Restriction map for
pFN21AE9393
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# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AclI AflIII AgeI Aor51HI
AscI AseI AsnI Asp718I AviII
BbrPI BsaAI BsaBI BsiWI BsmBI
BsmI Bsp1407I BspHI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstZ17I CpoI DraI DraIII Eco47III
EcoRV FseI FspI HindIII HpaI
KpnI MamI MfeI MluI MunI
NaeI NdeI NgoMIV NheI NruI
NspI NspV PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SbfI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 1582 GACGTC GACGT^C
AccI 3507 GTCGAC GT^[AC][GT]AC
AccIII 1369 TCCGGA T^CCGGA
AflII 1183 CTTAAG C^TTAAG
Alw44I 850 GTGCAC G^TGCAC
AocI 2824 CCTAAGG CC^T[ACGT]AGG
ApaI 2234 GGGCCC GGGCC^C
ApaLI 850 GTGCAC G^TGCAC
Asp700I 2890 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2076 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2230 CCTAGG C^CTAGG
BbeI 1870 GGCGCC GGCGC^C
BbvCI 1856 CCTCAGC CCTCAGC(-5/-2)
BcgI 3094 GCAGCATTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1090 GTATCC GTATCC(6/5)
BclI 2187 TGATCA T^GATCA
BfrI 1183 CTTAAG C^TTAAG
BglII 955 AGATCT A^GATCT
BlnI 2230 CCTAGG C^CTAGG
BseAI 1369 TCCGGA T^CCGGA
BspDI 397 ATCGAT AT^CGAT
BspEI 1369 TCCGGA T^CCGGA
BstAPI 3723 GCACTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3669 GGTCACC G^GT[ACGT]ACC
BstPI 3669 GGTCACC G^GT[ACGT]ACC
Bsu36I 2824 CCTAAGG CC^T[ACGT]AGG
ClaI 397 ATCGAT AT^CGAT
Eco81I 2824 CCTAAGG CC^T[ACGT]AGG
EcoO65I 3669 GGTCACC G^GT[ACGT]ACC
EcoRI 3634 GAATTC G^AATTC
FbaI 2187 TGATCA T^GATCA
HgaI 1909 GACGC GACGC(5/10)
HincII 3507 GTCGAC GT[CT]^[GA]AC
HindII 3507 GTCGAC GT[CT]^[GA]AC
KasI 1870 GGCGCC G^GCGCC
MroI 1369 TCCGGA T^CCGGA
MslI 3386 CATCTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1870 GGCGCC GG^CGCC
NotI 1900 GCGGCCGC GC^GGCCGC
PaeR7I 40 CTCGAG C^TCGAG
PflFI 2076 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3510 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 3184 GAGCTC GAGCT^C
SalI 3507 GTCGAC G^TCGAC
SapI 2597 GCTCTTC GCTCTTC(1/4)
SexAI 3695 ACCTGGT A^CC[AT]GGT
SfoI 1870 GGCGCC GGC^GCC
Tth111I 2076 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 40 CTCGAG C^TCGAG
XmnI 2890 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcyI 1582 GACGTC G[GA]^CG[CT]C
1870 GGCGCC
AhdI 37 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2113 GACCTGGAGTC
AspEI 37 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2113 GACCTGGAGTC
BalI 2609 TGGCCA TGG^CCA
3617 TGGCCA
BamHI 278 GGATCC G^GATCC
2405 GGATCC
BlpI 1188 GCTAAGC GC^T[ACGT]AGC
2037 GCTAAGC
Bpu1102I 1188 GCTAAGC GC^T[ACGT]AGC
2037 GCTAAGC
BsaHI 1582 GACGTC G[GA]^CG[CT]C
1870 GGCGCC
BsaWI 275 ACCGGA [AT]^CCGG[AT]
1369 TCCGGA
CelII 1188 GCTAAGC GC^T[ACGT]AGC
2037 GCTAAGC
DrdI 2076 GACAGAGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2391 GACCCTCCTGTC
Eam1105I 37 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2113 GACCTGGAGTC
EcoT22I 149 ATGCAT ATGCA^T
2729 ATGCAT
Hin1I 1582 GACGTC G[GA]^CG[CT]C
1870 GGCGCC
MluNI 2609 TGGCCA TGG^CCA
3617 TGGCCA
MscI 2609 TGGCCA TGG^CCA
3617 TGGCCA
NcoI 677 CCATGG C^CATGG
3140 CCATGG
NsiI 149 ATGCAT ATGCA^T
2729 ATGCAT
PflMI 1141 CCAGACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3537 CCACCAGATGG
ScaI 1559 AGTACT AGT^ACT
3704 AGTACT
SmlI 40 CTCGAG C^T[CT][GA]AG
1183 CTTAAG
TfiI 418 GATTC G^A[AT]TC
3145 GAATC
Van91I 1141 CCAGACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3537 CCACCAGATGG
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# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII