(Circular) (Six-base) MAPSORT of: pFN21AE5623.seq Check: 5363 from: 1 to: 6127 pFN21AE5623 6127 bp With 182 enzymes: SgfI * December 8, 2014 13:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3937 278 Size: 53 83 186 936 2401 2468 Fragments arranged by size: 2468 2401 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3470 1937 Size: 1533 4594 Acc65I G'GTAC_C Cuts at: 3448 3448 Size: 6127 AclI AA'CG_TT Cuts at: 1563 4254 4627 1563 Size: 2691 373 3063 Fragments arranged by size: 3063 2691 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2796 4190 5574 5842 1632 Size: 1164 1394 1384 268 1917 Fragments arranged by size: 1917 1394 1384 1164 268 AfeI AGC'GCT Cuts at: 2135 2135 Size: 6127 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5930 AflIII A'CryG_T Cuts at: 1203 5026 6003 1203 Size: 3823 977 1327 Fragments arranged by size: 3823 1327 977 AgeI A'CCGG_T Cuts at: 4958 4958 Size: 6127 AhdI GACnn_n'nnGTC Cuts at: 4856 4856 Size: 6127 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6127 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6127 AlwNI CAG_nnn'CTG Cuts at: 3870 5442 3870 Size: 1572 4555 ApaLI G'TGCA_C Cuts at: 4184 5340 4184 Size: 1156 4971 ApoI r'AATT_y Cuts at: 1493 2420 2690 3436 3662 3814 5923 1493 Size: 927 270 746 226 152 2109 1697 Fragments arranged by size: 2109 1697 927 746 270 226 152 AseI AT'TA_AT Cuts at: 160 1032 4681 160 Size: 872 3649 1606 Fragments arranged by size: 3649 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 AvaI C'yCGr_G Cuts at: 1870 1943 2047 3452 1870 Size: 73 104 1405 4545 Fragments arranged by size: 4545 1405 104 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2375 2408 2375 Size: 33 6094 BamHI G'GATC_C Cuts at: 3457 3824 3457 Size: 367 5760 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3448 4903 618 Size: 325 217 627 1661 1455 1842 Fragments arranged by size: 1842 1661 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3446 1391 Size: 2055 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5156 BbvCI CC'TCA_GC Cuts at: 2126 2126 Size: 6127 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2194 2228 4318 4352 1903 Size: 34 257 34 2090 34 3678 Fragments arranged by size: 3678 2090 257 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4019 5229 4019 Size: 1210 4917 BclI T'GATC_A Cuts at: 1548 2097 1548 Size: 549 5578 BfrBI ATG'CAT Cuts at: 2263 2263 Size: 6127 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2246 4738 136 Size: 107 122 71 631 605 574 2492 1525 Fragments arranged by size: 2492 1525 631 605 574 122 107 71 BglII A'GATC_T Cuts at: 2407 2986 6122 2407 Size: 579 3136 2412 Fragments arranged by size: 3136 2412 579 BlpI GC'TnA_GC Cuts at: 3544 3544 Size: 6127 Bme1580I G_kGCm'C Cuts at: 1790 4188 5344 1790 Size: 2398 1156 2573 Fragments arranged by size: 2573 2398 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6127 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3858 4816 442 Size: 416 53 83 399 24 2441 958 1753 Fragments arranged by size: 2441 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6127 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2155 2155 Size: 6127 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2496 4787 1239 Size: 1257 2291 2579 Fragments arranged by size: 2579 2291 1257 Bpu10I CC'TnA_GC Cuts at: 2126 2126 Size: 6127 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4252 5117 5415 4252 Size: 865 298 4964 Fragments arranged by size: 4964 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4790 882 Size: 617 3291 2219 Fragments arranged by size: 3291 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2500 3918 493 Size: 711 1296 1418 2702 Fragments arranged by size: 2702 1418 1296 711 BsaBI GATnn'nnATC Cuts at: 6121 6121 Size: 6127 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3934 4316 4951 275 Size: 53 83 186 936 2401 382 635 1451 Fragments arranged by size: 2401 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4560 4958 5232 5379 5790 4560 Size: 398 274 147 411 4897 Fragments arranged by size: 4897 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5902 Fragments arranged by size: 5902 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2270 1186 Size: 1084 5043 BseYI C'CCAG_C Cuts at: 5330 5826 5330 Size: 496 5631 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3006 1163 Size: 573 1270 4284 Fragments arranged by size: 4284 1270 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3577 4338 5366 1540 Size: 275 186 1576 761 1028 2301 Fragments arranged by size: 2301 1576 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4188 4273 5344 4188 Size: 85 1071 4971 Fragments arranged by size: 4971 1071 85 BsiWI C'GTAC_G Cuts at: 2500 2500 Size: 6127 BsmI GAATG_Cn' Cuts at: 3638 3731 3638 Size: 93 6034 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3446 4188 4273 5344 1391 Size: 399 1656 742 85 1071 2174 Fragments arranged by size: 2174 1656 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5790 5790 Size: 6127 BspHI T'CATG_A Cuts at: 4016 5746 4016 Size: 1730 4397 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3482 844 Size: 2638 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2031 4014 1394 Size: 429 208 1983 3507 Fragments arranged by size: 3507 1983 429 208 BsrDI GCAATG_nn' Cuts at: 65 1248 2205 2310 3327 4622 4796 65 Size: 1183 957 105 1017 1295 174 1396 Fragments arranged by size: 1396 1295 1183 1017 957 174 105 BsrFI r'CCGG_y Cuts at: 1700 1810 4771 4958 1700 Size: 110 2961 187 2869 Fragments arranged by size: 2961 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6127 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6127 BssSI C'ACGA_G Cuts at: 4187 5199 4187 Size: 1012 5115 BstAPI GCAn_nnn'nTGC Cuts at: 794 3029 794 Size: 2235 3892 BstBI TT'CG_AA Cuts at: 4931 4931 Size: 6127 BstEII G'GTnAC_C Cuts at: 1184 3245 1184 Size: 2061 4066 BstYI r'GATC_y Cuts at: 1972 2407 2839 2986 3457 3824 4209 4226 5678 Size: 435 432 147 471 367 385 17 1452 Cuts at: 5678 5764 5776 6122 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 471 435 432 385 367 346 147 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2250 513 Size: 552 939 246 4390 Fragments arranged by size: 4390 939 552 246 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5388 Fragments arranged by size: 5388 683 56 BtsI GCAGTG_nn' Cuts at: 3643 4437 4457 3643 Size: 794 20 5313 Fragments arranged by size: 5313 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6127 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6092 DraI TTT'AAA Cuts at: 3431 3431 Size: 6127 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5134 809 Size: 507 3818 1802 Fragments arranged by size: 3818 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2266 3574 4463 Size: 54 1425 50 275 18 436 1308 889 Cuts at: 4463 6092 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1308 889 436 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3574 1537 Size: 275 1762 4090 Fragments arranged by size: 4090 1762 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4057 1353 Size: 2704 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2033 4714 5088 5234 387 Size: 1064 138 444 2681 374 146 1280 Fragments arranged by size: 2681 1280 1064 444 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2496 2796 4190 4787 5574 5842 1239 Size: 393 864 300 1394 597 787 268 1524 Fragments arranged by size: 1524 1394 864 787 597 393 300 268 EcoRI G'AATT_C Cuts at: 3436 3814 3436 Size: 378 5749 EcoRV GAT'ATC Cuts at: 2440 2440 Size: 6127 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2784 2801 3194 3281 2784 Size: 17 393 87 5630 Fragments arranged by size: 5630 393 87 17 FspI TGC'GCA Cuts at: 4633 4633 Size: 6127 HaeII r_GCGC'y Cuts at: 1678 2137 5274 1678 Size: 459 3137 2531 Fragments arranged by size: 3137 2531 459 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4768 4800 4842 4874 1373 Size: 32 135 32 3196 32 42 32 2626 Fragments arranged by size: 3196 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3471 3717 669 Size: 988 281 1533 246 3079 Fragments arranged by size: 3079 1533 988 281 246 HindIII A'AGCT_T Cuts at: 748 2637 748 Size: 1889 4238 HpaI GTT'AAC Cuts at: 3717 3717 Size: 6127 KpnI G_GTAC'C Cuts at: 3452 3452 Size: 6127 MfeI C'AATT_G Cuts at: 3726 3726 Size: 6127 MluI A'CGCG_T Cuts at: 6003 6003 Size: 6127 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5241 5425 1305 Size: 3936 184 2007 Fragments arranged by size: 3936 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2268 10 Size: 54 1425 343 436 3869 Fragments arranged by size: 3869 1425 436 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2168 2343 4085 4444 4603 518 Size: 648 1002 175 1742 359 159 2042 Fragments arranged by size: 2042 1742 1002 648 359 175 159 MspA1I CmG'CkG Cuts at: 1606 3543 4220 5368 5613 1606 Size: 1937 677 1148 245 2120 Fragments arranged by size: 2120 1937 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2250 513 Size: 552 939 246 4390 Fragments arranged by size: 4390 939 552 246 NdeI CA'TA_TG Cuts at: 387 387 Size: 6127 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6127 NotI GC'GGCC_GC Cuts at: 3574 3574 Size: 6127 NsiI A_TGCA'T Cuts at: 2265 2265 Size: 6127 NspI r_CATG'y Cuts at: 1132 1229 3485 5030 1132 Size: 97 2256 1545 2229 Fragments arranged by size: 2256 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6127 PciI A'CATG_T Cuts at: 5026 5026 Size: 6127 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6127 PfoI T'CCnGG_A Cuts at: 2157 2157 Size: 6127 PmeI GTTT'AAAC Cuts at: 3431 3431 Size: 6127 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4196 4228 5709 5741 4196 Size: 32 1481 32 4582 Fragments arranged by size: 4582 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6127 PsiI TTA'TAA Cuts at: 3697 3697 Size: 6127 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2748 2780 2748 Size: 32 6095 PstI C_TGCA'G Cuts at: 830 2246 3479 830 Size: 1416 1233 3478 Fragments arranged by size: 3478 1416 1233 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6127 SalI G'TCGA_C Cuts at: 1936 3469 1936 Size: 1533 4594 SbfI CC_TGCA'GG Cuts at: 2246 3479 2246 Size: 1233 4894 ScaI AGT'ACT Cuts at: 4375 6087 4375 Size: 1712 4415 SfcI C'TryA_G Cuts at: 826 1045 2242 3475 4610 5291 5482 826 Size: 219 1197 1233 1135 681 191 1471 Fragments arranged by size: 1471 1233 1197 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2246 2246 Size: 6127 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6127 SmaI CCC'GGG Cuts at: 1872 2049 3454 1872 Size: 177 1405 4545 Fragments arranged by size: 4545 1405 177 SmlI C'TyrA_G Cuts at: 820 1017 1943 4231 5132 5394 820 Size: 197 926 2288 901 262 1553 Fragments arranged by size: 2288 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2500 3918 493 Size: 2007 1418 2702 Fragments arranged by size: 2702 2007 1418 SpeI A'CTAG_T Cuts at: 152 152 Size: 6127 SphI G_CATG'C Cuts at: 1132 3485 1132 Size: 2353 3774 SrfI GCCC'GGGC Cuts at: 2049 2049 Size: 6127 SspI AAT'ATT Cuts at: 5 52 2822 4051 5 Size: 47 2770 1229 2081 Fragments arranged by size: 2770 2081 1229 47 StuI AGG'CCT Cuts at: 1517 2240 1517 Size: 723 5404 StyI C'CwwG_G Cuts at: 513 1065 2004 2190 2199 2250 3566 513 Size: 552 939 186 9 51 1316 3074 Fragments arranged by size: 3074 1316 939 552 186 51 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2158 4154 4171 4324 4509 4954 325 Size: 1255 578 1996 17 153 185 445 1498 Fragments arranged by size: 1996 1498 1255 578 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2106 3124 4373 Size: 274 80 33 51 1443 129 1018 1249 Cuts at: 4373 6085 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1249 1018 274 138 129 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3267 3299 6087 6119 3267 Size: 32 2788 32 3275 Fragments arranged by size: 3275 2788 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6127 XbaI T'CTAG_A Cuts at: 3463 3463 Size: 6127 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6127 XmaI C'CCGG_G Cuts at: 1870 2047 3452 1870 Size: 177 1405 4545 Fragments arranged by size: 4545 1405 177 XmnI GAAnn'nnTTC Cuts at: 4256 4256 Size: 6127 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3935 276 Size: 53 83 186 936 2401 2468 Fragments arranged by size: 2468 2401 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbeI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfoI SwaI XcmI