(Circular) (Six-base) MAPSORT of: pFN21AE6751.seq Check: 6770 from: 1 to: 6484 pFN21AE6751 6484 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4294 278 Size: 53 83 186 936 2758 2468 Fragments arranged by size: 2758 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3827 1937 Size: 1890 4594 Acc65I G'GTAC_C Cuts at: 2276 3805 2276 Size: 1529 4955 AclI AA'CG_TT Cuts at: 1563 4611 4984 1563 Size: 3048 373 3063 Fragments arranged by size: 3063 3048 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2315 2717 4547 5931 6199 1632 Size: 683 402 1830 1384 268 1917 Fragments arranged by size: 1917 1830 1384 683 402 268 AflII C'TTAA_G Cuts at: 820 1017 2465 820 Size: 197 1448 4839 Fragments arranged by size: 4839 1448 197 AflIII A'CryG_T Cuts at: 1203 5383 6360 1203 Size: 4180 977 1327 Fragments arranged by size: 4180 1327 977 AgeI A'CCGG_T Cuts at: 5315 5315 Size: 6484 AhdI GACnn_n'nnGTC Cuts at: 5213 5213 Size: 6484 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6484 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2822 1184 Size: 1638 4846 AlwNI CAG_nnn'CTG Cuts at: 2740 4227 5799 2740 Size: 1487 1572 3425 Fragments arranged by size: 3425 1572 1487 ApaI G_GGCC'C Cuts at: 2567 2567 Size: 6484 ApaLI G'TGCA_C Cuts at: 4541 5697 4541 Size: 1156 5328 ApoI r'AATT_y Cuts at: 1493 2945 3793 4019 4171 6280 1493 Size: 1452 848 226 152 2109 1697 Fragments arranged by size: 2109 1697 1452 848 226 152 AseI AT'TA_AT Cuts at: 160 1032 5038 160 Size: 872 4006 1606 Fragments arranged by size: 4006 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 AvaI C'yCGr_G Cuts at: 1870 1943 2594 2810 3809 1870 Size: 73 651 216 999 4545 Fragments arranged by size: 4545 999 651 216 73 AvrII C'CTAG_G Cuts at: 3077 3077 Size: 6484 BamHI G'GATC_C Cuts at: 3073 3814 4181 3073 Size: 741 367 5376 Fragments arranged by size: 5376 741 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2173 2187 2276 2430 2806 Size: 325 217 627 386 14 89 154 376 Cuts at: 2806 3805 5260 618 Size: 999 1455 1842 Fragments arranged by size: 1842 1455 999 627 386 376 325 217 154 89 14 BanII G_rGCy'C Cuts at: 1391 2102 2567 2996 3503 3657 3803 1391 Size: 711 465 429 507 154 146 4072 Fragments arranged by size: 4072 711 507 465 429 154 146 BbeI G_GCGC'C Cuts at: 2177 2177 Size: 6484 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5513 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4675 4709 1903 Size: 34 50 34 2654 34 3678 Fragments arranged by size: 3678 2654 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4376 5586 4376 Size: 1210 5274 BclI T'GATC_A Cuts at: 1548 1548 Size: 6484 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5095 136 Size: 107 122 71 631 605 3423 1525 Fragments arranged by size: 3423 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6479 6479 Size: 6484 BlpI GC'TnA_GC Cuts at: 3901 3901 Size: 6484 Bme1580I G_kGCm'C Cuts at: 1790 2190 2567 2811 4545 5701 1790 Size: 400 377 244 1734 1156 2573 Fragments arranged by size: 2573 1734 1156 400 377 244 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6484 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2647 2803 3157 Size: 416 53 83 399 24 1230 156 354 Cuts at: 3157 3497 3502 3732 4215 5173 442 Size: 340 5 230 483 958 1753 Fragments arranged by size: 1753 1230 958 483 416 399 354 340 230 156 83 53 24 5 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6484 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2087 2654 3443 5144 1239 Size: 848 567 789 1701 2579 Fragments arranged by size: 2579 1701 848 789 567 Bpu10I CC'TnA_GC Cuts at: 3330 3330 Size: 6484 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4609 5474 5772 4609 Size: 865 298 5321 Fragments arranged by size: 5321 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3224 3254 3631 5147 882 Size: 617 1725 30 377 1516 2219 Fragments arranged by size: 2219 1725 1516 617 377 30 BsaAI yAC'GTr Cuts at: 493 1204 2164 2725 4275 493 Size: 711 960 561 1550 2702 Fragments arranged by size: 2702 1550 960 711 561 BsaBI GATnn'nnATC Cuts at: 6478 6478 Size: 6484 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2174 2214 2493 3138 Size: 53 83 186 936 641 40 279 645 Cuts at: 3138 4291 4673 5308 275 Size: 1153 382 635 1451 Fragments arranged by size: 1451 1153 936 645 641 635 382 279 186 83 53 40 BsaWI w'CCGG_w Cuts at: 2658 2803 3777 4917 5315 5589 5736 6147 2658 Size: 145 974 1140 398 274 147 411 2995 Fragments arranged by size: 2995 1140 974 411 398 274 147 145 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3416 3446 1178 Size: 30 165 30 2013 30 4216 Fragments arranged by size: 4216 2013 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2762 3655 3718 1186 Size: 1576 893 63 3952 Fragments arranged by size: 3952 1576 893 63 BseYI C'CCAG_C Cuts at: 2246 2553 3268 3520 3619 3709 5687 6183 2246 Size: 307 715 252 99 90 1978 496 2547 Fragments arranged by size: 2547 1978 715 496 307 252 99 90 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2383 2465 3262 1163 Size: 573 647 82 797 4385 Fragments arranged by size: 4385 797 647 573 82 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2256 2337 2350 3934 4695 5723 Size: 275 186 255 81 13 1584 761 1028 Cuts at: 5723 1540 Size: 2301 Fragments arranged by size: 2301 1584 1028 761 275 255 186 81 13 BsiHKAI G_wGCw'C Cuts at: 2289 3331 3657 4545 4630 5701 2289 Size: 1042 326 888 85 1071 3072 Fragments arranged by size: 3072 1071 1042 888 326 85 BsmI GAATG_Cn' Cuts at: 2799 3995 4088 2799 Size: 1196 93 5195 Fragments arranged by size: 5195 1196 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2102 2190 2289 2567 2811 2996 3331 Size: 399 312 88 99 278 244 185 335 Cuts at: 3331 3503 3657 3803 4545 4630 5701 1391 Size: 172 154 146 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 335 312 278 244 185 172 154 146 99 88 85 BspEI T'CCGG_A Cuts at: 2658 6147 2658 Size: 3489 2995 BspHI T'CATG_A Cuts at: 4373 6103 4373 Size: 1730 4754 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3293 3370 3839 844 Size: 2449 77 469 3489 Fragments arranged by size: 3489 2449 469 77 BsrBI CCG'CTC Cuts at: 1394 1823 2601 4371 1394 Size: 429 778 1770 3507 Fragments arranged by size: 3507 1770 778 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4979 5153 65 Size: 1183 3731 174 1396 Fragments arranged by size: 3731 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2111 2387 2908 2984 5128 5315 1700 Size: 110 301 276 521 76 2144 187 2869 Fragments arranged by size: 2869 2144 521 301 276 187 110 76 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6484 BssHII G'CGCG_C Cuts at: 1925 2134 1925 Size: 209 6275 BssSI C'ACGA_G Cuts at: 4544 5556 4544 Size: 1012 5472 BstAPI GCAn_nnn'nTGC Cuts at: 794 3285 794 Size: 2491 3993 BstBI TT'CG_AA Cuts at: 5288 5288 Size: 6484 BstEII G'GTnAC_C Cuts at: 1184 2548 1184 Size: 1364 5120 BstXI CCAn_nnnn'nTGG Cuts at: 2616 3058 2616 Size: 442 6042 BstYI r'GATC_y Cuts at: 1972 3073 3814 4181 4566 4583 6035 6121 6133 Size: 1101 741 367 385 17 1452 86 12 Cuts at: 6133 6479 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1101 741 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2538 2694 2538 Size: 156 6328 BtgI C'CryG_G Cuts at: 513 1065 2319 2418 2473 3102 3479 513 Size: 552 1254 99 55 629 377 3518 Fragments arranged by size: 3518 1254 629 552 377 99 55 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2130 487 Size: 683 56 904 4841 Fragments arranged by size: 4841 904 683 56 BtsI GCAGTG_nn' Cuts at: 3234 3366 4000 4794 4814 3234 Size: 132 634 794 20 4904 Fragments arranged by size: 4904 794 634 132 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6484 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6449 DraI TTT'AAA Cuts at: 3788 3788 Size: 6484 DraIII CAC_nnn'GTG Cuts at: 2437 2437 Size: 6484 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5491 809 Size: 507 4175 1802 Fragments arranged by size: 4175 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2253 2906 2910 Size: 54 1425 50 275 18 423 653 4 Cuts at: 2910 3037 3363 3931 4820 6449 8 Size: 127 326 568 889 1629 43 Fragments arranged by size: 1629 1425 889 653 568 423 326 275 127 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2253 3931 1537 Size: 275 441 1678 4090 Fragments arranged by size: 4090 1678 441 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4414 1353 Size: 3061 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2015 2225 5071 5445 5591 387 Size: 1064 138 426 210 2846 374 146 1280 Fragments arranged by size: 2846 1280 1064 426 374 210 146 138 EcoICRI GAG'CTC Cuts at: 3655 3655 Size: 6484 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2315 2654 2717 3443 4547 5144 Size: 393 455 228 339 63 726 1104 597 Cuts at: 5144 5931 6199 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1104 787 726 597 455 393 339 268 228 63 EcoNI CCTnn'n_nnAGG Cuts at: 2537 3131 3637 3696 2537 Size: 594 506 59 5325 Fragments arranged by size: 5325 594 506 59 EcoO109I rG'GnC_Cy Cuts at: 2243 3713 2243 Size: 1470 5014 EcoRI G'AATT_C Cuts at: 3793 4171 3793 Size: 378 6106 FseI GG_CCGG'CC Cuts at: 2912 2912 Size: 6484 FspI TGC'GCA Cuts at: 4990 4990 Size: 6484 HaeII r_GCGC'y Cuts at: 1678 2177 5631 1678 Size: 499 3454 2531 Fragments arranged by size: 3454 2531 499 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2339 2371 2590 2622 2882 Size: 32 135 32 767 32 219 32 260 Cuts at: 2882 2914 3416 3448 5125 5157 5199 5231 1373 Size: 32 502 32 1677 32 42 32 2626 Fragments arranged by size: 2626 1677 767 502 260 219 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2857 3828 4074 669 Size: 988 281 919 971 246 3079 Fragments arranged by size: 3079 988 971 919 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6484 HpaI GTT'AAC Cuts at: 4074 4074 Size: 6484 KasI G'GCGC_C Cuts at: 2173 2173 Size: 6484 KpnI G_GTAC'C Cuts at: 2280 3809 2280 Size: 1529 4955 MfeI C'AATT_G Cuts at: 4083 4083 Size: 6484 MluI A'CGCG_T Cuts at: 6360 6360 Size: 6484 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2544 5598 5782 1305 Size: 1239 3054 184 2007 Fragments arranged by size: 3054 2007 1239 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3039 3365 10 Size: 54 1425 343 1207 326 3129 Fragments arranged by size: 3129 1425 1207 343 326 54 MslI CAynn'nnrTG Cuts at: 518 1166 2804 4442 4801 4960 518 Size: 648 1638 1638 359 159 2042 Fragments arranged by size: 2042 1638 1638 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2321 2475 2674 3438 3900 4577 5725 5970 Size: 715 154 199 764 462 677 1148 245 Cuts at: 5970 1606 Size: 2120 Fragments arranged by size: 2120 1148 764 715 677 462 245 199 154 NaeI GCC'GGC Cuts at: 2113 2910 2986 2113 Size: 797 76 5611 Fragments arranged by size: 5611 797 76 NarI GG'CG_CC Cuts at: 2174 2174 Size: 6484 NcoI C'CATG_G Cuts at: 513 1065 3479 513 Size: 552 2414 3518 Fragments arranged by size: 3518 2414 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6484 NgoMIV G'CCGG_C Cuts at: 2111 2908 2984 2111 Size: 797 76 5611 Fragments arranged by size: 5611 797 76 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6484 NotI GC'GGCC_GC Cuts at: 3931 3931 Size: 6484 NspI r_CATG'y Cuts at: 1132 1229 3476 3842 5387 1132 Size: 97 2247 366 1545 2229 Fragments arranged by size: 2247 2229 1545 366 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6484 PciI A'CATG_T Cuts at: 5383 5383 Size: 6484 PflMI CCAn_nnn'nTGG Cuts at: 1108 2961 3759 1108 Size: 1853 798 3833 Fragments arranged by size: 3833 1853 798 PfoI T'CCnGG_A Cuts at: 2064 2379 2615 3752 2064 Size: 315 236 1137 4796 Fragments arranged by size: 4796 1137 315 236 PmeI GTTT'AAAC Cuts at: 3788 3788 Size: 6484 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4553 4585 6066 6098 4553 Size: 32 1481 32 4939 Fragments arranged by size: 4939 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2243 3713 2243 Size: 1470 5014 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6484 PsiI TTA'TAA Cuts at: 4054 4054 Size: 6484 PspOMI G'GGCC_C Cuts at: 2563 2563 Size: 6484 PstI C_TGCA'G Cuts at: 830 3579 3836 830 Size: 2749 257 3478 Fragments arranged by size: 3478 2749 257 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6484 PvuII CAG'CTG Cuts at: 3438 3438 Size: 6484 RsrII CG'GwC_CG Cuts at: 3780 3780 Size: 6484 SacI G_AGCT'C Cuts at: 3657 3657 Size: 6484 SacII CC_GC'GG Cuts at: 2322 2476 2322 Size: 154 6330 SalI G'TCGA_C Cuts at: 1936 3826 1936 Size: 1890 4594 SbfI CC_TGCA'GG Cuts at: 3836 3836 Size: 6484 ScaI AGT'ACT Cuts at: 4732 6444 4732 Size: 1712 4772 SfcI C'TryA_G Cuts at: 826 1045 2089 3575 3832 4967 5648 5839 826 Size: 219 1044 1486 257 1135 681 191 1471 Fragments arranged by size: 1486 1471 1135 1044 681 257 219 191 SfoI GGC'GCC Cuts at: 2175 2175 Size: 6484 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6484 SmaI CCC'GGG Cuts at: 1872 2812 3811 1872 Size: 940 999 4545 Fragments arranged by size: 4545 999 940 SmlI C'TyrA_G Cuts at: 820 1017 1943 2465 2594 4588 5489 5751 820 Size: 197 926 522 129 1994 901 262 1553 Fragments arranged by size: 1994 1553 926 901 522 262 197 129 SnaBI TAC'GTA Cuts at: 493 4275 493 Size: 3782 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6484 SphI G_CATG'C Cuts at: 1132 3476 3842 1132 Size: 2344 366 3774 Fragments arranged by size: 3774 2344 366 SspI AAT'ATT Cuts at: 5 52 4408 5 Size: 47 4356 2081 Fragments arranged by size: 4356 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6484 StyI C'CwwG_G Cuts at: 513 1065 2544 2622 3077 3210 3479 3543 3923 Size: 552 1479 78 455 133 269 64 380 Cuts at: 3923 513 Size: 3074 Fragments arranged by size: 3074 1479 552 455 380 269 133 78 64 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2167 2351 2364 2567 4511 4528 4681 Size: 1255 587 184 13 203 1944 17 153 Cuts at: 4681 4866 5311 325 Size: 185 445 1498 Fragments arranged by size: 1944 1498 1255 587 445 203 185 184 153 17 13 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2193 4730 6442 Size: 274 80 33 51 1443 216 2537 1712 Cuts at: 6442 96 Size: 138 Fragments arranged by size: 2537 1712 1443 274 216 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3019 3051 6444 6476 3019 Size: 32 3393 32 3027 Fragments arranged by size: 3393 3027 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2351 1316 Size: 1035 5449 XbaI T'CTAG_A Cuts at: 3820 3820 Size: 6484 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2673 3760 2673 Size: 1087 5397 XhoI C'TCGA_G Cuts at: 1943 2594 1943 Size: 651 5833 XmaI C'CCGG_G Cuts at: 1870 2810 3809 1870 Size: 940 999 4545 Fragments arranged by size: 4545 999 940 XmnI GAAnn'nnTTC Cuts at: 2715 4613 2715 Size: 1898 4586 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4292 276 Size: 53 83 186 936 2758 2468 Fragments arranged by size: 2758 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoRV FalI FspAI NruI NsiI PacI PmlI PspXI PsrI SanDI SapI SexAI SfiI SrfI SwaI