################################## # NO CUT ENZYMES ( 183 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BclI BcnI BglI BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HhaI Hin1I HinP1I HindIII HpaII HphI KasI KpnI KspI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Tsp45I Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AciI 97 CCGC CCGC(-3/-1) AfaI 190 GTAC GT^AC AflII 16 CTTAAG C^TTAAG AflIII 489 ACATGT A^C[GA][CT]GT Asp700I 487 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbsI 250 GAAGAC GAAGAC(2/6) BciVI 84 GTATCC GTATCC(6/5) BfrI 16 CTTAAG C^TTAAG BglII 168 AGATCT A^GATCT BpuAI 250 GAAGAC GAAGAC(2/6) BsiYI 379 CCTCCCTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 379 CCTCCCTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmI 276 GAATGC GAATGC(1/-1) BspLU11I 489 ACATGT A^CATGT BspMI 554 ACCTGC ACCTGC(4/8) BssKI 92 CCAGG ^CC[ACGT]GG BstNI 92 CCAGG CC^[AT]GG BstYI 168 AGATCT [GA]^GATC[CT] DdeI 193 CTTAG C^T[ACGT]AG DpnII 169 GATC ^GATC EarI 283 GAAGAG CTCTTC(1/4) EcoRII 92 CCAGG ^CC[AT]GG HaeIII 95 GGCC GG^CC HgaI 253 GACGC GACGC(5/10) HincII 294 GTTAAC GT[CT]^[GA]AC HindII 294 GTTAAC GT[CT]^[GA]AC HpaI 294 GTTAAC GTT^AAC Ksp632I 283 GAAGAG CTCTTC(1/4) MboI 169 GATC ^GATC MflI 168 AGATCT [GA]^GATC[CT] MslI 597 CATTGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 92 CCAGG CC^[AT]GG MwoI 445 GCAAGAACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeII 169 GATC ^GATC NlaIV 388 GGGACC GG[ACGT]^[ACGT]CC PleI 30 GAGTC GAGTC(4/5) PstI 506 CTGCAG CTGCA^G RsaI 190 GTAC GT^AC Sau3AI 169 GATC ^GATC ScaI 189 AGTACT AGT^ACT ScrFI 92 CCAGG CC^[ACGT]GG SfcI 506 CTGCAG C^T[GA][CT]AG SmlI 16 CTTAAG C^T[CT][GA]AG TfiI 375 GAATC G^A[AT]TC TspRI 590 CAGTG CA[GC]TG[ACGT][ACGT]^ XcmI 65 CCAAGCTACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 168 AGATCT [GA]^GATC[CT] XmnI 487 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AluI 68 AGCT AG^CT 504 AGCT AvaII 389 GGACC G^G[AT]CC 606 GGACC BbvI 106 GCAGC GCAGC(8/12) 505 GCTGC BsmFI 388 GGGAC GGGAC(10/14) 406 GGGAC BsrDI 102 CATTGC GCAATG(2/0) 208 GCAATG BstF5I 48 GGATG GGATG(2/0) 175 GGATG Cac8I 35 GCCTGC GC[ACGT]^[ACGT]GC 558 GCCAGC Cfr13I 389 GGACC G^G[ACGT]CC 606 GGACC FokI 48 GGATG GGATG(9/13) 175 GGATG HinfI 30 GAGTC G^A[ACGT]TC 375 GAATC MnlI 379 CCTC CCTC(7/6) 476 CCTC NspI 109 GCATGT [GA]CATG^[CT] 489 ACATGT Sau96I 389 GGACC G^G[ACGT]CC 606 GGACC SfaNI 197 GCATC GCATC(5/9) 562 GCATC SspI 264 AATATT AAT^ATT 301 AATATT TaqI 359 TCGA T^CGA 546 TCGA TseI 106 GCAGC G^C[AT]GC 505 GCTGC Tsp509I 369 AATT ^AATT 612 AATT TthHB8I 359 TCGA T^CGA 546 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 8 enzymes) # ######################################### BfaI Fnu4HI ItaI MaeI MboII MseI NlaIII Tru9I