(Linear) (Six-base) MAPSORT of: KIEE2054.seq Check: 3234 from: 1 to: 1875 KIEE2054.orf With 182 enzymes: SgfI * July 15, 2010 18:54 .. AleI CACnn'nnGTG Cuts at: 0 121 1875 Size: 121 1754 AlwNI CAG_nnn'CTG Cuts at: 0 988 1578 1875 Size: 988 590 297 Fragments arranged by size: 988 590 297 ApoI r'AATT_y Cuts at: 0 1050 1139 1172 1430 1875 Size: 1050 89 33 258 445 Fragments arranged by size: 1050 445 258 89 33 AvaI C'yCGr_G Cuts at: 0 665 1208 1875 Size: 665 543 667 Fragments arranged by size: 667 665 543 BanI G'GyrC_C Cuts at: 0 1122 1875 Size: 1122 753 BanII G_rGCy'C Cuts at: 0 1736 1751 1875 Size: 1736 15 124 Fragments arranged by size: 1736 124 15 BbsI GAAGACnn'nnnn_ Cuts at: 0 575 1634 1875 Size: 575 1059 241 Fragments arranged by size: 1059 575 241 BbvCI CC'TCA_GC Cuts at: 0 1346 1875 Size: 1346 529 BclI T'GATC_A Cuts at: 0 1320 1769 1875 Size: 1320 449 106 Fragments arranged by size: 1320 449 106 BglII A'GATC_T Cuts at: 0 433 1790 1875 Size: 433 1357 85 Fragments arranged by size: 1357 433 85 BlpI GC'TnA_GC Cuts at: 0 79 1745 1875 Size: 79 1666 130 Fragments arranged by size: 1666 130 79 BmgBI CAC'GTC Cuts at: 0 234 1565 1632 1875 Size: 234 1331 67 243 Fragments arranged by size: 1331 243 234 67 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 307 410 1118 1780 1875 Size: 307 103 708 662 95 Fragments arranged by size: 708 662 307 103 95 Bpu10I CC'TnA_GC Cuts at: 0 924 1346 1875 Size: 924 422 529 Fragments arranged by size: 924 529 422 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 414 1801 1875 Size: 414 1387 74 Fragments arranged by size: 1387 414 74 BsaHI Gr'CG_yC Cuts at: 0 164 1875 Size: 164 1711 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 709 739 1875 Size: 709 30 1136 Fragments arranged by size: 1136 709 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 518 1417 1875 Size: 518 899 458 Fragments arranged by size: 899 518 458 BseYI C'CCAG_C Cuts at: 0 36 1875 Size: 36 1839 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 88 1875 Size: 88 1787 BsiEI CG_ry'CG Cuts at: 0 810 1875 Size: 810 1065 BsiHKAI G_wGCw'C Cuts at: 0 860 1751 1875 Size: 860 891 124 Fragments arranged by size: 891 860 124 BsmBI CGTCTCn'nnnn_ Cuts at: 0 500 1432 1875 Size: 500 932 443 Fragments arranged by size: 932 500 443 Bsp1286I G_dGCh'C Cuts at: 0 860 1736 1751 1875 Size: 860 876 15 124 Fragments arranged by size: 876 860 124 15 BspHI T'CATG_A Cuts at: 0 143 1875 Size: 143 1732 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 110 1107 1875 Size: 110 997 768 Fragments arranged by size: 997 768 110 BsrBI CCG'CTC Cuts at: 0 714 1875 Size: 714 1161 BstXI CCAn_nnnn'nTGG Cuts at: 0 234 331 1875 Size: 234 97 1544 Fragments arranged by size: 1544 234 97 BstYI r'GATC_y Cuts at: 0 433 1790 1875 Size: 433 1357 85 Fragments arranged by size: 1357 433 85 BtgI C'CryG_G Cuts at: 0 1727 1875 Size: 1727 148 BtsI GCAGTG_nn' Cuts at: 0 1391 1875 Size: 1391 484 DraI TTT'AAA Cuts at: 0 963 1875 Size: 963 912 EaeI y'GGCC_r Cuts at: 0 266 1104 1875 Size: 266 838 771 Fragments arranged by size: 838 771 266 EarI CTCTTCn'nnn_ Cuts at: 0 464 1307 1394 1875 Size: 464 843 87 481 Fragments arranged by size: 843 481 464 87 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 36 638 1875 Size: 36 602 1237 Fragments arranged by size: 1237 602 36 EcoICRI GAG'CTC Cuts at: 0 1749 1875 Size: 1749 126 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 307 410 1118 1780 1875 Size: 307 103 708 662 95 Fragments arranged by size: 708 662 307 103 95 EcoNI CCTnn'n_nnAGG Cuts at: 0 220 1699 1875 Size: 220 1479 176 Fragments arranged by size: 1479 220 176 EcoO109I rG'GnC_Cy Cuts at: 0 150 867 1692 1875 Size: 150 717 825 183 Fragments arranged by size: 825 717 183 150 EcoRI G'AATT_C Cuts at: 0 1172 1875 Size: 1172 703 HaeII r_GCGC'y Cuts at: 0 37 1240 1875 Size: 37 1203 635 Fragments arranged by size: 1203 635 37 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 160 192 709 741 898 930 1141 1173 Size: 160 32 517 32 157 32 211 32 Cuts at: 1173 1875 Size: 702 Fragments arranged by size: 702 517 211 160 157 32 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1184 1875 Size: 1184 691 MscI TGG'CCA Cuts at: 0 268 1106 1875 Size: 268 838 769 Fragments arranged by size: 838 769 268 MslI CAynn'nnrTG Cuts at: 0 121 232 1875 Size: 121 111 1643 Fragments arranged by size: 1643 121 111 MspA1I CmG'CkG Cuts at: 0 63 1767 1875 Size: 63 1704 108 Fragments arranged by size: 1704 108 63 PasI CC'CwG_GG Cuts at: 0 1599 1875 Size: 1599 276 PflMI CCAn_nnn'nTGG Cuts at: 0 1842 1875 Size: 1842 33 PfoI T'CCnGG_A Cuts at: 0 1622 1875 Size: 1622 253 PpuMI rG'GwC_Cy Cuts at: 0 867 1875 Size: 867 1008 PstI C_TGCA'G Cuts at: 0 1658 1700 1875 Size: 1658 42 175 Fragments arranged by size: 1658 175 42 PvuII CAG'CTG Cuts at: 0 1767 1875 Size: 1767 108 SacI G_AGCT'C Cuts at: 0 1751 1875 Size: 1751 124 SfcI C'TryA_G Cuts at: 0 1654 1696 1875 Size: 1654 42 179 Fragments arranged by size: 1654 179 42 SmaI CCC'GGG Cuts at: 0 667 1210 1875 Size: 667 543 665 Fragments arranged by size: 667 665 543 SmlI C'TyrA_G Cuts at: 0 429 1816 1875 Size: 429 1387 59 Fragments arranged by size: 1387 429 59 SrfI GCCC'GGGC Cuts at: 0 667 1875 Size: 667 1208 StyI C'CwwG_G Cuts at: 0 611 971 1541 1875 Size: 611 360 570 334 Fragments arranged by size: 611 570 360 334 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 812 1488 1875 Size: 812 676 387 Fragments arranged by size: 812 676 387 TatI w'GTAC_w Cuts at: 0 529 1875 Size: 529 1346 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1053 1875 Size: 1053 822 XmaI C'CCGG_G Cuts at: 0 665 1208 1875 Size: 665 543 667 Fragments arranged by size: 667 665 543 Enzymes that do cut: AleI AlwNI ApoI AvaI BanI BanII BbsI BbvCI BclI BglII BlpI BmgBI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BstXI BstYI BtgI BtsI DraI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I MmeI MscI MslI MspA1I PasI PflMI PfoI PpuMI PstI PvuII SacI SfcI SmaI SmlI SrfI StyI TaqII TatI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BfrBI BglI Bme1580I BmrI BmtI BplI BsaI BsaAI BsaBI BsaWI BsiWI BsmI BspEI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EagI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SspI StuI SwaI TstI Tth111I XbaI XhoI XmnI ZraI