(Linear) (Six-base) MAPSORT of: KIEE6751.seq Check: 6584 from: 1 to: 1779 KIEE6751.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. Acc65I G'GTAC_C Cuts at: 0 271 1779 Size: 271 1508 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 310 712 1779 Size: 310 402 1067 Fragments arranged by size: 1067 402 310 AflII C'TTAA_G Cuts at: 0 460 1779 Size: 460 1319 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 817 1779 Size: 817 962 AlwNI CAG_nnn'CTG Cuts at: 0 735 1779 Size: 735 1044 ApaI G_GGCC'C Cuts at: 0 562 1779 Size: 562 1217 ApoI r'AATT_y Cuts at: 0 940 1779 Size: 940 839 AvaI C'yCGr_G Cuts at: 0 589 805 1779 Size: 589 216 974 Fragments arranged by size: 974 589 216 AvrII C'CTAG_G Cuts at: 0 1072 1779 Size: 1072 707 BamHI G'GATC_C Cuts at: 0 1068 1779 Size: 1068 711 BanI G'GyrC_C Cuts at: 0 168 182 271 425 801 1779 Size: 168 14 89 154 376 978 Fragments arranged by size: 978 376 168 154 89 14 BanII G_rGCy'C Cuts at: 0 97 562 991 1498 1652 1779 Size: 97 465 429 507 154 127 Fragments arranged by size: 507 465 429 154 127 97 BbeI G_GCGC'C Cuts at: 0 172 1779 Size: 172 1607 Bme1580I G_kGCm'C Cuts at: 0 185 562 806 1779 Size: 185 377 244 973 Fragments arranged by size: 973 377 244 185 BmrI ACTGGGnnnn_n' Cuts at: 0 642 798 1152 1492 1497 1727 1779 Size: 642 156 354 340 5 230 52 Fragments arranged by size: 642 354 340 230 156 52 5 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 82 649 1438 1779 Size: 82 567 789 341 Fragments arranged by size: 789 567 341 82 Bpu10I CC'TnA_GC Cuts at: 0 1325 1779 Size: 1325 454 BsaI GGTCTCn'nnnn_ Cuts at: 0 1219 1249 1626 1779 Size: 1219 30 377 153 Fragments arranged by size: 1219 377 153 30 BsaAI yAC'GTr Cuts at: 0 159 720 1779 Size: 159 561 1059 Fragments arranged by size: 1059 561 159 BsaHI Gr'CG_yC Cuts at: 0 169 209 488 1133 1779 Size: 169 40 279 645 646 Fragments arranged by size: 646 645 279 169 40 BsaWI w'CCGG_w Cuts at: 0 653 798 1772 1779 Size: 653 145 974 7 Fragments arranged by size: 974 653 145 7 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1411 1441 1779 Size: 1411 30 338 Fragments arranged by size: 1411 338 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 757 1650 1713 1779 Size: 757 893 63 66 Fragments arranged by size: 893 757 66 63 BseYI C'CCAG_C Cuts at: 0 241 548 1263 1515 1614 1704 1779 Size: 241 307 715 252 99 90 75 Fragments arranged by size: 715 307 252 241 99 90 75 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 378 460 1257 1779 Size: 378 82 797 522 Fragments arranged by size: 797 522 378 82 BsiEI CG_ry'CG Cuts at: 0 251 332 345 1779 Size: 251 81 13 1434 Fragments arranged by size: 1434 251 81 13 BsiHKAI G_wGCw'C Cuts at: 0 284 1326 1652 1779 Size: 284 1042 326 127 Fragments arranged by size: 1042 326 284 127 BsmI GAATG_Cn' Cuts at: 0 794 1779 Size: 794 985 Bsp1286I G_dGCh'C Cuts at: 0 97 185 284 562 806 991 1326 1498 Size: 97 88 99 278 244 185 335 172 Cuts at: 1498 1652 1779 Size: 154 127 Fragments arranged by size: 335 278 244 185 172 154 127 99 97 88 BspEI T'CCGG_A Cuts at: 0 653 1779 Size: 653 1126 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1288 1365 1779 Size: 1288 77 414 Fragments arranged by size: 1288 414 77 BsrBI CCG'CTC Cuts at: 0 596 1779 Size: 596 1183 BsrFI r'CCGG_y Cuts at: 0 106 382 903 979 1779 Size: 106 276 521 76 800 Fragments arranged by size: 800 521 276 106 76 BssHII G'CGCG_C Cuts at: 0 129 1779 Size: 129 1650 BstAPI GCAn_nnn'nTGC Cuts at: 0 1280 1779 Size: 1280 499 BstEII G'GTnAC_C Cuts at: 0 543 1779 Size: 543 1236 BstXI CCAn_nnnn'nTGG Cuts at: 0 611 1053 1779 Size: 611 442 726 Fragments arranged by size: 726 611 442 BstYI r'GATC_y Cuts at: 0 1068 1779 Size: 1068 711 Bsu36I CC'TnA_GG Cuts at: 0 533 689 1779 Size: 533 156 1090 Fragments arranged by size: 1090 533 156 BtgI C'CryG_G Cuts at: 0 314 413 468 1097 1474 1779 Size: 314 99 55 629 377 305 Fragments arranged by size: 629 377 314 305 99 55 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 125 1779 Size: 125 1654 BtsI GCAGTG_nn' Cuts at: 0 1229 1361 1779 Size: 1229 132 418 Fragments arranged by size: 1229 418 132 DraIII CAC_nnn'GTG Cuts at: 0 432 1779 Size: 432 1347 EaeI y'GGCC_r Cuts at: 0 248 901 905 1032 1358 1779 Size: 248 653 4 127 326 421 Fragments arranged by size: 653 421 326 248 127 4 EagI C'GGCC_G Cuts at: 0 248 1779 Size: 248 1531 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 10 220 1779 Size: 10 210 1559 Fragments arranged by size: 1559 210 10 EcoICRI GAG'CTC Cuts at: 0 1650 1779 Size: 1650 129 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 82 310 649 712 1438 1779 Size: 82 228 339 63 726 341 Fragments arranged by size: 726 341 339 228 82 63 EcoNI CCTnn'n_nnAGG Cuts at: 0 532 1126 1632 1691 1779 Size: 532 594 506 59 88 Fragments arranged by size: 594 532 506 88 59 EcoO109I rG'GnC_Cy Cuts at: 0 238 1708 1779 Size: 238 1470 71 Fragments arranged by size: 1470 238 71 FseI GG_CCGG'CC Cuts at: 0 907 1779 Size: 907 872 HaeII r_GCGC'y Cuts at: 0 172 1779 Size: 172 1607 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 334 366 585 617 877 909 1411 1443 Size: 334 32 219 32 260 32 502 32 Cuts at: 1443 1779 Size: 336 Fragments arranged by size: 502 336 334 260 219 32 32 32 32 HincII GTy'rAC Cuts at: 0 852 1779 Size: 852 927 KasI G'GCGC_C Cuts at: 0 168 1779 Size: 168 1611 KpnI G_GTAC'C Cuts at: 0 275 1779 Size: 275 1504 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 539 1779 Size: 539 1240 MscI TGG'CCA Cuts at: 0 1034 1360 1779 Size: 1034 326 419 Fragments arranged by size: 1034 419 326 MslI CAynn'nnrTG Cuts at: 0 799 1779 Size: 799 980 MspA1I CmG'CkG Cuts at: 0 316 470 669 1433 1779 Size: 316 154 199 764 346 Fragments arranged by size: 764 346 316 199 154 NaeI GCC'GGC Cuts at: 0 108 905 981 1779 Size: 108 797 76 798 Fragments arranged by size: 798 797 108 76 NarI GG'CG_CC Cuts at: 0 169 1779 Size: 169 1610 NcoI C'CATG_G Cuts at: 0 1474 1779 Size: 1474 305 NgoMIV G'CCGG_C Cuts at: 0 106 903 979 1779 Size: 106 797 76 800 Fragments arranged by size: 800 797 106 76 NspI r_CATG'y Cuts at: 0 1471 1779 Size: 1471 308 PflMI CCAn_nnn'nTGG Cuts at: 0 956 1754 1779 Size: 956 798 25 Fragments arranged by size: 956 798 25 PfoI T'CCnGG_A Cuts at: 0 59 374 610 1747 1779 Size: 59 315 236 1137 32 Fragments arranged by size: 1137 315 236 59 32 PpuMI rG'GwC_Cy Cuts at: 0 238 1708 1779 Size: 238 1470 71 Fragments arranged by size: 1470 238 71 PspOMI G'GGCC_C Cuts at: 0 558 1779 Size: 558 1221 PstI C_TGCA'G Cuts at: 0 1574 1779 Size: 1574 205 PvuII CAG'CTG Cuts at: 0 1433 1779 Size: 1433 346 SacI G_AGCT'C Cuts at: 0 1652 1779 Size: 1652 127 SacII CC_GC'GG Cuts at: 0 317 471 1779 Size: 317 154 1308 Fragments arranged by size: 1308 317 154 SfcI C'TryA_G Cuts at: 0 84 1570 1779 Size: 84 1486 209 Fragments arranged by size: 1486 209 84 SfoI GGC'GCC Cuts at: 0 170 1779 Size: 170 1609 SmaI CCC'GGG Cuts at: 0 807 1779 Size: 807 972 SmlI C'TyrA_G Cuts at: 0 460 589 1779 Size: 460 129 1190 Fragments arranged by size: 1190 460 129 SphI G_CATG'C Cuts at: 0 1471 1779 Size: 1471 308 StyI C'CwwG_G Cuts at: 0 539 617 1072 1205 1474 1538 1779 Size: 539 78 455 133 269 64 241 Fragments arranged by size: 539 455 269 241 133 78 64 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 162 346 359 562 1779 Size: 162 184 13 203 1217 Fragments arranged by size: 1217 203 184 162 13 TatI w'GTAC_w Cuts at: 0 188 1779 Size: 188 1591 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1014 1046 1779 Size: 1014 32 733 Fragments arranged by size: 1014 733 32 Tth111I GACn'n_nGTC Cuts at: 0 346 1779 Size: 346 1433 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 668 1755 1779 Size: 668 1087 24 Fragments arranged by size: 1087 668 24 XhoI C'TCGA_G Cuts at: 0 589 1779 Size: 589 1190 XmaI C'CCGG_G Cuts at: 0 805 1779 Size: 805 974 XmnI GAAnn'nnTTC Cuts at: 0 710 1779 Size: 710 1069 Enzymes that do cut: Acc65I AcuI AflII AlfI AlwNI ApaI ApoI AvaI AvrII BamHI BanI BanII BbeI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrFI BssHII BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SacII SfcI SfoI SmaI SmlI SphI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflIII AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI BaeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI BpuEI BsaBI BsiWI BsmBI BspHI BsrDI BsrGI BssSI BstBI BstZ17I ClaI CspCI DraI DrdI EarI EcoRI EcoRV FalI FspI FspAI HindIII HpaI MfeI MluI NdeI NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI XbaI ZraI