################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BciVI BfrI BlnI BlpI BmyI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoRI FseI FspI HgaI Hin1I HincII HindII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI ScaI SexAI SfoI SfuI SgrAI SmlI SpeI SphI SspBI SwaI Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Aor51HI 128 AGCGCT AGC^GCT AvaI 42 CCCGGG C^[CT]CG[GA]G BalI 261 TGGCCA TGG^CCA BbvCI 120 GCTGAGG CCTCAGC(-5/-2) BcgI 200 GCATTGCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 92 TGATCA T^GATCA BfaI 650 CTAG C^TAG BglI 235 GCCTGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 470 CTGGAG CTGGAG(16/14) BsaAI 493 TACGTA [CT]AC^GT[GA] BseRI 250 GAGGAG GAGGAG(10/8) BsgI 1016 CTGCAC GTGCAG(16/14) BsiWI 495 CGTACG C^GTACG BsmAI 1359 GAGAC GTCTC(1/5) BsoBI 42 CCCGGG C^[CT]CG[GA]G BsrBI 24 GAGCGG CCGCTC(-3/-3) BstAPI 1018 GCACAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1240 GGTAACC G^GT[ACGT]ACC BstPI 1240 GGTAACC G^GT[ACGT]ACC CfoI 129 GCGC GCG^C DsaI 245 CCATGG C^C[GA][CT]GG EaeI 261 TGGCCA [CT]^GGCC[GA] Eco47III 128 AGCGCT AGC^GCT EcoO65I 1240 GGTAACC G^GT[ACGT]ACC EcoRV 433 GATATC GAT^ATC EcoT22I 256 ATGCAT ATGCA^T FbaI 92 TGATCA T^GATCA HaeII 128 AGCGCT [GA]GCGC^[CT] HapII 43 CCGG C^CGG HhaI 129 GCGC GCG^C HinP1I 129 GCGC G^CGC HindIII 632 AAGCTT A^AGCTT HpaII 43 CCGG C^CGG MaeI 650 CTAG C^TAG MluNI 261 TGGCCA TGG^CCA MscI 261 TGGCCA TGG^CCA MspI 43 CCGG C^CGG NcoI 245 CCATGG C^CATGG NsiI 256 ATGCAT ATGCA^T PstI 237 CTGCAG CTGCA^G SbfI 236 CCTGCAGG CCTGCA^GG SfcI 237 CTGCAG C^T[GA][CT]AG SfiI 234 GGCCTGCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 42 CCCGGG CCC^GGG SnaBI 493 TACGTA TAC^GTA Sse8387I 236 CCTGCAGG CCTGCA^GG SspI 815 AATATT AAT^ATT StuI 233 AGGCCT AGG^CCT XmaCI 42 CCCGGG C^CCGGG XmaI 42 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcsI 415 AAATTT [GA]^AATT[CT] 685 GAATTT AlwI 835 GATCC GGATC(4/5) 1087 GGATC ApoI 415 AAATTT [GA]^AATT[CT] 685 GAATTT BcnI 42 CCCGG CC^[GC]GG 43 CCGGG BglII 402 AGATCT A^GATCT 981 AGATCT BsiYI 42 CCCGGGCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 714 CCGAGGCCAGG BslI 42 CCCGGGCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 714 CCGAGGCCAGG BsrI 469 ACTGG ACTGG(1/-1) 264 CCAGT BstF5I 150 CATCC GGATG(2/0) 1195 CATCC Cfr13I 242 GGGCC G^G[ACGT]CC 274 GGCCC FokI 150 CATCC GGATG(9/13) 1195 CATCC HphI 1356 GGTGA GGTGA(8/7) 333 TCACC MaeII 494 ACGT A^CGT 1211 ACGT MslI 159 CATCTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 334 CACCGAGATG NciI 42 CCCGG CC^[GC]GG 43 CCGGG NlaIV 274 GGCCCC GG[ACGT]^[ACGT]CC 935 GGAACC PleI 112 GAGTC GAGTC(4/5) 190 GAGTC Sau96I 242 GGGCC G^G[ACGT]CC 274 GGCCC Tsp45I 332 GTCAC ^GT[GC]AC 466 GTGAC TspRI 265 CAGTG CA[GC]TG[ACGT][ACGT]^ 768 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AciI AfaI AluI BbvI BsaJI BsmFI BsrDI BssKI BstNI BstYI Cac8I DdeI DpnII EcoRII EcoT14I FauI Fnu4HI HaeIII HinfI ItaI MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII RsaI Sau3AI ScrFI SfaNI StyI TaqI TfiI Tru9I TseI Tsp509I TthHB8I XhoII