Restriction map for
pFN21AE9393
(Circular) (Six-base) MAPSORT of: pFN21AE9393.seq Check: 7469 from: 1 to: 8458
pFN21AE9393 8458 bp
With 182 enzymes: SgfI *
December 8, 2014 13:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2686 4431 4513 2686
Size: 1745 82 6631
Fragments arranged by size:
6631 1745 82
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3591 6268 278
Size: 53 83 186 936 2055 2677 2468
Fragments arranged by size:
2677 2468 2055 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5513 5801 1937
Size: 3576 288 4594
Fragments arranged by size:
4594 3576 288
Acc65I G'GTAC_C
Cuts at: 5779 5779
Size: 8458
AclI AA'CG_TT
Cuts at: 1563 6585 6958 1563
Size: 5022 373 3063
Fragments arranged by size:
5022 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2221 2642 2852 4223 4791 5115 6521 7905
Size: 589 421 210 1371 568 324 1406 1384
Cuts at: 7905 8173 1632
Size: 268 1917
Fragments arranged by size:
1917 1406 1384 1371 589 568 421 324
268 210
AflII C'TTAA_G
Cuts at: 820 1017 3188 820
Size: 197 2171 6090
Fragments arranged by size:
6090 2171 197
AflIII A'CryG_T
Cuts at: 1203 7357 8334 1203
Size: 6154 977 1327
Fragments arranged by size:
6154 1327 977
AgeI A'CCGG_T
Cuts at: 7289 7289
Size: 8458
AhdI GACnn_n'nnGTC
Cuts at: 2047 4123 7187 2047
Size: 2076 3064 3318
Fragments arranged by size:
3318 3064 2076
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8458
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4016 4666 1184
Size: 2832 650 4976
Fragments arranged by size:
4976 2832 650
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2700 2732 4286 4318 2700
Size: 32 1554 32 6840
Fragments arranged by size:
6840 1554 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2483 3966 4201 4965 5725 6201 7773 2483
Size: 1483 235 764 760 476 1572 3168
Fragments arranged by size:
3168 1572 1483 764 760 476 235
ApaI G_GGCC'C
Cuts at: 4243 4243
Size: 8458
ApaLI G'TGCA_C
Cuts at: 2855 6515 7671 2855
Size: 3660 1156 3642
Fragments arranged by size:
3660 3642 1156
ApoI r'AATT_y
Cuts at: 1493 2102 3062 5639 5767 5993 6145 8254 1493
Size: 609 960 2577 128 226 152 2109 1697
Fragments arranged by size:
2577 2109 1697 960 609 226 152 128
AseI AT'TA_AT
Cuts at: 160 1032 7012 160
Size: 872 5980 1606
Fragments arranged by size:
5980 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8458
AvaI C'yCGr_G
Cuts at: 1870 1943 2045 2622 3893 5783 1870
Size: 73 102 577 1271 1890 4545
Fragments arranged by size:
4545 1890 1271 577 102 73
AvrII C'CTAG_G
Cuts at: 4235 4235
Size: 8458
BamHI G'GATC_C
Cuts at: 2283 4410 5788 6155 2283
Size: 2127 1378 367 4586
Fragments arranged by size:
4586 2127 1378 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2535 3298 3875 5779 7234
Size: 325 217 627 748 763 577 1904 1455
Cuts at: 7234 618
Size: 1842
Fragments arranged by size:
1904 1842 1455 763 748 627 577 325
217
BanII G_rGCy'C
Cuts at: 1391 2247 2434 2579 3903 3951 4243 5193 5777
Size: 856 187 145 1324 48 292 950 584
Cuts at: 5777 1391
Size: 4072
Fragments arranged by size:
4072 1324 950 856 584 292 187 145
48
BbeI G_GCGC'C
Cuts at: 3879 3879
Size: 8458
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2070 2832 4563 4978 5253 928
Size: 971 171 762 1731 415 275 4133
Fragments arranged by size:
4133 1731 971 762 415 275 171
BbvCI CC'TCA_GC
Cuts at: 3862 3862
Size: 8458
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5088 5122 6649 6683 1903
Size: 34 3151 34 1527 34 3678
Fragments arranged by size:
3678 3151 1527 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3106 6350 7560 3106
Size: 3244 1210 4004
Fragments arranged by size:
4004 3244 1210
BclI T'GATC_A
Cuts at: 1548 4192 1548
Size: 2644 5814
BfrBI ATG'CAT
Cuts at: 2156 4736 2156
Size: 2580 5878
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2687 3692 3844
Size: 107 122 71 631 605 1015 1005 152
Cuts at: 3844 7069 136
Size: 3225 1525
Fragments arranged by size:
3225 1525 1015 1005 631 605 152 122
107 71
BglII A'GATC_T
Cuts at: 2960 8453 2960
Size: 5493 2965
BlpI GC'TnA_GC
Cuts at: 3194 4043 5875 3194
Size: 849 1832 5777
Fragments arranged by size:
5777 1832 849
Bme1580I G_kGCm'C
Cuts at: 1790 2538 2859 3783 4243 6519 7675 1790
Size: 748 321 924 460 2276 1156 2573
Fragments arranged by size:
2573 2276 1156 924 748 460 321
BmgBI CAC'GTC
Cuts at: 1318 5563 1318
Size: 4245 4213
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5611 6189 7147
Size: 416 53 83 399 24 4194 578 958
Cuts at: 7147 442
Size: 1753
Fragments arranged by size:
4194 1753 958 578 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8458
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3258 3388 3418 3877 4021 3258
Size: 130 30 459 144 7695
Fragments arranged by size:
7695 459 144 130 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2015 2985 3337 3388 3424 4142 4313 4926
Size: 776 970 352 51 36 718 171 613
Cuts at: 4926 7118 1239
Size: 2192 2579
Fragments arranged by size:
2579 2192 970 776 718 613 352 171
51 36
Bpu10I CC'TnA_GC
Cuts at: 2826 3862 2826
Size: 1036 7422
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6583 7448 7746 6583
Size: 865 298 7295
Fragments arranged by size:
7295 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3383 4377 4857 4944 5478 7121 882
Size: 617 1884 994 480 87 534 1643 2219
Fragments arranged by size:
2219 1884 1643 994 617 534 480 87
BsaAI yAC'GTr
Cuts at: 493 1204 6249 493
Size: 711 5045 2702
Fragments arranged by size:
5045 2702 711
BsaBI GATnn'nnATC
Cuts at: 8452 8452
Size: 8458
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3588 3876 6265 6647
Size: 53 83 186 936 2055 288 2389 382
Cuts at: 6647 7282 275
Size: 635 1451
Fragments arranged by size:
2389 2055 1451 936 635 382 288 186
83 53
BsaWI w'CCGG_w
Cuts at: 2280 3374 6891 7289 7563 7710 8121 2280
Size: 1094 3517 398 274 147 411 2617
Fragments arranged by size:
3517 2617 1094 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2589 2619 4286 4316 1178
Size: 30 165 30 1186 30 1667 30 5320
Fragments arranged by size:
5320 1667 1186 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2966 3224 3340 3680 3911 3997 4093 4096
Size: 1780 258 116 340 231 86 96 3
Cuts at: 4096 4151 4171 4310 1186
Size: 55 20 139 5334
Fragments arranged by size:
5334 1780 340 258 231 139 116 96
86 55 20 3
BseYI C'CCAG_C
Cuts at: 2531 2750 3626 3919 4484 7661 8157 2531
Size: 219 876 293 565 3177 496 2832
Fragments arranged by size:
3177 2832 876 565 496 293 219
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2513 2845 5570 5666 5711 1163
Size: 573 777 332 2725 96 45 3910
Fragments arranged by size:
3910 2725 777 573 332 96 45
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2775 3738 3909 4712 4922 5908
Size: 275 186 774 963 171 803 210 986
Cuts at: 5908 6669 7697 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 986 963 803 774 761 275
210 186 171
BsiHKAI G_wGCw'C
Cuts at: 2859 3656 5193 5420 5700 6519 6604 7675 2859
Size: 797 1537 227 280 819 85 1071 3642
Fragments arranged by size:
3642 1537 1071 819 797 280 227 85
BsmI GAATG_Cn'
Cuts at: 5969 6062 5969
Size: 93 8365
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2247 2434 2538 2579 2859 3656 3783
Size: 399 457 187 104 41 280 797 127
Cuts at: 3783 3903 3951 4243 5193 5420 5700 5777 6519
Size: 120 48 292 950 227 280 77 742
Cuts at: 6519 6604 7675 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 950 797 742 457 399 292
280 280 227 187 127 120 104 85
77 48 41
BspEI T'CCGG_A
Cuts at: 3374 8121 3374
Size: 4747 3711
BspHI T'CATG_A
Cuts at: 6347 8077 6347
Size: 1730 6728
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2686 4025 4431 4513 5813 844
Size: 1842 1339 406 82 1300 3489
Fragments arranged by size:
3489 1842 1339 1300 406 82
BsrBI CCG'CTC
Cuts at: 1394 1823 6345 1394
Size: 429 4522 3507
Fragments arranged by size:
4522 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2647 5064 5136 6953 7127 65
Size: 1183 1399 2417 72 1817 174 1396
Fragments arranged by size:
2417 1817 1399 1396 1183 174 72
BsrFI r'CCGG_y
Cuts at: 1700 1810 3457 3888 5671 7102 7289 1700
Size: 110 1647 431 1783 1431 187 2869
Fragments arranged by size:
2869 1783 1647 1431 431 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8458
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8458
BssSI C'ACGA_G
Cuts at: 6518 7530 6518
Size: 1012 7446
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5734 794
Size: 4940 3518
BstBI TT'CG_AA
Cuts at: 7262 7262
Size: 8458
BstEII G'GTnAC_C
Cuts at: 1184 5674 1184
Size: 4490 3968
BstXI CCAn_nnnn'nTGG
Cuts at: 3599 4291 5397 5467 3599
Size: 692 1106 70 6590
Fragments arranged by size:
6590 1106 692 70
BstYI r'GATC_y
Cuts at: 1972 2283 2336 2960 3377 4410 5656 5788 6155
Size: 311 53 624 417 1033 1246 132 367
Cuts at: 6155 6540 6557 8009 8095 8107 8453 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1246 1033 624 417 385 367
346 311 132 86 53 17 12
Bsu36I CC'TnA_GG
Cuts at: 4830 4830
Size: 8458
BtgI C'CryG_G
Cuts at: 513 1065 2004 2682 3267 3544 3732 3738 3744
Size: 552 939 678 585 277 188 6 6
Cuts at: 3744 3792 3825 3903 3909 4164 4934 5145 5396
Size: 48 33 78 6 255 770 211 251
Cuts at: 5396 5466 513
Size: 70 3505
Fragments arranged by size:
3505 939 770 678 585 552 277 255
251 211 188 78 70 48 33 6
6 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4264 487
Size: 683 56 3038 4681
Fragments arranged by size:
4681 3038 683 56
BtsI GCAGTG_nn'
Cuts at: 2857 3205 3783 4234 4648 5011 5974 6768 6788
Size: 348 578 451 414 363 963 794 20
Cuts at: 6788 2857
Size: 4527
Fragments arranged by size:
4527 963 794 578 451 414 363 348
20
ClaI AT'CG_AT
Cuts at: 1554 2403 1554
Size: 849 7609
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8423
DraI TTT'AAA
Cuts at: 5762 5762
Size: 8458
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4087 4402 7465 809
Size: 507 2771 315 3063 1802
Fragments arranged by size:
3063 2771 1802 507 315
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2551 2688 2797
Size: 54 1425 50 275 18 721 137 109
Cuts at: 2797 3735 3741 3906 4324 4614 4919 4954 5553
Size: 938 6 165 418 290 305 35 599
Cuts at: 5553 5610 5622 5905 6794 8423 8
Size: 57 12 283 889 1629 43
Fragments arranged by size:
1629 1425 938 889 721 599 418 305
290 283 275 165 137 109 57 54
50 43 35 18 12 6
EagI C'GGCC_G
Cuts at: 1537 1812 3735 3906 4919 5905 1537
Size: 275 1923 171 1013 986 4090
Fragments arranged by size:
4090 1923 1013 986 275 171
EarI CTCTTCn'nnn_
Cuts at: 1353 2240 3201 4609 4819 6388 1353
Size: 887 961 1408 210 1569 3423
Fragments arranged by size:
3423 1569 1408 961 887 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4875 5314 7045 7419 7565 387
Size: 1064 138 3286 439 1731 374 146 1280
Fragments arranged by size:
3286 1731 1280 1064 439 374 146 138
EcoICRI GAG'CTC
Cuts at: 5191 5191
Size: 8458
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2015 2221 2642 2852 2985 3337 3388
Size: 393 383 206 421 210 133 352 51
Cuts at: 3388 3424 4142 4223 4313 4791 4926 5115 6521
Size: 36 718 81 90 478 135 189 1406
Cuts at: 6521 7118 7905 8173 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1406 787 718 597 478 421 393
383 352 268 210 206 189 135 133
90 81 51 36
EcoNI CCTnn'n_nnAGG
Cuts at: 2030 4427 5624 2030
Size: 2397 1197 4864
Fragments arranged by size:
4864 2397 1197
EcoO109I rG'GnC_Cy
Cuts at: 2041 2586 2809 3694 3858 3963 4239 4240 5141
Size: 545 223 885 164 105 276 1 901
Cuts at: 5141 5228 5722 2041
Size: 87 494 4777
Fragments arranged by size:
4777 901 885 545 494 276 223 164
105 87 1
EcoRI G'AATT_C
Cuts at: 5639 5767 6145 5639
Size: 128 378 7952
Fragments arranged by size:
7952 378 128
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3203 3203
Size: 8458
FspI TGC'GCA
Cuts at: 6964 6964
Size: 8458
HaeII r_GCGC'y
Cuts at: 1678 3267 3879 4030 4452 5502 5748 7605 1678
Size: 1589 612 151 422 1050 246 1857 2531
Fragments arranged by size:
2531 1857 1589 1050 612 422 246 151
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2033 2065 2258 2290 2945
Size: 32 135 32 461 32 193 32 655
Cuts at: 2945 2977 4070 4076 4102 4108 4109 4141 4355
Size: 32 1093 6 26 6 1 32 214
Cuts at: 4355 4357 4387 4389 7099 7131 7173 7205 1373
Size: 2 30 2 2710 32 42 32 2626
Fragments arranged by size:
2710 2626 1093 655 461 214 193 135
42 32 32 32 32 32 32 32
32 30 26 6 6 2 2 1
HincII GTy'rAC
Cuts at: 669 1657 1938 5514 5802 6048 669
Size: 988 281 3576 288 246 3079
Fragments arranged by size:
3576 3079 988 288 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8458
HpaI GTT'AAC
Cuts at: 6048 6048
Size: 8458
KasI G'GCGC_C
Cuts at: 3875 3875
Size: 8458
KpnI G_GTAC'C
Cuts at: 5783 5783
Size: 8458
MfeI C'AATT_G
Cuts at: 6057 6057
Size: 8458
MluI A'CGCG_T
Cuts at: 8334 8334
Size: 8458
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2301 2338 4107 4140 4974 5514 7572 7756
Size: 996 37 1769 33 834 540 2058 184
Cuts at: 7756 1305
Size: 2007
Fragments arranged by size:
2058 2007 1769 996 834 540 184 37
33
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4616 5624 10
Size: 54 1425 343 2784 1008 2844
Fragments arranged by size:
2844 2784 1425 1008 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5395 6416 6775 6934 518
Size: 648 4229 1021 359 159 2042
Fragments arranged by size:
4229 2042 1021 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2483 2706 2979 3336 3481 3734 3794 3827
Size: 877 223 273 357 145 253 60 33
Cuts at: 3827 3905 3911 4214 4846 5874 6551 7699 7944
Size: 78 6 303 632 1028 677 1148 245
Cuts at: 7944 1606
Size: 2120
Fragments arranged by size:
2120 1148 1028 877 677 632 357 303
273 253 245 223 145 78 60 33
6
NarI GG'CG_CC
Cuts at: 3876 3876
Size: 8458
NcoI C'CATG_G
Cuts at: 513 1065 2004 2682 5145 513
Size: 552 939 678 2463 3826
Fragments arranged by size:
3826 2463 939 678 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8458
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8458
NotI GC'GGCC_GC
Cuts at: 3906 5905 3906
Size: 1999 6459
NsiI A_TGCA'T
Cuts at: 2158 4738 2158
Size: 2580 5878
NspI r_CATG'y
Cuts at: 1132 1229 5816 7361 1132
Size: 97 4587 1545 2229
Fragments arranged by size:
4587 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3698 4585 1348
Size: 2350 887 5221
Fragments arranged by size:
5221 2350 887
PciI A'CATG_T
Cuts at: 7357 7357
Size: 8458
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3152 5548 1108
Size: 2044 2396 4018
Fragments arranged by size:
4018 2396 2044
PfoI T'CCnGG_A
Cuts at: 2031 2997 3591 4184 4406 2031
Size: 966 594 593 222 6083
Fragments arranged by size:
6083 966 594 593 222
PmeI GTTT'AAAC
Cuts at: 5762 5762
Size: 8458
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2429 2461 4286 4318 6527 6559 8040 8072 2429
Size: 32 1825 32 2209 32 1481 32 2815
Fragments arranged by size:
2815 2209 1825 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2041 2586 3858 2041
Size: 545 1272 6641
Fragments arranged by size:
6641 1272 545
PshAI GACnn'nnGTC
Cuts at: 1613 5519 1613
Size: 3906 4552
PsiI TTA'TAA
Cuts at: 6028 6028
Size: 8458
PspOMI G'GGCC_C
Cuts at: 4239 4239
Size: 8458
PspXI vC'TCGA_Gb
Cuts at: 2045 2045
Size: 8458
PstI C_TGCA'G
Cuts at: 830 3290 4510 4851 5810 830
Size: 2460 1220 341 959 3478
Fragments arranged by size:
3478 2460 1220 959 341
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8458
PvuII CAG'CTG
Cuts at: 2483 2979 3481 4846 2483
Size: 496 502 1365 6095
Fragments arranged by size:
6095 1365 502 496
SacI G_AGCT'C
Cuts at: 5193 5193
Size: 8458
SacII CC_GC'GG
Cuts at: 3735 3795 3828 3906 3912 3735
Size: 60 33 78 6 8281
Fragments arranged by size:
8281 78 60 33 6
SalI G'TCGA_C
Cuts at: 1936 5512 5800 1936
Size: 3576 288 4594
Fragments arranged by size:
4594 3576 288
SapI GCTCTTCn'nnn_
Cuts at: 4609 4609
Size: 8458
SbfI CC_TGCA'GG
Cuts at: 5810 5810
Size: 8458
ScaI AGT'ACT
Cuts at: 3566 5711 6706 8418 3566
Size: 2145 995 1712 3606
Fragments arranged by size:
3606 2145 1712 995
SexAI A'CCwGG_T
Cuts at: 5700 5700
Size: 8458
SfcI C'TryA_G
Cuts at: 826 1045 2455 3286 4245 4506 4564 4847 5806
Size: 219 1410 831 959 261 58 283 959
Cuts at: 5806 6941 7622 7813 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1410 1135 959 959 831 681 283
261 219 191 58
SfoI GGC'GCC
Cuts at: 3877 3877
Size: 8458
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8458
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8458
SmaI CCC'GGG
Cuts at: 1872 5785 1872
Size: 3913 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2045 3188 6562 7463 7725 820
Size: 197 926 102 1143 3374 901 262 1553
Fragments arranged by size:
3374 1553 1143 926 901 262 197 102
SnaBI TAC'GTA
Cuts at: 493 6249 493
Size: 5756 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8458
SphI G_CATG'C
Cuts at: 1132 5816 1132
Size: 4684 3774
SspI AAT'ATT
Cuts at: 5 52 6382 5
Size: 47 6330 2081
Fragments arranged by size:
6330 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8458
StyI C'CwwG_G
Cuts at: 513 1065 2004 2682 2910 3677 4065 4235 4284
Size: 552 939 678 228 767 388 170 49
Cuts at: 4284 4461 5145 5625 5897 513
Size: 177 684 480 272 3074
Fragments arranged by size:
3074 939 767 684 678 552 480 388
272 228 177 170 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2553 3407 4698 6485 6502 6655 6840
Size: 1255 973 854 1291 1787 17 153 185
Cuts at: 6840 7285 325
Size: 445 1498
Fragments arranged by size:
1787 1498 1291 1255 973 854 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3564 5292 5709
Size: 274 80 33 51 1443 1587 1728 417
Cuts at: 5709 6704 8416 96
Size: 995 1712 138
Fragments arranged by size:
1728 1712 1587 1443 995 417 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4258 4290 5523 5555 8418 8450 4258
Size: 32 1233 32 2863 32 4266
Fragments arranged by size:
4266 2863 1233 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4084 1316
Size: 2768 5690
XbaI T'CTAG_A
Cuts at: 5794 5794
Size: 8458
XhoI C'TCGA_G
Cuts at: 1943 2045 1943
Size: 102 8356
XmaI C'CCGG_G
Cuts at: 1870 5783 1870
Size: 3913 4545
XmnI GAAnn'nnTTC
Cuts at: 4899 6587 4899
Size: 1688 6770
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3589 6266 276
Size: 53 83 186 936 2055 2677 2468
Fragments arranged by size:
2677 2468 2055 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BsiWI BsmBI BstZ17I DraIII EcoRV
FseI FspAI NaeI NgoMIV NruI PacI PmlI PsrI
RsrII SanDI SfiI SrfI SwaI XcmI