(Circular) (Six-base) MAPSORT of: pFN21AE3978.seq Check: 7378 from: 1 to: 5881 pFN21AE3978 5881 bp With 182 enzymes: SgfI * March 28, 2012 11:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3691 278 Size: 53 83 186 936 2155 2468 Fragments arranged by size: 2468 2155 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2045 3224 1937 Size: 108 1179 4594 Fragments arranged by size: 4594 1179 108 Acc65I G'GTAC_C Cuts at: 3202 3202 Size: 5881 AclI AA'CG_TT Cuts at: 1563 4008 4381 1563 Size: 2445 373 3063 Fragments arranged by size: 3063 2445 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2110 2769 2879 3944 5328 5596 1632 Size: 478 659 110 1065 1384 268 1917 Fragments arranged by size: 1917 1384 1065 659 478 268 110 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5684 AflIII A'CryG_T Cuts at: 1203 4780 5757 1203 Size: 3577 977 1327 Fragments arranged by size: 3577 1327 977 AgeI A'CCGG_T Cuts at: 4712 4712 Size: 5881 AhdI GACnn_n'nnGTC Cuts at: 2254 4610 2254 Size: 2356 3525 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5881 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5881 AlwNI CAG_nnn'CTG Cuts at: 3624 5196 3624 Size: 1572 4309 ApaLI G'TGCA_C Cuts at: 3938 5094 3938 Size: 1156 4725 ApoI r'AATT_y Cuts at: 1493 2096 2306 2312 2408 2633 3190 3416 3568 Size: 603 210 6 96 225 557 226 152 Cuts at: 3568 5677 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 603 557 226 225 210 152 96 6 AseI AT'TA_AT Cuts at: 160 1032 2118 2979 4435 160 Size: 872 1086 861 1456 1606 Fragments arranged by size: 1606 1456 1086 872 861 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5881 AvaI C'yCGr_G Cuts at: 1870 1943 2010 3206 1870 Size: 73 67 1196 4545 Fragments arranged by size: 4545 1196 73 67 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2907 2940 2907 Size: 33 5848 BamHI G'GATC_C Cuts at: 3211 3578 3211 Size: 367 5514 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2205 2545 3202 4657 618 Size: 325 217 627 418 340 657 1455 1842 Fragments arranged by size: 1842 1455 657 627 418 340 325 217 BanII G_rGCy'C Cuts at: 1391 3200 1391 Size: 1809 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4910 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2599 2633 4072 4106 1903 Size: 34 662 34 1439 34 3678 Fragments arranged by size: 3678 1439 662 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3773 4983 3773 Size: 1210 4671 BclI T'GATC_A Cuts at: 1548 1548 Size: 5881 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4492 136 Size: 107 122 71 631 605 2820 1525 Fragments arranged by size: 2820 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5876 5876 Size: 5881 BlpI GC'TnA_GC Cuts at: 3298 3298 Size: 5881 Bme1580I G_kGCm'C Cuts at: 1790 3942 5098 1790 Size: 2152 1156 2573 Fragments arranged by size: 2573 2152 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5881 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2404 3612 4570 Size: 416 53 83 399 24 987 1208 958 Cuts at: 4570 442 Size: 1753 Fragments arranged by size: 1753 1208 987 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5881 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2055 2655 4541 1239 Size: 816 600 1886 2579 Fragments arranged by size: 2579 1886 816 600 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4006 4871 5169 4006 Size: 865 298 4718 Fragments arranged by size: 4718 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4544 882 Size: 617 3045 2219 Fragments arranged by size: 3045 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3672 493 Size: 711 2468 2702 Fragments arranged by size: 2702 2468 711 BsaBI GATnn'nnATC Cuts at: 5875 5875 Size: 5881 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3688 4070 4705 275 Size: 53 83 186 936 2155 382 635 1451 Fragments arranged by size: 2155 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4314 4712 4986 5133 5544 4314 Size: 398 274 147 411 4651 Fragments arranged by size: 4651 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5656 Fragments arranged by size: 5656 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5881 BseYI C'CCAG_C Cuts at: 5084 5580 5084 Size: 496 5385 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2964 3007 3117 1163 Size: 573 1228 43 110 3927 Fragments arranged by size: 3927 1228 573 110 43 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3331 4092 5120 1540 Size: 275 186 1330 761 1028 2301 Fragments arranged by size: 2301 1330 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2794 3124 3942 4027 5098 2794 Size: 330 818 85 1071 3577 Fragments arranged by size: 3577 1071 818 330 85 BsmI GAATG_Cn' Cuts at: 2332 3153 3392 3485 2332 Size: 821 239 93 4728 Fragments arranged by size: 4728 821 239 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2794 3124 3200 3942 4027 5098 1391 Size: 399 1004 330 76 742 85 1071 2174 Fragments arranged by size: 2174 1071 1004 742 399 330 85 76 BspEI T'CCGG_A Cuts at: 5544 5544 Size: 5881 BspHI T'CATG_A Cuts at: 3770 5500 3770 Size: 1730 4151 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3236 844 Size: 2392 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3768 1394 Size: 429 1945 3507 Fragments arranged by size: 3507 1945 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2358 4376 4550 65 Size: 1183 1110 2018 174 1396 Fragments arranged by size: 2018 1396 1183 1110 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4525 4712 1700 Size: 110 2715 187 2869 Fragments arranged by size: 2869 2715 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5881 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5881 BssSI C'ACGA_G Cuts at: 3941 4953 3941 Size: 1012 4869 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5881 BstBI TT'CG_AA Cuts at: 4685 4685 Size: 5881 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5881 BstYI r'GATC_y Cuts at: 1972 3211 3578 3963 3980 5432 5518 5530 5876 Size: 1239 367 385 17 1452 86 12 346 Cuts at: 5876 1972 Size: 1977 Fragments arranged by size: 1977 1452 1239 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2210 2210 Size: 5881 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5329 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5142 Fragments arranged by size: 5142 683 56 BtsI GCAGTG_nn' Cuts at: 2995 3397 4191 4211 2995 Size: 402 794 20 4665 Fragments arranged by size: 4665 794 402 20 ClaI AT'CG_AT Cuts at: 1554 2374 1554 Size: 820 5061 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5846 DraI TTT'AAA Cuts at: 2404 2638 2758 3185 2404 Size: 234 120 427 5100 Fragments arranged by size: 5100 427 234 120 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4888 809 Size: 507 3572 1802 Fragments arranged by size: 3572 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3328 4217 5846 Size: 54 1425 50 275 18 1498 889 1629 Cuts at: 5846 8 Size: 43 Fragments arranged by size: 1629 1498 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3328 1537 Size: 275 1516 4090 Fragments arranged by size: 4090 1516 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3811 1353 Size: 2458 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4468 4842 4988 387 Size: 1064 138 2879 374 146 1280 Fragments arranged by size: 2879 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2055 2110 2655 2769 2879 3944 4541 Size: 393 423 55 545 114 110 1065 597 Cuts at: 4541 5328 5596 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1065 787 597 545 423 393 268 114 110 55 EcoRI G'AATT_C Cuts at: 3190 3568 3190 Size: 378 5503 FspI TGC'GCA Cuts at: 4387 4387 Size: 5881 HaeII r_GCGC'y Cuts at: 1678 5028 1678 Size: 3350 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2240 2272 3073 3105 4522 Size: 32 135 32 668 32 801 32 1417 Cuts at: 4522 4554 4596 4628 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1417 801 668 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3225 3471 669 Size: 988 281 1287 246 3079 Fragments arranged by size: 3079 1287 988 281 246 HindIII A'AGCT_T Cuts at: 748 2465 2877 748 Size: 1717 412 3752 Fragments arranged by size: 3752 1717 412 HpaI GTT'AAC Cuts at: 3471 3471 Size: 5881 KpnI G_GTAC'C Cuts at: 3206 3206 Size: 5881 MfeI C'AATT_G Cuts at: 3480 3480 Size: 5881 MluI A'CGCG_T Cuts at: 5757 5757 Size: 5881 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4995 5179 1305 Size: 3690 184 2007 Fragments arranged by size: 3690 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4059 Fragments arranged by size: 4059 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3067 3839 4198 4357 518 Size: 648 1901 772 359 159 2042 Fragments arranged by size: 2042 1901 772 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2280 2831 3297 3974 5122 5367 1606 Size: 674 551 466 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 674 551 466 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5329 NdeI CA'TA_TG Cuts at: 387 387 Size: 5881 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5881 NotI GC'GGCC_GC Cuts at: 3328 3328 Size: 5881 NspI r_CATG'y Cuts at: 1132 1229 3239 4784 1132 Size: 97 2010 1545 2229 Fragments arranged by size: 2229 2010 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5881 PciI A'CATG_T Cuts at: 4780 4780 Size: 5881 PflMI CCAn_nnn'nTGG Cuts at: 1108 3149 1108 Size: 2041 3840 PmeI GTTT'AAAC Cuts at: 3185 3185 Size: 5881 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3950 3982 5463 5495 3950 Size: 32 1481 32 4336 Fragments arranged by size: 4336 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5881 PsiI TTA'TAA Cuts at: 3451 3451 Size: 5881 PstI C_TGCA'G Cuts at: 830 2612 2836 3233 830 Size: 1782 224 397 3478 Fragments arranged by size: 3478 1782 397 224 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5881 PvuII CAG'CTG Cuts at: 2280 2831 2280 Size: 551 5330 SalI G'TCGA_C Cuts at: 1936 3223 1936 Size: 1287 4594 SbfI CC_TGCA'GG Cuts at: 3233 3233 Size: 5881 ScaI AGT'ACT Cuts at: 4129 5841 4129 Size: 1712 4169 SfcI C'TryA_G Cuts at: 826 1045 2608 2832 3229 4364 5045 5236 826 Size: 219 1563 224 397 1135 681 191 1471 Fragments arranged by size: 1563 1471 1135 681 397 224 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5881 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5881 SmaI CCC'GGG Cuts at: 1872 3208 1872 Size: 1336 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3985 4886 5148 820 Size: 197 926 2042 901 262 1553 Fragments arranged by size: 2042 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3672 493 Size: 3179 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5881 SphI G_CATG'C Cuts at: 1132 3239 1132 Size: 2107 3774 SspI AAT'ATT Cuts at: 5 52 3805 5 Size: 47 3753 2081 Fragments arranged by size: 3753 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5881 StyI C'CwwG_G Cuts at: 513 1065 3320 513 Size: 552 2255 3074 Fragments arranged by size: 3074 2255 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2138 2997 3908 3925 4078 4263 4708 Size: 1255 558 859 911 17 153 185 445 Cuts at: 4708 325 Size: 1498 Fragments arranged by size: 1498 1255 911 859 558 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2992 4127 5839 Size: 274 80 33 51 1443 1015 1135 1712 Cuts at: 5839 96 Size: 138 Fragments arranged by size: 1712 1443 1135 1015 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5841 5873 5841 Size: 32 5849 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5881 XbaI T'CTAG_A Cuts at: 3217 3217 Size: 5881 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5881 XmaI C'CCGG_G Cuts at: 1870 3206 1870 Size: 1336 4545 XmnI GAAnn'nnTTC Cuts at: 3114 4010 3114 Size: 896 4985 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3689 276 Size: 53 83 186 936 2155 2468 Fragments arranged by size: 2468 2155 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI