################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## Acc65I AclI AflIII AgeI Aor51HI AscI AseI AsnI Asp718I AviII BbrPI BsaAI BsaBI BsiWI BsmBI BsmI Bsp1407I BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I CpoI DraI Eco47III EcoRV FseI FspI HindIII HpaI KpnI MamI MfeI MluI MunI NaeI NdeI NgoMIV NheI NruI NspI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SbfI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 1507 GACGTC GACGT^C AccI 3432 GTCGAC GT^[AC][GT]AC AccIII 1294 TCCGGA T^CCGGA AflII 1108 CTTAAG C^TTAAG Alw44I 775 GTGCAC G^TGCAC AocI 2749 CCTAAGG CC^T[ACGT]AGG ApaI 2159 GGGCCC GGGCC^C ApaLI 775 GTGCAC G^TGCAC Asp700I 2815 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 2001 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 2155 CCTAGG C^CTAGG BbeI 1795 GGCGCC GGCGC^C BbvCI 1781 CCTCAGC CCTCAGC(-5/-2) BcgI 3019 GCAGCATTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1015 GTATCC GTATCC(6/5) BclI 2112 TGATCA T^GATCA BfrI 1108 CTTAAG C^TTAAG BglII 880 AGATCT A^GATCT BlnI 2155 CCTAGG C^CTAGG BseAI 1294 TCCGGA T^CCGGA BspDI 322 ATCGAT AT^CGAT BspEI 1294 TCCGGA T^CCGGA BstAPI 3648 GCACTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 3594 GGTCACC G^GT[ACGT]ACC BstPI 3594 GGTCACC G^GT[ACGT]ACC Bsu36I 2749 CCTAAGG CC^T[ACGT]AGG ClaI 322 ATCGAT AT^CGAT DraIII 47 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 2749 CCTAAGG CC^T[ACGT]AGG EcoO65I 3594 GGTCACC G^GT[ACGT]ACC EcoRI 3559 GAATTC G^AATTC FbaI 2112 TGATCA T^GATCA HgaI 1834 GACGC GACGC(5/10) HincII 3432 GTCGAC GT[CT]^[GA]AC HindII 3432 GTCGAC GT[CT]^[GA]AC KasI 1795 GGCGCC G^GCGCC MroI 1294 TCCGGA T^CCGGA MslI 3311 CATCTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 1795 GGCGCC GG^CGCC NotI 1825 GCGGCCGC GC^GGCCGC PaeR7I 40 CTCGAG C^TCGAG PflFI 2001 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 3435 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 3109 GAGCTC GAGCT^C SalI 3432 GTCGAC G^TCGAC SapI 2522 GCTCTTC GCTCTTC(1/4) SexAI 3620 ACCTGGT A^CC[AT]GGT SfoI 1795 GGCGCC GGC^GCC Tth111I 2001 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 40 CTCGAG C^TCGAG XmnI 2815 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcsI 982 AAATTT [GA]^AATT[CT] 3559 GAATTC AcyI 1507 GACGTC G[GA]^CG[CT]C 1795 GGCGCC AhdI 37 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2038 GACCTGGAGTC ApoI 982 AAATTT [GA]^AATT[CT] 3559 GAATTC AspEI 37 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2038 GACCTGGAGTC BalI 2534 TGGCCA TGG^CCA 3542 TGGCCA BamHI 203 GGATCC G^GATCC 2330 GGATCC BlpI 1113 GCTAAGC GC^T[ACGT]AGC 1962 GCTAAGC Bpu1102I 1113 GCTAAGC GC^T[ACGT]AGC 1962 GCTAAGC BsaHI 1507 GACGTC G[GA]^CG[CT]C 1795 GGCGCC BsaWI 200 ACCGGA [AT]^CCGG[AT] 1294 TCCGGA CelII 1113 GCTAAGC GC^T[ACGT]AGC 1962 GCTAAGC DrdI 2001 GACAGAGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2316 GACCCTCCTGTC Eam1105I 37 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2038 GACCTGGAGTC EcoT22I 74 ATGCAT ATGCA^T 2654 ATGCAT Hin1I 1507 GACGTC G[GA]^CG[CT]C 1795 GGCGCC MluNI 2534 TGGCCA TGG^CCA 3542 TGGCCA MscI 2534 TGGCCA TGG^CCA 3542 TGGCCA NcoI 602 CCATGG C^CATGG 3065 CCATGG NsiI 74 ATGCAT ATGCA^T 2654 ATGCAT PflMI 1066 CCAGACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3462 CCACCAGATGG ScaI 1484 AGTACT AGT^ACT 3629 AGTACT SmlI 40 CTCGAG C^T[CT][GA]AG 1108 CTTAAG TfiI 343 GATTC G^A[AT]TC 3070 GAATC Van91I 1066 CCAGACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3462 CCACCAGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII