: 5841 683 56 BtsI GCAGTG_nn' Cuts at: 3396 4096 4890 4910 3396 Size: 700 794 20 5066 Fragments arranged by size: 5066 794 700 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6580 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6545 DraI TTT'AAA Cuts at: 2968 3884 2968 Size: 916 5664 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5587 809 Size: 507 4271 1802 Fragments arranged by size: 4271 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2271 3109 4027 Size: 54 1425 50 275 18 441 838 918 Cuts at: 4027 4916 6545 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 918 889 838 441 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4027 1537 Size: 275 2215 4090 Fragments arranged by size: 4090 2215 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2469 3312 3399 4510 1353 Size: 1116 843 87 1111 3423 Fragments arranged by size: 3423 1116 1111 843 87 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2041 2643 5167 5541 5687 387 Size: 1064 138 452 602 2524 374 146 1280 Fragments arranged by size: 2524 1280 1064 602 452 374 146 138 EcoICRI GAG'CTC Cuts at: 3754 3754 Size: 6580 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2312 2415 3123 3785 4643 5240 6027 Size: 393 680 103 708 662 858 597 787 Cuts at: 6027 6295 1239 Size: 268 1524 Fragments arranged by size: 1524 858 787 708 680 662 597 393 268 103 EcoNI CCTnn'n_nnAGG Cuts at: 2225 3704 2225 Size: 1479 5101 EcoO109I rG'GnC_Cy Cuts at: 2155 2872 3697 2155 Size: 717 825 5038 Fragments arranged by size: 5038 825 717 EcoRI G'AATT_C Cuts at: 3177 3889 4267 3177 Size: 712 378 5490 Fragments arranged by size: 5490 712 378 FspI TGC'GCA Cuts at: 5086 5086 Size: 6580 HaeII r_GCGC'y Cuts at: 1678 2042 3245 5727 1678 Size: 364 1203 2482 2531 Fragments arranged by size: 2531 2482 1203 364 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2165 2197 2714 2746 2903 Size: 32 135 32 593 32 517 32 157 Cuts at: 2903 2935 3146 3178 5221 5253 5295 5327 1373 Size: 32 211 32 2043 32 42 32 2626 Fragments arranged by size: 2626 2043 593 517 211 157 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3924 4170 669 Size: 988 281 1986 246 3079 Fragments arranged by size: 3079 1986 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6580 HpaI GTT'AAC Cuts at: 4170 4170 Size: 6580 KpnI G_GTAC'C Cuts at: 3905 3905 Size: 6580 MfeI C'AATT_G Cuts at: 4179 4179 Size: 6580 MluI A'CGCG_T Cuts at: 6456 6456 Size: 6580 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3189 5694 5878 1305 Size: 1884 2505 184 2007 Fragments arranged by size: 2505 2007 1884 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2273 3111 10 Size: 54 1425 343 441 838 3479 Fragments arranged by size: 3479 1425 838 441 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2126 2237 4538 4897 5056 518 Size: 648 960 111 2301 359 159 2042 Fragments arranged by size: 2301 2042 960 648 359 159 111 MspA1I CmG'CkG Cuts at: 1606 2068 3772 3996 4673 5821 6066 1606 Size: 462 1704 224 677 1148 245 2120 Fragments arranged by size: 2120 1704 1148 677 462 245 224 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6028 NdeI CA'TA_TG Cuts at: 387 387 Size: 6580 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6580 NotI GC'GGCC_GC Cuts at: 4027 4027 Size: 6580 NspI r_CATG'y Cuts at: 1132 1229 3938 5483 1132 Size: 97 2709 1545 2229 Fragments arranged by size: 2709 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3604 1348 Size: 2256 4324 PciI A'CATG_T Cuts at: 5479 5479 Size: 6580 PflMI CCAn_nnn'nTGG Cuts at: 1108 3847 1108 Size: 2739 3841 PfoI T'CCnGG_A Cuts at: 3627 3627 Size: 6580 PmeI GTTT'AAAC Cuts at: 3884 3884 Size: 6580 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4649 4681 6162 6194 4649 Size: 32 1481 32 5035 Fragments arranged by size: 5035 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2872 2872 Size: 6580 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6580 PsiI TTA'TAA Cuts at: 4150 4150 Size: 6580 PstI C_TGCA'G Cuts at: 830 3663 3705 3932 830 Size: 2833 42 227 3478 Fragments arranged by size: 3478 2833 227 42 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6580 PvuII CAG'CTG Cuts at: 3772 3772 Size: 6580 SacI G_AGCT'C Cuts at: 3756 3756 Size: 6580 SalI G'TCGA_C Cuts at: 1936 3922 1936 Size: 1986 4594 SbfI CC_TGCA'GG Cuts at: 3932 3932 Size: 6580 ScaI AGT'ACT Cuts at: 4828 6540 4828 Size: 1712 4868 SfcI C'TryA_G Cuts at: 826 1045 3659 3701 3928 5063 5744 5935 826 Size: 219 2614 42 227 1135 681 191 1471 Fragments arranged by size: 2614 1471 1135 681 227 219 191 42 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6580 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6580 SmaI CCC'GGG Cuts at: 1872 2672 3215 3907 1872 Size: 800 543 692 4545 Fragments arranged by size: 4545 800 692 543 SmlI C'TyrA_G Cuts at: 820 1017 1943 2434 3821 4684 5585 5847 820 Size: 197 926 491 1387 863 901 262 1553 Fragments arranged by size: 1553 1387 926 901 863 491 262 197 SnaBI TAC'GTA Cuts at: 493 4371 493 Size: 3878 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6580 SphI G_CATG'C Cuts at: 1132 3938 1132 Size: 2806 3774 SrfI GCCC'GGGC Cuts at: 2672 2672 Size: 6580 SspI AAT'ATT Cuts at: 5 52 4504 5 Size: 47 4452 2081 Fragments arranged by size: 4452 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6580 StyI C'CwwG_G Cuts at: 513 1065 2616 2976 3546 4019 513 Size: 552 1551 360 570 473 3074 Fragments arranged by size: 3074 1551 570 552 473 360 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2817 3493 4607 4624 4777 4962 5407 Size: 1255 1237 676 1114 17 153 185 445 Cuts at: 5407 325 Size: 1498 Fragments arranged by size: 1498 1255 1237 1114 676 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2534 4826 6538 Size: 274 80 33 51 1443 557 2292 1712 Cuts at: 6538 96 Size: 138 Fragments arranged by size: 2292 1712 1443 557 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6540 6572 6540 Size: 32 6548 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6580 XbaI T'CTAG_A Cuts at: 3916 3916 Size: 6580 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3058 3058 Size: 6580 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6580 XmaI C'CCGG_G Cuts at: 1870 2670 3213 3905 1870 Size: 800 543 692 4545 Fragments arranged by size: 4545 800 692 543 XmnI GAAnn'nnTTC Cuts at: 4709 4709 Size: 6580 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4388 276 Size: 53 83 186 936 2854 2468 Fragments arranged by size: 2854 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SwaI