Restriction map for
pFN21AE2186
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AflIII AgeI AhdI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BamHI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BsaWI BseAI
BseRI BsiWI BsmBI BspDI BspEI
BspLU11I BsrDI BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DraIII Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI MaeI MfeI
MluI MroI MseI MunI NdeI
NheI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI SbfI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI TfiI
Tru9I Tsp509I Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 657 GGTACC G^GTACC
AccI 964 GTCTAC GT^[AC][GT]AC
Asp718I 657 GGTACC G^GTACC
BbrPI 1750 CACGTG CAC^GTG
BbsI 952 GTCTTC GAAGAC(2/6)
BlpI 1131 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1131 GCTGAGC GC^T[ACGT]AGC
BpuAI 952 GTCTTC GAAGAC(2/6)
BsaBI 1341 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 922 GAGACC GGTCTC(1/5)
BsgI 935 CTGCAC GTGCAG(16/14)
BsmAI 922 GAGAC GTCTC(1/5)
BsmI 541 GAATGC GAATGC(1/-1)
Bsp1407I 266 TGTACA T^GTACA
BspHI 1337 TCATGA T^CATGA
BsrGI 266 TGTACA T^GTACA
BstAPI 389 GCATGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1131 GCTGAGC GC^T[ACGT]AGC
EagI 71 CGGCCG C^GGCCG
EclXI 71 CGGCCG C^GGCCG
Eco52I 71 CGGCCG C^GGCCG
KpnI 657 GGTACC GGTAC^C
MaeIII 606 GTCAC ^GT[ACGT]AC
MamI 1341 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1649 CCATGG C^CATGG
NotI 70 GCGGCCGC GC^GGCCGC
NruI 576 TCGCGA TCG^CGA
NspI 149 ACATGC [GA]CATG^[CT]
PmaCI 1750 CACGTG CAC^GTG
PmlI 1750 CACGTG CAC^GTG
PstI 891 CTGCAG CTGCA^G
PvuII 55 CAGCTG CAG^CTG
RcaI 1337 TCATGA T^CATGA
SacI 1134 GAGCTC GAGCT^C
SexAI 1015 ACCTGGT A^CC[AT]GGT
SfcI 891 CTGCAG C^T[GA][CT]AG
SmaI 1458 CCCGGG CCC^GGG
SmlI 1513 CTCAAG C^T[CT][GA]AG
SspBI 266 TGTACA T^GTACA
TaqI 776 TCGA T^CGA
Tsp45I 606 GTCAC ^GT[GC]AC
TthHB8I 776 TCGA T^CGA
XmaCI 1458 CCCGGG C^CCGGG
XmaI 1458 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Alw44I 199 GTGCAC G^TGCAC
942 GTGCAC
AlwNI 1183 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
1602 CAGCCGCTG
ApaLI 199 GTGCAC G^TGCAC
942 GTGCAC
BalI 761 TGGCCA TGG^CCA
980 TGGCCA
BpmI 193 CTGGAG CTGGAG(16/14)
1327 CTGGAG
BsaAI 1171 TACGTG [CT]AC^GT[GA]
1750 CACGTG
BsiEI 71 CGGCCG CG[GA][CT]^CG
399 CGACCG
BssHII 330 GCGCGC G^CGCGC
1482 GCGCGC
BstXI 752 CCACCACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1382 CCAGCATTGTGG
BstYI 224 AGATCC [GA]^GATC[CT]
1559 GGATCT
DdeI 800 CTGAG C^T[ACGT]AG
1132 CTGAG
DrdI 424 GACGCCGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1575 GACGGTGGAGTC
EarI 1249 CTCTTC CTCTTC(1/4)
1276 CTCTTC
HinfI 604 GAGTC G^A[ACGT]TC
1582 GAGTC
Ksp632I 1249 CTCTTC CTCTTC(1/4)
1276 CTCTTC
KspI 123 CCGCGG CCGC^GG
1621 CCGCGG
MflI 224 AGATCC [GA]^GATC[CT]
1559 GGATCT
MluNI 761 TGGCCA TGG^CCA
980 TGGCCA
MscI 761 TGGCCA TGG^CCA
980 TGGCCA
PflMI 704 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
989 CCATCTGGTGG
PleI 604 GAGTC GAGTC(4/5)
1582 GAGTC
PpuMI 431 AGGTCCT [GA]G^G[AT]CC[CT]
1148 GGGACCC
SacII 123 CCGCGG CCGC^GG
1621 CCGCGG
SapI 1248 GCTCTTC GCTCTTC(1/4)
1275 GCTCTTC
StuI 481 AGGCCT AGG^CCT
717 AGGCCT
Van91I 704 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
989 CCATCTGGTGG
XcmI 1115 CCATCACGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1382 CCAGCATTGTGGTGG
XhoII 224 AGATCC [GA]^GATC[CT]
1559 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BglI BmyI BsaHI BsaJI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI KasI MaeII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfoI StyI TseI TspRI