(Circular) (Six-base) MAPSORT of: pFN21AE2186.seq Check: 639 from: 1 to: 6460 pFN21AE2186 6460 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3461 3730 3461 Size: 269 6191 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4270 278 Size: 53 83 186 936 2734 2468 Fragments arranged by size: 2734 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2970 3803 1937 Size: 1033 833 4594 Fragments arranged by size: 4594 1033 833 Acc65I G'GTAC_C Cuts at: 2662 3781 2662 Size: 1119 5341 AclI AA'CG_TT Cuts at: 1563 4587 4960 1563 Size: 3024 373 3063 Fragments arranged by size: 3063 3024 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2624 2668 4523 5907 6175 1632 Size: 992 44 1855 1384 268 1917 Fragments arranged by size: 1917 1855 1384 992 268 44 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6263 AflIII A'CryG_T Cuts at: 1203 5359 6336 1203 Size: 4156 977 1327 Fragments arranged by size: 4156 1327 977 AgeI A'CCGG_T Cuts at: 5291 5291 Size: 6460 AhdI GACnn_n'nnGTC Cuts at: 5189 5189 Size: 6460 AleI CACnn'nnGTG Cuts at: 1166 2762 1166 Size: 1596 4864 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6460 AlwNI CAG_nnn'CTG Cuts at: 3193 3612 4203 5775 3193 Size: 419 591 1572 3878 Fragments arranged by size: 3878 1572 591 419 ApaI G_GGCC'C Cuts at: 2564 2939 3224 3719 2564 Size: 375 285 495 5305 Fragments arranged by size: 5305 495 375 285 ApaLI G'TGCA_C Cuts at: 2204 2947 4517 5673 2204 Size: 743 1570 1156 2991 Fragments arranged by size: 2991 1570 1156 743 ApoI r'AATT_y Cuts at: 1493 3769 3995 4147 6256 1493 Size: 2276 226 152 2109 1697 Fragments arranged by size: 2276 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5014 160 Size: 872 3982 1606 Fragments arranged by size: 3982 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6460 AvaI C'yCGr_G Cuts at: 1870 1943 2387 2536 3463 3497 3674 3785 1870 Size: 73 444 149 927 34 177 111 4545 Fragments arranged by size: 4545 927 444 177 149 111 73 34 BamHI G'GATC_C Cuts at: 3790 4157 3790 Size: 367 6093 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2116 2476 2527 2647 2662 Size: 325 217 627 329 360 51 120 15 Cuts at: 2662 3314 3650 3781 5236 618 Size: 652 336 131 1455 1842 Fragments arranged by size: 1842 1455 652 627 360 336 329 325 217 131 120 51 15 BanII G_rGCy'C Cuts at: 1391 2564 2589 2939 3143 3224 3719 3779 1391 Size: 1173 25 350 204 81 495 60 4072 Fragments arranged by size: 4072 1173 495 350 204 81 60 25 BbeI G_GCGC'C Cuts at: 2480 2531 3654 2480 Size: 51 1123 5286 Fragments arranged by size: 5286 1123 51 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2950 928 Size: 971 1051 4438 Fragments arranged by size: 4438 1051 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4651 4685 1903 Size: 34 2714 34 3678 Fragments arranged by size: 3678 2714 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4352 5562 4352 Size: 1210 5250 BclI T'GATC_A Cuts at: 1548 1548 Size: 6460 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2373 2474 2485 Size: 107 122 71 631 605 701 101 11 Cuts at: 2485 2891 5071 136 Size: 406 2180 1525 Fragments arranged by size: 2180 1525 701 631 605 406 122 107 101 71 11 BglII A'GATC_T Cuts at: 6455 6455 Size: 6460 BlpI GC'TnA_GC Cuts at: 3137 3877 3137 Size: 740 5720 Bme1580I G_kGCm'C Cuts at: 1790 2121 2208 2564 2598 2652 2939 2951 3224 Size: 331 87 356 34 54 287 12 273 Cuts at: 3224 3251 3719 4521 5677 1790 Size: 27 468 802 1156 2573 Fragments arranged by size: 2573 1156 802 468 356 331 287 273 87 54 34 27 12 BmgBI CAC'GTC Cuts at: 1318 2754 3552 1318 Size: 1436 798 4226 Fragments arranged by size: 4226 1436 798 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3152 3514 4191 Size: 416 53 83 399 24 1735 362 677 Cuts at: 4191 5149 442 Size: 958 1753 Fragments arranged by size: 1753 1735 958 677 416 399 362 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6460 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2219 3353 5120 1239 Size: 980 1134 1767 2579 Fragments arranged by size: 2579 1767 1134 980 Bpu10I CC'TnA_GC Cuts at: 2805 2805 Size: 6460 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3503 4585 5450 5748 3503 Size: 1082 865 298 4215 Fragments arranged by size: 4215 1082 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2921 5123 882 Size: 617 1422 2202 2219 Fragments arranged by size: 2219 2202 1422 617 BsaAI yAC'GTr Cuts at: 493 1204 3178 3757 4251 493 Size: 711 1974 579 494 2702 Fragments arranged by size: 2702 1974 711 579 494 BsaBI GATnn'nnATC Cuts at: 3350 6454 3350 Size: 3104 3356 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2430 2477 2528 3558 Size: 53 83 186 936 897 47 51 1030 Cuts at: 3558 3651 4267 4649 5284 275 Size: 93 616 382 635 1451 Fragments arranged by size: 1451 1030 936 897 635 616 382 186 93 83 53 51 47 BsaWI w'CCGG_w Cuts at: 4893 5291 5565 5712 6123 4893 Size: 398 274 147 411 5230 Fragments arranged by size: 5230 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6235 Fragments arranged by size: 6235 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6460 BseYI C'CCAG_C Cuts at: 2419 2494 2555 2851 3107 3217 3435 5663 6159 Size: 75 61 296 256 110 218 2228 496 Cuts at: 6159 2419 Size: 2720 Fragments arranged by size: 2720 2228 496 296 256 218 110 75 61 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2925 1163 Size: 573 1189 4698 Fragments arranged by size: 4698 1189 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2079 2407 3910 4671 5699 1540 Size: 275 186 78 328 1503 761 1028 2301 Fragments arranged by size: 2301 1503 1028 761 328 275 186 78 BsiHKAI G_wGCw'C Cuts at: 2208 2910 2951 3143 3234 3513 4521 4606 5677 Size: 702 41 192 91 279 1008 85 1071 Cuts at: 5677 2208 Size: 2991 Fragments arranged by size: 2991 1071 1008 702 279 192 91 85 41 BsmI GAATG_Cn' Cuts at: 2552 3971 4064 2552 Size: 1419 93 4948 Fragments arranged by size: 4948 1419 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2121 2208 2564 2589 2598 2652 2910 Size: 399 331 87 356 25 9 54 258 Cuts at: 2910 2939 2951 3143 3224 3234 3251 3513 3719 Size: 29 12 192 81 10 17 262 206 Cuts at: 3719 3779 4521 4606 5677 1391 Size: 60 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 356 331 262 258 206 192 87 85 81 60 54 29 25 17 12 10 9 BspEI T'CCGG_A Cuts at: 6123 6123 Size: 6460 BspHI T'CATG_A Cuts at: 3342 4349 6079 3342 Size: 1007 1730 3723 Fragments arranged by size: 3723 1730 1007 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2256 3246 3461 3730 3815 844 Size: 1412 990 215 269 85 3489 Fragments arranged by size: 3489 1412 990 269 215 85 BsrBI CCG'CTC Cuts at: 1394 1823 2322 2620 3645 4347 1394 Size: 429 499 298 1025 702 3507 Fragments arranged by size: 3507 1025 702 499 429 298 BsrDI GCAATG_nn' Cuts at: 65 1248 4955 5129 65 Size: 1183 3707 174 1396 Fragments arranged by size: 3707 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2345 2530 3035 3164 3476 5104 5291 Size: 110 535 185 505 129 312 1628 187 Cuts at: 5291 1700 Size: 2869 Fragments arranged by size: 2869 1628 535 505 312 187 185 129 110 BsrGI T'GTAC_A Cuts at: 96 2271 96 Size: 2175 4285 BssHII G'CGCG_C Cuts at: 1925 2335 3487 1925 Size: 410 1152 4898 Fragments arranged by size: 4898 1152 410 BssSI C'ACGA_G Cuts at: 4520 5532 4520 Size: 1012 5448 BstAPI GCAn_nnn'nTGC Cuts at: 794 2400 794 Size: 1606 4854 BstBI TT'CG_AA Cuts at: 5264 5264 Size: 6460 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6460 BstXI CCAn_nnnn'nTGG Cuts at: 2764 3394 2764 Size: 630 5830 BstYI r'GATC_y Cuts at: 1972 2229 3564 3790 4157 4542 4559 6011 6097 Size: 257 1335 226 367 385 17 1452 86 Cuts at: 6097 6109 6455 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1335 385 367 346 257 226 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2128 2472 2931 3144 3626 3654 Size: 552 939 124 344 459 213 482 28 Cuts at: 3654 513 Size: 3319 Fragments arranged by size: 3319 939 552 482 459 344 213 124 28 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3046 487 Size: 683 56 1820 3901 Fragments arranged by size: 3901 1820 683 56 BtsI GCAGTG_nn' Cuts at: 3976 4770 4790 3976 Size: 794 20 5646 Fragments arranged by size: 5646 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6460 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6425 DraI TTT'AAA Cuts at: 3764 3764 Size: 6460 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2435 3586 5467 809 Size: 507 1119 1151 1881 1802 Fragments arranged by size: 1881 1802 1151 1119 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2076 2216 2766 Size: 54 1425 50 275 18 246 140 550 Cuts at: 2766 2985 3030 3162 3478 3634 3657 3907 4796 Size: 219 45 132 316 156 23 250 889 Cuts at: 4796 6425 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 550 316 275 250 246 219 156 140 132 54 50 45 43 23 18 EagI C'GGCC_G Cuts at: 1537 1812 2076 3907 1537 Size: 275 264 1831 4090 Fragments arranged by size: 4090 1831 275 264 EarI CTCTTCn'nnn_ Cuts at: 1353 3260 3287 4390 1353 Size: 1907 27 1103 3423 Fragments arranged by size: 3423 1907 1103 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5047 5421 5567 387 Size: 1064 138 3458 374 146 1280 Fragments arranged by size: 3458 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3141 3141 Size: 6460 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2219 2624 2668 3353 4523 5120 5907 Size: 393 587 405 44 685 1170 597 787 Cuts at: 5907 6175 1239 Size: 268 1524 Fragments arranged by size: 1524 1170 787 685 597 587 405 393 268 44 EcoO109I rG'GnC_Cy Cuts at: 2092 2437 2560 2936 3154 3716 2092 Size: 345 123 376 218 562 4836 Fragments arranged by size: 4836 562 376 345 218 123 EcoRI G'AATT_C Cuts at: 3769 4147 3769 Size: 378 6082 FspI TGC'GCA Cuts at: 4966 4966 Size: 6460 HaeII r_GCGC'y Cuts at: 1678 2480 2531 2812 3137 3449 3654 3687 5607 Size: 802 51 281 325 312 205 33 1920 Cuts at: 5607 1678 Size: 2531 Fragments arranged by size: 2531 1920 802 325 312 281 205 51 33 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5101 5133 5175 5207 1373 Size: 32 135 32 3529 32 42 32 2626 Fragments arranged by size: 3529 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3804 4050 669 Size: 988 281 1866 246 3079 Fragments arranged by size: 3079 1866 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6460 HpaI GTT'AAC Cuts at: 4050 4050 Size: 6460 KasI G'GCGC_C Cuts at: 2476 2527 3650 2476 Size: 51 1123 5286 Fragments arranged by size: 5286 1123 51 KpnI G_GTAC'C Cuts at: 2666 3785 2666 Size: 1119 5341 MfeI C'AATT_G Cuts at: 4059 4059 Size: 6460 MluI A'CGCG_T Cuts at: 6336 6336 Size: 6460 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5574 5758 1305 Size: 4269 184 2007 Fragments arranged by size: 4269 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2768 2987 10 Size: 54 1425 343 936 219 3483 Fragments arranged by size: 3483 1425 936 343 219 54 MslI CAynn'nnrTG Cuts at: 518 1166 2762 2783 3395 4418 4777 4936 518 Size: 648 1596 21 612 1023 359 159 2042 Fragments arranged by size: 2042 1596 1023 648 612 359 159 21 MspA1I CmG'CkG Cuts at: 1606 2062 2130 2221 2308 2359 2555 3226 3612 Size: 456 68 91 87 51 196 671 386 Cuts at: 3612 3628 3876 4553 5701 5946 1606 Size: 16 248 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 671 456 386 248 245 196 91 87 68 51 16 NaeI GCC'GGC Cuts at: 2347 2532 3037 3166 2347 Size: 185 505 129 5641 Fragments arranged by size: 5641 505 185 129 NarI GG'CG_CC Cuts at: 2477 2528 3651 2477 Size: 51 1123 5286 Fragments arranged by size: 5286 1123 51 NcoI C'CATG_G Cuts at: 513 1065 2004 3654 513 Size: 552 939 1650 3319 Fragments arranged by size: 3319 1650 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6460 NgoMIV G'CCGG_C Cuts at: 2345 2530 3035 3164 2345 Size: 185 505 129 5641 Fragments arranged by size: 5641 505 185 129 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6460 NotI GC'GGCC_GC Cuts at: 2076 3907 2076 Size: 1831 4629 NruI TCG'CGA Cuts at: 2583 2583 Size: 6460 NspI r_CATG'y Cuts at: 1132 1229 2158 3818 5363 1132 Size: 97 929 1660 1545 2229 Fragments arranged by size: 2229 1660 1545 929 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6460 PciI A'CATG_T Cuts at: 5359 5359 Size: 6460 PflMI CCAn_nnn'nTGG Cuts at: 1108 2715 3000 1108 Size: 1607 285 4568 Fragments arranged by size: 4568 1607 285 PmeI GTTT'AAAC Cuts at: 3764 3764 Size: 6460 PmlI CAC'GTG Cuts at: 3757 3757 Size: 6460 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2687 2719 3182 3214 4529 4561 6042 6074 2687 Size: 32 463 32 1315 32 1481 32 3073 Fragments arranged by size: 3073 1481 1315 463 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2437 3154 2437 Size: 717 5743 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6460 PsiI TTA'TAA Cuts at: 4030 4030 Size: 6460 PspOMI G'GGCC_C Cuts at: 2560 2935 3220 3715 2560 Size: 375 285 495 5305 Fragments arranged by size: 5305 495 375 285 PstI C_TGCA'G Cuts at: 830 2900 3812 830 Size: 2070 912 3478 Fragments arranged by size: 3478 2070 912 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6460 PvuII CAG'CTG Cuts at: 2062 2062 Size: 6460 SacI G_AGCT'C Cuts at: 3143 3143 Size: 6460 SacII CC_GC'GG Cuts at: 2131 3629 2131 Size: 1498 4962 SalI G'TCGA_C Cuts at: 1936 3802 1936 Size: 1866 4594 SanDI GG'GwC_CC Cuts at: 3154 3154 Size: 6460 SapI GCTCTTCn'nnn_ Cuts at: 3260 3287 3260 Size: 27 6433 SbfI CC_TGCA'GG Cuts at: 3812 3812 Size: 6460 ScaI AGT'ACT Cuts at: 3080 3503 3524 4708 6420 3080 Size: 423 21 1184 1712 3120 Fragments arranged by size: 3120 1712 1184 423 21 SexAI A'CCwGG_T Cuts at: 3020 3020 Size: 6460 SfcI C'TryA_G Cuts at: 826 1045 2896 3808 4943 5624 5815 826 Size: 219 1851 912 1135 681 191 1471 Fragments arranged by size: 1851 1471 1135 912 681 219 191 SfoI GGC'GCC Cuts at: 2478 2529 3652 2478 Size: 51 1123 5286 Fragments arranged by size: 5286 1123 51 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6460 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6460 SmaI CCC'GGG Cuts at: 1872 3465 3787 1872 Size: 1593 322 4545 Fragments arranged by size: 4545 1593 322 SmlI C'TyrA_G Cuts at: 820 1017 1943 3518 4564 5465 5727 820 Size: 197 926 1575 1046 901 262 1553 Fragments arranged by size: 1575 1553 1046 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4251 493 Size: 3758 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6460 SphI G_CATG'C Cuts at: 1132 3818 1132 Size: 2686 3774 SrfI GCCC'GGGC Cuts at: 3465 3465 Size: 6460 SspI AAT'ATT Cuts at: 5 52 4384 5 Size: 47 4332 2081 Fragments arranged by size: 4332 2081 47 StuI AGG'CCT Cuts at: 1517 2488 2724 1517 Size: 971 236 5253 Fragments arranged by size: 5253 971 236 StyI C'CwwG_G Cuts at: 513 1065 2004 2088 2340 3654 3899 513 Size: 552 939 84 252 1314 245 3074 Fragments arranged by size: 3074 1314 939 552 252 245 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3496 4487 4504 4657 4842 5287 325 Size: 1255 1916 991 17 153 185 445 1498 Fragments arranged by size: 1916 1498 1255 991 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2271 3078 3501 Size: 274 80 33 51 1443 294 807 423 Cuts at: 3501 3522 4706 6418 96 Size: 21 1184 1712 138 Fragments arranged by size: 1712 1443 1184 807 423 294 274 138 80 51 33 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6420 6452 6420 Size: 32 6428 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6460 XbaI T'CTAG_A Cuts at: 3796 3796 Size: 6460 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3127 3394 3127 Size: 267 6193 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6460 XmaI C'CCGG_G Cuts at: 1870 3463 3785 1870 Size: 1593 322 4545 Fragments arranged by size: 4545 1593 322 XmnI GAAnn'nnTTC Cuts at: 4589 4589 Size: 6460 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4268 276 Size: 53 83 186 936 2734 2468 Fragments arranged by size: 2734 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NsiI PacI PfoI PspXI PsrI RsrII SfiI SwaI