Restriction map for
pFN21AE3978
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
Alw44I AlwI AlwNI Aor51HI ApaI
ApaLI AscI Asp718I AspI AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbsI BbvCI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI Bsp1407I
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstUI BstXI BstYI BstZ17I CelII
CfoI Cfr10I CpoI DraII DraIII
DrdI DsaI EaeI EagI EarI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FokI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI HpaII
KasI KpnI Ksp632I KspI MaeII
MamI MfeI MflI MluI MluNI
MroI MscI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccI 39 GTCTAC GT^[AC][GT]AC
AfaI 988 GTAC GT^AC
AhdI 244 GACTACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 204 CCTGAGG CC^T[ACGT]AGG
Asp700I 1105 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 244 GACTACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 5 CTCGGG C^[CT]CG[GA]G
BcgI 605 GCAGTTTTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsoBI 5 CTCGGG C^[CT]CG[GA]G
BspDI 368 ATCGAT AT^CGAT
BsrDI 346 GCAATG GCAATG(2/0)
BssKI 28 CCTGG ^CC[ACGT]GG
BstNI 28 CCTGG CC^[AT]GG
Bsu36I 204 CCTGAGG CC^T[ACGT]AGG
Cac8I 651 GCTTGC GC[ACGT]^[ACGT]GC
ClaI 368 ATCGAT AT^CGAT
DpnII 662 GATC ^GATC
Eam1105I 244 GACTACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 204 CCTGAGG CC^T[ACGT]AGG
EcoRII 28 CCTGG ^CC[AT]GG
HaeIII 178 GGCC GG^CC
MboI 662 GATC ^GATC
MslI 1058 CATCAGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 28 CCTGG CC^[AT]GG
NdeII 662 GATC ^GATC
PflMI 1138 CCACAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 988 GTAC GT^AC
Sau3AI 662 GATC ^GATC
ScrFI 28 CCTGG CC^[ACGT]GG
SfaNI 1123 GCATC GCATC(5/9)
Tsp45I 185 GTGAC ^GT[GC]AC
Van91I 1138 CCACAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1105 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AseI 112 ATTAAT AT^TAAT
973 ATTAAT
AsnI 112 ATTAAT AT^TAAT
973 ATTAAT
AspHI 785 GAGCAC G[AT]GC[AT]^C
1115 GAGCAC
BanI 200 GGTGCC G^G[CT][GA]CC
540 GGTGCC
BfaI 556 CTAG C^TAG
1120 CTAG
BmyI 785 GAGCAC G[AGT]GC[ACT]^C
1115 GAGCAC
BpmI 665 CTCCAG CTGGAG(16/14)
29 CTGGAG
BsiHKAI 785 GAGCAC G[AT]GC[AT]^C
1115 GAGCAC
BsmAI 3 GTCTC GTCTC(1/5)
1083 GTCTC
BsmFI 416 GGGAC GGGAC(10/14)
47 GTCCC
BsmI 321 GAATGC GAATGC(1/-1)
1148 GCATTC
Bsp1286I 785 GAGCAC G[AGT]GC[ACT]^C
1115 GAGCAC
HindIII 460 AAGCTT A^AGCTT
872 AAGCTT
MaeI 556 CTAG C^TAG
1120 CTAG
MspA1I 273 CAGCTG C[AC]G^C[GT]G
824 CAGCTG
MwoI 1108 GCCTTTCGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1148 GCATTCCACGC
PshBI 112 ATTAAT AT^TAAT
973 ATTAAT
PstI 603 CTGCAG CTGCA^G
827 CTGCAG
PvuII 273 CAGCTG CAG^CTG
824 CAGCTG
SfcI 603 CTGCAG C^T[GA][CT]AG
827 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AcsI AluI ApoI AvaII BbvI
BsgI BsiYI BslI BsrI Cfr13I
DdeI DraI Fnu4HI HinfI HphI
ItaI MaeIII MboII MnlI MseI
NlaIII NlaIV Sau96I TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I