################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AatII AccI AclI AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI AspEI AviII BbrPI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bpu1102I BpuAI BsaBI BsiWI BsmBI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstZ17I CelII ClaI CpoI DraI DrdI Eam1105I EarI Eco47III EcoRI EcoRV EcoT22I FbaI FspI HindIII HpaI Ksp632I MamI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI XbaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### Acc65I 271 GGTACC G^GTACC AccIII 653 TCCGGA T^CCGGA AcsI 940 AAATTC [GA]^AATT[CT] AflII 460 CTTAAG C^TTAAG AlwNI 730 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 558 GGGCCC GGGCC^C ApoI 940 AAATTC [GA]^AATT[CT] Asp700I 706 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 271 GGTACC G^GTACC AspI 343 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1072 CCTAGG C^CTAGG BamHI 1068 GGATCC G^GATCC BbeI 168 GGCGCC GGCGC^C BfrI 460 CTTAAG C^TTAAG BlnI 1072 CCTAGG C^CTAGG BseAI 653 TCCGGA T^CCGGA BsmI 794 GCATTC GAATGC(1/-1) BspEI 653 TCCGGA T^CCGGA BsrBI 594 GAGCGG CCGCTC(-3/-3) BssHII 129 GCGCGC G^CGCGC BstAPI 1274 GCACTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 543 GGTCACC G^GT[ACGT]ACC BstPI 543 GGTCACC G^GT[ACGT]ACC BstYI 1068 GGATCC [GA]^GATC[CT] DraIII 427 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 248 CGGCCG C^GGCCG EclXI 248 CGGCCG C^GGCCG Eco52I 248 CGGCCG C^GGCCG EcoO65I 543 GGTCACC G^GT[ACGT]ACC FseI 902 GGCCGGCC GGCCGG^CC HaeII 168 GGCGCC [GA]GCGC^[CT] HincII 850 GTCAAC GT[CT]^[GA]AC HindII 850 GTCAAC GT[CT]^[GA]AC KasI 168 GGCGCC G^GCGCC KpnI 271 GGTACC GGTAC^C MflI 1068 GGATCC [GA]^GATC[CT] MroI 653 TCCGGA T^CCGGA MslI 795 CATTCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 168 GGCGCC GG^CGCC NcoI 1474 CCATGG C^CATGG NspI 1467 GCATGC [GA]CATG^[CT] PaeR7I 589 CTCGAG C^TCGAG PflFI 343 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 1570 CTGCAG CTGCA^G PvuII 1431 CAGCTG CAG^CTG SacI 1648 GAGCTC GAGCT^C SfoI 168 GGCGCC GGC^GCC SmaI 805 CCCGGG CCC^GGG SphI 1467 GCATGC GCATG^C Tth111I 343 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 589 CTCGAG C^TCGAG XhoII 1068 GGATCC [GA]^GATC[CT] XmaCI 805 CCCGGG C^CCGGG XmaI 805 CCCGGG C^CCGGG XmnI 706 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AlwI 1069 GATCC GGATC(4/5) 1068 GGATC AocI 532 CCTGAGG CC^T[ACGT]AGG 688 CCTCAGG AvaI 589 CTCGAG C^[CT]CG[GA]G 805 CCCGGG BalI 1032 TGGCCA TGG^CCA 1358 TGGCCA BsaAI 157 TACGTG [CT]AC^GT[GA] 718 CACGTA BsoBI 589 CTCGAG C^[CT]CG[GA]G 805 CCCGGG BspMI 1279 ACCTGC ACCTGC(4/8) 1374 GCAGGT BstXI 604 CCAGTCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1046 CCAAGAGCTTGG Bsu36I 532 CCTGAGG CC^T[ACGT]AGG 688 CCTCAGG DpnII 696 GATC ^GATC 1069 GATC DraII 237 AGGTCCC [GA]G^G[ACGT]CC[CT] 1707 GGGACCT Eco81I 532 CCTGAGG CC^T[ACGT]AGG 688 CCTCAGG EcoO109I 237 AGGTCCC [GA]G^G[ACGT]CC[CT] 1707 GGGACCT KspI 314 CCGCGG CCGC^GG 468 CCGCGG MboI 696 GATC ^GATC 1069 GATC MluNI 1032 TGGCCA TGG^CCA 1358 TGGCCA MscI 1032 TGGCCA TGG^CCA 1358 TGGCCA NdeII 696 GATC ^GATC 1069 GATC PflMI 950 CCATCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1748 CCAGGACTTGG PpuMI 237 AGGTCCC [GA]G^G[AT]CC[CT] 1707 GGGACCT SacII 314 CCGCGG CCGC^GG 468 CCGCGG Sau3AI 696 GATC ^GATC 1069 GATC SfcI 84 CTACAG C^T[GA][CT]AG 1570 CTGCAG SmlI 460 CTTAAG C^T[CT][GA]AG 589 CTCGAG TaqI 590 TCGA T^CGA 1665 TCGA Tsp509I 867 AATT ^AATT 941 AATT TthHB8I 590 TCGA T^CGA 1665 TCGA Van91I 950 CCATCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1748 CCAGGACTTGG XcmI 661 CCACTCCAGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1748 CCAGGACTTGGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AspHI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DsaI EaeI EcoNI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NaeI NciI NgoMIV NlaIII NlaIV PleI RsaI Sau96I ScrFI SfaNI StyI TfiI Tru9I TseI Tsp45I TspRI