(Circular) (Six-base) MAPSORT of: pFN21AE3689.seq Check: 1651 from: 1 to: 6760 pFN21AE3689 6760 bp With 182 enzymes: SgfI * March 28, 2012 14:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4570 278 Size: 53 83 186 936 3034 2468 Fragments arranged by size: 3034 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4103 1937 Size: 2166 4594 Acc65I G'GTAC_C Cuts at: 4081 4081 Size: 6760 AclI AA'CG_TT Cuts at: 1563 4887 5260 1563 Size: 3324 373 3063 Fragments arranged by size: 3324 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2033 2168 2904 2915 3597 4823 6207 6475 Size: 401 135 736 11 682 1226 1384 268 Cuts at: 6475 1632 Size: 1917 Fragments arranged by size: 1917 1384 1226 736 682 401 268 135 11 AfeI AGC'GCT Cuts at: 3479 3479 Size: 6760 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6563 AflIII A'CryG_T Cuts at: 1203 5659 6636 1203 Size: 4456 977 1327 Fragments arranged by size: 4456 1327 977 AgeI A'CCGG_T Cuts at: 5591 5591 Size: 6760 AhdI GACnn_n'nnGTC Cuts at: 5489 5489 Size: 6760 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6760 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4038 1184 Size: 2854 3906 AlwNI CAG_nnn'CTG Cuts at: 4503 6075 4503 Size: 1572 5188 ApaLI G'TGCA_C Cuts at: 4817 5973 4817 Size: 1156 5604 ApoI r'AATT_y Cuts at: 1493 2406 2889 2918 3883 4069 4295 4447 6556 Size: 913 483 29 965 186 226 152 2109 Cuts at: 6556 1493 Size: 1697 Fragments arranged by size: 2109 1697 965 913 483 226 186 152 29 AseI AT'TA_AT Cuts at: 160 1032 3104 5314 160 Size: 872 2072 2210 1606 Fragments arranged by size: 2210 2072 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6760 AvaI C'yCGr_G Cuts at: 1870 1943 2192 2966 4085 1870 Size: 73 249 774 1119 4545 Fragments arranged by size: 4545 1119 774 249 73 BamHI G'GATC_C Cuts at: 2119 4090 4457 2119 Size: 1971 367 4422 Fragments arranged by size: 4422 1971 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4081 5536 618 Size: 325 217 627 2294 1455 1842 Fragments arranged by size: 2294 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2253 4079 1391 Size: 862 1826 4072 Fragments arranged by size: 4072 1826 862 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2156 2346 3462 928 Size: 971 257 190 1116 4226 Fragments arranged by size: 4226 1116 971 257 190 BbvCI CC'TCA_GC Cuts at: 3723 3723 Size: 6760 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2268 2302 3960 3994 4951 4985 1903 Size: 34 331 34 1658 34 957 34 3678 Fragments arranged by size: 3678 1658 957 331 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4652 5862 4652 Size: 1210 5550 BclI T'GATC_A Cuts at: 1548 2695 1548 Size: 1147 5613 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5371 136 Size: 107 122 71 631 605 3699 1525 Fragments arranged by size: 3699 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6755 6755 Size: 6760 BlpI GC'TnA_GC Cuts at: 2132 3481 4177 2132 Size: 1349 696 4715 Fragments arranged by size: 4715 1349 696 Bme1580I G_kGCm'C Cuts at: 1790 4821 5977 1790 Size: 3031 1156 2573 Fragments arranged by size: 3031 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6760 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2422 4491 5449 Size: 416 53 83 399 24 1005 2069 958 Cuts at: 5449 442 Size: 1753 Fragments arranged by size: 2069 1753 1005 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6760 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3681 5420 1239 Size: 2442 1739 2579 Fragments arranged by size: 2579 2442 1739 Bpu10I CC'TnA_GC Cuts at: 3723 3723 Size: 6760 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2249 3585 4885 5750 6048 2249 Size: 1336 1300 865 298 2961 Fragments arranged by size: 2961 1336 1300 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3603 5423 882 Size: 617 2104 1820 2219 Fragments arranged by size: 2219 2104 1820 617 BsaAI yAC'GTr Cuts at: 493 1204 2453 4551 493 Size: 711 1249 2098 2702 Fragments arranged by size: 2702 2098 1249 711 BsaBI GATnn'nnATC Cuts at: 6754 6754 Size: 6760 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4567 4949 5584 275 Size: 53 83 186 936 3034 382 635 1451 Fragments arranged by size: 3034 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2122 5193 5591 5865 6012 6423 2122 Size: 3071 398 274 147 411 2459 Fragments arranged by size: 3071 2459 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6535 Fragments arranged by size: 6535 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2021 1186 Size: 835 5925 BseYI C'CCAG_C Cuts at: 2109 2505 2743 3253 3962 5963 6459 2109 Size: 396 238 510 709 2001 496 2410 Fragments arranged by size: 2410 2001 709 510 496 396 238 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2742 3836 1163 Size: 573 1006 1094 4087 Fragments arranged by size: 4087 1094 1006 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4210 4971 5999 1540 Size: 275 186 2209 761 1028 2301 Fragments arranged by size: 2301 2209 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2253 2607 4821 4906 5977 2253 Size: 354 2214 85 1071 3036 Fragments arranged by size: 3036 2214 1071 354 85 BsmI GAATG_Cn' Cuts at: 4271 4364 4271 Size: 93 6667 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2253 2607 4079 4821 4906 5977 1391 Size: 399 463 354 1472 742 85 1071 2174 Fragments arranged by size: 2174 1472 1071 742 463 399 354 85 BspEI T'CCGG_A Cuts at: 6423 6423 Size: 6760 BspHI T'CATG_A Cuts at: 4649 6379 4649 Size: 1730 5030 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4115 844 Size: 3271 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4647 1394 Size: 429 2824 3507 Fragments arranged by size: 3507 2824 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5255 5429 65 Size: 1183 4007 174 1396 Fragments arranged by size: 4007 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3990 5404 5591 1700 Size: 110 2180 1414 187 2869 Fragments arranged by size: 2869 2180 1414 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6760 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6760 BssSI C'ACGA_G Cuts at: 4820 5832 4820 Size: 1012 5748 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6760 BstBI TT'CG_AA Cuts at: 5564 5564 Size: 6760 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6760 BstXI CCAn_nnnn'nTGG Cuts at: 2113 2113 Size: 6760 BstYI r'GATC_y Cuts at: 1972 2119 4090 4457 4842 4859 6311 6397 6409 Size: 147 1971 367 385 17 1452 86 12 Cuts at: 6409 6755 1972 Size: 346 1977 Fragments arranged by size: 1977 1971 1452 385 367 346 147 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2937 513 Size: 552 1872 4336 Fragments arranged by size: 4336 1872 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6021 Fragments arranged by size: 6021 683 56 BtsI GCAGTG_nn' Cuts at: 4276 5070 5090 4276 Size: 794 20 5946 Fragments arranged by size: 5946 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6760 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2290 2325 529 Size: 35 1726 35 4964 Fragments arranged by size: 4964 1726 35 35 DraI TTT'AAA Cuts at: 2614 3556 4064 2614 Size: 942 508 5310 Fragments arranged by size: 5310 942 508 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5767 809 Size: 507 4451 1802 Fragments arranged by size: 4451 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 4207 5096 Size: 54 1425 50 275 18 228 2149 889 Cuts at: 5096 6725 8 Size: 1629 43 Fragments arranged by size: 2149 1629 1425 889 275 228 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4207 1537 Size: 275 2395 4090 Fragments arranged by size: 4090 2395 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2494 2776 3250 3308 3407 3573 4690 1353 Size: 1141 282 474 58 99 166 1117 3423 Fragments arranged by size: 3423 1141 1117 474 282 166 99 58 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5347 5721 5867 387 Size: 1064 138 3758 374 146 1280 Fragments arranged by size: 3758 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2251 2251 Size: 6760 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2033 2168 2904 2915 3597 3681 4823 Size: 393 401 135 736 11 682 84 1142 Cuts at: 4823 5420 6207 6475 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1142 787 736 682 597 401 393 268 135 84 11 EcoRI G'AATT_C Cuts at: 4069 4447 4069 Size: 378 6382 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2585 2585 Size: 6760 FspI TGC'GCA Cuts at: 5266 5266 Size: 6760 HaeII r_GCGC'y Cuts at: 1678 2132 3481 5907 1678 Size: 454 1349 2426 2531 Fragments arranged by size: 2531 2426 1349 454 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2493 2505 2525 2537 2611 Size: 32 135 32 921 12 20 12 74 Cuts at: 2611 2643 3572 3604 4040 4072 5401 5433 5475 Size: 32 929 32 436 32 1329 32 42 Cuts at: 5475 5507 1373 Size: 32 2626 Fragments arranged by size: 2626 1329 929 921 436 135 74 42 32 32 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 3831 4104 4350 669 Size: 988 281 1893 273 246 3079 Fragments arranged by size: 3079 1893 988 281 273 246 HindIII A'AGCT_T Cuts at: 748 2015 748 Size: 1267 5493 HpaI GTT'AAC Cuts at: 3831 4350 3831 Size: 519 6241 KpnI G_GTAC'C Cuts at: 4085 4085 Size: 6760 MfeI C'AATT_G Cuts at: 4359 4359 Size: 6760 MluI A'CGCG_T Cuts at: 6636 6636 Size: 6760 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3570 5874 6058 1305 Size: 2265 2304 184 2007 Fragments arranged by size: 2304 2265 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2060 10 Size: 54 1425 343 228 4710 Fragments arranged by size: 4710 1425 343 228 54 MslI CAynn'nnrTG Cuts at: 518 1166 2325 3717 3950 4718 5077 5236 518 Size: 648 1159 1392 233 768 359 159 2042 Fragments arranged by size: 2042 1392 1159 768 648 359 233 159 MspA1I CmG'CkG Cuts at: 1606 2113 4176 4853 6001 6246 1606 Size: 507 2063 677 1148 245 2120 Fragments arranged by size: 2120 2063 1148 677 507 245 NcoI C'CATG_G Cuts at: 513 1065 2937 513 Size: 552 1872 4336 Fragments arranged by size: 4336 1872 552 NdeI CA'TA_TG Cuts at: 387 3787 4021 387 Size: 3400 234 3126 Fragments arranged by size: 3400 3126 234 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6760 NotI GC'GGCC_GC Cuts at: 4207 4207 Size: 6760 NspI r_CATG'y Cuts at: 1132 1229 4118 5663 1132 Size: 97 2889 1545 2229 Fragments arranged by size: 2889 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6760 PciI A'CATG_T Cuts at: 5659 5659 Size: 6760 PflMI CCAn_nnn'nTGG Cuts at: 1108 2082 2116 1108 Size: 974 34 5752 Fragments arranged by size: 5752 974 34 PfoI T'CCnGG_A Cuts at: 2208 3697 2208 Size: 1489 5271 PmeI GTTT'AAAC Cuts at: 4064 4064 Size: 6760 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4829 4861 6342 6374 4829 Size: 32 1481 32 5215 Fragments arranged by size: 5215 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2388 1613 Size: 775 5985 PsiI TTA'TAA Cuts at: 2658 4005 4330 2658 Size: 1347 325 5088 Fragments arranged by size: 5088 1347 325 PstI C_TGCA'G Cuts at: 830 4112 830 Size: 3282 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6760 PvuII CAG'CTG Cuts at: 2113 2113 Size: 6760 SacI G_AGCT'C Cuts at: 2253 2253 Size: 6760 SalI G'TCGA_C Cuts at: 1936 4102 1936 Size: 2166 4594 SbfI CC_TGCA'GG Cuts at: 4112 4112 Size: 6760 ScaI AGT'ACT Cuts at: 2548 5008 6720 2548 Size: 2460 1712 2588 Fragments arranged by size: 2588 2460 1712 SfcI C'TryA_G Cuts at: 826 1045 3435 4108 5243 5924 6115 826 Size: 219 2390 673 1135 681 191 1471 Fragments arranged by size: 2390 1471 1135 681 673 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6760 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6760 SmaI CCC'GGG Cuts at: 1872 4087 1872 Size: 2215 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2264 3600 4864 5765 6027 820 Size: 197 926 321 1336 1264 901 262 1553 Fragments arranged by size: 1553 1336 1264 926 901 321 262 197 SnaBI TAC'GTA Cuts at: 493 2453 4551 493 Size: 1960 2098 2702 Fragments arranged by size: 2702 2098 1960 SpeI A'CTAG_T Cuts at: 152 152 Size: 6760 SphI G_CATG'C Cuts at: 1132 4118 1132 Size: 2986 3774 SspI AAT'ATT Cuts at: 5 52 3749 4684 5 Size: 47 3697 935 2081 Fragments arranged by size: 3697 2081 935 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6760 StyI C'CwwG_G Cuts at: 513 1065 2394 2937 3277 4199 513 Size: 552 1329 543 340 922 3074 Fragments arranged by size: 3074 1329 922 552 543 340 SwaI ATTT'AAAT Cuts at: 3556 3556 Size: 6760 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2123 3267 4787 4804 4957 5142 5587 Size: 1255 543 1144 1520 17 153 185 445 Cuts at: 5587 325 Size: 1498 Fragments arranged by size: 1520 1498 1255 1144 543 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2546 3269 3857 Size: 274 80 33 51 1443 569 723 588 Cuts at: 3857 5006 6718 96 Size: 1149 1712 138 Fragments arranged by size: 1712 1443 1149 723 588 569 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2677 2709 6720 6752 2677 Size: 32 4011 32 2685 Fragments arranged by size: 4011 2685 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6760 XbaI T'CTAG_A Cuts at: 4096 4096 Size: 6760 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2113 2113 Size: 6760 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6760 XmaI C'CCGG_G Cuts at: 1870 4085 1870 Size: 2215 4545 XmnI GAAnn'nnTTC Cuts at: 4889 4889 Size: 6760 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4568 276 Size: 53 83 186 936 3034 2468 Fragments arranged by size: 3034 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI