(Circular) (Six-base) MAPSORT of: pFN21AE2607.seq Check: 7611 from: 1 to: 8017 pFN21AE2607 8017 bp With 182 enzymes: SgfI * September 11, 2017 14:08 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5827 278 Size: 53 83 186 936 4291 2468 Fragments arranged by size: 4291 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4155 5360 1937 Size: 2218 1205 4594 Fragments arranged by size: 4594 2218 1205 Acc65I G'GTAC_C Cuts at: 4766 5338 4766 Size: 572 7445 AclI AA'CG_TT Cuts at: 1563 6144 6517 1563 Size: 4581 373 3063 Fragments arranged by size: 4581 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2482 2495 3235 3314 3490 3698 3818 6080 Size: 850 13 740 79 176 208 120 2262 Cuts at: 6080 7464 7732 1632 Size: 1384 268 1917 Fragments arranged by size: 2262 1917 1384 850 740 268 208 176 120 79 13 AfeI AGC'GCT Cuts at: 4688 4688 Size: 8017 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7820 AflIII A'CryG_T Cuts at: 1203 3660 4893 6916 7893 1203 Size: 2457 1233 2023 977 1327 Fragments arranged by size: 2457 2023 1327 1233 977 AgeI A'CCGG_T Cuts at: 6848 6848 Size: 8017 AhdI GACnn_n'nnGTC Cuts at: 6746 6746 Size: 8017 AleI CACnn'nnGTG Cuts at: 1166 3515 1166 Size: 2349 5668 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8017 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4690 4722 4690 Size: 32 7985 AlwNI CAG_nnn'CTG Cuts at: 3762 3934 5760 7332 3762 Size: 172 1826 1572 4447 Fragments arranged by size: 4447 1826 1572 172 ApaLI G'TGCA_C Cuts at: 6074 7230 6074 Size: 1156 6861 ApoI r'AATT_y Cuts at: 1493 2131 2146 2294 2973 3153 3544 4030 5326 Size: 638 15 148 679 180 391 486 1296 Cuts at: 5326 5552 5704 7813 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1296 679 638 486 391 226 180 152 148 15 AseI AT'TA_AT Cuts at: 160 1032 6571 160 Size: 872 5539 1606 Fragments arranged by size: 5539 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8017 AvaI C'yCGr_G Cuts at: 1870 1943 2998 3873 4065 4304 4859 5342 1870 Size: 73 1055 875 192 239 555 483 4545 Fragments arranged by size: 4545 1055 875 555 483 239 192 73 BamHI G'GATC_C Cuts at: 4665 5347 5714 4665 Size: 682 367 6968 Fragments arranged by size: 6968 682 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2243 2415 2456 2552 3678 Size: 325 217 627 456 172 41 96 1126 Cuts at: 3678 4291 4747 4766 4955 5338 6793 618 Size: 613 456 19 189 383 1455 1842 Fragments arranged by size: 1842 1455 1126 627 613 456 456 383 325 217 189 172 96 41 19 BanII G_rGCy'C Cuts at: 1391 4725 5336 1391 Size: 3334 611 4072 Fragments arranged by size: 4072 3334 611 BbeI G_GCGC'C Cuts at: 2460 2460 Size: 8017 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2161 3624 3806 3921 4207 4225 5047 Size: 971 262 1463 182 115 286 18 822 Cuts at: 5047 5115 5154 5279 928 Size: 68 39 125 3666 Fragments arranged by size: 3666 1463 971 822 286 262 182 125 115 68 39 18 BbvCI CC'TCA_GC Cuts at: 2700 2700 Size: 8017 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3812 3846 6208 6242 1903 Size: 34 1875 34 2362 34 3678 Fragments arranged by size: 3678 2362 1875 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5254 5909 7119 5254 Size: 655 1210 6152 Fragments arranged by size: 6152 1210 655 BclI T'GATC_A Cuts at: 1548 1548 Size: 8017 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6628 136 Size: 107 122 71 631 605 4956 1525 Fragments arranged by size: 4956 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2493 8012 2493 Size: 5519 2498 BlpI GC'TnA_GC Cuts at: 2027 5434 2027 Size: 3407 4610 Bme1580I G_kGCm'C Cuts at: 1790 4752 4958 6078 7234 1790 Size: 2962 206 1120 1156 2573 Fragments arranged by size: 2962 2573 1156 1120 206 BmgBI CAC'GTC Cuts at: 1318 3661 4759 1318 Size: 2343 1098 4576 Fragments arranged by size: 4576 2343 1098 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2345 2713 4746 Size: 416 53 83 399 24 928 368 2033 Cuts at: 4746 4809 5748 6706 442 Size: 63 939 958 1753 Fragments arranged by size: 2033 1753 958 939 928 416 399 368 83 63 53 24 BmtI G_CTAG'C Cuts at: 1056 3027 1056 Size: 1971 6046 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2065 3326 3458 3995 4711 6677 1239 Size: 826 1261 132 537 716 1966 2579 Fragments arranged by size: 2579 1966 1261 826 716 537 132 Bpu10I CC'TnA_GC Cuts at: 2700 2700 Size: 8017 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5264 6142 7007 7305 5264 Size: 878 865 298 5976 Fragments arranged by size: 5976 878 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2668 3863 6680 882 Size: 617 1169 1195 2817 2219 Fragments arranged by size: 2817 2219 1195 1169 617 BsaAI yAC'GTr Cuts at: 493 1204 4267 4366 4773 5808 493 Size: 711 3063 99 407 1035 2702 Fragments arranged by size: 3063 2702 1035 711 407 99 BsaBI GATnn'nnATC Cuts at: 4595 4871 8011 4595 Size: 276 3140 4601 Fragments arranged by size: 4601 3140 276 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2307 2457 5824 6206 Size: 53 83 186 936 774 150 3367 382 Cuts at: 6206 6841 275 Size: 635 1451 Fragments arranged by size: 3367 1451 936 774 635 382 186 150 83 53 BsaWI w'CCGG_w Cuts at: 2835 3522 3609 6450 6848 7122 7269 7680 2835 Size: 687 87 2841 398 274 147 411 3172 Fragments arranged by size: 3172 2841 687 411 398 274 147 87 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2189 2219 1178 Size: 30 165 30 786 30 6976 Fragments arranged by size: 6976 786 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2636 3131 3134 3554 3863 1186 Size: 1450 495 3 420 309 5340 Fragments arranged by size: 5340 1450 495 420 309 3 BseYI C'CCAG_C Cuts at: 2406 3200 3836 4014 4414 7220 7716 2406 Size: 794 636 178 400 2806 496 2707 Fragments arranged by size: 2806 2707 794 636 496 400 178 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3748 4774 5030 1163 Size: 573 2012 1026 256 4150 Fragments arranged by size: 4150 2012 1026 573 256 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5467 6228 7256 1540 Size: 275 186 3466 761 1028 2301 Fragments arranged by size: 3466 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2099 4842 6078 6163 7234 2099 Size: 2743 1236 85 1071 2882 Fragments arranged by size: 2882 2743 1236 1071 85 BsmI GAATG_Cn' Cuts at: 5528 5621 5528 Size: 93 7924 BsmBI CGTCTCn'nnnn_ Cuts at: 2094 2094 Size: 8017 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2099 4725 4752 4842 4958 5336 6078 Size: 399 309 2626 27 90 116 378 742 Cuts at: 6078 6163 7234 1391 Size: 85 1071 2174 Fragments arranged by size: 2626 2174 1071 742 399 378 309 116 90 85 27 BspEI T'CCGG_A Cuts at: 2835 3609 7680 2835 Size: 774 4071 3172 Fragments arranged by size: 4071 3172 774 BspHI T'CATG_A Cuts at: 5178 5906 7636 5178 Size: 728 1730 5559 Fragments arranged by size: 5559 1730 728 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4854 4932 5372 844 Size: 4010 78 440 3489 Fragments arranged by size: 4010 3489 440 78 BsrBI CCG'CTC Cuts at: 1394 1823 5904 1394 Size: 429 4081 3507 Fragments arranged by size: 4081 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3226 4052 6512 6686 65 Size: 1183 1978 826 2460 174 1396 Fragments arranged by size: 2460 1978 1396 1183 826 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2502 6661 6848 1700 Size: 110 692 4159 187 2869 Fragments arranged by size: 4159 2869 692 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8017 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8017 BssSI C'ACGA_G Cuts at: 2789 6077 7089 2789 Size: 3288 1012 3717 Fragments arranged by size: 3717 3288 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3685 4591 4846 794 Size: 2891 906 255 3965 Fragments arranged by size: 3965 2891 906 255 BstBI TT'CG_AA Cuts at: 6821 6821 Size: 8017 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8017 BstXI CCAn_nnnn'nTGG Cuts at: 2927 4759 4879 2927 Size: 1832 120 6065 Fragments arranged by size: 6065 1832 120 BstYI r'GATC_y Cuts at: 1972 2493 2746 2898 4665 5347 5714 6099 6116 Size: 521 253 152 1767 682 367 385 17 Cuts at: 6116 7568 7654 7666 8012 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1767 1452 682 521 385 367 346 253 152 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3114 4670 3114 Size: 1556 6461 BtgI C'CryG_G Cuts at: 513 1065 2193 513 Size: 552 1128 6337 Fragments arranged by size: 6337 1128 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2500 2709 3924 3994 4225 487 Size: 683 56 1274 209 1215 70 231 4279 Fragments arranged by size: 4279 1274 1215 683 231 209 70 56 BtsI GCAGTG_nn' Cuts at: 4786 5533 6327 6347 4786 Size: 747 794 20 6456 Fragments arranged by size: 6456 794 747 20 ClaI AT'CG_AT Cuts at: 1554 4599 5245 1554 Size: 3045 646 4326 Fragments arranged by size: 4326 3045 646 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2872 2907 529 Size: 35 2308 35 5639 Fragments arranged by size: 5639 2308 35 35 DraI TTT'AAA Cuts at: 5321 5321 Size: 8017 DraIII CAC_nnn'GTG Cuts at: 3661 3897 4823 3661 Size: 236 926 6855 Fragments arranged by size: 6855 926 236 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7024 809 Size: 507 5708 1802 Fragments arranged by size: 5708 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2634 3628 3807 Size: 54 1425 50 275 18 804 994 179 Cuts at: 3807 4653 4731 4741 5464 6353 7982 8 Size: 846 78 10 723 889 1629 43 Fragments arranged by size: 1629 1425 994 889 846 804 723 275 179 78 54 50 43 18 10 EagI C'GGCC_G Cuts at: 1537 1812 5464 1537 Size: 275 3652 4090 Fragments arranged by size: 4090 3652 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3497 3595 3701 4343 4465 5947 1353 Size: 2144 98 106 642 122 1482 3423 Fragments arranged by size: 3423 2144 1482 642 122 106 98 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3416 6604 6978 7124 387 Size: 1064 138 1827 3188 374 146 1280 Fragments arranged by size: 3188 1827 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2065 2482 2495 3235 3314 3326 3458 Size: 393 433 417 13 740 79 12 132 Cuts at: 3458 3490 3698 3818 3995 4711 6080 6677 7464 Size: 32 208 120 177 716 1369 597 787 Cuts at: 7464 7732 1239 Size: 268 1524 Fragments arranged by size: 1524 1369 787 740 716 597 433 417 393 268 208 177 132 120 79 32 13 12 EcoNI CCTnn'n_nnAGG Cuts at: 3032 5009 3032 Size: 1977 6040 EcoO109I rG'GnC_Cy Cuts at: 4565 4565 Size: 8017 EcoRI G'AATT_C Cuts at: 3544 5326 5704 3544 Size: 1782 378 5857 Fragments arranged by size: 5857 1782 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4685 5104 4685 Size: 419 7598 FspI TGC'GCA Cuts at: 2489 6523 2489 Size: 4034 3983 HaeII r_GCGC'y Cuts at: 1678 2460 4690 7164 1678 Size: 782 2230 2474 2531 Fragments arranged by size: 2531 2474 2230 782 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2151 2183 6658 6690 6732 Size: 32 135 32 579 32 4475 32 42 Cuts at: 6732 6764 1373 Size: 32 2626 Fragments arranged by size: 4475 2626 579 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4156 4921 5361 5607 669 Size: 988 281 2218 765 440 246 3079 Fragments arranged by size: 3079 2218 988 765 440 281 246 HindIII A'AGCT_T Cuts at: 748 3296 3791 3951 5267 748 Size: 2548 495 160 1316 3498 Fragments arranged by size: 3498 2548 1316 495 160 HpaI GTT'AAC Cuts at: 5607 5607 Size: 8017 KasI G'GCGC_C Cuts at: 2456 2456 Size: 8017 KpnI G_GTAC'C Cuts at: 4770 5342 4770 Size: 572 7445 MfeI C'AATT_G Cuts at: 5616 5616 Size: 8017 MluI A'CGCG_T Cuts at: 7893 7893 Size: 8017 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2691 2907 3113 3522 7131 7315 1305 Size: 1386 216 206 409 3609 184 2007 Fragments arranged by size: 3609 2007 1386 409 216 206 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2636 3809 4655 4733 10 Size: 54 1425 343 804 1173 846 78 3294 Fragments arranged by size: 3294 1425 1173 846 804 343 78 54 MslI CAynn'nnrTG Cuts at: 518 1166 2444 3515 4892 5075 5975 6334 6493 Size: 648 1278 1071 1377 183 900 359 159 Cuts at: 6493 518 Size: 2042 Fragments arranged by size: 2042 1377 1278 1071 900 648 359 183 159 MspA1I CmG'CkG Cuts at: 1606 3292 3319 3931 5433 6110 7258 7503 1606 Size: 1686 27 612 1502 677 1148 245 2120 Fragments arranged by size: 2120 1686 1502 1148 677 612 245 27 NaeI GCC'GGC Cuts at: 2504 2504 Size: 8017 NarI GG'CG_CC Cuts at: 2457 2457 Size: 8017 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7465 NdeI CA'TA_TG Cuts at: 387 387 Size: 8017 NgoMIV G'CCGG_C Cuts at: 2502 2502 Size: 8017 NheI G'CTAG_C Cuts at: 1052 3023 1052 Size: 1971 6046 NotI GC'GGCC_GC Cuts at: 5464 5464 Size: 8017 NspI r_CATG'y Cuts at: 1132 1229 2488 4897 5375 6920 1132 Size: 97 1259 2409 478 1545 2229 Fragments arranged by size: 2409 2229 1545 1259 478 97 PasI CC'CwG_GG Cuts at: 1348 3778 1348 Size: 2430 5587 PciI A'CATG_T Cuts at: 4893 6916 4893 Size: 2023 5994 PflMI CCAn_nnn'nTGG Cuts at: 1108 2236 4080 1108 Size: 1128 1844 5045 Fragments arranged by size: 5045 1844 1128 PfoI T'CCnGG_A Cuts at: 2760 4220 4962 2760 Size: 1460 742 5815 Fragments arranged by size: 5815 1460 742 PmeI GTTT'AAAC Cuts at: 5321 5321 Size: 8017 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6086 6118 7599 7631 6086 Size: 32 1481 32 6472 Fragments arranged by size: 6472 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4565 4565 Size: 8017 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8017 PsiI TTA'TAA Cuts at: 4577 5587 4577 Size: 1010 7007 PstI C_TGCA'G Cuts at: 830 3534 4587 5024 5369 830 Size: 2704 1053 437 345 3478 Fragments arranged by size: 3478 2704 1053 437 345 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8017 PvuII CAG'CTG Cuts at: 3292 3319 3931 3292 Size: 27 612 7378 Fragments arranged by size: 7378 612 27 SalI G'TCGA_C Cuts at: 1936 4154 5359 1936 Size: 2218 1205 4594 Fragments arranged by size: 4594 2218 1205 SbfI CC_TGCA'GG Cuts at: 5369 5369 Size: 8017 ScaI AGT'ACT Cuts at: 5018 6265 7977 5018 Size: 1247 1712 5058 Fragments arranged by size: 5058 1712 1247 SexAI A'CCwGG_T Cuts at: 2433 4977 2433 Size: 2544 5473 SfcI C'TryA_G Cuts at: 826 1045 3530 4583 5020 5365 6500 7181 7372 Size: 219 2485 1053 437 345 1135 681 191 Cuts at: 7372 826 Size: 1471 Fragments arranged by size: 2485 1471 1135 1053 681 437 345 219 191 SfoI GGC'GCC Cuts at: 2458 2458 Size: 8017 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8017 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8017 SmaI CCC'GGG Cuts at: 1872 3875 5344 1872 Size: 2003 1469 4545 Fragments arranged by size: 4545 2003 1469 SmlI C'TyrA_G Cuts at: 820 1017 1943 5279 6121 7022 7284 820 Size: 197 926 3336 842 901 262 1553 Fragments arranged by size: 3336 1553 926 901 842 262 197 SnaBI TAC'GTA Cuts at: 493 4366 5808 493 Size: 3873 1442 2702 Fragments arranged by size: 3873 2702 1442 SpeI A'CTAG_T Cuts at: 152 152 Size: 8017 SphI G_CATG'C Cuts at: 1132 2488 5375 1132 Size: 1356 2887 3774 Fragments arranged by size: 3774 2887 1356 SspI AAT'ATT Cuts at: 5 52 2629 5941 5 Size: 47 2577 3312 2081 Fragments arranged by size: 3312 2577 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8017 StyI C'CwwG_G Cuts at: 513 1065 2187 2289 2920 4113 5456 513 Size: 552 1122 102 631 1193 1343 3074 Fragments arranged by size: 3074 1343 1193 1122 631 552 102 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2261 2443 2570 3485 4699 6044 6061 Size: 1255 681 182 127 915 1214 1345 17 Cuts at: 6061 6214 6399 6844 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1345 1255 1214 915 681 445 185 182 153 127 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2076 2574 4317 Size: 274 80 33 51 1443 99 498 1743 Cuts at: 4317 5016 6263 7975 96 Size: 699 1247 1712 138 Fragments arranged by size: 1743 1712 1443 1247 699 498 274 138 99 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2189 2221 2731 2742 2763 2774 7977 8009 2189 Size: 32 510 11 21 11 5203 32 2197 Fragments arranged by size: 5203 2197 510 32 32 21 11 11 Tth111I GACn'n_nGTC Cuts at: 1316 4979 1316 Size: 3663 4354 XbaI T'CTAG_A Cuts at: 5353 5353 Size: 8017 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4912 4912 Size: 8017 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8017 XmaI C'CCGG_G Cuts at: 1870 3873 5342 1870 Size: 2003 1469 4545 Fragments arranged by size: 4545 2003 1469 XmnI GAAnn'nnTTC Cuts at: 5087 6146 5087 Size: 1059 6958 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5825 276 Size: 53 83 186 936 4291 2468 Fragments arranged by size: 4291 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI