################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AviII AvrII BanI BbeI BbrPI BciVI BfrI BglI BglII BlnI BsaBI BsaHI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I ClaI CpoI DraII DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI Hin1I KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I SspBI StuI TaqI Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Aor51HI 1472 AGCGCT AGC^GCT AseI 1098 ATTAAT AT^TAAT AsnI 1098 ATTAAT AT^TAAT BalI 53 TGGCCA TGG^CCA BamHI 114 GGATCC G^GATCC BanII 244 GAGCTC G[GA]GC[CT]^C BbvCI 1717 GCTGAGG CCTCAGC(-5/-2) BclI 690 TGATCA T^GATCA BpmI 1691 CTCCAG CTGGAG(16/14) BsaAI 446 TACGTA [CT]AC^GT[GA] BsaI 1592 GGTCTC GGTCTC(1/5) BsaWI 117 TCCGGT [AT]^CCGG[AT] BseRI 25 CTCCTC GAGGAG(10/8) BsmAI 1593 GTCTC GTCTC(1/5) BsrFI 1985 GCCGGT [GA]^CCGG[CT] BstXI 101 CCACCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 114 GGATCC [GA]^GATC[CT] Cac8I 1833 GCCTGC GC[ACGT]^[ACGT]GC Cfr10I 1985 GCCGGT [GA]^CCGG[CT] DsaI 932 CCATGG C^C[GA][CT]GG EaeI 53 TGGCCA [CT]^GGCC[GA] Eco47III 1472 AGCGCT AGC^GCT FbaI 690 TGATCA T^GATCA HgaI 581 GACGC GACGC(5/10) HincII 1824 GTTAAC GT[CT]^[GA]AC HindII 1824 GTTAAC GT[CT]^[GA]AC HindIII 10 AAGCTT A^AGCTT HpaI 1824 GTTAAC GTT^AAC MaeII 447 ACGT A^CGT MflI 114 GGATCC [GA]^GATC[CT] MluNI 53 TGGCCA TGG^CCA MscI 53 TGGCCA TGG^CCA MspA1I 106 CAGCTG C[AC]G^C[GT]G NcoI 932 CCATGG C^CATGG PshAI 379 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1098 ATTAAT AT^TAAT PvuII 106 CAGCTG CAG^CTG SacI 244 GAGCTC GAGCT^C ScaI 541 AGTACT AGT^ACT SfcI 1430 CTGTAG C^T[GA][CT]AG SnaBI 446 TACGTA TAC^GTA SspI 1742 AATATT AAT^ATT SwaI 1548 ATTTAAAT ATTT^AAAT XcmI 101 CCACCCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 114 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AspHI 244 GAGCTC G[AT]GC[AT]^C 598 GAGCAC AvaI 187 CCCGAG C^[CT]CG[GA]G 961 CCCGAG AvaII 39 GGTCC G^G[AT]CC 504 GGTCC BcgI 1966 CGACATTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 274 GCATCCGAATCG BcnI 162 CCGGG CC^[GC]GG 204 CCGGG BlpI 126 GCTGAGC GC^T[ACGT]AGC 1475 GCTTAGC BmyI 244 GAGCTC G[AGT]GC[ACT]^C 598 GAGCAC Bpu1102I 126 GCTGAGC GC^T[ACGT]AGC 1475 GCTTAGC BsgI 752 CTGCAC GTGCAG(16/14) 1846 CTGCAC BsiHKAI 244 GAGCTC G[AT]GC[AT]^C 598 GAGCAC BsoBI 187 CCCGAG C^[CT]CG[GA]G 961 CCCGAG Bsp1286I 244 GAGCTC G[AGT]GC[ACT]^C 598 GAGCAC CelII 126 GCTGAGC GC^T[ACGT]AGC 1475 GCTTAGC DraI 607 TTTAAA TTT^AAA 1549 TTTAAA HaeII 123 GGCGCT [GA]GCGC^[CT] 1472 AGCGCT MaeIII 227 GTGAC ^GT[ACGT]AC 1706 GTCAC MwoI 622 GCTCATGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1976 GCCCTGTTTGC NciI 162 CCGGG CC^[GC]GG 204 CCGGG NdeI 1781 CATATG CA^TATG 2015 CATATG PflMI 71 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 105 CCAGCTGGTGG PleI 1612 GACTC GAGTC(4/5) 1562 GAGTC SmlI 259 CTCAAG C^T[CT][GA]AG 1595 CTCAAG Tsp45I 227 GTGAC ^GT[GC]AC 1706 GTCAC Van91I 71 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 105 CCAGCTGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI BbsI BbvI BfaI BpuAI BsaJI BsiYI BslI BsmFI BsrI BssKI BstF5I BstNI CfoI Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MboI MboII MnlI MseI MslI MspI MvaI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI StyI TfiI Tru9I TseI Tsp509I TspRI