(Linear) (Six-base) MAPSORT of: KIBB4854.seq Check: 1083 from: 1 to: 3678 KIBB4854.orf With 182 enzymes: SgfI * January 29, 2014 11:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 606 2351 2433 3678 Size: 606 1745 82 1245 Fragments arranged by size: 1745 1245 606 82 AatII G_ACGT'C Cuts at: 0 1511 3678 Size: 1511 2167 AccI GT'mk_AC Cuts at: 0 3433 3678 Size: 3433 245 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 141 562 772 2143 2711 3035 3678 Size: 141 421 210 1371 568 324 643 Fragments arranged by size: 1371 643 568 421 324 210 141 AflII C'TTAA_G Cuts at: 0 1108 3678 Size: 1108 2570 AhdI GACnn_n'nnGTC Cuts at: 0 42 2043 3678 Size: 42 2001 1635 Fragments arranged by size: 2001 1635 42 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1936 2586 3678 Size: 1936 650 1092 Fragments arranged by size: 1936 1092 650 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 620 652 2206 2238 3678 Size: 620 32 1554 32 1440 Fragments arranged by size: 1554 1440 620 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 403 1886 2121 2885 3645 3678 Size: 403 1483 235 764 760 33 Fragments arranged by size: 1483 764 760 403 235 33 ApaI G_GGCC'C Cuts at: 0 2163 3678 Size: 2163 1515 ApaLI G'TGCA_C Cuts at: 0 775 3678 Size: 775 2903 ApoI r'AATT_y Cuts at: 0 982 3559 3678 Size: 982 2577 119 Fragments arranged by size: 2577 982 119 AvaI C'yCGr_G Cuts at: 0 40 542 1813 3678 Size: 40 502 1271 1865 Fragments arranged by size: 1865 1271 502 40 AvrII C'CTAG_G Cuts at: 0 2155 3678 Size: 2155 1523 BamHI G'GATC_C Cuts at: 0 203 2330 3678 Size: 203 2127 1348 Fragments arranged by size: 2127 1348 203 BanI G'GyrC_C Cuts at: 0 455 1218 1795 3678 Size: 455 763 577 1883 Fragments arranged by size: 1883 763 577 455 BanII G_rGCy'C Cuts at: 0 167 354 499 1823 1871 2163 3113 3678 Size: 167 187 145 1324 48 292 950 565 Fragments arranged by size: 1324 950 565 292 187 167 145 48 BbeI G_GCGC'C Cuts at: 0 1799 3678 Size: 1799 1879 BbsI GAAGACnn'nnnn_ Cuts at: 0 752 2483 2898 3173 3678 Size: 752 1731 415 275 505 Fragments arranged by size: 1731 752 505 415 275 BbvCI CC'TCA_GC Cuts at: 0 1782 3678 Size: 1782 1896 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3008 3042 3678 Size: 3008 34 636 Fragments arranged by size: 3008 636 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1026 3678 Size: 1026 2652 BclI T'GATC_A Cuts at: 0 2112 3678 Size: 2112 1566 BfrBI ATG'CAT Cuts at: 0 76 2656 3678 Size: 76 2580 1022 Fragments arranged by size: 2580 1022 76 BglI GCCn_nnn'nGGC Cuts at: 0 607 1612 1764 3678 Size: 607 1005 152 1914 Fragments arranged by size: 1914 1005 607 152 BglII A'GATC_T Cuts at: 0 880 3678 Size: 880 2798 BlpI GC'TnA_GC Cuts at: 0 1114 1963 3678 Size: 1114 849 1715 Fragments arranged by size: 1715 1114 849 Bme1580I G_kGCm'C Cuts at: 0 458 779 1703 2163 3678 Size: 458 321 924 460 1515 Fragments arranged by size: 1515 924 460 458 321 BmgBI CAC'GTC Cuts at: 0 3483 3678 Size: 3483 195 BmrI ACTGGGnnnn_n' Cuts at: 0 3531 3678 Size: 3531 147 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1178 1308 1338 1797 1941 3678 Size: 1178 130 30 459 144 1737 Fragments arranged by size: 1737 1178 459 144 130 30 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 10 905 1257 1308 1344 2062 2233 2846 Size: 10 895 352 51 36 718 171 613 Cuts at: 2846 3678 Size: 832 Fragments arranged by size: 895 832 718 613 352 171 51 36 10 Bpu10I CC'TnA_GC Cuts at: 0 746 1782 3678 Size: 746 1036 1896 Fragments arranged by size: 1896 1036 746 BsaI GGTCTCn'nnnn_ Cuts at: 0 1303 2297 2777 2864 3398 3678 Size: 1303 994 480 87 534 280 Fragments arranged by size: 1303 994 534 480 280 87 BsaHI Gr'CG_yC Cuts at: 0 1508 1796 3678 Size: 1508 288 1882 Fragments arranged by size: 1882 1508 288 BsaWI w'CCGG_w Cuts at: 0 200 1294 3678 Size: 200 1094 2384 Fragments arranged by size: 2384 1094 200 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 509 539 2206 2236 3678 Size: 509 30 1667 30 1442 Fragments arranged by size: 1667 1442 509 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 886 1144 1260 1600 1831 1917 2013 2016 Size: 886 258 116 340 231 86 96 3 Cuts at: 2016 2071 2091 2230 3678 Size: 55 20 139 1448 Fragments arranged by size: 1448 886 340 258 231 139 116 96 86 55 20 3 BseYI C'CCAG_C Cuts at: 0 451 670 1546 1839 2404 3678 Size: 451 219 876 293 565 1274 Fragments arranged by size: 1274 876 565 451 293 219 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 433 765 3490 3586 3631 3678 Size: 433 332 2725 96 45 47 Fragments arranged by size: 2725 433 332 96 47 45 BsiEI CG_ry'CG Cuts at: 0 695 1658 1829 2632 2842 3678 Size: 695 963 171 803 210 836 Fragments arranged by size: 963 836 803 695 210 171 BsiHKAI G_wGCw'C Cuts at: 0 779 1576 3113 3340 3620 3678 Size: 779 797 1537 227 280 58 Fragments arranged by size: 1537 797 779 280 227 58 Bsp1286I G_dGCh'C Cuts at: 0 167 354 458 499 779 1576 1703 1823 Size: 167 187 104 41 280 797 127 120 Cuts at: 1823 1871 2163 3113 3340 3620 3678 Size: 48 292 950 227 280 58 Fragments arranged by size: 950 797 292 280 280 227 187 167 127 120 104 58 48 41 BspEI T'CCGG_A Cuts at: 0 1294 3678 Size: 1294 2384 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 606 1945 2351 2433 3678 Size: 606 1339 406 82 1245 Fragments arranged by size: 1339 1245 606 406 82 BsrDI GCAATG_nn' Cuts at: 0 567 2984 3056 3678 Size: 567 2417 72 622 Fragments arranged by size: 2417 622 567 72 BsrFI r'CCGG_y Cuts at: 0 1377 1808 3591 3678 Size: 1377 431 1783 87 Fragments arranged by size: 1783 1377 431 87 BstAPI GCAn_nnn'nTGC Cuts at: 0 3654 3678 Size: 3654 24 BstEII G'GTnAC_C Cuts at: 0 3594 3678 Size: 3594 84 BstXI CCAn_nnnn'nTGG Cuts at: 0 1519 2211 3317 3387 3678 Size: 1519 692 1106 70 291 Fragments arranged by size: 1519 1106 692 291 70 BstYI r'GATC_y Cuts at: 0 203 256 880 1297 2330 3576 3678 Size: 203 53 624 417 1033 1246 102 Fragments arranged by size: 1246 1033 624 417 203 102 53 Bsu36I CC'TnA_GG Cuts at: 0 2750 3678 Size: 2750 928 BtgI C'CryG_G Cuts at: 0 602 1187 1464 1652 1658 1664 1712 1745 Size: 602 585 277 188 6 6 48 33 Cuts at: 1745 1823 1829 2084 2854 3065 3316 3386 3678 Size: 78 6 255 770 211 251 70 292 Fragments arranged by size: 770 602 585 292 277 255 251 211 188 78 70 48 33 6 6 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2184 3678 Size: 2184 1494 BtsI GCAGTG_nn' Cuts at: 0 777 1125 1703 2154 2568 2931 3678 Size: 777 348 578 451 414 363 747 Fragments arranged by size: 777 747 578 451 414 363 348 ClaI AT'CG_AT Cuts at: 0 323 3678 Size: 323 3355 DraIII CAC_nnn'GTG Cuts at: 0 52 3678 Size: 52 3626 DrdI GACnn_nn'nnGTC Cuts at: 0 2007 2322 3678 Size: 2007 315 1356 Fragments arranged by size: 2007 1356 315 EaeI y'GGCC_r Cuts at: 0 471 608 717 1655 1661 1826 2244 2534 Size: 471 137 109 938 6 165 418 290 Cuts at: 2534 2839 2874 3473 3530 3542 3678 Size: 305 35 599 57 12 136 Fragments arranged by size: 938 599 471 418 305 290 165 137 136 109 57 35 12 6 EagI C'GGCC_G Cuts at: 0 1655 1826 2839 3678 Size: 1655 171 1013 839 Fragments arranged by size: 1655 1013 839 171 EarI CTCTTCn'nnn_ Cuts at: 0 160 1121 2529 2739 3678 Size: 160 961 1408 210 939 Fragments arranged by size: 1408 961 939 210 160 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2795 3234 3678 Size: 2795 439 444 Fragments arranged by size: 2795 444 439 EcoICRI GAG'CTC Cuts at: 0 3111 3678 Size: 3111 567 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 10 141 562 772 905 1257 1308 1344 Size: 10 131 421 210 133 352 51 36 Cuts at: 1344 2062 2143 2233 2711 2846 3035 3678 Size: 718 81 90 478 135 189 643 Fragments arranged by size: 718 643 478 421 352 210 189 135 133 131 90 81 51 36 10 EcoNI CCTnn'n_nnAGG Cuts at: 0 25 2347 3544 3678 Size: 25 2322 1197 134 Fragments arranged by size: 2322 1197 134 25 EcoO109I rG'GnC_Cy Cuts at: 0 36 506 729 1614 1778 1883 2159 2160 Size: 36 470 223 885 164 105 276 1 Cuts at: 2160 3061 3148 3642 3678 Size: 901 87 494 36 Fragments arranged by size: 901 885 494 470 276 223 164 105 87 36 36 1 EcoRI G'AATT_C Cuts at: 0 3559 3678 Size: 3559 119 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1123 3678 Size: 1123 2555 HaeII r_GCGC'y Cuts at: 0 1187 1799 1950 2372 3422 3668 3678 Size: 1187 612 151 422 1050 246 10 Fragments arranged by size: 1187 1050 612 422 246 151 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 28 60 178 210 865 897 1990 1996 Size: 28 32 118 32 655 32 1093 6 Cuts at: 1996 2022 2028 2029 2061 2275 2277 2307 2309 Size: 26 6 1 32 214 2 30 2 Cuts at: 2309 3678 Size: 1369 Fragments arranged by size: 1369 1093 655 214 118 32 32 32 32 30 28 26 6 6 2 2 1 HincII GTy'rAC Cuts at: 0 3434 3678 Size: 3434 244 KasI G'GCGC_C Cuts at: 0 1795 3678 Size: 1795 1883 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 221 258 2027 2060 2894 3434 3678 Size: 221 37 1769 33 834 540 244 Fragments arranged by size: 1769 834 540 244 221 37 33 MscI TGG'CCA Cuts at: 0 2536 3544 3678 Size: 2536 1008 134 Fragments arranged by size: 2536 1008 134 MslI CAynn'nnrTG Cuts at: 0 3315 3678 Size: 3315 363 MspA1I CmG'CkG Cuts at: 0 403 626 899 1256 1401 1654 1714 1747 Size: 403 223 273 357 145 253 60 33 Cuts at: 1747 1825 1831 2134 2766 3678 Size: 78 6 303 632 912 Fragments arranged by size: 912 632 403 357 303 273 253 223 145 78 60 33 6 NarI GG'CG_CC Cuts at: 0 1796 3678 Size: 1796 1882 NcoI C'CATG_G Cuts at: 0 602 3065 3678 Size: 602 2463 613 Fragments arranged by size: 2463 613 602 NotI GC'GGCC_GC Cuts at: 0 1826 3678 Size: 1826 1852 NsiI A_TGCA'T Cuts at: 0 78 2658 3678 Size: 78 2580 1020 Fragments arranged by size: 2580 1020 78 PasI CC'CwG_GG Cuts at: 0 1618 2505 3678 Size: 1618 887 1173 Fragments arranged by size: 1618 1173 887 PflMI CCAn_nnn'nTGG Cuts at: 0 1072 3468 3678 Size: 1072 2396 210 Fragments arranged by size: 2396 1072 210 PfoI T'CCnGG_A Cuts at: 0 26 917 1511 2104 2326 3678 Size: 26 891 594 593 222 1352 Fragments arranged by size: 1352 891 594 593 222 26 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 349 381 2206 2238 3678 Size: 349 32 1825 32 1440 Fragments arranged by size: 1825 1440 349 32 32 PpuMI rG'GwC_Cy Cuts at: 0 36 506 1778 3678 Size: 36 470 1272 1900 Fragments arranged by size: 1900 1272 470 36 PshAI GACnn'nnGTC Cuts at: 0 3439 3678 Size: 3439 239 PspOMI G'GGCC_C Cuts at: 0 2159 3678 Size: 2159 1519 PspXI vC'TCGA_Gb Cuts at: 0 40 3678 Size: 40 3638 PstI C_TGCA'G Cuts at: 0 1210 2430 2771 3678 Size: 1210 1220 341 907 Fragments arranged by size: 1220 1210 907 341 PvuII CAG'CTG Cuts at: 0 403 899 1401 2766 3678 Size: 403 496 502 1365 912 Fragments arranged by size: 1365 912 502 496 403 SacI G_AGCT'C Cuts at: 0 3113 3678 Size: 3113 565 SacII CC_GC'GG Cuts at: 0 1655 1715 1748 1826 1832 3678 Size: 1655 60 33 78 6 1846 Fragments arranged by size: 1846 1655 78 60 33 6 SalI G'TCGA_C Cuts at: 0 3432 3678 Size: 3432 246 SapI GCTCTTCn'nnn_ Cuts at: 0 2529 3678 Size: 2529 1149 ScaI AGT'ACT Cuts at: 0 1486 3631 3678 Size: 1486 2145 47 Fragments arranged by size: 2145 1486 47 SexAI A'CCwGG_T Cuts at: 0 3620 3678 Size: 3620 58 SfcI C'TryA_G Cuts at: 0 375 1206 2165 2426 2484 2767 3678 Size: 375 831 959 261 58 283 911 Fragments arranged by size: 959 911 831 375 283 261 58 SfoI GGC'GCC Cuts at: 0 1797 3678 Size: 1797 1881 SmlI C'TyrA_G Cuts at: 0 40 1108 3678 Size: 40 1068 2570 Fragments arranged by size: 2570 1068 40 StyI C'CwwG_G Cuts at: 0 602 830 1597 1985 2155 2204 2381 3065 Size: 602 228 767 388 170 49 177 684 Cuts at: 3065 3545 3678 Size: 480 133 Fragments arranged by size: 767 684 602 480 388 228 177 170 133 49 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 473 1327 2618 3678 Size: 473 854 1291 1060 Fragments arranged by size: 1291 1060 854 473 TatI w'GTAC_w Cuts at: 0 1484 3212 3629 3678 Size: 1484 1728 417 49 Fragments arranged by size: 1728 1484 417 49 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2178 2210 3443 3475 3678 Size: 2178 32 1233 32 203 Fragments arranged by size: 2178 1233 203 32 32 Tth111I GACn'n_nGTC Cuts at: 0 2004 3678 Size: 2004 1674 XhoI C'TCGA_G Cuts at: 0 40 3678 Size: 40 3638 XmnI GAAnn'nnTTC Cuts at: 0 2819 3678 Size: 2819 859 ZraI GAC'GTC Cuts at: 0 1509 3678 Size: 1509 2169 Enzymes that do cut: AarI AatII AccI AcuI AflII AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrDI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NarI NcoI NotI NsiI PasI PflMI PfoI PpiI PpuMI PshAI PspOMI PspXI PstI PvuII SacI SacII SalI SapI ScaI SexAI SfcI SfoI SmlI StyI TaqII TatI TstI Tth111I XhoI XmnI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflIII AgeI AleI AscI AseI AsiSI BaeI BmtI BpuEI BsaAI BsaBI BsiWI BsmI BsmBI BspHI BsrBI BsrGI BssHII BssSI BstBI BstZ17I CspCI DraI EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI NruI NspI PacI PciI PmeI PmlI PsiI PsrI PvuI RsrII SanDI SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI XcmI XmaI