Restriction map for
pFN21AE2186
(Linear) (Six-base) MAPSORT of: KIEE2186.seq Check: 140 from: 1 to: 1755
KIEE2186.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1456 1725 1755
Size: 1456 269 30
Fragments arranged by size:
1456 269 30
AccI GT'mk_AC
Cuts at: 0 965 1755
Size: 965 790
Acc65I G'GTAC_C
Cuts at: 0 657 1755
Size: 657 1098
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 663 1755
Size: 619 44 1092
Fragments arranged by size:
1092 619 44
AleI CACnn'nnGTG
Cuts at: 0 757 1755
Size: 757 998
AlwNI CAG_nnn'CTG
Cuts at: 0 1188 1607 1755
Size: 1188 419 148
Fragments arranged by size:
1188 419 148
ApaI G_GGCC'C
Cuts at: 0 559 934 1219 1714 1755
Size: 559 375 285 495 41
Fragments arranged by size:
559 495 375 285 41
ApaLI G'TGCA_C
Cuts at: 0 199 942 1755
Size: 199 743 813
Fragments arranged by size:
813 743 199
AvaI C'yCGr_G
Cuts at: 0 382 531 1458 1492 1669 1755
Size: 382 149 927 34 177 86
Fragments arranged by size:
927 382 177 149 86 34
BanI G'GyrC_C
Cuts at: 0 111 471 522 642 657 1309 1645 1755
Size: 111 360 51 120 15 652 336 110
Fragments arranged by size:
652 360 336 120 111 110 51 15
BanII G_rGCy'C
Cuts at: 0 559 584 934 1138 1219 1714 1755
Size: 559 25 350 204 81 495 41
Fragments arranged by size:
559 495 350 204 81 41 25
BbeI G_GCGC'C
Cuts at: 0 475 526 1649 1755
Size: 475 51 1123 106
Fragments arranged by size:
1123 475 106 51
BbsI GAAGACnn'nnnn_
Cuts at: 0 945 1755
Size: 945 810
BglI GCCn_nnn'nGGC
Cuts at: 0 368 469 480 886 1755
Size: 368 101 11 406 869
Fragments arranged by size:
869 406 368 101 11
BlpI GC'TnA_GC
Cuts at: 0 1132 1755
Size: 1132 623
Bme1580I G_kGCm'C
Cuts at: 0 116 203 559 593 647 934 946 1219
Size: 116 87 356 34 54 287 12 273
Cuts at: 1219 1246 1714 1755
Size: 27 468 41
Fragments arranged by size:
468 356 287 273 116 87 54 41
34 27 12
BmgBI CAC'GTC
Cuts at: 0 749 1547 1755
Size: 749 798 208
Fragments arranged by size:
798 749 208
BmrI ACTGGGnnnn_n'
Cuts at: 0 1147 1509 1755
Size: 1147 362 246
Fragments arranged by size:
1147 362 246
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 1348 1755
Size: 214 1134 407
Fragments arranged by size:
1134 407 214
Bpu10I CC'TnA_GC
Cuts at: 0 800 1755
Size: 800 955
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1498 1755
Size: 1498 257
BsaI GGTCTCn'nnnn_
Cuts at: 0 916 1755
Size: 916 839
BsaAI yAC'GTr
Cuts at: 0 1173 1752 1755
Size: 1173 579 3
Fragments arranged by size:
1173 579 3
BsaBI GATnn'nnATC
Cuts at: 0 1345 1755
Size: 1345 410
BsaHI Gr'CG_yC
Cuts at: 0 425 472 523 1553 1646 1755
Size: 425 47 51 1030 93 109
Fragments arranged by size:
1030 425 109 93 51 47
BseYI C'CCAG_C
Cuts at: 0 414 489 550 846 1102 1212 1430 1755
Size: 414 75 61 296 256 110 218 325
Fragments arranged by size:
414 325 296 256 218 110 75 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 920 1755
Size: 920 835
BsiEI CG_ry'CG
Cuts at: 0 74 402 1755
Size: 74 328 1353
Fragments arranged by size:
1353 328 74
BsiHKAI G_wGCw'C
Cuts at: 0 203 905 946 1138 1229 1508 1755
Size: 203 702 41 192 91 279 247
Fragments arranged by size:
702 279 247 203 192 91 41
BsmI GAATG_Cn'
Cuts at: 0 547 1755
Size: 547 1208
Bsp1286I G_dGCh'C
Cuts at: 0 116 203 559 584 593 647 905 934
Size: 116 87 356 25 9 54 258 29
Cuts at: 934 946 1138 1219 1229 1246 1508 1714 1755
Size: 12 192 81 10 17 262 206 41
Fragments arranged by size:
356 262 258 206 192 116 87 81
54 41 29 25 17 12 10 9
BspHI T'CATG_A
Cuts at: 0 1337 1755
Size: 1337 418
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 251 1241 1456 1725 1755
Size: 251 990 215 269 30
Fragments arranged by size:
990 269 251 215 30
BsrBI CCG'CTC
Cuts at: 0 317 615 1640 1755
Size: 317 298 1025 115
Fragments arranged by size:
1025 317 298 115
BsrFI r'CCGG_y
Cuts at: 0 340 525 1030 1159 1471 1755
Size: 340 185 505 129 312 284
Fragments arranged by size:
505 340 312 284 185 129
BsrGI T'GTAC_A
Cuts at: 0 266 1755
Size: 266 1489
BssHII G'CGCG_C
Cuts at: 0 330 1482 1755
Size: 330 1152 273
Fragments arranged by size:
1152 330 273
BstAPI GCAn_nnn'nTGC
Cuts at: 0 395 1755
Size: 395 1360
BstXI CCAn_nnnn'nTGG
Cuts at: 0 759 1389 1755
Size: 759 630 366
Fragments arranged by size:
759 630 366
BstYI r'GATC_y
Cuts at: 0 224 1559 1755
Size: 224 1335 196
Fragments arranged by size:
1335 224 196
BtgI C'CryG_G
Cuts at: 0 123 467 926 1139 1621 1649 1755
Size: 123 344 459 213 482 28 106
Fragments arranged by size:
482 459 344 213 123 106 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1041 1755
Size: 1041 714
DrdI GACnn_nn'nnGTC
Cuts at: 0 430 1581 1755
Size: 430 1151 174
Fragments arranged by size:
1151 430 174
EaeI y'GGCC_r
Cuts at: 0 71 211 761 980 1025 1157 1473 1629
Size: 71 140 550 219 45 132 316 156
Cuts at: 1629 1652 1755
Size: 23 103
Fragments arranged by size:
550 316 219 156 140 132 103 71
45 23
EagI C'GGCC_G
Cuts at: 0 71 1755
Size: 71 1684
EarI CTCTTCn'nnn_
Cuts at: 0 1255 1282 1755
Size: 1255 27 473
Fragments arranged by size:
1255 473 27
EcoICRI GAG'CTC
Cuts at: 0 1136 1755
Size: 1136 619
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 619 663 1348 1755
Size: 214 405 44 685 407
Fragments arranged by size:
685 407 405 214 44
EcoO109I rG'GnC_Cy
Cuts at: 0 87 432 555 931 1149 1711 1755
Size: 87 345 123 376 218 562 44
Fragments arranged by size:
562 376 345 218 123 87 44
HaeII r_GCGC'y
Cuts at: 0 475 526 807 1132 1444 1649 1682 1755
Size: 475 51 281 325 312 205 33 73
Fragments arranged by size:
475 325 312 281 205 73 51 33
KasI G'GCGC_C
Cuts at: 0 471 522 1645 1755
Size: 471 51 1123 110
Fragments arranged by size:
1123 471 110 51
KpnI G_GTAC'C
Cuts at: 0 661 1755
Size: 661 1094
MscI TGG'CCA
Cuts at: 0 763 982 1755
Size: 763 219 773
Fragments arranged by size:
773 763 219
MslI CAynn'nnrTG
Cuts at: 0 757 778 1390 1755
Size: 757 21 612 365
Fragments arranged by size:
757 612 365 21
MspA1I CmG'CkG
Cuts at: 0 57 125 216 303 354 550 1221 1607
Size: 57 68 91 87 51 196 671 386
Cuts at: 1607 1623 1755
Size: 16 132
Fragments arranged by size:
671 386 196 132 91 87 68 57
51 16
NaeI GCC'GGC
Cuts at: 0 342 527 1032 1161 1755
Size: 342 185 505 129 594
Fragments arranged by size:
594 505 342 185 129
NarI GG'CG_CC
Cuts at: 0 472 523 1646 1755
Size: 472 51 1123 109
Fragments arranged by size:
1123 472 109 51
NcoI C'CATG_G
Cuts at: 0 1649 1755
Size: 1649 106
NgoMIV G'CCGG_C
Cuts at: 0 340 525 1030 1159 1755
Size: 340 185 505 129 596
Fragments arranged by size:
596 505 340 185 129
NotI GC'GGCC_GC
Cuts at: 0 71 1755
Size: 71 1684
NruI TCG'CGA
Cuts at: 0 578 1755
Size: 578 1177
NspI r_CATG'y
Cuts at: 0 153 1755
Size: 153 1602
PflMI CCAn_nnn'nTGG
Cuts at: 0 710 995 1755
Size: 710 285 760
Fragments arranged by size:
760 710 285
PmlI CAC'GTG
Cuts at: 0 1752 1755
Size: 1752 3
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 682 714 1177 1209 1755
Size: 682 32 463 32 546
Fragments arranged by size:
682 546 463 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 432 1149 1755
Size: 432 717 606
Fragments arranged by size:
717 606 432
PspOMI G'GGCC_C
Cuts at: 0 555 930 1215 1710 1755
Size: 555 375 285 495 45
Fragments arranged by size:
555 495 375 285 45
PstI C_TGCA'G
Cuts at: 0 895 1755
Size: 895 860
PvuII CAG'CTG
Cuts at: 0 57 1755
Size: 57 1698
SacI G_AGCT'C
Cuts at: 0 1138 1755
Size: 1138 617
SacII CC_GC'GG
Cuts at: 0 126 1624 1755
Size: 126 1498 131
Fragments arranged by size:
1498 131 126
SanDI GG'GwC_CC
Cuts at: 0 1149 1755
Size: 1149 606
SapI GCTCTTCn'nnn_
Cuts at: 0 1255 1282 1755
Size: 1255 27 473
Fragments arranged by size:
1255 473 27
ScaI AGT'ACT
Cuts at: 0 1075 1498 1519 1755
Size: 1075 423 21 236
Fragments arranged by size:
1075 423 236 21
SexAI A'CCwGG_T
Cuts at: 0 1015 1755
Size: 1015 740
SfcI C'TryA_G
Cuts at: 0 891 1755
Size: 891 864
SfoI GGC'GCC
Cuts at: 0 473 524 1647 1755
Size: 473 51 1123 108
Fragments arranged by size:
1123 473 108 51
SmaI CCC'GGG
Cuts at: 0 1460 1755
Size: 1460 295
SmlI C'TyrA_G
Cuts at: 0 1513 1755
Size: 1513 242
SrfI GCCC'GGGC
Cuts at: 0 1460 1755
Size: 1460 295
StuI AGG'CCT
Cuts at: 0 483 719 1755
Size: 483 236 1036
Fragments arranged by size:
1036 483 236
StyI C'CwwG_G
Cuts at: 0 83 335 1649 1755
Size: 83 252 1314 106
Fragments arranged by size:
1314 252 106 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1491 1755
Size: 1491 264
TatI w'GTAC_w
Cuts at: 0 266 1073 1496 1517 1755
Size: 266 807 423 21 238
Fragments arranged by size:
807 423 266 238 21
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1122 1389 1755
Size: 1122 267 366
Fragments arranged by size:
1122 366 267
XmaI C'CCGG_G
Cuts at: 0 1458 1755
Size: 1458 297
Enzymes that do cut:
AarI AccI Acc65I AcuI AleI AlwNI ApaI ApaLI
AvaI BanI BanII BbeI BbsI BglI BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstXI BstYI
BtgI BtgZI DrdI EaeI EagI EarI EcoICRI Eco57MI
EcoO109I HaeII KasI KpnI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NruI NspI PflMI PmlI
PpiI PpuMI PspOMI PstI PvuII SacI SacII SanDI
SapI ScaI SexAI SfcI SfoI SmaI SmlI SrfI
StuI StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AlfI
AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BcgI BciVI BclI BfrBI BglII BmtI BplI
BsaWI BsaXI BseRI BsiWI BsmBI BspEI BsrDI BssSI
BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraI
DraIII EciI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI MfeI MluI MmeI
NdeI NheI NsiI PacI PasI PciI PfoI PmeI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SbfI
SfiI SgfI SgrAI SnaBI SpeI SphI SspI SwaI
TstI Tth111I XbaI XhoI XmnI ZraI