(Circular) (Six-base) MAPSORT of: pFN21AB4854.seq Check: 3070 from: 1 to: 8383 pFN21AB4854 8383 bp With 182 enzymes: SgfI * January 30, 2014 17:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2611 4356 4438 2611 Size: 1745 82 6556 Fragments arranged by size: 6556 1745 82 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3516 6193 278 Size: 53 83 186 936 1980 2677 2468 Fragments arranged by size: 2677 2468 1980 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5438 5726 1937 Size: 3501 288 4594 Fragments arranged by size: 4594 3501 288 Acc65I G'GTAC_C Cuts at: 5704 5704 Size: 8383 AclI AA'CG_TT Cuts at: 1563 6510 6883 1563 Size: 4947 373 3063 Fragments arranged by size: 4947 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2146 2567 2777 4148 4716 5040 6446 7830 Size: 514 421 210 1371 568 324 1406 1384 Cuts at: 7830 8098 1632 Size: 268 1917 Fragments arranged by size: 1917 1406 1384 1371 568 514 421 324 268 210 AflII C'TTAA_G Cuts at: 820 1017 3113 820 Size: 197 2096 6090 Fragments arranged by size: 6090 2096 197 AflIII A'CryG_T Cuts at: 1203 7282 8259 1203 Size: 6079 977 1327 Fragments arranged by size: 6079 1327 977 AgeI A'CCGG_T Cuts at: 7214 7214 Size: 8383 AhdI GACnn_n'nnGTC Cuts at: 2047 4048 7112 2047 Size: 2001 3064 3318 Fragments arranged by size: 3318 3064 2001 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8383 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3941 4591 1184 Size: 2757 650 4976 Fragments arranged by size: 4976 2757 650 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2625 2657 4211 4243 2625 Size: 32 1554 32 6765 Fragments arranged by size: 6765 1554 32 32 AlwNI CAG_nnn'CTG Cuts at: 2408 3891 4126 4890 5650 6126 7698 2408 Size: 1483 235 764 760 476 1572 3093 Fragments arranged by size: 3093 1572 1483 764 760 476 235 ApaI G_GGCC'C Cuts at: 4168 4168 Size: 8383 ApaLI G'TGCA_C Cuts at: 2780 6440 7596 2780 Size: 3660 1156 3567 Fragments arranged by size: 3660 3567 1156 ApoI r'AATT_y Cuts at: 1493 2987 5564 5692 5918 6070 8179 1493 Size: 1494 2577 128 226 152 2109 1697 Fragments arranged by size: 2577 2109 1697 1494 226 152 128 AseI AT'TA_AT Cuts at: 160 1032 6937 160 Size: 872 5905 1606 Fragments arranged by size: 5905 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8383 AvaI C'yCGr_G Cuts at: 1870 1943 2045 2547 3818 5708 1870 Size: 73 102 502 1271 1890 4545 Fragments arranged by size: 4545 1890 1271 502 102 73 AvrII C'CTAG_G Cuts at: 4160 4160 Size: 8383 BamHI G'GATC_C Cuts at: 2208 4335 5713 6080 2208 Size: 2127 1378 367 4511 Fragments arranged by size: 4511 2127 1378 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2460 3223 3800 5704 7159 Size: 325 217 627 673 763 577 1904 1455 Cuts at: 7159 618 Size: 1842 Fragments arranged by size: 1904 1842 1455 763 673 627 577 325 217 BanII G_rGCy'C Cuts at: 1391 2172 2359 2504 3828 3876 4168 5118 5702 Size: 781 187 145 1324 48 292 950 584 Cuts at: 5702 1391 Size: 4072 Fragments arranged by size: 4072 1324 950 781 584 292 187 145 48 BbeI G_GCGC'C Cuts at: 3804 3804 Size: 8383 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2757 4488 4903 5178 928 Size: 971 858 1731 415 275 4133 Fragments arranged by size: 4133 1731 971 858 415 275 BbvCI CC'TCA_GC Cuts at: 3787 3787 Size: 8383 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5013 5047 6574 6608 1903 Size: 34 3076 34 1527 34 3678 Fragments arranged by size: 3678 3076 1527 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3031 6275 7485 3031 Size: 3244 1210 3929 Fragments arranged by size: 3929 3244 1210 BclI T'GATC_A Cuts at: 1548 4117 1548 Size: 2569 5814 BfrBI ATG'CAT Cuts at: 2081 4661 2081 Size: 2580 5803 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2612 3617 3769 Size: 107 122 71 631 605 940 1005 152 Cuts at: 3769 6994 136 Size: 3225 1525 Fragments arranged by size: 3225 1525 1005 940 631 605 152 122 107 71 BglII A'GATC_T Cuts at: 2885 8378 2885 Size: 5493 2890 BlpI GC'TnA_GC Cuts at: 3119 3968 5800 3119 Size: 849 1832 5702 Fragments arranged by size: 5702 1832 849 Bme1580I G_kGCm'C Cuts at: 1790 2463 2784 3708 4168 6444 7600 1790 Size: 673 321 924 460 2276 1156 2573 Fragments arranged by size: 2573 2276 1156 924 673 460 321 BmgBI CAC'GTC Cuts at: 1318 5488 1318 Size: 4170 4213 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5536 6114 7072 Size: 416 53 83 399 24 4119 578 958 Cuts at: 7072 442 Size: 1753 Fragments arranged by size: 4119 1753 958 578 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8383 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3183 3313 3343 3802 3946 3183 Size: 130 30 459 144 7620 Fragments arranged by size: 7620 459 144 130 30 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2015 2910 3262 3313 3349 4067 4238 4851 Size: 776 895 352 51 36 718 171 613 Cuts at: 4851 7043 1239 Size: 2192 2579 Fragments arranged by size: 2579 2192 895 776 718 613 352 171 51 36 Bpu10I CC'TnA_GC Cuts at: 2751 3787 2751 Size: 1036 7347 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6508 7373 7671 6508 Size: 865 298 7220 Fragments arranged by size: 7220 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3308 4302 4782 4869 5403 7046 882 Size: 617 1809 994 480 87 534 1643 2219 Fragments arranged by size: 2219 1809 1643 994 617 534 480 87 BsaAI yAC'GTr Cuts at: 493 1204 6174 493 Size: 711 4970 2702 Fragments arranged by size: 4970 2702 711 BsaBI GATnn'nnATC Cuts at: 8377 8377 Size: 8383 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3513 3801 6190 6572 Size: 53 83 186 936 1980 288 2389 382 Cuts at: 6572 7207 275 Size: 635 1451 Fragments arranged by size: 2389 1980 1451 936 635 382 288 186 83 53 BsaWI w'CCGG_w Cuts at: 2205 3299 6816 7214 7488 7635 8046 2205 Size: 1094 3517 398 274 147 411 2542 Fragments arranged by size: 3517 2542 1094 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2514 2544 4211 4241 1178 Size: 30 165 30 1111 30 1667 30 5320 Fragments arranged by size: 5320 1667 1111 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2891 3149 3265 3605 3836 3922 4018 4021 Size: 1705 258 116 340 231 86 96 3 Cuts at: 4021 4076 4096 4235 1186 Size: 55 20 139 5334 Fragments arranged by size: 5334 1705 340 258 231 139 116 96 86 55 20 3 BseYI C'CCAG_C Cuts at: 2456 2675 3551 3844 4409 7586 8082 2456 Size: 219 876 293 565 3177 496 2757 Fragments arranged by size: 3177 2757 876 565 496 293 219 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2438 2770 5495 5591 5636 1163 Size: 573 702 332 2725 96 45 3910 Fragments arranged by size: 3910 2725 702 573 332 96 45 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2700 3663 3834 4637 4847 5833 Size: 275 186 699 963 171 803 210 986 Cuts at: 5833 6594 7622 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 986 963 803 761 699 275 210 186 171 BsiHKAI G_wGCw'C Cuts at: 2784 3581 5118 5345 5625 6444 6529 7600 2784 Size: 797 1537 227 280 819 85 1071 3567 Fragments arranged by size: 3567 1537 1071 819 797 280 227 85 BsmI GAATG_Cn' Cuts at: 5894 5987 5894 Size: 93 8290 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2172 2359 2463 2504 2784 3581 3708 Size: 399 382 187 104 41 280 797 127 Cuts at: 3708 3828 3876 4168 5118 5345 5625 5702 6444 Size: 120 48 292 950 227 280 77 742 Cuts at: 6444 6529 7600 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 950 797 742 399 382 292 280 280 227 187 127 120 104 85 77 48 41 BspEI T'CCGG_A Cuts at: 3299 8046 3299 Size: 4747 3636 BspHI T'CATG_A Cuts at: 6272 8002 6272 Size: 1730 6653 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2611 3950 4356 4438 5738 844 Size: 1767 1339 406 82 1300 3489 Fragments arranged by size: 3489 1767 1339 1300 406 82 BsrBI CCG'CTC Cuts at: 1394 1823 6270 1394 Size: 429 4447 3507 Fragments arranged by size: 4447 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2572 4989 5061 6878 7052 65 Size: 1183 1324 2417 72 1817 174 1396 Fragments arranged by size: 2417 1817 1396 1324 1183 174 72 BsrFI r'CCGG_y Cuts at: 1700 1810 3382 3813 5596 7027 7214 1700 Size: 110 1572 431 1783 1431 187 2869 Fragments arranged by size: 2869 1783 1572 1431 431 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8383 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8383 BssSI C'ACGA_G Cuts at: 6443 7455 6443 Size: 1012 7371 BstAPI GCAn_nnn'nTGC Cuts at: 794 5659 794 Size: 4865 3518 BstBI TT'CG_AA Cuts at: 7187 7187 Size: 8383 BstEII G'GTnAC_C Cuts at: 1184 5599 1184 Size: 4415 3968 BstXI CCAn_nnnn'nTGG Cuts at: 3524 4216 5322 5392 3524 Size: 692 1106 70 6515 Fragments arranged by size: 6515 1106 692 70 BstYI r'GATC_y Cuts at: 1972 2208 2261 2885 3302 4335 5581 5713 6080 Size: 236 53 624 417 1033 1246 132 367 Cuts at: 6080 6465 6482 7934 8020 8032 8378 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1246 1033 624 417 385 367 346 236 132 86 53 17 12 Bsu36I CC'TnA_GG Cuts at: 4755 4755 Size: 8383 BtgI C'CryG_G Cuts at: 513 1065 2004 2607 3192 3469 3657 3663 3669 Size: 552 939 603 585 277 188 6 6 Cuts at: 3669 3717 3750 3828 3834 4089 4859 5070 5321 Size: 48 33 78 6 255 770 211 251 Cuts at: 5321 5391 513 Size: 70 3505 Fragments arranged by size: 3505 939 770 603 585 552 277 255 251 211 188 78 70 48 33 6 6 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4189 487 Size: 683 56 2963 4681 Fragments arranged by size: 4681 2963 683 56 BtsI GCAGTG_nn' Cuts at: 2782 3130 3708 4159 4573 4936 5899 6693 6713 Size: 348 578 451 414 363 963 794 20 Cuts at: 6713 2782 Size: 4452 Fragments arranged by size: 4452 963 794 578 451 414 363 348 20 ClaI AT'CG_AT Cuts at: 1554 2328 1554 Size: 774 7609 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8348 DraI TTT'AAA Cuts at: 5687 5687 Size: 8383 DraIII CAC_nnn'GTG Cuts at: 2057 2057 Size: 8383 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4012 4327 7390 809 Size: 507 2696 315 3063 1802 Fragments arranged by size: 3063 2696 1802 507 315 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2476 2613 2722 Size: 54 1425 50 275 18 646 137 109 Cuts at: 2722 3660 3666 3831 4249 4539 4844 4879 5478 Size: 938 6 165 418 290 305 35 599 Cuts at: 5478 5535 5547 5830 6719 8348 8 Size: 57 12 283 889 1629 43 Fragments arranged by size: 1629 1425 938 889 646 599 418 305 290 283 275 165 137 109 57 54 50 43 35 18 12 6 EagI C'GGCC_G Cuts at: 1537 1812 3660 3831 4844 5830 1537 Size: 275 1848 171 1013 986 4090 Fragments arranged by size: 4090 1848 1013 986 275 171 EarI CTCTTCn'nnn_ Cuts at: 1353 2165 3126 4534 4744 6313 1353 Size: 812 961 1408 210 1569 3423 Fragments arranged by size: 3423 1569 1408 961 812 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4800 5239 6970 7344 7490 387 Size: 1064 138 3211 439 1731 374 146 1280 Fragments arranged by size: 3211 1731 1280 1064 439 374 146 138 EcoICRI GAG'CTC Cuts at: 5116 5116 Size: 8383 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2015 2146 2567 2777 2910 3262 3313 Size: 393 383 131 421 210 133 352 51 Cuts at: 3313 3349 4067 4148 4238 4716 4851 5040 6446 Size: 36 718 81 90 478 135 189 1406 Cuts at: 6446 7043 7830 8098 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1406 787 718 597 478 421 393 383 352 268 210 189 135 133 131 90 81 51 36 EcoNI CCTnn'n_nnAGG Cuts at: 2030 4352 5549 2030 Size: 2322 1197 4864 Fragments arranged by size: 4864 2322 1197 EcoO109I rG'GnC_Cy Cuts at: 2041 2511 2734 3619 3783 3888 4164 4165 5066 Size: 470 223 885 164 105 276 1 901 Cuts at: 5066 5153 5647 2041 Size: 87 494 4777 Fragments arranged by size: 4777 901 885 494 470 276 223 164 105 87 1 EcoRI G'AATT_C Cuts at: 5564 5692 6070 5564 Size: 128 378 7877 Fragments arranged by size: 7877 378 128 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3128 3128 Size: 8383 FspI TGC'GCA Cuts at: 6889 6889 Size: 8383 HaeII r_GCGC'y Cuts at: 1678 3192 3804 3955 4377 5427 5673 7530 1678 Size: 1514 612 151 422 1050 246 1857 2531 Fragments arranged by size: 2531 1857 1514 1050 612 422 246 151 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2033 2065 2183 2215 2870 Size: 32 135 32 461 32 118 32 655 Cuts at: 2870 2902 3995 4001 4027 4033 4034 4066 4280 Size: 32 1093 6 26 6 1 32 214 Cuts at: 4280 4282 4312 4314 7024 7056 7098 7130 1373 Size: 2 30 2 2710 32 42 32 2626 Fragments arranged by size: 2710 2626 1093 655 461 214 135 118 42 32 32 32 32 32 32 32 32 30 26 6 6 2 2 1 HincII GTy'rAC Cuts at: 669 1657 1938 5439 5727 5973 669 Size: 988 281 3501 288 246 3079 Fragments arranged by size: 3501 3079 988 288 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8383 HpaI GTT'AAC Cuts at: 5973 5973 Size: 8383 KasI G'GCGC_C Cuts at: 3800 3800 Size: 8383 KpnI G_GTAC'C Cuts at: 5708 5708 Size: 8383 MfeI C'AATT_G Cuts at: 5982 5982 Size: 8383 MluI A'CGCG_T Cuts at: 8259 8259 Size: 8383 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2226 2263 4032 4065 4899 5439 7497 7681 Size: 921 37 1769 33 834 540 2058 184 Cuts at: 7681 1305 Size: 2007 Fragments arranged by size: 2058 2007 1769 921 834 540 184 37 33 MscI TGG'CCA Cuts at: 10 64 1489 1832 4541 5549 10 Size: 54 1425 343 2709 1008 2844 Fragments arranged by size: 2844 2709 1425 1008 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5320 6341 6700 6859 518 Size: 648 4154 1021 359 159 2042 Fragments arranged by size: 4154 2042 1021 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2408 2631 2904 3261 3406 3659 3719 3752 Size: 802 223 273 357 145 253 60 33 Cuts at: 3752 3830 3836 4139 4771 5799 6476 7624 7869 Size: 78 6 303 632 1028 677 1148 245 Cuts at: 7869 1606 Size: 2120 Fragments arranged by size: 2120 1148 1028 802 677 632 357 303 273 253 245 223 145 78 60 33 6 NarI GG'CG_CC Cuts at: 3801 3801 Size: 8383 NcoI C'CATG_G Cuts at: 513 1065 2004 2607 5070 513 Size: 552 939 603 2463 3826 Fragments arranged by size: 3826 2463 939 603 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8383 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8383 NotI GC'GGCC_GC Cuts at: 3831 5830 3831 Size: 1999 6384 NsiI A_TGCA'T Cuts at: 2083 4663 2083 Size: 2580 5803 NspI r_CATG'y Cuts at: 1132 1229 5741 7286 1132 Size: 97 4512 1545 2229 Fragments arranged by size: 4512 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3623 4510 1348 Size: 2275 887 5221 Fragments arranged by size: 5221 2275 887 PciI A'CATG_T Cuts at: 7282 7282 Size: 8383 PflMI CCAn_nnn'nTGG Cuts at: 1108 3077 5473 1108 Size: 1969 2396 4018 Fragments arranged by size: 4018 2396 1969 PfoI T'CCnGG_A Cuts at: 2031 2922 3516 4109 4331 2031 Size: 891 594 593 222 6083 Fragments arranged by size: 6083 891 594 593 222 PmeI GTTT'AAAC Cuts at: 5687 5687 Size: 8383 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2354 2386 4211 4243 6452 6484 7965 7997 2354 Size: 32 1825 32 2209 32 1481 32 2740 Fragments arranged by size: 2740 2209 1825 1481 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2041 2511 3783 2041 Size: 470 1272 6641 Fragments arranged by size: 6641 1272 470 PshAI GACnn'nnGTC Cuts at: 1613 5444 1613 Size: 3831 4552 PsiI TTA'TAA Cuts at: 5953 5953 Size: 8383 PspOMI G'GGCC_C Cuts at: 4164 4164 Size: 8383 PspXI vC'TCGA_Gb Cuts at: 2045 2045 Size: 8383 PstI C_TGCA'G Cuts at: 830 3215 4435 4776 5735 830 Size: 2385 1220 341 959 3478 Fragments arranged by size: 3478 2385 1220 959 341 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8383 PvuII CAG'CTG Cuts at: 2408 2904 3406 4771 2408 Size: 496 502 1365 6020 Fragments arranged by size: 6020 1365 502 496 SacI G_AGCT'C Cuts at: 5118 5118 Size: 8383 SacII CC_GC'GG Cuts at: 3660 3720 3753 3831 3837 3660 Size: 60 33 78 6 8206 Fragments arranged by size: 8206 78 60 33 6 SalI G'TCGA_C Cuts at: 1936 5437 5725 1936 Size: 3501 288 4594 Fragments arranged by size: 4594 3501 288 SapI GCTCTTCn'nnn_ Cuts at: 4534 4534 Size: 8383 SbfI CC_TGCA'GG Cuts at: 5735 5735 Size: 8383 ScaI AGT'ACT Cuts at: 3491 5636 6631 8343 3491 Size: 2145 995 1712 3531 Fragments arranged by size: 3531 2145 1712 995 SexAI A'CCwGG_T Cuts at: 5625 5625 Size: 8383 SfcI C'TryA_G Cuts at: 826 1045 2380 3211 4170 4431 4489 4772 5731 Size: 219 1335 831 959 261 58 283 959 Cuts at: 5731 6866 7547 7738 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1335 1135 959 959 831 681 283 261 219 191 58 SfoI GGC'GCC Cuts at: 3802 3802 Size: 8383 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8383 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8383 SmaI CCC'GGG Cuts at: 1872 5710 1872 Size: 3838 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2045 3113 6487 7388 7650 820 Size: 197 926 102 1068 3374 901 262 1553 Fragments arranged by size: 3374 1553 1068 926 901 262 197 102 SnaBI TAC'GTA Cuts at: 493 6174 493 Size: 5681 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8383 SphI G_CATG'C Cuts at: 1132 5741 1132 Size: 4609 3774 SspI AAT'ATT Cuts at: 5 52 6307 5 Size: 47 6255 2081 Fragments arranged by size: 6255 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8383 StyI C'CwwG_G Cuts at: 513 1065 2004 2607 2835 3602 3990 4160 4209 Size: 552 939 603 228 767 388 170 49 Cuts at: 4209 4386 5070 5550 5822 513 Size: 177 684 480 272 3074 Fragments arranged by size: 3074 939 767 684 603 552 480 388 272 228 177 170 49 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2478 3332 4623 6410 6427 6580 6765 Size: 1255 898 854 1291 1787 17 153 185 Cuts at: 6765 7210 325 Size: 445 1498 Fragments arranged by size: 1787 1498 1291 1255 898 854 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3489 5217 5634 Size: 274 80 33 51 1443 1512 1728 417 Cuts at: 5634 6629 8341 96 Size: 995 1712 138 Fragments arranged by size: 1728 1712 1512 1443 995 417 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4183 4215 5448 5480 8343 8375 4183 Size: 32 1233 32 2863 32 4191 Fragments arranged by size: 4191 2863 1233 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4009 1316 Size: 2693 5690 XbaI T'CTAG_A Cuts at: 5719 5719 Size: 8383 XhoI C'TCGA_G Cuts at: 1943 2045 1943 Size: 102 8281 XmaI C'CCGG_G Cuts at: 1870 5708 1870 Size: 3838 4545 XmnI GAAnn'nnTTC Cuts at: 4824 6512 4824 Size: 1688 6695 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3514 6191 276 Size: 53 83 186 936 1980 2677 2468 Fragments arranged by size: 2677 2468 1980 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BsiWI BsmBI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PsrI RsrII SanDI SfiI SrfI SwaI XcmI