(Linear) (Six-base) MAPSORT of: KIEE9393.seq Check: 5496 from: 1 to: 3753 KIEE9393.orf With 182 enzymes: SgfI * December 5, 2014 13:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 681 2426 2508 3753 Size: 681 1745 82 1245 Fragments arranged by size: 1745 1245 681 82 AatII G_ACGT'C Cuts at: 0 1586 3753 Size: 1586 2167 AccI GT'mk_AC Cuts at: 0 3508 3753 Size: 3508 245 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 216 637 847 2218 2786 3110 3753 Size: 216 421 210 1371 568 324 643 Fragments arranged by size: 1371 643 568 421 324 216 210 AflII C'TTAA_G Cuts at: 0 1183 3753 Size: 1183 2570 AhdI GACnn_n'nnGTC Cuts at: 0 42 2118 3753 Size: 42 2076 1635 Fragments arranged by size: 2076 1635 42 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2011 2661 3753 Size: 2011 650 1092 Fragments arranged by size: 2011 1092 650 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 695 727 2281 2313 3753 Size: 695 32 1554 32 1440 Fragments arranged by size: 1554 1440 695 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 478 1961 2196 2960 3720 3753 Size: 478 1483 235 764 760 33 Fragments arranged by size: 1483 764 760 478 235 33 ApaI G_GGCC'C Cuts at: 0 2238 3753 Size: 2238 1515 ApaLI G'TGCA_C Cuts at: 0 850 3753 Size: 850 2903 ApoI r'AATT_y Cuts at: 0 97 1057 3634 3753 Size: 97 960 2577 119 Fragments arranged by size: 2577 960 119 97 AvaI C'yCGr_G Cuts at: 0 40 617 1888 3753 Size: 40 577 1271 1865 Fragments arranged by size: 1865 1271 577 40 AvrII C'CTAG_G Cuts at: 0 2230 3753 Size: 2230 1523 BamHI G'GATC_C Cuts at: 0 278 2405 3753 Size: 278 2127 1348 Fragments arranged by size: 2127 1348 278 BanI G'GyrC_C Cuts at: 0 530 1293 1870 3753 Size: 530 763 577 1883 Fragments arranged by size: 1883 763 577 530 BanII G_rGCy'C Cuts at: 0 242 429 574 1898 1946 2238 3188 3753 Size: 242 187 145 1324 48 292 950 565 Fragments arranged by size: 1324 950 565 292 242 187 145 48 BbeI G_GCGC'C Cuts at: 0 1874 3753 Size: 1874 1879 BbsI GAAGACnn'nnnn_ Cuts at: 0 65 827 2558 2973 3248 3753 Size: 65 762 1731 415 275 505 Fragments arranged by size: 1731 762 505 415 275 65 BbvCI CC'TCA_GC Cuts at: 0 1857 3753 Size: 1857 1896 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3083 3117 3753 Size: 3083 34 636 Fragments arranged by size: 3083 636 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1101 3753 Size: 1101 2652 BclI T'GATC_A Cuts at: 0 2187 3753 Size: 2187 1566 BfrBI ATG'CAT Cuts at: 0 151 2731 3753 Size: 151 2580 1022 Fragments arranged by size: 2580 1022 151 BglI GCCn_nnn'nGGC Cuts at: 0 682 1687 1839 3753 Size: 682 1005 152 1914 Fragments arranged by size: 1914 1005 682 152 BglII A'GATC_T Cuts at: 0 955 3753 Size: 955 2798 BlpI GC'TnA_GC Cuts at: 0 1189 2038 3753 Size: 1189 849 1715 Fragments arranged by size: 1715 1189 849 Bme1580I G_kGCm'C Cuts at: 0 533 854 1778 2238 3753 Size: 533 321 924 460 1515 Fragments arranged by size: 1515 924 533 460 321 BmgBI CAC'GTC Cuts at: 0 3558 3753 Size: 3558 195 BmrI ACTGGGnnnn_n' Cuts at: 0 3606 3753 Size: 3606 147 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1253 1383 1413 1872 2016 3753 Size: 1253 130 30 459 144 1737 Fragments arranged by size: 1737 1253 459 144 130 30 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 10 980 1332 1383 1419 2137 2308 2921 Size: 10 970 352 51 36 718 171 613 Cuts at: 2921 3753 Size: 832 Fragments arranged by size: 970 832 718 613 352 171 51 36 10 Bpu10I CC'TnA_GC Cuts at: 0 821 1857 3753 Size: 821 1036 1896 Fragments arranged by size: 1896 1036 821 BsaI GGTCTCn'nnnn_ Cuts at: 0 1378 2372 2852 2939 3473 3753 Size: 1378 994 480 87 534 280 Fragments arranged by size: 1378 994 534 480 280 87 BsaHI Gr'CG_yC Cuts at: 0 1583 1871 3753 Size: 1583 288 1882 Fragments arranged by size: 1882 1583 288 BsaWI w'CCGG_w Cuts at: 0 275 1369 3753 Size: 275 1094 2384 Fragments arranged by size: 2384 1094 275 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 584 614 2281 2311 3753 Size: 584 30 1667 30 1442 Fragments arranged by size: 1667 1442 584 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 961 1219 1335 1675 1906 1992 2088 2091 Size: 961 258 116 340 231 86 96 3 Cuts at: 2091 2146 2166 2305 3753 Size: 55 20 139 1448 Fragments arranged by size: 1448 961 340 258 231 139 116 96 86 55 20 3 BseYI C'CCAG_C Cuts at: 0 526 745 1621 1914 2479 3753 Size: 526 219 876 293 565 1274 Fragments arranged by size: 1274 876 565 526 293 219 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 508 840 3565 3661 3706 3753 Size: 508 332 2725 96 45 47 Fragments arranged by size: 2725 508 332 96 47 45 BsiEI CG_ry'CG Cuts at: 0 770 1733 1904 2707 2917 3753 Size: 770 963 171 803 210 836 Fragments arranged by size: 963 836 803 770 210 171 BsiHKAI G_wGCw'C Cuts at: 0 854 1651 3188 3415 3695 3753 Size: 854 797 1537 227 280 58 Fragments arranged by size: 1537 854 797 280 227 58 Bsp1286I G_dGCh'C Cuts at: 0 242 429 533 574 854 1651 1778 1898 Size: 242 187 104 41 280 797 127 120 Cuts at: 1898 1946 2238 3188 3415 3695 3753 Size: 48 292 950 227 280 58 Fragments arranged by size: 950 797 292 280 280 242 227 187 127 120 104 58 48 41 BspEI T'CCGG_A Cuts at: 0 1369 3753 Size: 1369 2384 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 681 2020 2426 2508 3753 Size: 681 1339 406 82 1245 Fragments arranged by size: 1339 1245 681 406 82 BsrDI GCAATG_nn' Cuts at: 0 642 3059 3131 3753 Size: 642 2417 72 622 Fragments arranged by size: 2417 642 622 72 BsrFI r'CCGG_y Cuts at: 0 1452 1883 3666 3753 Size: 1452 431 1783 87 Fragments arranged by size: 1783 1452 431 87 BstAPI GCAn_nnn'nTGC Cuts at: 0 3729 3753 Size: 3729 24 BstEII G'GTnAC_C Cuts at: 0 3669 3753 Size: 3669 84 BstXI CCAn_nnnn'nTGG Cuts at: 0 1594 2286 3392 3462 3753 Size: 1594 692 1106 70 291 Fragments arranged by size: 1594 1106 692 291 70 BstYI r'GATC_y Cuts at: 0 278 331 955 1372 2405 3651 3753 Size: 278 53 624 417 1033 1246 102 Fragments arranged by size: 1246 1033 624 417 278 102 53 Bsu36I CC'TnA_GG Cuts at: 0 2825 3753 Size: 2825 928 BtgI C'CryG_G Cuts at: 0 677 1262 1539 1727 1733 1739 1787 1820 Size: 677 585 277 188 6 6 48 33 Cuts at: 1820 1898 1904 2159 2929 3140 3391 3461 3753 Size: 78 6 255 770 211 251 70 292 Fragments arranged by size: 770 677 585 292 277 255 251 211 188 78 70 48 33 6 6 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2259 3753 Size: 2259 1494 BtsI GCAGTG_nn' Cuts at: 0 852 1200 1778 2229 2643 3006 3753 Size: 852 348 578 451 414 363 747 Fragments arranged by size: 852 747 578 451 414 363 348 ClaI AT'CG_AT Cuts at: 0 398 3753 Size: 398 3355 DrdI GACnn_nn'nnGTC Cuts at: 0 2082 2397 3753 Size: 2082 315 1356 Fragments arranged by size: 2082 1356 315 EaeI y'GGCC_r Cuts at: 0 546 683 792 1730 1736 1901 2319 2609 Size: 546 137 109 938 6 165 418 290 Cuts at: 2609 2914 2949 3548 3605 3617 3753 Size: 305 35 599 57 12 136 Fragments arranged by size: 938 599 546 418 305 290 165 137 136 109 57 35 12 6 EagI C'GGCC_G Cuts at: 0 1730 1901 2914 3753 Size: 1730 171 1013 839 Fragments arranged by size: 1730 1013 839 171 EarI CTCTTCn'nnn_ Cuts at: 0 235 1196 2604 2814 3753 Size: 235 961 1408 210 939 Fragments arranged by size: 1408 961 939 235 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2870 3309 3753 Size: 2870 439 444 Fragments arranged by size: 2870 444 439 EcoICRI GAG'CTC Cuts at: 0 3186 3753 Size: 3186 567 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 10 216 637 847 980 1332 1383 1419 Size: 10 206 421 210 133 352 51 36 Cuts at: 1419 2137 2218 2308 2786 2921 3110 3753 Size: 718 81 90 478 135 189 643 Fragments arranged by size: 718 643 478 421 352 210 206 189 135 133 90 81 51 36 10 EcoNI CCTnn'n_nnAGG Cuts at: 0 25 2422 3619 3753 Size: 25 2397 1197 134 Fragments arranged by size: 2397 1197 134 25 EcoO109I rG'GnC_Cy Cuts at: 0 36 581 804 1689 1853 1958 2234 2235 Size: 36 545 223 885 164 105 276 1 Cuts at: 2235 3136 3223 3717 3753 Size: 901 87 494 36 Fragments arranged by size: 901 885 545 494 276 223 164 105 87 36 36 1 EcoRI G'AATT_C Cuts at: 0 3634 3753 Size: 3634 119 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1198 3753 Size: 1198 2555 HaeII r_GCGC'y Cuts at: 0 1262 1874 2025 2447 3497 3743 3753 Size: 1262 612 151 422 1050 246 10 Fragments arranged by size: 1262 1050 612 422 246 151 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 28 60 253 285 940 972 2065 2071 Size: 28 32 193 32 655 32 1093 6 Cuts at: 2071 2097 2103 2104 2136 2350 2352 2382 2384 Size: 26 6 1 32 214 2 30 2 Cuts at: 2384 3753 Size: 1369 Fragments arranged by size: 1369 1093 655 214 193 32 32 32 32 30 28 26 6 6 2 2 1 HincII GTy'rAC Cuts at: 0 3509 3753 Size: 3509 244 KasI G'GCGC_C Cuts at: 0 1870 3753 Size: 1870 1883 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 296 333 2102 2135 2969 3509 3753 Size: 296 37 1769 33 834 540 244 Fragments arranged by size: 1769 834 540 296 244 37 33 MscI TGG'CCA Cuts at: 0 2611 3619 3753 Size: 2611 1008 134 Fragments arranged by size: 2611 1008 134 MslI CAynn'nnrTG Cuts at: 0 3390 3753 Size: 3390 363 MspA1I CmG'CkG Cuts at: 0 478 701 974 1331 1476 1729 1789 1822 Size: 478 223 273 357 145 253 60 33 Cuts at: 1822 1900 1906 2209 2841 3753 Size: 78 6 303 632 912 Fragments arranged by size: 912 632 478 357 303 273 253 223 145 78 60 33 6 NarI GG'CG_CC Cuts at: 0 1871 3753 Size: 1871 1882 NcoI C'CATG_G Cuts at: 0 677 3140 3753 Size: 677 2463 613 Fragments arranged by size: 2463 677 613 NotI GC'GGCC_GC Cuts at: 0 1901 3753 Size: 1901 1852 NsiI A_TGCA'T Cuts at: 0 153 2733 3753 Size: 153 2580 1020 Fragments arranged by size: 2580 1020 153 PasI CC'CwG_GG Cuts at: 0 1693 2580 3753 Size: 1693 887 1173 Fragments arranged by size: 1693 1173 887 PflMI CCAn_nnn'nTGG Cuts at: 0 1147 3543 3753 Size: 1147 2396 210 Fragments arranged by size: 2396 1147 210 PfoI T'CCnGG_A Cuts at: 0 26 992 1586 2179 2401 3753 Size: 26 966 594 593 222 1352 Fragments arranged by size: 1352 966 594 593 222 26 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 424 456 2281 2313 3753 Size: 424 32 1825 32 1440 Fragments arranged by size: 1825 1440 424 32 32 PpuMI rG'GwC_Cy Cuts at: 0 36 581 1853 3753 Size: 36 545 1272 1900 Fragments arranged by size: 1900 1272 545 36 PshAI GACnn'nnGTC Cuts at: 0 3514 3753 Size: 3514 239 PspOMI G'GGCC_C Cuts at: 0 2234 3753 Size: 2234 1519 PspXI vC'TCGA_Gb Cuts at: 0 40 3753 Size: 40 3713 PstI C_TGCA'G Cuts at: 0 1285 2505 2846 3753 Size: 1285 1220 341 907 Fragments arranged by size: 1285 1220 907 341 PvuII CAG'CTG Cuts at: 0 478 974 1476 2841 3753 Size: 478 496 502 1365 912 Fragments arranged by size: 1365 912 502 496 478 SacI G_AGCT'C Cuts at: 0 3188 3753 Size: 3188 565 SacII CC_GC'GG Cuts at: 0 1730 1790 1823 1901 1907 3753 Size: 1730 60 33 78 6 1846 Fragments arranged by size: 1846 1730 78 60 33 6 SalI G'TCGA_C Cuts at: 0 3507 3753 Size: 3507 246 SapI GCTCTTCn'nnn_ Cuts at: 0 2604 3753 Size: 2604 1149 ScaI AGT'ACT Cuts at: 0 1561 3706 3753 Size: 1561 2145 47 Fragments arranged by size: 2145 1561 47 SexAI A'CCwGG_T Cuts at: 0 3695 3753 Size: 3695 58 SfcI C'TryA_G Cuts at: 0 450 1281 2240 2501 2559 2842 3753 Size: 450 831 959 261 58 283 911 Fragments arranged by size: 959 911 831 450 283 261 58 SfoI GGC'GCC Cuts at: 0 1872 3753 Size: 1872 1881 SmlI C'TyrA_G Cuts at: 0 40 1183 3753 Size: 40 1143 2570 Fragments arranged by size: 2570 1143 40 StyI C'CwwG_G Cuts at: 0 677 905 1672 2060 2230 2279 2456 3140 Size: 677 228 767 388 170 49 177 684 Cuts at: 3140 3620 3753 Size: 480 133 Fragments arranged by size: 767 684 677 480 388 228 177 170 133 49 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 548 1402 2693 3753 Size: 548 854 1291 1060 Fragments arranged by size: 1291 1060 854 548 TatI w'GTAC_w Cuts at: 0 1559 3287 3704 3753 Size: 1559 1728 417 49 Fragments arranged by size: 1728 1559 417 49 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2253 2285 3518 3550 3753 Size: 2253 32 1233 32 203 Fragments arranged by size: 2253 1233 203 32 32 Tth111I GACn'n_nGTC Cuts at: 0 2079 3753 Size: 2079 1674 XhoI C'TCGA_G Cuts at: 0 40 3753 Size: 40 3713 XmnI GAAnn'nnTTC Cuts at: 0 2894 3753 Size: 2894 859 ZraI GAC'GTC Cuts at: 0 1584 3753 Size: 1584 2169 Enzymes that do cut: AarI AatII AccI AcuI AflII AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrDI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NarI NcoI NotI NsiI PasI PflMI PfoI PpiI PpuMI PshAI PspOMI PspXI PstI PvuII SacI SacII SalI SapI ScaI SexAI SfcI SfoI SmlI StyI TaqII TatI TstI Tth111I XhoI XmnI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflIII AgeI AleI AscI AseI AsiSI BaeI BmtI BpuEI BsaAI BsaBI BsiWI BsmI BsmBI BspHI BsrBI BsrGI BssHII BssSI BstBI BstZ17I CspCI DraI DraIII EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI NruI NspI PacI PciI PmeI PmlI PsiI PsrI PvuI RsrII SanDI SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI XcmI XmaI